##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478982
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7096999
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.28542543122804	39.0	39.0	39.0	37.0	39.0
2	39.99779991514723	41.0	40.0	41.0	38.0	41.0
3	40.01890926009712	41.0	40.0	41.0	38.0	41.0
4	40.03454319212952	41.0	40.0	41.0	38.0	41.0
5	40.04292969464981	41.0	40.0	41.0	38.0	41.0
6	40.02334761495669	41.0	40.0	41.0	38.0	41.0
7	40.05325081770478	41.0	40.0	41.0	38.0	41.0
8	40.03254375546622	41.0	40.0	41.0	38.0	41.0
9	40.01311751629104	41.0	40.0	41.0	38.0	41.0
10-14	39.95367334841107	41.0	40.0	41.0	38.0	41.0
15-19	39.815263352862246	41.0	40.0	41.0	38.0	41.0
20-24	39.63482945397061	40.4	39.2	41.0	37.0	41.0
25-29	39.39712861732121	40.0	39.0	41.0	36.2	41.0
30-34	39.30296633830722	40.0	38.8	41.0	35.8	41.0
35-39	39.14931674641633	40.0	38.2	41.0	35.0	41.0
40-44	38.65415849713379	40.0	36.8	41.0	35.0	41.0
45-49	38.03374026120055	39.0	35.4	41.0	34.6	41.0
50-54	37.372537011770746	37.4	35.0	40.2	34.0	41.0
55-59	36.68693818330818	36.2	35.0	39.2	34.0	41.0
60-64	35.95391925516687	35.2	34.8	38.0	32.8	40.0
65-69	35.540449477307234	35.0	34.8	36.8	33.0	39.0
70-74	35.244342855339276	35.0	35.0	36.0	33.0	38.2
75-79	35.016838384787704	35.0	35.0	35.4	33.0	37.0
80-84	34.917668214410064	35.0	34.6	35.0	33.0	37.0
85-89	34.72763634319239	35.0	33.8	35.0	32.4	37.2
90-94	39.340719225372574	40.6	38.8	40.6	36.6	40.6
95-99	39.60025572958889	41.0	39.0	41.0	36.8	41.0
100-104	39.48321601977837	41.0	39.0	41.0	36.0	41.0
105-109	39.328657056583594	40.0	39.0	41.0	35.8	41.0
110-114	39.13732201112127	40.0	38.2	41.0	35.0	41.0
115-119	38.87573003374656	40.0	38.0	41.0	35.0	41.0
120-124	38.57650878860842	40.0	37.4	41.0	34.2	41.0
125-129	38.118282615941034	39.6	36.6	40.6	33.8	41.0
130-134	37.88775362436487	39.0	35.4	40.8	33.8	41.0
135-139	37.54614092539269	38.4	35.0	41.0	33.6	41.0
140-144	37.099205362562586	37.0	35.0	40.0	33.8	41.0
145-149	36.4502464063417	35.8	35.0	39.0	33.0	41.0
150-154	35.81444937939756	35.0	35.0	37.2	33.0	39.6
155-159	35.26898973237103	35.0	35.0	36.2	33.0	38.4
160-164	34.853806709446964	35.0	34.8	35.4	33.0	36.8
165-169	34.57395807606298	35.0	34.0	35.0	33.0	36.0
170-174	34.412254054072726	35.0	34.0	35.0	33.0	36.0
175-178	34.10605311798421	34.75	33.75	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	4.0
29	40.0
30	462.0
31	3402.0
32	17931.0
33	69903.0
34	210085.0
35	528844.0
36	1234735.0
37	2151632.0
38	2179376.0
39	689622.0
40	10962.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.551926384659208	27.681418582699536	28.922055646337274	21.844599386303987
2	22.700510455193808	28.122915051840923	28.149405121798665	21.0271693711666
3	20.189265913662943	27.107034959424396	30.151504882556697	22.55219424435596
4	21.112191223360746	25.960860358018934	30.545065597444783	22.38188282117554
5	22.33155168825584	26.81340380631306	28.28887252203361	22.56617198339749
6	22.171357217325237	27.268398938762704	28.255041320986518	22.305202522925534
7	22.17471074745819	27.277628191859687	27.86345327088252	22.684207789799604
8	22.481164221666088	27.428663862007024	27.429058394963846	22.661113521363045
9	22.49046392707678	27.603568776041815	27.57024483165349	22.33572246522791
