##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478977
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7494401
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29259096223968	39.0	39.0	39.0	37.0	39.0
2	40.01282370665781	41.0	40.0	41.0	38.0	41.0
3	40.041249327331165	41.0	40.0	41.0	38.0	41.0
4	40.05839759575181	41.0	40.0	41.0	38.0	41.0
5	40.06237790051533	41.0	40.0	41.0	38.0	41.0
6	40.04881604280315	41.0	40.0	41.0	38.0	41.0
7	40.087760983165964	41.0	40.0	41.0	38.0	41.0
8	40.0720360439747	41.0	40.0	41.0	38.0	41.0
9	40.05226728593786	41.0	40.0	41.0	38.0	41.0
10-14	39.998380924639605	41.0	40.0	41.0	38.0	41.0
15-19	39.8826475124563	41.0	40.0	41.0	38.0	41.0
20-24	39.722310668991426	41.0	39.6	41.0	37.4	41.0
25-29	39.53093582796011	40.0	39.0	41.0	36.8	41.0
30-34	39.465200594417084	40.0	39.0	41.0	36.0	41.0
35-39	39.356199194572056	40.0	38.8	41.0	35.4	41.0
40-44	38.961625005120496	40.0	37.6	41.0	35.0	41.0
45-49	38.421726993258034	39.6	36.4	41.0	35.0	41.0
50-54	37.780785709224794	38.6	35.2	40.6	34.2	41.0
55-59	37.0710486134916	36.8	35.0	39.6	34.0	41.0
60-64	36.261668090618585	35.8	34.8	38.6	33.8	40.4
65-69	35.75397054414355	35.0	35.0	37.0	33.4	39.0
70-74	35.37504518373116	35.0	35.0	36.2	33.0	38.6
75-79	35.07529674486326	35.0	35.0	35.6	33.0	37.0
80-84	34.93131040092464	35.0	35.0	35.0	33.0	37.0
85-89	34.721900896415875	35.0	34.2	35.0	32.6	36.2
90-94	39.4113555837007	40.6	39.8	40.6	36.8	40.6
95-99	39.6961927843634	41.0	39.8	41.0	37.0	41.0
100-104	39.59357434360548	41.0	39.0	41.0	36.8	41.0
105-109	39.458562465941334	40.4	39.0	41.0	36.0	41.0
110-114	39.28231274661956	40.0	38.6	41.0	35.6	41.0
115-119	39.05807180489766	40.0	38.0	41.0	35.0	41.0
120-124	38.80081180184268	40.0	38.0	41.0	35.0	41.0
125-129	38.39897986536457	39.6	37.2	40.6	34.4	41.0
130-134	38.25688280692516	39.8	36.4	41.0	34.0	41.0
135-139	37.967970088455615	39.0	35.2	41.0	34.0	41.0
140-144	37.52796216197678	38.0	35.0	40.6	34.0	41.0
145-149	36.851426272054425	36.6	35.0	39.4	34.0	41.0
150-154	36.145330000638516	35.4	35.0	38.0	33.2	40.2
155-159	35.51760184538176	35.0	35.0	36.6	33.0	39.0
160-164	35.03013294579866	35.0	35.0	36.0	33.0	37.0
165-169	34.70357822240085	35.0	34.6	35.0	33.0	36.2
170-174	34.510564249939485	35.0	34.0	35.0	33.0	36.0
175-178	34.18723396006068	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	43.0
30	458.0
31	3460.0
32	17396.0
33	65832.0
34	193042.0
35	467708.0
36	1064197.0
37	2077320.0
38	2629232.0
39	952808.0
40	22904.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.00762956238931	28.515100806588812	29.238427460713673	21.23884217030821
2	22.155366386186166	28.303516184949274	28.608744047723096	20.932373381141467
3	19.99392613232198	27.648066870187492	30.42699476582585	21.931012231664678
4	20.701841281244494	26.562816694756524	30.975163992425813	21.760178031573172
5	21.68923173446417	27.51295266959961	28.76518083299786	22.032634762938358
6	21.544483675213	27.852726321956883	28.92026994552333	21.682520057306782
7	21.567714351020182	27.828161316694956	28.539078706890653	22.065045625394212
8	21.910023229341476	28.011364750831984	28.03410172474091	22.044510295085626
9	21.874903678092487	28.262378807859363	28.207351061145513	21.65536645290264
10-14	21.880585252910805	28.11626172658762	28.01298729544896	21.99016572505261
