##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478978
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7482813
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.292512722154086	39.0	39.0	39.0	37.0	39.0
2	39.997365562923996	41.0	40.0	41.0	38.0	41.0
3	40.01564278032873	41.0	40.0	41.0	38.0	41.0
4	40.0317420734689	41.0	40.0	41.0	38.0	41.0
5	40.04252384764927	41.0	40.0	41.0	38.0	41.0
6	40.02453048071628	41.0	40.0	41.0	38.0	41.0
7	40.05848255194938	41.0	40.0	41.0	38.0	41.0
8	40.039008191170886	41.0	40.0	41.0	38.0	41.0
9	40.020659476589884	41.0	40.0	41.0	38.0	41.0
10-14	39.96496806749013	41.0	40.0	41.0	38.0	41.0
15-19	39.84103045205059	41.0	40.0	41.0	38.0	41.0
20-24	39.6772841710731	40.6	39.4	41.0	37.2	41.0
25-29	39.47124181774955	40.0	39.0	41.0	36.6	41.0
30-34	39.42002629225132	40.0	39.0	41.0	36.0	41.0
35-39	39.33606436509906	40.0	38.8	41.0	35.4	41.0
40-44	38.937622869902	40.0	37.6	41.0	35.0	41.0
45-49	38.39882562881098	39.6	36.4	41.0	35.0	41.0
50-54	37.76846792776995	38.6	35.2	40.6	34.2	41.0
55-59	37.05538393114996	36.8	35.0	39.6	34.0	41.0
60-64	36.24845359626119	35.8	34.8	38.6	33.4	40.2
65-69	35.741071332398654	35.0	35.0	37.0	33.0	39.0
70-74	35.35998689262981	35.0	35.0	36.2	33.0	38.6
75-79	35.069071965315715	35.0	35.0	35.8	33.0	37.0
80-84	34.93086642683707	35.0	34.8	35.0	33.0	37.0
85-89	34.72118848887444	35.0	33.8	35.0	32.6	37.0
90-94	39.40827147720147	40.6	39.8	40.6	36.8	40.6
95-99	39.690341958784735	41.0	39.8	41.0	37.0	41.0
100-104	39.58765542384743	41.0	39.0	41.0	36.6	41.0
105-109	39.451388795058335	40.4	39.0	41.0	36.0	41.0
110-114	39.27836793654679	40.0	38.6	41.0	35.6	41.0
115-119	39.05121929919958	40.0	38.0	41.0	35.0	41.0
120-124	38.8017607478868	40.0	38.0	41.0	35.0	41.0
125-129	38.407791678316514	39.6	37.2	40.6	34.4	41.0
130-134	38.25487005354153	39.8	36.4	41.0	34.0	41.0
135-139	37.966228520476264	39.0	35.2	41.0	34.0	41.0
140-144	37.53251239086569	38.0	35.0	40.6	34.0	41.0
145-149	36.85400628970866	36.6	35.0	39.4	34.0	41.0
150-154	36.15094205410024	35.4	35.0	38.0	33.2	40.2
155-159	35.523872715757236	35.0	35.0	36.6	33.0	39.0
160-164	35.03345997348104	35.0	35.0	36.0	33.0	37.0
165-169	34.7022648882641	35.0	34.6	35.0	33.0	36.2
170-174	34.51262938784619	35.0	34.0	35.0	33.0	36.0
175-178	34.19874289855642	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	44.0
30	483.0
31	3556.0
32	18003.0
33	68466.0
34	198546.0
35	475839.0
36	1072761.0
37	2070680.0
38	2607400.0
39	944469.0
40	22564.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.02337717112535	28.515265582609107	29.23801516889437	21.22334207737117
2	22.12711984116134	28.332620366164434	28.644561877999624	20.895697914674603
3	19.952590021960994	27.68378683257219	30.448616048536824	21.91500709692999
4	20.61839578244171	26.652209536707655	30.974434347083108	21.754960333767528
5	21.696880571517692	27.548850946829756	28.785123990135798	21.969144491516758
6	21.537702465636922	27.916653803856917	28.907150292276445	21.638493438229712
7	21.526837567636665	27.88491440317966	28.542127138550704	22.046120890632974
8	21.843162457754858	28.071087704583825	28.051162042937595	22.034587794723723
9	21.796615791414272	28.283855282765984	28.315581319485066	21.603947606334675
10-14	21.84037473607853	28.13645349683334	28.048304828678734	21.97486693840939
