##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478981
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7144136
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29760337709136	39.0	39.0	39.0	37.0	39.0
2	40.02788468752554	41.0	40.0	41.0	38.0	41.0
3	40.05716142581832	41.0	40.0	41.0	38.0	41.0
4	40.0706905635615	41.0	40.0	41.0	38.0	41.0
5	40.07443741272562	41.0	40.0	41.0	38.0	41.0
6	40.05998821970914	41.0	40.0	41.0	38.0	41.0
7	40.092691264555995	41.0	40.0	41.0	38.0	41.0
8	40.07660534457911	41.0	40.0	41.0	38.0	41.0
9	40.055928526556606	41.0	40.0	41.0	38.0	41.0
10-14	39.99837088767627	41.0	40.0	41.0	38.0	41.0
15-19	39.869381993847824	41.0	40.0	41.0	38.0	41.0
20-24	39.69413409823105	40.8	39.6	41.0	37.2	41.0
25-29	39.47107843411716	40.0	39.0	41.0	36.4	41.0
30-34	39.359506006044676	40.0	38.8	41.0	36.0	41.0
35-39	39.17949789869622	40.0	38.2	41.0	35.0	41.0
40-44	38.68835223741541	40.0	37.0	41.0	35.0	41.0
45-49	38.0686493370227	39.0	35.4	41.0	34.8	41.0
50-54	37.395480993083	37.4	35.0	40.2	34.0	41.0
55-59	36.70904537651579	36.2	35.0	39.2	34.0	41.0
60-64	35.97282991253247	35.2	34.8	38.0	33.0	40.0
65-69	35.55504797221106	35.0	35.0	36.6	33.0	39.0
70-74	35.25879168593655	35.0	35.0	36.0	33.0	38.2
75-79	35.0219612840517	35.0	35.0	35.2	33.0	37.0
80-84	34.91281694525412	35.0	35.0	35.0	33.0	37.0
85-89	34.72219803766334	35.0	34.0	35.0	32.4	37.2
90-94	39.3558680126987	40.6	38.8	40.6	36.6	40.6
95-99	39.62146590521114	41.0	39.2	41.0	37.0	41.0
100-104	39.50413398078509	41.0	39.0	41.0	36.0	41.0
105-109	39.351665425353076	40.2	39.0	41.0	35.8	41.0
110-114	39.15768777487117	40.0	38.2	41.0	35.0	41.0
115-119	38.90011964001385	40.0	38.0	41.0	35.0	41.0
120-124	38.59098110715307	40.0	37.4	41.0	34.2	41.0
125-129	38.12252170075318	39.6	36.6	40.6	33.8	41.0
130-134	37.9006011885551	39.0	35.4	40.8	33.8	41.0
135-139	37.554229662998864	38.4	35.0	41.0	33.6	41.0
140-144	37.098118372500814	37.0	35.0	40.0	33.8	41.0
145-149	36.45153984980483	35.8	35.0	39.0	33.0	41.0
150-154	35.812345227955305	35.0	35.0	37.2	33.0	39.6
155-159	35.267285928407496	35.0	35.0	36.2	33.0	38.4
160-164	34.85374193334773	35.0	34.8	35.4	33.0	36.8
165-169	34.58015657582912	35.0	34.0	35.0	33.0	36.0
170-174	34.41533232698655	35.0	34.0	35.0	33.0	36.0
175-178	34.09929717388448	34.75	33.75	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	20.0
30	391.0
31	3178.0
32	16709.0
33	66552.0
34	203416.0
35	515894.0
36	1227844.0
37	2174188.0
38	2217710.0
39	707108.0
40	11125.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.54261900949254	27.66233733512352	28.867731521348418	21.927312134035525
2	22.743226052807504	28.04417497091321	28.123498768780436	21.08910020749885
3	20.224405022524767	27.056091877310283	30.12753396631867	22.591969133846277
4	21.175478742285982	25.905931801970176	30.527722316596435	22.390867139147407
5	22.401701199417257	26.761052701124388	28.25983435925632	22.577411740202034
6	22.22713845313135	27.215663307641403	28.234358920378895	22.322839318848352
7	22.222043365355866	27.271163370910074	27.822132725356852	22.68466053837721
8	22.56032080016394	27.37160938705534	27.403215728256015	22.66485408452471
9	22.566227742584967	27.552065078268388	27.53021499030813	22.35149218883851
10-14	22.615851657919166	27.35064114121008	27.286991736999404	22.746515463871347