10-14	22.57081619991774	27.401593264984257	27.325206048359313	22.70238448673869
15-19	22.626259352720776	27.329486730940783	27.377016679867083	22.66723723647136
20-24	22.645971910098904	27.16972906435523	27.41234428805753	22.77195473748834
25-29	22.727104794575848	27.104039327045136	27.194908721277827	22.97394715710119
30-34	22.814741836655184	27.15353066838533	27.142545743630514	22.889181751328977
35-39	22.855178646636418	27.124915756645873	27.113696930209514	22.906208666508196
40-44	22.871405223531806	27.047032132877575	27.073155850803982	23.008406792786644
45-49	23.00017232635935	27.02425067271392	27.03748443532259	22.938092565604137
50-54	23.001784275297208	27.09614021363114	26.988443988790188	22.91363152228146
55-59	22.952422566214256	27.01399281583667	26.993288289881402	23.04029632806768
60-64	22.89083597165506	27.09004186135576	27.058952664358554	22.96016950263062
65-69	22.91868154412872	27.095931674782538	27.026457239179546	22.958929541909193
70-74	22.8626606823532	27.075286328770794	27.011938426368665	23.050114562507336
75-79	22.828826663213565	27.111808808201886	27.06343343151098	22.995931097073566
80-84	22.84066828810318	27.120615347416567	27.033195298463475	23.005521066016776
85-89	22.794998280259023	27.07962055511069	27.01010948430456	23.115271680325726
90-94	21.560663609546417	27.296098682046754	28.945862903592246	22.197374804814586
95-99	22.174453322263048	27.529803377276718	27.694708915827864	22.601034384632367
100-104	22.460303502635114	27.50949697682259	27.327043662157845	22.70315585838445
105-109	22.447519555323492	27.37067629248191	27.34179839251278	22.84000575968182
110-114	22.47355822770116	27.247797648846557	27.397599357474533	22.88104476597774
115-119	22.5899090630899	27.292405499624213	27.163122480126834	22.954562957159048
120-124	22.594379789951745	27.24232199430699	27.115131930393975	23.048166285347286
125-129	22.604652318260865	27.23500112270597	27.178585764274	22.98176079475916
130-134	22.74489467864703	27.249135997798824	27.01924028625268	22.986729037301473
135-139	22.862138687655104	27.17482139751914	26.95775632204615	23.005283592779605
140-144	22.823023816357583	27.23342931476663	27.07119908894794	22.872347779927843
145-149	22.797231641597325	27.293019522524702	27.060037477504885	22.84971135837309
150-154	22.71040483855388	27.325478959087857	27.098803526390274	22.86531267596799
155-159	22.656806802979908	27.424855502566096	27.167727520664197	22.750610173789802
160-164	22.602037161513618	27.53041910898215	27.191681441837734	22.675862287666494
165-169	22.496666397694057	27.557356566671494	27.250175108537817	22.695801927096632
170-174	22.414248638730434	27.69105601150088	27.348491752044435	22.546203597724254
175-178	22.372457686222145	27.76579821642881	27.312367786892416	22.54937631045663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	3.0
10	8.5
11	20.5
12	39.5
13	76.5
14	157.0
15	276.5
16	479.5
17	880.5
18	1549.0
19	2597.5
20	4230.5
21	6720.5
22	10280.5
23	15215.5
24	21441.5
25	28868.5
26	37796.0
27	48404.5
28	60349.5
29	73592.5
30	88171.5
31	103871.5
32	120061.5
33	136872.0
34	154411.0
35	172614.0
36	191381.5
37	209680.5
38	226797.0
39	242371.5
40	256120.5
41	267797.0
42	276518.0
43	281349.0
44	282168.0
45	280080.5
46	274068.0
47	263866.5
48	251549.0
49	237837.0
50	223105.5
51	208166.5
