##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478980
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7134688
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.259832104781594	39.0	39.0	39.0	37.0	39.0
2	40.01279285092775	41.0	40.0	41.0	38.0	41.0
3	40.05744918348216	41.0	40.0	41.0	38.0	41.0
4	40.073087849111275	41.0	40.0	41.0	38.0	41.0
5	40.0869031694168	41.0	40.0	41.0	38.0	41.0
6	40.07129351696949	41.0	40.0	41.0	38.0	41.0
7	40.09842686884136	41.0	40.0	41.0	38.0	41.0
8	40.07533167533044	41.0	40.0	41.0	38.0	41.0
9	40.05817353190497	41.0	40.0	41.0	38.0	41.0
10-14	39.99991876309097	41.0	40.0	41.0	38.0	41.0
15-19	39.871437209307544	41.0	40.0	41.0	38.0	41.0
20-24	39.70020239147108	40.8	39.6	41.0	37.2	41.0
25-29	39.4753355998188	40.0	39.0	41.0	36.4	41.0
30-34	39.36285698828036	40.0	39.0	41.0	35.8	41.0
35-39	39.17984556577667	40.0	38.2	41.0	35.0	41.0
40-44	38.684039554357526	40.0	36.8	41.0	35.0	41.0
45-49	38.070638912311225	39.0	35.4	41.0	34.8	41.0
50-54	37.41095972241533	37.4	35.0	40.2	34.0	41.0
55-59	36.73623903385824	36.4	35.0	39.2	34.0	41.0
60-64	36.00228814490556	35.2	34.8	38.0	33.2	40.0
65-69	35.59100333469382	35.0	35.0	36.8	33.0	39.0
70-74	35.3038050717845	35.0	35.0	36.0	33.0	38.6
75-79	35.0802509934562	35.0	35.0	35.4	33.0	37.0
80-84	34.98016126283308	35.0	35.0	35.0	33.0	37.0
85-89	34.799232285980835	35.0	34.4	35.0	32.6	37.4
90-94	39.32721618429174	40.6	38.8	40.6	36.6	40.6
95-99	39.59554536762204	41.0	39.0	41.0	36.6	41.0
100-104	39.47943030220015	41.0	39.0	41.0	36.0	41.0
105-109	39.31969847790875	40.0	39.0	41.0	35.6	41.0
110-114	39.11700254810087	40.0	38.0	41.0	35.0	41.0
115-119	38.860767510762685	40.0	38.0	41.0	35.0	41.0
120-124	38.55496722334533	40.0	37.4	41.0	34.2	41.0
125-129	38.082551474419624	39.4	36.2	40.6	33.8	41.0
130-134	37.866407387024736	39.0	35.4	40.8	33.8	41.0
135-139	37.53537580041194	38.4	35.0	41.0	33.6	41.0
140-144	37.07732792409636	37.0	35.0	40.0	33.8	41.0
145-149	36.43849228718632	35.8	35.0	39.0	33.0	41.0
150-154	35.803777876098124	35.0	35.0	37.2	33.0	39.6
155-159	35.264744749202826	35.0	35.0	36.2	33.0	38.4
160-164	34.84970304895495	35.0	34.8	35.4	33.0	36.8
165-169	34.57427955015948	35.0	34.0	35.0	33.0	36.0
170-174	34.408109180048676	35.0	34.0	35.0	33.0	36.0
175-178	34.10585346493383	34.75	33.75	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	25.0
30	430.0
31	3233.0
32	17393.0
33	67605.0
34	201678.0
35	508275.0
36	1213849.0
37	2160488.0
38	2223479.0
39	724731.0
40	13502.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.530640162541097	27.650865742131963	28.954104229925683	21.86438986540126
2	22.73165974461672	28.10780233137034	28.116968814894218	21.043569109118717
3	20.21711390883526	27.098409909445238	30.11694414668168	22.567532035037832
4	21.148423028449177	25.932794818778344	30.511369242775576	22.407412909996903
5	22.408716400773237	26.790393637395216	28.24928013670675	22.5516098251248
6	22.193668454738315	27.27972127162393	28.230736929211197	22.295873344426553
7	22.224237415847757	27.26947555380137	27.824846720697526	22.681440309653343
8	22.541392139361946	27.384028565790125	27.448810655770796	22.62576863907714
9	22.53292645733072	27.601010723944764	27.55003442336932	22.3160283953552
10-14	22.59757959983674	27.387156943653316	27.301255499890114	22.714007956619827
