##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478976
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7493506
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.25307699760299	39.0	39.0	39.0	37.0	39.0
2	39.997963036261	41.0	40.0	41.0	38.0	41.0
3	40.04119300097978	41.0	40.0	41.0	38.0	41.0
4	40.05911585311335	41.0	40.0	41.0	38.0	41.0
5	40.07273457844699	41.0	40.0	41.0	38.0	41.0
6	40.06074980122789	41.0	40.0	41.0	38.0	41.0
7	40.092507966231025	41.0	40.0	41.0	38.0	41.0
8	40.07037787118606	41.0	40.0	41.0	38.0	41.0
9	40.05537381300555	41.0	40.0	41.0	38.0	41.0
10-14	39.997504959627705	41.0	40.0	41.0	38.0	41.0
15-19	39.88006679383456	41.0	40.0	41.0	38.0	41.0
20-24	39.72351369305636	41.0	39.8	41.0	37.4	41.0
25-29	39.52929317731913	40.0	39.0	41.0	36.8	41.0
30-34	39.46366130887198	40.0	39.0	41.0	36.0	41.0
35-39	39.354467534956264	40.0	38.8	41.0	35.4	41.0
40-44	38.953497735239026	40.0	37.6	41.0	35.0	41.0
45-49	38.42047805126199	39.6	36.4	41.0	35.0	41.0
50-54	37.794323698412995	38.6	35.2	40.6	34.4	41.0
55-59	37.094210266863065	37.0	35.0	39.6	34.0	41.0
60-64	36.29068577512315	35.8	34.8	38.6	33.6	40.4
65-69	35.789972704365624	35.0	35.0	37.0	33.6	39.2
70-74	35.42100501420831	35.0	35.0	36.2	33.0	38.8
75-79	35.13553861169925	35.0	35.0	35.8	33.0	37.2
80-84	34.99807008895435	35.0	35.0	35.0	33.0	37.0
85-89	34.80001777539112	35.0	34.4	35.0	32.6	37.2
90-94	39.38663369160137	40.6	39.8	40.6	36.6	40.6
95-99	39.67574716885351	41.0	39.8	41.0	37.0	41.0
100-104	39.57275547586255	41.0	39.0	41.0	36.4	41.0
105-109	39.43038975449962	40.4	39.0	41.0	36.0	41.0
110-114	39.24516887863922	40.0	38.6	41.0	35.4	41.0
115-119	39.020568772021754	40.0	38.0	41.0	35.0	41.0
120-124	38.764268465107655	40.0	38.0	41.0	35.0	41.0
125-129	38.355429795895816	39.6	37.0	40.6	34.4	41.0
130-134	38.217154571145024	39.4	36.2	41.0	34.0	41.0
135-139	37.94296139431789	39.0	35.2	41.0	34.0	41.0
140-144	37.50229139333753	37.8	35.0	40.6	34.0	41.0
145-149	36.835973699225235	36.6	35.0	39.4	34.0	41.0
150-154	36.136678499774334	35.4	35.0	38.0	33.2	40.2
155-159	35.51619610568812	35.0	35.0	36.6	33.0	39.0
160-164	35.0257116390652	35.0	35.0	36.0	33.0	37.0
165-169	34.69774602014924	35.0	34.6	35.0	33.0	36.2
170-174	34.503739733452	35.0	34.0	35.0	33.0	36.0
175-178	34.19380724787733	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	44.0
30	480.0
31	3339.0
32	17895.0
33	66792.0
34	191458.0
35	461366.0
36	1055436.0
37	2069259.0
38	2630338.0
39	971028.0
40	26070.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.974961520014798	28.4964341124168	29.24724421385664	21.281360153711763
2	22.119659342369246	28.33490758531454	28.642013498087543	20.90341957422867
3	19.9998238474754	27.642094368110197	30.454542906884974	21.903538877529424
4	20.689434291505204	26.6313124991159	30.933984706224294	21.7452685031546
5	21.67997196505881	27.540513078924604	28.775742623012512	22.003772333004072
6	21.5320038443954	27.865114140163495	28.948131889131734	21.654750126309366
7	21.56337767661759	27.840145854290366	28.553923890899668	22.04255257819237
8	21.89256937940665	28.038837895105438	28.036876196536042	22.03171652895187
9	21.862730209330586	28.265033750556817	28.24533669553344	21.626899344579158
10-14	21.872912359047955	28.116414399347917	28.02878919427035	21.981884047333786