15-19	22.673832077104915	27.267064904699463	27.354196504657807	22.704906513537818
20-24	22.704021871924052	27.11113282277941	27.362329048607137	22.8225162566894
25-29	22.787032609681564	27.034202596367145	27.16469003389633	23.01407476005496
30-34	22.873747084322023	27.098770236176918	27.108294131018784	22.919188548482282
35-39	22.898603833969567	27.078664235955195	27.086858368877635	22.935873561197603
40-44	22.91190985166016	27.003419307807132	27.051033183018912	23.033637657513797
45-49	23.033114151242362	26.991023127219304	27.003452901792464	22.972409819745874
50-54	23.034877835472336	27.043174989949797	26.96570725977221	22.956239914805654
55-59	22.97992087496655	27.002268713809478	26.954548457644144	23.06326195357983
60-64	22.92858646587915	27.058759799645472	27.021812014776874	22.990841719698505
65-69	22.969587924977912	27.04618165163709	26.992193317708395	22.992037105676598
70-74	22.888354868944262	27.033880654007707	26.998688154872752	23.079076322175275
75-79	22.879102525483837	27.05615626578217	27.041114558849383	23.023626649884605
80-84	22.884393578173764	27.088826416518387	26.983794821375177	23.042985183932668
85-89	22.86190520449219	27.048074112810845	26.955788635602683	23.13423204709429
90-94	21.590042573400194	27.267576788368427	28.932898452192006	22.209482186039374
95-99	22.199195578316164	27.477045160545092	27.673914274643895	22.649844986494845
100-104	22.501693672936966	27.46161441221885	27.298790264125305	22.73790165071888
105-109	22.487066307418964	27.328667247871753	27.31741620744652	22.866850237262764
110-114	22.513342544276615	27.204429835665962	27.367923701503837	22.91430391855358
115-119	22.624076350966494	27.25658686266197	27.135116674870307	22.98422011150123
120-124	22.635921534905037	27.1976903395177	27.086072729100202	23.08031539647706
125-129	22.638584897582373	27.21540189573528	27.13427369363289	23.01173951304946
130-134	22.791552992893504	27.19165445256026	26.98533805787636	23.03145449666988
135-139	22.91401103965342	27.113277697860855	26.91857985564151	23.054131406844213
140-144	22.866901303240898	27.215301184786405	27.021875653539514	22.895921858433187
145-149	22.842521898986078	27.285207200356083	26.98007394500528	22.892196955652558
150-154	22.760186419278114	27.291429734331086	27.043747908748685	22.904635937642112
155-159	22.70742850509971	27.36911616940285	27.127001834080826	22.796453491416607
160-164	22.650986598665728	27.467287644211645	27.149236730656124	22.732489026466503
165-169	22.553563523747464	27.481492109790345	27.205132499817374	22.759811866644817
170-174	22.466640609155885	27.63066838275291	27.29363105179607	22.609059956295138
175-178	22.41795193007341	27.705987178036455	27.263425794158437	22.6126350977317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.5
7	0.5
8	2.5
9	5.5
10	8.0
11	19.0
12	45.0
13	80.5
14	145.0
15	258.5
16	454.5
17	814.0
18	1470.0
19	2515.0
20	4206.5
21	6648.0
22	10195.0
23	14983.0
24	21021.5
25	28542.0
26	37822.0
27	48277.0
28	60131.0
29	73519.0
30	87946.5
31	103440.5
32	119465.0
33	136741.5
34	154635.5
35	172928.0
36	192057.0
37	209888.0
38	226910.0
39	242633.0
40	256607.0
41	268653.5
42	277478.5
43	282143.0
44	282708.5
45	280386.5
46	274633.0
47	265463.5
48	253310.0
49	239621.5
50	225150.0
51	210863.0
52	196962.5
53	183878.5
54	171580.5
55	160814.0
56	151928.0
57	143283.5
58	135292.0
59	127860.0