15-19	21.873421131865783	28.060468703414077	28.128614198965014	21.937495965755126
20-24	21.92897510601962	27.890623486114112	28.12276345807386	22.05763794979241
25-29	22.027531624804737	27.82841960636996	27.887704797647622	22.256343971177685
30-34	22.07973659103869	27.85958702963712	27.91015624738985	22.15052013193434
35-39	22.119563859206423	27.862398806438165	27.85498982802323	22.163047506332177
40-44	22.118566907926205	27.792115077578444	27.83170179449894	22.25761621999641
45-49	22.22468208145787	27.784879830619847	27.79777872305509	22.192659364867197
50-54	22.217596510830887	27.841644579384784	27.748158346333124	22.192600563451204
55-59	22.200020233032685	27.752595180448854	27.770636523991715	22.276748062526753
60-64	22.149015350243285	27.815934996638298	27.83783050572024	22.19721914739818
65-69	22.166671811790568	27.825602483985634	27.788047088708485	22.219678615515313
70-74	22.100365196885182	27.81901672539458	27.77768467553579	22.302933402184447
75-79	22.076144359079937	27.832150823493784	27.85782833274064	22.233876484685638
80-84	22.09677029213479	27.865159800198136	27.79055684005467	22.247513067612406
85-89	22.048652024312247	27.81269557317549	27.78475420941296	22.353898193099305
90-94	21.078305791691292	27.8814050013515	29.401830464590322	21.638458742366883
95-99	21.556675454915737	28.137140898378675	28.37094199180204	21.93524165490355
100-104	21.787567351290818	28.222030509343632	28.027278948853247	21.963123190512306
105-109	21.75628707721765	28.10499761330918	28.07664187599385	22.062073433479316
110-114	21.802387009131525	27.9468008132412	28.125908020903957	22.124904156723314
115-119	21.92120702552959	28.016491625929916	27.869940723036144	22.192360625504346
120-124	21.93091634048539	27.95703933836246	27.828253138084147	22.283791183068
125-129	21.917123339775504	27.96032146696856	27.918844703530173	22.20371048972577
130-134	22.059255127743693	27.957213711170496	27.775048974431964	22.208482186653843
135-139	22.139051541964662	27.88659202433604	27.71069094486428	22.26366548883502
140-144	22.093439833247842	27.94407489849541	27.851427110288114	22.111058157968635
145-149	22.086798834208096	28.005446746280775	27.785982658839504	22.12177176067162
150-154	22.00024540842546	28.04441733699976	27.835646105170397	22.11969114940438
155-159	21.956799680604764	28.078094072955732	27.936034398268234	22.02907184817127
160-164	21.930596420143598	28.19670696374133	27.895177677243126	21.977518938871942
165-169	21.841139998403182	28.181272179364868	27.955071250236085	22.022516571995858
170-174	21.759088059105927	28.303651733287726	28.0707512966718	21.866508910934545
175-178	21.749633654571497	28.370220575389705	27.967215516570544	21.91293025346825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	0.5
9	9.5
10	26.5
11	55.5
12	98.0
13	183.0
14	346.5
15	611.0
16	1067.5
17	1845.0
18	3039.5
19	4891.0
20	7849.5
21	11787.5
22	16865.0
23	23737.0
24	31950.0
25	41356.0
26	52346.5
27	64862.5
28	78426.0
29	92863.5
30	108738.5
31	125206.0
32	141784.0
33	159017.0
34	177031.5
35	195298.5
36	212679.5
37	230854.5
38	248822.5
39	264736.0
40	279206.5
41	291721.5
42	301656.5
43	308426.0
44	312281.5
45	312692.5
46	309049.5
47	302033.0
48	290669.0
49	274991.5
50	257800.5
51	237449.5
52	215245.0
53	193655.0
54	172441.0
55	153043.5