15-19	21.897563036581275	28.06086496761329	28.102517032748086	21.939054963057348
20-24	21.94140633236298	27.881693829296992	28.11932892293674	22.057570915403286
25-29	22.03106529840638	27.817067204590213	27.887885857434426	22.263981639568982
30-34	22.105156117843904	27.83986294265995	27.894686412475018	22.16029452702113
35-39	22.145238824123183	27.837511573354316	27.836726893926556	22.180522708595948
40-44	22.136279066167425	27.774742557088768	27.82709722258179	22.261881154162015
45-49	22.232698552586733	27.74797804926025	27.811836008405145	22.20748738974787
50-54	22.22569248626744	27.82519424152059	27.754260822637626	22.194852449574338
55-59	22.20126066490105	27.73936792737605	27.76266810222078	22.296703305502124
60-64	22.144608945398854	27.8141473430461	27.835886165968237	22.205357545586804
65-69	22.16210409386474	27.821652508185085	27.791607826830322	22.224635571119848
70-74	22.11891202862852	27.78082515714273	27.78993971580192	22.310323098426824
75-79	22.101416880162635	27.818062733251963	27.839465264990782	22.24105512159462
80-84	22.104777123018252	27.85842301320637	27.779693510621062	22.257106353154317
85-89	22.05729334172816	27.82304571451601	27.75807746067061	22.36158348308522
90-94	21.093257106239534	27.87246261887714	29.389181708711675	21.645098566171654
95-99	21.564211613252272	28.148648003871614	28.34455402511494	21.942586357761176
100-104	21.795599404105452	28.21327997011151	28.02582591909828	21.96529470668476
105-109	21.78268292561677	28.102823765218243	28.038514573762836	22.075978735402156
110-114	21.809305084044905	27.94536282082022	28.11494885917238	22.1303832359625
115-119	21.930935382556	27.990723668671937	27.86149707172813	22.216843877043928
120-124	21.927911269323044	27.93732863502953	27.848183231995954	22.286576863651476
125-129	21.93522044995442	27.938915260732667	27.9088084613425	22.217055827970416
130-134	22.064346561755734	27.960850972089972	27.761688207446532	22.213114258707762
135-139	22.163552634307887	27.86112336638405	27.703878497666985	22.271445501641082
140-144	22.101249049240256	27.94927957696163	27.821292744449117	22.128178629349005
145-149	22.094030803864097	28.00225584929673	27.7904915537884	22.113221793050773
150-154	22.01260156656832	28.020059131261032	27.811473259779056	22.15586604239159
155-159	21.99008752042173	28.079658231436643	27.893002788679734	22.03725145946189
160-164	21.962166646550155	28.169505818181424	27.875319613552602	21.993007921715822
165-169	21.860393390434165	28.167887131188312	27.940235112911598	22.031484365465925
170-174	21.793126572700796	28.27104753504165	28.035578916137354	21.900246976120197
175-178	21.760391243918182	28.34696095455155	27.95746945728753	21.935178344242733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.5
7	2.0
8	3.0
9	8.5
10	23.0
11	53.5
12	107.0
13	208.0
14	365.0
15	641.5
16	1109.0
17	1806.0
18	2987.5
19	4858.5
20	7710.5
21	11728.0
22	17006.5
23	23739.5
24	31698.5
25	41226.0
26	52279.0
27	64546.0
28	78258.5
29	92531.5
30	107857.0
31	124537.0
32	141905.0
33	159107.5
34	176492.5
35	194619.0
36	212833.0
37	231234.5
38	249018.5
39	265519.0
40	279583.5
41	291102.5
42	301505.0
43	308989.0
44	312400.5
45	313308.0
46	310392.0
47	302404.0
48	290671.0
49	276091.5
50	258239.5
51	237801.5
52	216491.0
53	194710.0
54	173462.0
55	153940.0