15-19	22.64964354432878	27.307733708888183	27.362062643804467	22.680560102978575
20-24	22.683915540525387	27.143095815822637	27.39026009266278	22.782728550989194
25-29	22.755537453074332	27.079586381352627	27.179582344736026	22.985293820837015
30-34	22.86019514798685	27.123453751586613	27.126792369897608	22.88955873052893
35-39	22.86979612843617	27.11272868554308	27.115060952910625	22.902414233110125
40-44	22.89442789929987	27.048605348965506	27.055860046017425	23.001106705717195
45-49	22.995589996367045	27.024769688597456	27.034252934396008	22.945387380639488
50-54	23.016557977027166	27.058710906489537	26.987259989504796	22.937471126978505
55-59	22.956628236581615	27.0135989128046	26.996106907548022	23.033665943065763
60-64	22.908129970084186	27.08470223224898	27.053376405527473	22.95379139213936
65-69	22.932613171031445	27.074986320354867	27.021439479904373	22.970961028709315
70-74	22.863553388739632	27.05034894307922	27.01459124771819	23.071506420462956
75-79	22.83617447602474	27.0862832404164	27.081346794702167	22.996195488856696
80-84	22.847608753178836	27.117805291555847	27.021049834274464	23.013536120990853
85-89	22.814979435681	27.08227745908441	26.983901748752015	23.11884135648258
90-94	21.593479131545326	27.27856858700647	28.910437205091498	22.217515076356705
95-99	22.194735011119953	27.515491426696144	27.671921329473335	22.617852232710568
100-104	22.48211199703606	27.487146440002007	27.30734229130569	22.723399271656234
105-109	22.459286028352068	27.34163448736026	27.35569008179904	22.84338940248863
110-114	22.48679897149967	27.22668641216584	27.392481750225794	22.894032866108695
115-119	22.59439621681181	27.263529985824995	27.16136430210178	22.98070949526142
120-124	22.62616846466378	27.22125973620088	27.09722572095363	23.055346078181703
125-129	22.625775120195296	27.22380806211714	27.16687101456564	22.983545803121928
130-134	22.773418456718854	27.217861994647524	27.00777113563955	23.00094841299407
135-139	22.880875105475866	27.131544556843977	26.969114943533402	23.01846539414676
140-144	22.832735958108263	27.229186158990398	27.047886468166233	22.890191414735103
145-149	22.823663630034837	27.292755700215952	27.015643704342924	22.867936965406283
150-154	22.72884230415793	27.31742643366899	27.071551189129124	22.88218007304396
155-159	22.689972036446555	27.38332433558604	27.14991967403037	22.776783953937038
160-164	22.6392729467205	27.490531400149827	27.169260629341995	22.70093502378768
165-169	22.508623175150902	27.529070395443494	27.235269849710857	22.727036579694747
170-174	22.427164822910374	27.66308848307868	27.336376576441346	22.5733701175696
175-178	22.403671943305405	27.711270299982882	27.283061099253775	22.60199665745794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	3.5
9	3.5
10	5.5
11	16.5
12	39.0
13	83.0
14	146.5
15	272.0
16	485.5
17	852.0
18	1493.5
19	2547.5
20	4234.0
21	6736.0
22	10288.5
23	14953.0
24	20885.0
25	28537.0
26	37747.0
27	48249.5
28	60193.5
29	73656.5
30	88401.5
31	103784.0
32	119812.0
33	137113.5
34	155199.5
35	173444.5
36	191772.0
37	209828.0
38	227817.5
39	244054.5
40	257381.5
41	269252.0
42	278148.5
43	282751.0
44	283927.5
45	281357.5
46	275079.0
47	265387.0
48	253696.0
49	239901.0
50	224353.0
51	209759.0
52	195762.0
53	182835.5
54	170712.0
55	160081.0
56	151040.5
57	142440.0
58	134682.5
59	127035.0
60	118740.0
61	109604.0
62	99403.0
63	88530.0