60	119940.0
61	110782.0
62	100744.0
63	90085.5
64	78448.5
65	66735.5
66	55534.5
67	45189.5
68	36018.5
69	27902.0
70	20983.0
71	15176.5
72	10662.5
73	7381.0
74	4954.5
75	3156.5
76	2018.0
77	1237.0
78	693.0
79	415.5
80	249.0
81	136.0
82	63.5
83	30.0
84	15.5
85	5.5
86	3.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1301.0
92-93	2704.0
94-95	3890.0
96-97	5051.0
98-99	6278.0
100-101	7278.0
102-103	8318.0
104-105	9467.0
106-107	10487.0
108-109	11520.0
110-111	12460.0
112-113	13129.0
114-115	13862.0
116-117	14565.0
118-119	15249.0
120-121	16119.0
122-123	16943.0
124-125	17511.0
126-127	18275.0
128-129	19234.0
130-131	19872.0
132-133	22019.0
134-135	101381.0
136-137	78516.0
138-139	77195.0
140-141	79023.0
142-143	78864.0
144-145	83081.0
146-147	81344.0
148-149	86313.0
150-151	85799.0
152-153	80699.0
154-155	81505.0
156-157	81435.0
158-159	85050.0
160-161	83946.0
162-163	84290.0
164-165	87210.0
166-167	90300.0
168-169	93252.0
170-171	94394.0
172-173	103462.0
174-175	105784.0
176-177	236658.0
178-179	4819103.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.70141812870231
#Duplication Level	Percentage of total
1	95.53769696253929
2	4.108358890566823
3	0.28284722417747304
4	0.04491993584716469
5	0.010280588594812099
6	0.006211458047107871
7	0.001042460070798469
8	0.001049710039813808
9	0.003170978715241787
>10	0.0044217914014823235
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.598997555477667E-5	0.0	1.3997493888694168E-5	0.0	0.0	0.0
2	6.998746944347084E-5	0.0	1.3997493888694168E-5	1.3997493888694168E-5	0.0	0.0
3	6.998746944347084E-5	0.0	2.7994987777388336E-5	1.3997493888694168E-5	1.3997493888694168E-5	0.0
4	6.998746944347084E-5	0.0	2.7994987777388336E-5	2.7994987777388336E-5	1.3997493888694168E-5	0.0
5	8.398496333216502E-5	1.3997493888694168E-5	2.7994987777388336E-5	2.7994987777388336E-5	1.3997493888694168E-5	0.0
6	9.798245722085918E-5	1.3997493888694168E-5	2.7994987777388336E-5	4.199248166608251E-5	1.3997493888694168E-5	0.0
7	9.798245722085918E-5	1.3997493888694168E-5	2.7994987777388336E-5	6.998746944347084E-5	1.3997493888694168E-5	0.0
8	9.798245722085918E-5	2.7994987777388336E-5	2.7994987777388336E-5	6.998746944347084E-5	2.7994987777388336E-5	0.0
9	9.798245722085918E-5	5.598997555477667E-5	2.7994987777388336E-5	8.398496333216502E-5	2.7994987777388336E-5	0.0
10-14	1.0358145477633684E-4	5.598997555477667E-5	2.7994987777388336E-5	9.798245722085917E-5	4.759147922156017E-5	0.0
15-19	1.1477944988729218E-4	6.7187970665732E-5	2.7994987777388336E-5	1.2317794622050871E-4	5.598997555477667E-5	0.0
20-24	1.3157644255372517E-4	9.798245722085917E-5	3.6393484110604837E-5	1.3437594133146403E-4	5.598997555477667E-5	0.0
25-29	1.5957143033111352E-4	1.2877694377598636E-4	4.199248166608251E-5	1.6796992666433E-4	7.278696822120967E-5	0.0
30-34	1.6796992666433003E-4	1.3997493888694167E-4	6.438847188799318E-5	2.435563936632785E-4	1.0078195599859802E-4	2.7994987777388335E-6
35-39	2.1276190710815135E-4	1.4837343522015818E-4	1.2037844744276985E-4	2.6595238388518917E-4	1.3997493888694167E-4	2.7994987777388336E-5
40-44	2.2675940099684553E-4	1.5397243277563585E-4	1.3997493888694167E-4	2.911478728848387E-4	1.567719315533747E-4	4.7591479221560166E-5
45-49	2.631528851074504E-4	1.7076942544206887E-4	1.4557393644241937E-4	3.835313325502202E-4	1.819674205530242E-4	6.998746944347084E-5