56	136096.5
57	120260.0
58	106366.5
59	93908.5
60	82804.5
61	72991.5
62	64400.5
63	56401.0
64	48758.5
65	41527.0
66	34912.0
67	28934.0
68	23329.0
69	18431.0
70	14224.0
71	10652.5
72	7762.5
73	5443.0
74	3739.0
75	2511.5
76	1573.0
77	960.0
78	583.5
79	323.5
80	177.0
81	107.5
82	55.5
83	28.5
84	15.5
85	5.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1361.0
92-93	2861.0
94-95	4289.0
96-97	5351.0
98-99	6031.0
100-101	8033.0
102-103	8040.0
104-105	9568.0
106-107	10176.0
108-109	10933.0
110-111	11819.0
112-113	13072.0
114-115	13625.0
116-117	13734.0
118-119	15815.0
120-121	17487.0
122-123	16053.0
124-125	17672.0
126-127	18554.0
128-129	20771.0
130-131	20915.0
132-133	22231.0
134-135	106638.0
136-137	83383.0
138-139	81457.0
140-141	82639.0
142-143	84691.0
144-145	81176.0
146-147	84683.0
148-149	78819.0
150-151	81226.0
152-153	78262.0
154-155	82669.0
156-157	79047.0
158-159	83612.0
160-161	92576.0
162-163	79950.0
164-165	92088.0
166-167	96529.0
168-169	95242.0
170-171	95868.0
172-173	96499.0
174-175	101846.0
176-177	223529.0
178-179	5162686.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.98646905231857
#Duplication Level	Percentage of total
1	89.08388741629688
2	8.418073330619876
3	1.4881939992727309
4	0.4300856564038977
5	0.18262332520876587
6	0.14562721209565177
7	0.09149275758198981
8	0.049189903434685864
9	0.040034021744939516
>10	0.0659111059492943
>50	0.0
>100	0.004881271391300853
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.668977645443935E-5	0.0	1.3344888227219675E-5	0.0	0.0	0.0
2	2.668977645443935E-5	0.0	1.3344888227219675E-5	1.3344888227219675E-5	0.0	0.0
3	2.668977645443935E-5	0.0	1.3344888227219675E-5	1.3344888227219675E-5	0.0	0.0
4	4.003466468165903E-5	0.0	1.3344888227219675E-5	1.3344888227219675E-5	0.0	0.0
5	4.003466468165903E-5	0.0	1.3344888227219675E-5	1.3344888227219675E-5	1.3344888227219675E-5	0.0
6	4.003466468165903E-5	0.0	1.3344888227219675E-5	1.3344888227219675E-5	4.003466468165903E-5	0.0
7	4.003466468165903E-5	1.3344888227219675E-5	1.3344888227219675E-5	1.3344888227219675E-5	4.003466468165903E-5	0.0
8	4.003466468165903E-5	2.668977645443935E-5	1.3344888227219675E-5	2.668977645443935E-5	4.003466468165903E-5	0.0
9	4.003466468165903E-5	2.668977645443935E-5	1.3344888227219675E-5	2.668977645443935E-5	6.672444113609837E-5	0.0
10-14	4.003466468165903E-5	4.270364232710296E-5	1.8682843518107544E-5	6.405546349065445E-5	9.608319523598166E-5	0.0
15-19	4.53726199725469E-5	7.740035171787412E-5	6.672444113609839E-5	1.067591058177574E-4	1.467937704994164E-4	0.0
20-24	6.405546349065445E-5	9.341421759053772E-5	8.807626229964984E-5	1.201039940449771E-4	2.0017332340829512E-4	0.0
25-29	7.206239642698624E-5	1.3077990462675282E-4	9.341421759053772E-5	1.361178599176407E-4	2.4020798808995414E-4	0.0
30-34	1.1743501639953314E-4	1.4679377049941646E-4	1.2544194933586492E-4	1.8415945753563152E-4	3.1227038451694044E-4	0.0
35-39	1.3611785991764067E-4	1.8149047989018756E-4	1.4679377049941643E-4	2.2419412221729053E-4	3.816638032984827E-4	2.668977645443935E-6
40-44	1.9216639047196332E-4	2.0017332340829512E-4	1.5480070343574823E-4	3.1493936216238437E-4	4.3771233385280536E-4	2.668977645443935E-5
45-49	2.2953207750817843E-4	2.0017332340829512E-4	1.601386587266361E-4	3.362911833259358E-4	5.471404173160067E-4	2.9358754099883286E-5