56	135648.5
57	119902.5
58	105541.0
59	93252.5
60	82499.5
61	72683.5
62	63819.5
63	56042.5
64	48583.0
65	41343.5
66	34947.5
67	28957.5
68	23408.5
69	18375.0
70	14058.0
71	10602.0
72	7704.5
73	5438.5
74	3769.0
75	2447.0
76	1565.5
77	957.0
78	579.5
79	335.0
80	183.5
81	105.0
82	59.5
83	28.0
84	11.5
85	6.5
86	3.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1159.0
92-93	2525.0
94-95	3694.0
96-97	4714.0
98-99	5846.0
100-101	6669.0
102-103	7820.0
104-105	8954.0
106-107	10090.0
108-109	10764.0
110-111	11443.0
112-113	12159.0
114-115	12871.0
116-117	13348.0
118-119	14191.0
120-121	14972.0
122-123	15641.0
124-125	16402.0
126-127	17041.0
128-129	17958.0
130-131	18573.0
132-133	20549.0
134-135	90413.0
136-137	74957.0
138-139	71263.0
140-141	72151.0
142-143	71291.0
144-145	75223.0
146-147	75761.0
148-149	78437.0
150-151	78019.0
152-153	74305.0
154-155	75060.0
156-157	78326.0
158-159	78204.0
160-161	81370.0
162-163	79971.0
164-165	82622.0
166-167	86526.0
168-169	87988.0
170-171	89826.0
172-173	94407.0
174-175	99430.0
176-177	229409.0
178-179	5310471.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.88414811619097
#Duplication Level	Percentage of total
1	88.89061682134414
2	8.583900815902146
3	1.50134478158961
4	0.43823814726061644
5	0.20724733796262707
6	0.12187339238521216
7	0.07381896921692645
8	0.07326285479178087
9	0.036874271336436164
>10	0.06633621071096525
>50	0.0013964669435862676
>100	0.005089930555983124
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.3363958179898388E-5	2.6727916359796776E-5	0.0
2	1.3363958179898388E-5	0.0	0.0	1.3363958179898388E-5	2.6727916359796776E-5	0.0
3	1.3363958179898388E-5	0.0	0.0	2.6727916359796776E-5	2.6727916359796776E-5	0.0
4	2.6727916359796776E-5	0.0	1.3363958179898388E-5	2.6727916359796776E-5	2.6727916359796776E-5	0.0
5	2.6727916359796776E-5	0.0	1.3363958179898388E-5	4.0091874539695164E-5	4.0091874539695164E-5	0.0
6	2.6727916359796776E-5	0.0	2.6727916359796776E-5	5.345583271959355E-5	4.0091874539695164E-5	0.0
7	2.6727916359796776E-5	0.0	2.6727916359796776E-5	6.681979089949195E-5	5.345583271959355E-5	0.0
8	2.6727916359796776E-5	0.0	4.0091874539695164E-5	6.681979089949195E-5	5.345583271959355E-5	0.0
9	2.6727916359796776E-5	0.0	4.0091874539695164E-5	6.681979089949195E-5	6.681979089949195E-5	0.0
10-14	3.207349963175613E-5	8.018374907939032E-6	4.543745781165452E-5	1.0423887380320743E-4	9.354770725928872E-5	0.0
15-19	4.0091874539695164E-5	2.672791635979678E-5	5.880141599155291E-5	1.202756236190855E-4	1.3631237343496357E-4	0.0
20-24	5.078304108361388E-5	4.81102494476342E-5	8.285654071537E-5	1.443307483429026E-4	1.7105866470269938E-4	0.0
25-29	7.21653741714513E-5	6.414699926351227E-5	1.0691166543918712E-4	1.7373145633867905E-4	2.004593726984758E-4	0.0
30-34	1.2294841525506517E-4	7.21653741714513E-5	1.5769470652280098E-4	2.0313216433445551E-4	2.2451449742229296E-4	2.6727916359796778E-6
35-39	1.5502191488682128E-4	8.285654071537E-5	1.9778658106249617E-4	2.3520566396621165E-4	2.913342883217849E-4	1.6036749815878065E-5
40-44	1.683858730667197E-4	1.0691166543918712E-4	2.1649612251435388E-4	2.8064312177786613E-4	3.501357043133378E-4	2.672791635979678E-5
45-49	1.9244099779053682E-4	1.0691166543918712E-4	2.271872890582726E-4	3.2875337122550036E-4	4.169554952128297E-4	2.672791635979678E-5
