##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478983
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7077539
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23209649568869	39.0	39.0	39.0	37.0	39.0
2	39.943069900427254	41.0	40.0	41.0	38.0	41.0
3	39.984034280842536	41.0	40.0	41.0	38.0	41.0
4	39.998836318669525	41.0	40.0	41.0	38.0	41.0
5	40.01420041627464	41.0	40.0	41.0	38.0	41.0
6	39.99890470402212	41.0	40.0	41.0	38.0	41.0
7	40.02258694724254	41.0	40.0	41.0	38.0	41.0
8	40.01477448587708	41.0	40.0	41.0	38.0	41.0
9	39.9987444788365	41.0	40.0	41.0	38.0	41.0
10-14	39.936309753997826	41.0	40.0	41.0	38.0	41.0
15-19	39.809773114637736	41.0	40.0	41.0	37.8	41.0
20-24	39.65008240858863	40.6	39.2	41.0	37.0	41.0
25-29	39.43818180302504	40.0	39.0	41.0	36.4	41.0
30-34	39.310007532279236	40.0	38.6	41.0	35.6	41.0
35-39	38.91308111477733	40.0	37.8	41.0	35.0	41.0
40-44	38.6264676747101	40.0	36.8	41.0	35.0	41.0
45-49	38.039657146361186	39.0	35.4	41.0	34.6	41.0
50-54	37.37828355308251	37.4	35.0	40.2	34.0	41.0
55-59	36.70258868230893	36.2	35.0	39.2	34.0	41.0
60-64	35.960984884717696	35.2	34.8	38.0	32.8	40.0
65-69	35.54409548290727	35.0	34.8	36.8	33.0	39.0
70-74	35.255923139385025	35.0	35.0	36.0	33.0	38.2
75-79	35.0315197980541	35.0	35.0	35.4	33.0	37.0
80-84	34.93556559702462	35.0	35.0	35.0	33.0	37.0
85-89	34.75977059257462	35.0	34.0	35.0	32.6	37.2
90-94	39.27865085726249	40.6	38.8	40.6	36.4	40.6
95-99	39.54980231479636	41.0	39.0	41.0	36.2	41.0
100-104	39.44391534136261	41.0	39.0	41.0	36.0	41.0
105-109	39.29287990016187	40.0	39.0	41.0	35.4	41.0
110-114	39.11050952772764	40.0	38.0	41.0	35.0	41.0
115-119	38.85079421771295	40.0	38.0	41.0	35.0	41.0
120-124	38.56025781122414	40.0	37.4	41.0	34.0	41.0
125-129	38.065878107704286	39.4	36.2	40.6	33.6	41.0
130-134	37.83556817846166	39.0	35.2	40.8	33.6	41.0
135-139	37.455624616041774	38.2	35.0	40.8	33.4	41.0
140-144	37.07870396050436	37.0	35.0	40.0	33.8	41.0
145-149	36.44337624950405	35.8	35.0	39.0	33.0	41.0
150-154	35.81622606347692	35.0	35.0	37.2	33.0	39.8
155-159	35.27466771135227	35.0	35.0	36.2	33.0	38.4
160-164	34.863041931853864	35.0	35.0	35.4	33.0	36.8
165-169	34.59444562093311	35.0	34.2	35.0	33.0	36.0
170-174	34.44171419876629	35.0	34.0	35.0	33.0	36.0
175-178	34.15657426251393	35.0	33.75	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	9.0
29	70.0
30	703.0
31	4630.0
32	22248.0
33	80167.0
34	223889.0
35	535272.0
36	1219900.0
37	2120585.0
38	2156136.0
39	701531.0
40	12399.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.528811074018808	27.648819738047365	28.955813595658036	21.86655559227579
2	22.693947712615923	28.13370579801821	28.123801225256408	21.048545264109457
3	20.199394167944533	27.06882999867609	30.18403713494196	22.54773869843741
4	21.102645990364728	25.942294348360356	30.583144790865862	22.37191487040905
5	22.367195715911986	26.773586129302856	28.315845380717793	22.543372774067368
6	22.133555180692046	27.30597457675613	28.239830257381836	22.320639985169986
7	22.161220164240707	27.28503509482604	27.885356760308916	22.66838798062434
8	22.51531217277644	27.414274933702238	27.449753367660705	22.620659525860614
9	22.475481943653016	27.624418600872424	27.581338654580357	22.318760800894207
10-14	22.555577581416365	27.405486568141836	27.323361976528847	22.715573873912952
15-19	22.610935806923848	27.32623585684233	27.38183145299517	22.680996883238652
20-24	22.651472496301327	27.153729566166994	27.407936572302887	22.786861365228788
25-29	22.73631272113089	27.07890129605785	27.20343045796003	22.981355524851224
30-34	22.77750500562413	27.14166887670983	27.167847467883966	22.91297864978208
35-39	22.81769976823865	27.139868815982503	27.113125056605124	22.92930635917372
40-44	22.871515084551284	27.04254685138436	27.059778829901184	23.026159234163174
45-49	22.983938343540036	27.0283780845291	27.025235749319076	22.962447822611786