15-19	21.904950108754523	28.035022412064688	28.105680494011466	21.95434698516933
20-24	21.963604562926374	27.873475144978233	28.090050158778535	22.072870133316858
25-29	22.058907709902364	27.788561087136916	27.88065116878587	22.271880034174846
30-34	22.139282378938624	27.827627584913056	27.87378470941173	22.159305326736586
35-39	22.159833721200666	27.834424659155548	27.82023539973375	22.185506219910035
40-44	22.148782804656435	27.747655883372136	27.816838196941955	22.28672311502947
45-49	22.252783111018477	27.73798733214302	27.78879592912095	22.220433627717544
50-54	22.259452089633314	27.799104958488343	27.7387959357926	22.20264701608574
55-59	22.212670498949816	27.723830096628138	27.749265084694564	22.31423431972749
60-64	22.18123636565484	27.786436834644956	27.811861148075746	22.220465651624462
65-69	22.196255577997494	27.807540589301265	27.761567602267345	22.234636230433892
70-74	22.13796673009624	27.78508649323675	27.764297640331765	22.312649136335246
75-79	22.11129882161363	27.792331902176038	27.843217890262345	22.253151385947987
80-84	22.122739362358644	27.844255998578138	27.768853041090274	22.26415159797294
85-89	22.078826580002858	27.78151049029802	27.7555417704497	22.384121159249418
90-94	21.09508755096198	27.861703608202433	29.39659403063588	21.646614810199704
95-99	21.571388776996873	28.127454025741176	28.349808514347895	21.951348682914055
100-104	21.81326605378437	28.19985780611042	28.006116222033224	21.98075991807199
105-109	21.79157964862273	28.061330112431847	28.045533097310603	22.10155714163482
110-114	21.828104760448852	27.919031099320208	28.10547711368573	22.14738702654521
115-119	21.93708794479724	27.98755890502933	27.845582732515943	22.229770417657484
120-124	21.9543208053773	27.91318249107175	27.825816262539988	22.306680441010958
125-129	21.94305454950079	27.928264260136302	27.88332107485889	22.245360115504013
130-134	22.071742688657245	27.936112843879545	27.74839104535819	22.24375342210502
135-139	22.176892259027703	27.8582328899224	27.6968996213083	22.267975229741594
140-144	22.12394604239217	27.934382164151817	27.798848339694814	22.1428234537612
145-149	22.119508396178535	27.97573937810559	27.76730912659447	22.137443099121406
150-154	22.045676049145044	27.984860910299187	27.80223657281343	22.16722646774234
155-159	22.0051055783764	28.036370575973653	27.89299943156262	22.065524414087328
160-164	21.955121129367676	28.146689146154525	27.875975196256363	22.022214528221436
165-169	21.873323920771863	28.144258128372552	27.929174573904252	22.053243376951336
170-174	21.799104144644204	28.254983046033278	28.01060927419013	21.93530353513239
175-178	21.801845389324605	28.321980575920186	27.92269157369348	21.95348246106173
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5
8	5.0
9	9.0
10	22.5
11	54.0
12	103.5
13	194.0
14	346.5
15	651.5
16	1089.5
17	1763.0
18	2959.5
19	4871.0
20	7709.5
21	11620.0
22	16793.5
23	23621.0
24	31824.0
25	41389.0
26	52406.5
27	64590.0
28	77942.5
29	92597.5
30	108064.0
31	124239.5
32	141163.0
33	158410.5
34	176191.5
35	194312.0
36	212696.0
37	230838.0
38	247957.5
39	264021.5
40	278773.0
41	291067.0
42	301389.5
43	308900.5
44	312601.0
45	312680.0
46	309027.5
47	301877.5
48	290734.5
49	276144.5
50	258164.5
51	237817.5
52	216552.5
53	195103.5
54	174102.0
55	154590.0
56	137030.5
57	120683.0
58	106273.0
59	94135.0
60	83417.5
61	73597.0