50-54	2.2451449742229296E-4	1.0691166543918712E-4	2.5124241378208975E-4	3.7151803740117525E-4	5.425767021038746E-4	4.0091874539695164E-5
55-59	2.432240388741507E-4	1.202756236190855E-4	2.539152054180694E-4	4.2497387012076877E-4	6.254332428192445E-4	7.751095744341064E-5
60-64	2.779703301418865E-4	1.3631237343496357E-4	2.539152054180694E-4	4.490289948445858E-4	6.788890755388381E-4	8.018374907939033E-5
65-69	3.180622046815816E-4	1.6571308143074003E-4	2.752975385059068E-4	4.89120869384281E-4	7.483816580743098E-4	9.62204988952684E-5
70-74	4.971392442922201E-4	1.8442262288259777E-4	2.966798715937443E-4	5.399039104678949E-4	8.392565736976187E-4	1.0691166543918712E-4
75-79	0.003081728756284568	2.0580495597043521E-4	3.23407787953541E-4	5.63959035191712E-4	9.274586976849482E-4	1.0691166543918712E-4
80-84	0.004124117494316642	2.3520566396621165E-4	3.394445377694191E-4	5.987053264594477E-4	0.0011172269038395052	1.0691166543918712E-4
85-89	0.004177573327036237	2.539152054180694E-4	3.608268708572565E-4	6.254332428192446E-4	0.001395197233981392	1.0691166543918712E-4
90-94	0.004182918910308196	2.7262474686992713E-4	3.90227578853033E-4	6.655251173589398E-4	0.0014299435252491275	1.0691166543918712E-4
95-99	0.004188264493580155	2.806431217778662E-4	4.116099119408703E-4	6.869074504467773E-4	0.0014860721496047005	1.0691166543918712E-4
100-104	0.0042069740350320125	2.886614966858052E-4	4.2230107848478907E-4	7.403632831663707E-4	0.0015448735655962539	1.0691166543918712E-4
105-109	0.004244393117935728	3.3142616286148E-4	4.2764666175674847E-4	7.96491907521944E-4	0.001606347773223786	1.0691166543918712E-4
110-114	0.004249738701207688	3.554812875852971E-4	4.2764666175674847E-4	8.232198238817406E-4	0.001641094064491522	1.0691166543918712E-4
115-119	0.004257757076115626	3.768636206731346E-4	4.2764666175674847E-4	8.740028649653547E-4	0.0016704947724872985	1.0691166543918712E-4
120-124	0.004268448242659545	4.0893712030489073E-4	4.3031945339272806E-4	9.247859060489685E-4	0.0017266233968428718	1.0691166543918712E-4
125-129	0.004300521742291301	4.3299224502870776E-4	4.7041132793242325E-4	9.916056969484605E-4	0.0018014615626503027	1.0691166543918712E-4
130-134	0.0043913966579146105	4.6506574466046395E-4	5.131759941080982E-4	0.0010504071129400134	0.0018843181033656726	1.0691166543918712E-4
135-139	0.004431488532454305	5.024848275641794E-4	5.318855355599559E-4	0.0010771350292998103	0.0019858841855329005	1.0691166543918712E-4
140-144	0.00448227157353792	5.666318268276918E-4	5.345583271959356E-4	0.001122572487111465	0.002100814225880027	1.1493004034712614E-4
145-149	0.004546418572801432	5.88014159915529E-4	5.372311188319152E-4	0.0011546459867432207	0.0021809979749594166	1.202756236190855E-4
150-154	0.004591856030613087	6.040509097314071E-4	5.479222853758339E-4	0.0011893922780109565	0.0022157442662271527	1.202756236190855E-4
155-159	0.0046854037378723755	6.334516177271836E-4	5.612862435557324E-4	0.0012401753190945705	0.002314637556758401	1.202756236190855E-4
160-164	0.004754896320407846	6.46815575907082E-4	5.612862435557324E-4	0.0012588848605464282	0.002354729431298096	1.309667901630042E-4
165-167	0.004819934250216686	6.681979089949194E-4	5.701955490089979E-4	0.0012829399852702452	0.0023921485142018115	1.336395817989839E-4
>>END_MODULE