64	77337.0
65	65879.5
66	55091.5
67	44640.5
68	35242.0
69	27188.0
70	20342.0
71	14936.5
72	10567.0
73	7216.0
74	4816.0
75	3134.0
76	1969.5
77	1211.0
78	717.0
79	408.0
80	210.5
81	93.5
82	48.0
83	32.0
84	20.5
85	8.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1446.0
92-93	2955.0
94-95	4653.0
96-97	6031.0
98-99	6805.0
100-101	8660.0
102-103	8979.0
104-105	10393.0
106-107	11306.0
108-109	11973.0
110-111	13075.0
112-113	14164.0
114-115	15091.0
116-117	15551.0
118-119	17254.0
120-121	19110.0
122-123	17723.0
124-125	19432.0
126-127	20442.0
128-129	22633.0
130-131	22927.0
132-133	24131.0
134-135	113726.0
136-137	88262.0
138-139	86351.0
140-141	86890.0
142-143	89418.0
144-145	86827.0
146-147	90416.0
148-149	84537.0
150-151	86901.0
152-153	83650.0
154-155	87457.0
156-157	84132.0
158-159	88998.0
160-161	98628.0
162-163	85476.0
164-165	97309.0
166-167	101094.0
168-169	100477.0
170-171	101515.0
172-173	101695.0
174-175	105590.0
176-177	230053.0
178-179	4660552.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.77592526881979
#Duplication Level	Percentage of total
1	95.68078978936283
2	3.984622473716436
3	0.2595384444271821
4	0.04696374053801032
5	0.01336546558792809
6	0.003105922834297784
7	0.005212677478584707
8	0.0010497963818264738
9	0.0010570902598875426
>10	0.004294599413024664
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1212823882417843E-4	1.4016029853022304E-5	1.4016029853022304E-5	1.4016029853022304E-5	1.4016029853022304E-5	0.0
2	1.6819235823626767E-4	2.8032059706044608E-5	2.8032059706044608E-5	4.204808955906692E-5	2.8032059706044608E-5	0.0
3	1.6819235823626767E-4	4.204808955906692E-5	2.8032059706044608E-5	4.204808955906692E-5	4.204808955906692E-5	0.0
4	1.6819235823626767E-4	7.008014926511152E-5	2.8032059706044608E-5	5.6064119412089216E-5	4.204808955906692E-5	0.0
5	1.8220838808928995E-4	8.409617911813383E-5	5.6064119412089216E-5	7.008014926511152E-5	4.204808955906692E-5	0.0
6	1.8220838808928995E-4	8.409617911813383E-5	7.008014926511152E-5	7.008014926511152E-5	5.6064119412089216E-5	0.0
7	1.8220838808928995E-4	8.409617911813383E-5	7.008014926511152E-5	8.409617911813383E-5	5.6064119412089216E-5	0.0
8	1.8220838808928995E-4	1.1212823882417843E-4	7.008014926511152E-5	8.409617911813383E-5	5.6064119412089216E-5	0.0
9	1.8220838808928995E-4	1.1212823882417843E-4	8.409617911813383E-5	9.811220897115613E-5	5.6064119412089216E-5	0.0
10-14	2.074372418247301E-4	1.345538865890141E-4	1.0091541494176059E-4	1.1212823882417843E-4	5.886732538269367E-5	0.0
15-19	2.1024044779533458E-4	1.5417632838324536E-4	1.1212823882417843E-4	1.4296350450082753E-4	7.008014926511152E-5	0.0
20-24	2.1584685973654352E-4	1.569795343538498E-4	1.3455388658901413E-4	1.794051821186855E-4	7.008014926511152E-5	0.0
25-29	2.438789194425881E-4	1.6819235823626767E-4	1.5137312241264087E-4	2.6069815526621483E-4	8.409617911813383E-5	0.0
30-34	2.7751739108984163E-4	1.8781480003049887E-4	1.6819235823626767E-4	3.055494507958862E-4	1.009154149417606E-4	0.0
35-39	3.5600715826676654E-4	2.3266609556017023E-4	1.9061800600110335E-4	3.504007463255576E-4	1.289474746478052E-4	1.1212823882417844E-5
40-44	3.924488358846245E-4	2.4107571347198363E-4	1.9622441794231227E-4	4.092680717082513E-4	1.6819235823626764E-4	1.6819235823626763E-5
45-49	4.2048089559066916E-4	2.578949492956104E-4	1.9622441794231227E-4	4.7654501500275835E-4	1.9622441794231227E-4	5.6064119412089216E-5