62	64627.5
63	56847.5
64	49488.0
65	42157.0
66	35366.0
67	29418.5
68	23993.5
69	18896.0
70	14561.0
71	10920.5
72	7944.5
73	5540.0
74	3772.0
75	2538.0
76	1617.5
77	1002.0
78	626.5
79	362.0
80	190.0
81	99.5
82	52.5
83	30.0
84	17.0
85	7.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1156.0
92-93	2405.0
94-95	3573.0
96-97	4523.0
98-99	5506.0
100-101	6637.0
102-103	7615.0
104-105	8650.0
106-107	9417.0
108-109	10417.0
110-111	11152.0
112-113	11511.0
114-115	12521.0
116-117	12971.0
118-119	13673.0
120-121	14288.0
122-123	14931.0
124-125	16088.0
126-127	16587.0
128-129	17203.0
130-131	18072.0
132-133	19683.0
134-135	92834.0
136-137	72756.0
138-139	71254.0
140-141	73132.0
142-143	72203.0
144-145	77654.0
146-147	75099.0
148-149	80757.0
150-151	80198.0
152-153	74485.0
154-155	75809.0
156-157	74695.0
158-159	79020.0
160-161	77077.0
162-163	78213.0
164-165	80883.0
166-167	84632.0
168-169	87437.0
170-171	87686.0
172-173	97164.0
174-175	100504.0
176-177	228521.0
178-179	5333809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.73840657067991
#Duplication Level	Percentage of total
1	88.62376511472448
2	8.8127809098042
3	1.5265699455846506
4	0.417827900238678
5	0.20518010157954686
6	0.12289588001613287
7	0.08940614804001203
8	0.06488325766910485
9	0.044244136843562085
>10	0.08575411045290353
>50	0.001674344258075834
>100	0.005018150788655856
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.0029883642468556E-5	0.0	0.0
2	1.3343294547489519E-5	0.0	1.3343294547489519E-5	6.671647273744759E-5	4.0029883642468556E-5	0.0
3	1.3343294547489519E-5	1.3343294547489519E-5	1.3343294547489519E-5	8.005976728493711E-5	4.0029883642468556E-5	0.0
4	1.3343294547489519E-5	1.3343294547489519E-5	1.3343294547489519E-5	8.005976728493711E-5	9.340306183242663E-5	0.0
5	2.6686589094979037E-5	1.3343294547489519E-5	1.3343294547489519E-5	9.340306183242663E-5	9.340306183242663E-5	0.0
6	2.6686589094979037E-5	2.6686589094979037E-5	1.3343294547489519E-5	1.2008965092740567E-4	1.0674635637991615E-4	0.0
7	2.6686589094979037E-5	2.6686589094979037E-5	4.0029883642468556E-5	1.2008965092740567E-4	1.3343294547489519E-4	0.0
8	4.0029883642468556E-5	2.6686589094979037E-5	4.0029883642468556E-5	1.3343294547489519E-4	1.3343294547489519E-4	0.0
9	4.0029883642468556E-5	2.6686589094979037E-5	4.0029883642468556E-5	1.6011953456987422E-4	1.4677624002238472E-4	0.0
10-14	4.269854255196646E-5	5.604183709945598E-5	5.070451928046017E-5	1.9748075930284487E-4	1.4944489893188264E-4	0.0
15-19	5.3373178189958074E-5	8.005976728493711E-5	5.3373178189958074E-5	2.4017930185481133E-4	1.8413746475535536E-4	0.0
20-24	6.671647273744759E-5	9.874037965142243E-5	7.472244946594132E-5	2.641972320402925E-4	2.1883003057882812E-4	0.0
25-29	8.005976728493711E-5	1.2008965092740567E-4	1.0674635637991615E-4	3.0422711568276107E-4	2.802091854972799E-4	0.0
30-34	9.340306183242663E-5	1.2809562765589937E-4	1.3343294547489519E-4	3.33582363687238E-4	3.7894956514870237E-4	0.0
35-39	9.607172074192454E-5	1.4944489893188264E-4	1.6278819347937215E-4	3.9496151860568974E-4	4.910332393476143E-4	1.0674635637991615E-5
40-44	1.0674635637991615E-4	1.6011953456987422E-4	2.081553949408365E-4	4.6167799134313737E-4	6.725020451934717E-4	2.6686589094979037E-5
45-49	1.3610160438439308E-4	1.7079417020786583E-4	2.2416734839782393E-4	5.017078749856059E-4	8.272842619443503E-4	2.6686589094979037E-5