50-54	22.997089807629457	27.081289131716545	26.983461341576497	22.938159719077493
55-59	22.949338746137606	27.016023507606246	26.977004294854467	23.057633451401678
60-64	22.89955307911408	27.089772306447195	27.04572592252759	22.96494869191113
65-69	22.925070423490425	27.109612536221984	26.98437691406575	22.980940126221842
70-74	22.84957808074247	27.07720296560711	26.995134890814448	23.078084062835966
75-79	22.841804192106892	27.10918866006955	27.03835047747529	23.01065667034827
80-84	22.843601426993196	27.1276103176542	27.005477468933766	23.02331078641884
85-89	22.797161555732863	27.084321259126938	26.965011425581686	23.153505759558513
90-94	21.58381290066387	27.290417047272108	28.94384707240655	22.18192297965747
95-99	22.18076914476539	27.53787004976313	27.67284273679378	22.608518068677704
100-104	22.47624669299735	27.494967396447983	27.317722696343193	22.711063214211475
105-109	22.461926541250715	27.383280631580252	27.33395017293288	22.820842654236152
110-114	22.495039815241327	27.23565254594968	27.400348734400737	22.868958904408256
115-119	22.61394176566887	27.293411543833727	27.154168954368007	22.938477736129396
120-124	22.63008904179593	27.237027465579487	27.10366492837647	23.029218564248115
125-129	22.62522799983288	27.25578393821665	27.16129380431437	22.957694257636103
130-134	22.789203869476456	27.233105428058856	27.001621300019686	22.976069402445003
135-139	22.895142596988315	27.16940568099014	26.91790572758598	23.017545994435572
140-144	22.834843624180834	27.239441530176034	27.046351537259493	22.87936330838364
145-149	22.816824479289195	27.30557872974727	27.024126225622215	22.85347056534132
150-154	22.742348042861924	27.332445322585443	27.05062394825477	22.874582686297867
155-159	22.671871790397642	27.404996460464325	27.147034161765067	22.776097587372966
160-164	22.64000891511153	27.51035112430657	27.156113126110537	22.693526834471363
165-169	22.520286291040655	27.554217177835778	27.212638410652396	22.712858120471168
170-174	22.455162842341895	27.66965412059769	27.306758335859104	22.56842470120131
175-178	22.39457112515522	27.739419350629134	27.27684908654767	22.58916043766797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	2.5
9	6.5
10	7.5
11	15.0
12	37.5
13	69.5
14	148.0
15	276.0
16	493.0
17	868.0
18	1531.0
19	2656.5
20	4299.0
21	6720.5
22	10309.0
23	15170.0
24	21249.0
25	28845.5
26	37946.5
27	48486.0
28	60610.5
29	73486.5
30	87708.5
31	103173.0
32	119625.0
33	136773.5
34	154147.0
35	172564.0
36	190849.0
37	208520.5
38	225603.0
39	241515.5
40	255279.5
41	266782.0
42	275648.0
43	280598.5
44	281484.5
45	278038.0
46	271537.5
47	262617.0
48	250645.0
49	236730.0
50	222476.0
51	207874.5
52	193703.5
53	180707.0
54	168857.0
55	158833.0
56	149628.0
57	141520.5
58	133696.0
59	125494.0
60	117633.5
61	108869.5
62	98632.0
63	87910.0
64	77019.5
65	65757.0
66	54738.0
67	44382.5
68	35196.5
69	27287.0
70	20371.0
71	14704.5
72	10427.0
73	7169.0
74	4774.5
75	3082.0
76	1959.5
77	1201.5
78	670.5
79	373.5
80	210.0
81	113.5
82	57.0
83	29.5
84	16.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1346.0
92-93	2936.0
94-95	4330.0
96-97	5748.0
98-99	7033.0
100-101	8358.0
102-103	9307.0
104-105	10366.0
106-107	11816.0
108-109	12716.0
110-111	13302.0
112-113	14405.0
114-115	14991.0
116-117	16032.0
118-119	16767.0
120-121	18626.0
122-123	18564.0
124-125	20287.0
126-127	21115.0
128-129	21430.0
130-131	22248.0
132-133	26022.0
134-135	101581.0
136-137	88616.0
138-139	86941.0
140-141	83256.0
142-143	84133.0
144-145	81538.0
146-147	85274.0
148-149	89607.0
150-151	81975.0
152-153	78194.0
154-155	81559.0
156-157	83562.0
158-159	89127.0
160-161	87042.0
162-163	86845.0
164-165	89495.0
166-167	88221.0
168-169	89114.0
170-171	94238.0
172-173	99197.0
174-175	102965.0
176-177	234033.0
178-179	4693281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.8175286788282