50-54	4.5692257320852715E-4	2.803205970604461E-4	2.074372418247301E-4	5.354123403854521E-4	2.1865006570714798E-4	7.568656120632044E-5
55-59	5.326091344148476E-4	2.9433662691346837E-4	2.5228853735440146E-4	5.494283702384743E-4	2.7471418511923717E-4	1.0932503285357399E-4
60-64	5.466251642678698E-4	2.9433662691346837E-4	2.8592700900165495E-4	5.718540180033101E-4	2.999430388546773E-4	1.5978274032445427E-4
65-69	5.662476060621011E-4	3.055494507958862E-4	3.1115586273709517E-4	6.223117254741902E-4	3.2517189259011745E-4	1.6819235823626767E-4
70-74	7.820944657986447E-4	3.4759754035495316E-4	3.4199112841374425E-4	6.587534030920483E-4	3.4199112841374425E-4	1.8220838808928995E-4
75-79	0.004333756430554497	3.5881036423737105E-4	3.700231881197889E-4	7.176207284747421E-4	3.532039522961621E-4	1.9622441794231227E-4
80-84	0.005808242771092443	3.812360120022067E-4	4.2608730753187807E-4	7.933072896810625E-4	5.129866926206165E-4	2.1024044779533458E-4
85-89	0.00587551971438695	4.2608730753187807E-4	4.541193672379227E-4	8.381585852107338E-4	8.185361434165025E-4	2.1024044779533458E-4
90-94	0.005931583833799039	4.905610448557807E-4	5.073802806794074E-4	8.68993850887383E-4	8.26945761328316E-4	2.1024044779533458E-4
95-99	0.00595681268753448	5.354123403854521E-4	5.326091344148476E-4	9.110419404464498E-4	8.26945761328316E-4	2.1024044779533458E-4
100-104	0.005998860777093547	5.718540180033101E-4	5.606411941208922E-4	9.110419404464498E-4	8.26945761328316E-4	2.1024044779533458E-4
105-109	0.006026892836799591	5.942796657681457E-4	5.802636359151233E-4	9.418772061230988E-4	8.409617911813383E-4	2.1304365376593904E-4
110-114	0.006032499248740801	6.08295695621168E-4	6.110989015917724E-4	9.67106059858539E-4	8.68993850887383E-4	2.2425647764835686E-4
115-119	0.006063334514417449	6.307213433860037E-4	6.307213433860037E-4	0.0010119573553882104	8.68993850887383E-4	2.2425647764835686E-4
120-124	0.006119398633829537	6.363277553272126E-4	6.475405792096304E-4	0.0010792342986827175	8.717970568579873E-4	2.2986288958956575E-4
125-129	0.006203494812947672	6.44737373239026E-4	6.671630210038618E-4	0.0011044631524181575	9.138451464170543E-4	2.4668212541319255E-4
130-134	0.006321229463713059	6.531469911508394E-4	6.755726389156751E-4	0.0011465112419772245	9.558932359761211E-4	2.663045672074238E-4
135-139	0.006366080759242732	6.587534030920483E-4	6.979982866805107E-4	0.001216591391242336	0.0010007445315057925	2.663045672074238E-4
140-144	0.006436160908507842	6.81179050856884E-4	7.148175225041376E-4	0.0012446234509483807	0.0010680214748002997	2.775173910898417E-4
145-149	0.006509044263743558	7.120143165335331E-4	7.288335523571598E-4	0.0012782619225956342	0.0011072663583887622	2.8592700900165495E-4
150-154	0.006545485941361416	7.260303463865554E-4	7.288335523571598E-4	0.0012978843643898653	0.0011352984180948067	3.083526567664907E-4
155-159	0.006584730824949879	7.400463762395778E-4	7.484559941513911E-4	0.0013147036002134922	0.001185756125565687	3.083526567664907E-4
160-164	0.00668564623989164	7.624720240044134E-4	7.568656120632045E-4	0.0013539484838019547	0.0012137881852717317	3.083526567664907E-4
165-167	0.00677441442896078	7.84897671769249E-4	7.568656120632045E-4	0.0013735709255961858	0.0012334106270659628	3.083526567664907E-4
>>END_MODULE