50-54	3.275413569954435E-4	1.8196742055302418E-4	1.7916792177528537E-4	4.255238142163027E-4	2.01563911997196E-4	8.398496333216502E-5
55-59	3.7233333743926487E-4	2.01563911997196E-4	2.0716290955267368E-4	4.8711278732655713E-4	2.575538875519727E-4	1.0918045233181451E-4
60-64	3.975288264389144E-4	2.0996240833041254E-4	2.18360904663629E-4	5.151077751039453E-4	3.1914286066222705E-4	1.3717544010920284E-4
65-69	4.2832331299404153E-4	2.3515789733006203E-4	2.239599022191067E-4	5.37503765325856E-4	3.9192982888343667E-4	1.8476691933076302E-4
70-74	6.466842176576706E-4	2.5195488999649505E-4	2.3515789733006203E-4	5.822957457696775E-4	4.4232080688273566E-4	1.9596491444171833E-4
75-79	0.0041012657093873915	2.5755388755197266E-4	2.3795739610780087E-4	6.466842176576706E-4	4.7031579466012407E-4	1.9596491444171833E-4
80-84	0.0057585689858087805	2.6595238388518917E-4	2.6315288510745034E-4	7.138721883234026E-4	5.990927384361104E-4	1.9596491444171833E-4
85-89	0.005803360966252602	2.7994987777388335E-4	2.7994987777388335E-4	7.362681785453133E-4	9.714260758753752E-4	1.9596491444171833E-4
90-94	0.005848152946696423	3.219423594399659E-4	2.9674687044031636E-4	7.782606602113959E-4	9.93822066097286E-4	1.9596491444171833E-4
95-99	0.005876147934473812	3.6393484110604837E-4	3.079448655512717E-4	8.174536430997394E-4	9.93822066097286E-4	1.9596491444171833E-4
100-104	0.005892944927140245	3.9192982888343667E-4	3.079448655512717E-4	8.538471272103443E-4	9.93822066097286E-4	1.9596491444171833E-4
105-109	0.005892944927140245	4.479198044382134E-4	3.219423594399659E-4	8.762431174322549E-4	9.93822066097286E-4	1.9596491444171833E-4
110-114	0.0059125414185844175	4.507193032159522E-4	3.4433834966187646E-4	9.266340954315539E-4	9.93822066097286E-4	1.9596491444171833E-4
115-119	0.005934937408806328	4.731152934378629E-4	3.975288264389144E-4	9.91022567319547E-4	9.966215648750248E-4	1.9596491444171833E-4
120-124	0.005996526381916582	5.179072738816842E-4	4.199248166608251E-4	0.0010358145477633684	0.0010190175550969355	1.9596491444171833E-4
125-129	0.006083310844026485	5.37503765325856E-4	4.227243154385638E-4	0.0010890050245404065	0.0010750075306517122	1.9596491444171833E-4
130-134	0.006142100318359002	5.571002567700278E-4	4.339223105495192E-4	0.0011309975062064887	0.0011058020172068394	1.9596491444171833E-4
135-139	0.006164496308580911	5.682982518809833E-4	4.339223105495192E-4	0.0011785889854280488	0.0011058020172068394	2.0716290955267368E-4
140-144	0.006189691797580561	6.102907335470657E-4	4.339223105495192E-4	0.0012121829707609148	0.001122599009873272	2.239599022191067E-4
145-149	0.0062316842792466435	6.158897311025434E-4	4.4232080688273566E-4	0.0012653734475379529	0.0011477944988729219	2.239599022191067E-4
150-154	0.006259679267024032	6.186892298802822E-4	4.731152934378629E-4	0.0013073659292040351	0.0011617919927616159	2.239599022191067E-4
155-159	0.006324067738912026	6.494837164354094E-4	4.899122861042959E-4	0.001329761919425946	0.0011617919927616159	2.2955889977458436E-4
160-164	0.0064192506973551455	6.858772005460142E-4	5.011102812152513E-4	0.0013549574084255954	0.0011701904890948325	2.4915539121875627E-4
165-167	0.006490171333057862	6.858772005460142E-4	5.039097799929901E-4	0.001390417726276954	0.0011804553179465417	2.5195488999649505E-4
>>END_MODULE