52	194221.5
53	181129.0
54	169677.0
55	159348.0
56	149743.0
57	141636.0
58	133965.0
59	126010.5
60	117466.5
61	108296.0
62	98766.0
63	88078.0
64	76730.0
65	65387.0
66	54333.5
67	44286.0
68	35063.5
69	26938.0
70	20136.0
71	14649.0
72	10382.0
73	7128.0
74	4800.0
75	3163.5
76	2009.0
77	1194.5
78	676.5
79	387.0
80	219.5
81	114.0
82	49.0
83	22.5
84	13.0
85	7.5
86	3.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1330.0
92-93	2812.0
94-95	4146.0
96-97	5366.0
98-99	6535.0
100-101	7492.0
102-103	8719.0
104-105	10064.0
106-107	11173.0
108-109	12169.0
110-111	12901.0
112-113	13825.0
114-115	14477.0
116-117	15406.0
118-119	16066.0
120-121	16810.0
122-123	17683.0
124-125	18773.0
126-127	19480.0
128-129	20172.0
130-131	21102.0
132-133	23396.0
134-135	99048.0
136-137	81727.0
138-139	77723.0
140-141	78402.0
142-143	77322.0
144-145	81328.0
146-147	82084.0
148-149	84752.0
150-151	85447.0
152-153	80694.0
154-155	82249.0
156-157	83444.0
158-159	84200.0
160-161	87518.0
162-163	87253.0
164-165	88906.0
166-167	92909.0
168-169	94804.0
170-171	95468.0
172-173	100053.0
174-175	105662.0
176-177	236760.0
178-179	4749349.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.7230446837864
#Duplication Level	Percentage of total
1	95.59629834943826
2	4.019939411679506
3	0.2950388202771933
4	0.0459470215454201
5	0.019536637940224446
6	0.010354766380892728
7	0.00417097009501886
8	0.0
9	0.0
>10	0.008714022643503915
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.636185097391165E-5	0.0	0.0	1.4090462743477912E-5	0.0	0.0
2	9.863323920434538E-5	0.0	0.0	1.4090462743477912E-5	0.0	0.0
3	9.863323920434538E-5	0.0	0.0	4.227138823043374E-5	0.0	0.0
4	9.863323920434538E-5	0.0	0.0	4.227138823043374E-5	1.4090462743477912E-5	0.0
5	9.863323920434538E-5	0.0	0.0	4.227138823043374E-5	1.4090462743477912E-5	0.0
6	9.863323920434538E-5	2.8180925486955824E-5	0.0	7.045231371738956E-5	1.4090462743477912E-5	0.0
7	9.863323920434538E-5	2.8180925486955824E-5	1.4090462743477912E-5	7.045231371738956E-5	1.4090462743477912E-5	0.0
8	9.863323920434538E-5	5.636185097391165E-5	1.4090462743477912E-5	8.454277646086748E-5	1.4090462743477912E-5	0.0
9	9.863323920434538E-5	7.045231371738956E-5	1.4090462743477912E-5	9.863323920434538E-5	1.4090462743477912E-5	0.0
10-14	9.863323920434538E-5	7.327040626608514E-5	2.8180925486955824E-5	1.1554179449651888E-4	2.5362832938260247E-5	0.0
15-19	1.0426942430173654E-4	1.1554179449651888E-4	3.945329568173815E-5	1.6626746037303933E-4	4.227138823043374E-5	0.0
20-24	1.2399607214260563E-4	1.268141646913012E-4	6.199803607130282E-5	2.395378666391245E-4	7.045231371738956E-5	0.0
25-29	1.268141646913012E-4	1.4372271998347469E-4	7.608849881478072E-5	2.846273474182538E-4	8.17246839121719E-5	0.0
30-34	1.3808653488608355E-4	1.5499509017825704E-4	9.017896155825865E-5	3.381711058434699E-4	1.268141646913012E-4	0.0
35-39	1.9726647840869076E-4	1.5499509017825704E-4	1.0426942430173654E-4	3.6071584623303455E-4	1.803579231165173E-4	1.6908555292173495E-5
40-44	2.958997176130362E-4	1.5499509017825704E-4	1.127237019478233E-4	3.860786791712948E-4	2.536283293826024E-4	5.636185097391165E-5
45-49	3.57897753684339E-4	1.6626746037303936E-4	1.1554179449651888E-4	4.114415121095551E-4	3.1844445800260077E-4	1.2399607214260563E-4