50-54	1.9214344148384908E-4	1.814688058458575E-4	2.5085393749280294E-4	5.364004408090787E-4	8.88663416862802E-4	3.736122473297066E-5
55-59	2.4551661967380715E-4	2.001494182123428E-4	2.7754052658778195E-4	6.431467971889949E-4	9.660545252382412E-4	4.536720146146436E-5
60-64	2.802091854972799E-4	2.1883003057882812E-4	2.802091854972799E-4	7.045259521074466E-4	0.0010327709979756888	5.3373178189958074E-5
65-69	3.0422711568276107E-4	2.268360073073218E-4	2.882151622257736E-4	7.605677892069026E-4	0.0011501919899935965	6.404781382794968E-5
70-74	4.536720146146436E-4	2.4017930185481133E-4	3.3091370477774014E-4	8.086036495778649E-4	0.001224914439459538	7.472244946594132E-5
75-79	0.003154354831026522	2.5085393749280294E-4	3.6560627060121286E-4	8.406275564918398E-4	0.001278287617649496	8.272842619443502E-5
80-84	0.004347245363572085	2.7754052658778195E-4	3.89624200786694E-4	9.046753703197896E-4	0.0014304011754908766	9.340306183242663E-5
85-89	0.004373931952667064	3.0422711568276107E-4	4.1364213097217514E-4	9.313619594147685E-4	0.0017266223144451437	9.340306183242663E-5
90-94	0.004381937929395558	3.2023906913974845E-4	4.269854255196646E-4	9.68723184147739E-4	0.0017586462213591187	9.340306183242663E-5
95-99	0.0044032872006715415	3.4158834041573166E-4	4.296540844291625E-4	0.0010381083157946845	0.001779995492635102	9.340306183242663E-5
100-104	0.004429973789766521	3.549316349632212E-4	4.536720146146437E-4	0.0011208367419891195	0.001830700011915562	9.340306183242663E-5
105-109	0.004451323061042504	3.816182240582003E-4	4.6701530916213316E-4	0.0011475233310840986	0.0018947478257435118	9.340306183242663E-5
110-114	0.004464666355589993	3.9763017751518766E-4	4.883645804381164E-4	0.0011875532147265673	0.0019267717326574864	9.340306183242663E-5
115-119	0.004470003673408989	4.1897944879117093E-4	4.9637055716661E-4	0.0012702816409210023	0.002001494182123428	9.340306183242663E-5
120-124	0.004488684285775474	4.510033557051458E-4	5.070451928046017E-4	0.0013369981136584497	0.002092228585046356	9.340306183242663E-5
125-129	0.00454472612287493	4.6701530916213316E-4	5.177198284425933E-4	0.001382365315119914	0.0021456017632363144	9.340306183242663E-5
130-134	0.004600767959974386	4.883645804381164E-4	5.364004408090786E-4	0.0014277325165813785	0.0022149868948832603	9.874037965142243E-5
135-139	0.004654141138164344	5.097138517140996E-4	5.550810531755639E-4	0.0014971176482283242	0.0022950466621681972	1.0674635637991615E-4
140-144	0.004680827727259323	5.203884873520913E-4	5.684243477230535E-4	0.0016198759580652276	0.0023991243596386156	1.0674635637991615E-4
145-149	0.004718188951992294	5.203884873520913E-4	5.897736189990367E-4	0.0016732491362551859	0.0024818527858330506	1.0674635637991615E-4
150-154	0.004779568106910746	5.230571462615892E-4	6.191288670035137E-4	0.0017052730431691605	0.002577924506574975	1.0674635637991615E-4
155-159	0.004798248719277231	5.337317818995807E-4	6.271348437320074E-4	0.0017453029268116292	0.002615285731307946	1.0674635637991615E-4
160-164	0.004827603967281708	5.337317818995807E-4	6.271348437320074E-4	0.0017799954926351017	0.002679333545135895	1.0674635637991615E-4
165-167	0.004865854744984511	5.38179546748744E-4	6.271348437320074E-4	0.0017880014693635955	0.0027353753822353514	1.0674635637991615E-4
>>END_MODULE