50-54	2.402079880899542E-4	2.1618718928095873E-4	1.7081456930841186E-4	3.5497402684404335E-4	6.56568500779208E-4	5.871750819976657E-5
55-59	2.588908316080617E-4	2.6155980925350565E-4	1.7882150224474364E-4	3.7632584800759486E-4	7.206239642698625E-4	8.006932936331805E-5
60-64	2.9358754099883286E-4	2.802426527716132E-4	2.2419412221729053E-4	4.270364232710296E-4	8.193761371512881E-4	8.006932936331805E-5
65-69	3.469670939077115E-4	2.9358754099883286E-4	2.402079880899542E-4	4.670710879526886E-4	9.554939970689288E-4	8.006932936331805E-5
70-74	5.204506408615674E-4	3.015944739351647E-4	2.508838986717299E-4	5.044367749889036E-4	0.0010836049240502378	8.540728465420592E-5
75-79	0.0030533104263878617	3.496360715531555E-4	2.5889083160806167E-4	5.418024620251188E-4	0.0011796881192862193	9.341421759053772E-5
80-84	0.004043501132847562	3.656499374258191E-4	2.775736751261692E-4	5.898440596431096E-4	0.001358509621530963	9.341421759053772E-5
85-89	0.004043501132847562	4.056846021074781E-4	3.2294629509871614E-4	6.058579255157732E-4	0.0015426690790665946	9.341421759053772E-5
90-94	0.004043501132847562	4.136915350438099E-4	3.336222056804919E-4	6.325477019702127E-4	0.0016254073860753567	9.341421759053772E-5
95-99	0.004046170110493005	4.163605126892539E-4	3.4429811626226765E-4	6.93934187815423E-4	0.0016761179613387915	9.341421759053772E-5
100-104	0.004072859886947446	4.670710879526886E-4	3.576430044894873E-4	7.339688524970821E-4	0.0016814559166296792	9.341421759053772E-5
105-109	0.004083535797529221	4.804159761799084E-4	3.6031198213493126E-4	7.686655618878533E-4	0.0017001387601477866	9.341421759053772E-5
110-114	0.004083535797529221	5.017677973434598E-4	3.736568703621509E-4	8.300520477330639E-4	0.0017428424024748894	1.0142115052686952E-4
115-119	0.004099549663401885	5.311265514433431E-4	3.9767766917114637E-4	8.62079779478391E-4	0.0017855460448019927	1.067591058177574E-4
120-124	0.004147591261019875	5.818371267067779E-4	4.136915350438099E-4	9.368111535508211E-4	0.0018282496871290955	1.0942808346320134E-4
125-129	0.0041929638809924214	6.005199702248853E-4	4.136915350438099E-4	0.0010062045723323635	0.0019083190164924132	1.201039940449771E-4
130-134	0.004227660590383193	6.005199702248853E-4	4.163605126892539E-4	0.0010622531028866863	0.001999064256437507	1.201039940449771E-4
135-139	0.004265026277419408	6.11195880806661E-4	4.270364232710296E-4	0.001107625722859233	0.0021164992728370407	1.2544194933586497E-4
140-144	0.004329081740910063	6.21871791388437E-4	4.270364232710296E-4	0.001150329365186336	0.0022019065574912463	1.3344888227219675E-4
145-149	0.004369116405591721	6.485615678428762E-4	4.270364232710296E-4	0.001185026074577107	0.0022793069092091204	1.3344888227219675E-4
150-154	0.004446516757309596	6.672444113609838E-4	4.270364232710296E-4	0.0012090468733861025	0.002356707260926995	1.387868375630846E-4
155-159	0.004529255064318357	6.805892995882034E-4	4.377123338528053E-4	0.001235736649840542	0.0024047488585449855	1.4679377049941643E-4
160-164	0.004590641550163569	6.805892995882034E-4	4.403813114982493E-4	0.0012517505157132055	0.002434107612644869	1.4679377049941643E-4
165-167	0.004635124510920967	6.805892995882034E-4	4.4038131149824934E-4	0.0012855575658888287	0.002499942394565819	1.601386587266361E-4
>>END_MODULE