50-54	3.9735104936607714E-4	1.803579231165173E-4	1.606312752756482E-4	4.5934908543737995E-4	3.381711058434699E-4	1.916302933112996E-4
55-59	4.198957897556418E-4	1.944483858599952E-4	1.8881220076260403E-4	5.044385662165092E-4	3.7762440152520806E-4	2.5081023683390683E-4
60-64	4.734395481808579E-4	2.1135694115216865E-4	2.3108358899303778E-4	5.46709954446943E-4	4.170776972069462E-4	2.8180925486955824E-4
65-69	5.298013991547696E-4	2.1417503370086427E-4	2.5644642193129797E-4	5.748908799338987E-4	4.6498527053477113E-4	2.9308162506434056E-4
70-74	7.580668955991116E-4	2.536283293826024E-4	2.7899116232086267E-4	6.227984532617238E-4	4.875300109243357E-4	2.9871781016173175E-4
75-79	0.004266592118725112	2.705368846747759E-4	2.846273474182538E-4	6.650698414921574E-4	5.016204736678137E-4	3.325349207460787E-4
80-84	0.005771453539728554	2.9308162506434056E-4	3.325349207460787E-4	7.017050446252E-4	5.974356203234635E-4	3.522615685869478E-4
85-89	0.0058306334832511604	3.0999018035651407E-4	3.4380729094086115E-4	7.637030806965028E-4	8.172468391217189E-4	3.522615685869478E-4
90-94	0.005878541056578985	3.0999018035651407E-4	3.522615685869478E-4	7.918840061834586E-4	8.369734869625881E-4	3.522615685869478E-4
95-99	0.005889813426773767	3.212625505512964E-4	3.6635203133042574E-4	8.70790597546935E-4	8.454277646086746E-4	3.522615685869478E-4
100-104	0.005889813426773767	3.494434760382523E-4	3.7198821642781693E-4	9.637876516538892E-4	8.736086900956305E-4	3.522615685869478E-4
105-109	0.005895449611871159	3.888967717199904E-4	3.832605866225992E-4	0.001006059039884323	8.961534304851952E-4	3.522615685869478E-4
110-114	0.005912358167163331	4.227138823043374E-4	3.9735104936607714E-4	0.001053966613212148	9.215162634234554E-4	3.522615685869478E-4
115-119	0.005932084815004201	4.508948077912932E-4	4.170776972069462E-4	0.0010765113536017124	9.468790963617156E-4	3.522615685869478E-4
120-124	0.005940539092650288	4.6498527053477113E-4	4.3680434504781535E-4	0.0011244189269295373	9.806962069460626E-4	3.522615685869478E-4
125-129	0.006025081869111155	4.7907573327824897E-4	4.3680434504781535E-4	0.0011469636673191019	9.976047622382363E-4	3.6635203133042574E-4
130-134	0.006070171349890285	5.128928438625959E-4	4.424405301452065E-4	0.0011723265002573622	0.0010201495026278006	3.8044249407390363E-4
135-139	0.006103988460474632	5.354375842521607E-4	4.7062145563216227E-4	0.0011976893331956226	0.0010370580579199744	3.8044249407390363E-4
140-144	0.00614907794125376	5.354375842521607E-4	4.847119183756401E-4	0.0012258702586825782	0.0010426942430173656	3.8044249407390363E-4
145-149	0.006171622681643326	5.354375842521607E-4	5.241652140573784E-4	0.001273777832010403	0.0010708751685043214	3.888967717199904E-4
150-154	0.006242074995360715	5.551642320930297E-4	5.438918618982473E-4	0.0013188673127895324	0.0011300551120269286	4.142596046582506E-4
155-159	0.006343526327113757	5.889813426773767E-4	5.551642320930297E-4	0.001383683441409531	0.0011469636673191019	4.3680434504781535E-4
160-164	0.006419614825928536	6.002537128721591E-4	5.720727873852032E-4	0.0014203186445425737	0.0011582360375138843	4.3680434504781535E-4
165-167	0.0064628255783418685	6.058898979695503E-4	5.777089724825944E-4	0.001423136737091269	0.00119299251228113	4.4619798687680056E-4
>>END_MODULE