#Duplication Level	Percentage of total
1	95.76614746774602
2	3.886189896626729
3	0.2798463178621667
4	0.045950420730524216
5	0.008226708173964243
6	0.006213540668236244
7	0.0031286256662707485
8	0.0
9	0.0
>10	0.004297022526082965
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.651682032412679E-5	0.0	0.0	0.0	1.4129205081031697E-5	0.0
2	1.2716284572928527E-4	1.4129205081031697E-5	1.4129205081031697E-5	0.0	1.4129205081031697E-5	0.0
3	1.4129205081031697E-4	4.238761524309509E-5	1.4129205081031697E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
4	1.4129205081031697E-4	4.238761524309509E-5	1.4129205081031697E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
5	1.5542125589134868E-4	4.238761524309509E-5	2.8258410162063394E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
6	1.6955046097238036E-4	5.651682032412679E-5	4.238761524309509E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
7	1.9780887113444377E-4	5.651682032412679E-5	4.238761524309509E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
8	2.1193807621547548E-4	5.651682032412679E-5	4.238761524309509E-5	1.4129205081031697E-5	1.4129205081031697E-5	0.0
9	2.1193807621547548E-4	5.651682032412679E-5	4.238761524309509E-5	2.8258410162063394E-5	2.8258410162063394E-5	0.0
10-14	2.2606728129650715E-4	6.216850235653947E-5	5.651682032412679E-5	6.499434337274581E-5	2.8258410162063394E-5	0.0
15-19	2.2889312231271348E-4	8.477523048619018E-5	9.325275353480918E-5	9.325275353480921E-5	3.1084251178269735E-5	0.0
20-24	2.5997737349098325E-4	9.607859455101554E-5	1.0455611759963457E-4	1.3281452776169796E-4	4.5213456259301433E-5	0.0
25-29	2.9671330670166563E-4	9.890443556722188E-5	1.2433700471307894E-4	1.58247096907555E-4	7.629770743757116E-5	0.0
30-34	3.2497171686372904E-4	1.1303364064825358E-4	1.3281452776169796E-4	2.147639172316818E-4	1.0455611759963457E-4	1.1303364064825358E-5
35-39	3.899660602364748E-4	1.3564036877790431E-4	2.0628639418306276E-4	2.5432569145857054E-4	1.5542125589134868E-4	5.086513829171411E-5
40-44	4.182244703985382E-4	1.4129205081031697E-4	2.486740094261579E-4	2.9671330670166563E-4	2.2606728129650715E-4	7.064602540515849E-5
45-49	4.606120856416334E-4	1.4976957385893602E-4	2.5432569145857054E-4	3.560559680419988E-4	2.5997737349098325E-4	8.477523048619018E-5
50-54	4.973480188523158E-4	1.7520214300479302E-4	2.769324195882213E-4	4.1257278836612563E-4	2.882357836530466E-4	8.477523048619018E-5
55-59	5.199547469819665E-4	2.1193807621547548E-4	3.08016670766491E-4	4.351795164957763E-4	3.391009219447607E-4	8.477523048619018E-5
60-64	5.877749313709186E-4	2.4019648637753883E-4	3.1084251178269736E-4	5.312581110467919E-4	3.786626961716495E-4	8.477523048619018E-5
65-69	6.16033341532982E-4	2.5432569145857054E-4	3.1649419381511E-4	6.047299774681566E-4	4.210503114147446E-4	9.607859455101554E-5
70-74	8.675331919753462E-4	2.5432569145857054E-4	3.2497171686372904E-4	6.782018438895215E-4	5.001738598685221E-4	1.0173027658342822E-4
75-79	0.004077688586385748	2.5432569145857054E-4	3.306233988961417E-4	7.488478692946799E-4	5.425614751116171E-4	1.1868532268066625E-4
80-84	0.005343665361646188	2.6280321450718956E-4	3.391009219447607E-4	8.534039868943145E-4	6.16033341532982E-4	1.2716284572928527E-4
85-89	0.005371923771808252	2.8258410162063395E-4	3.5323012702579245E-4	9.297016943318857E-4	8.477523048619019E-4	1.2716284572928527E-4
90-94	0.0054001821819703155	3.1649419381511E-4	3.588818090582051E-4	9.353533763642984E-4	8.477523048619019E-4	1.2716284572928527E-4
95-99	0.0054256147511161715	3.391009219447607E-4	3.6735933210682413E-4	9.664376275425682E-4	8.73184874007759E-4	1.2716284572928527E-4
100-104	0.005448221479245822	3.5040428600958604E-4	3.9279190125268124E-4	0.0010201286068504884	8.760107150239651E-4	1.2716284572928527E-4
105-109	0.005482131571440299	4.0409526531750656E-4	4.1539862938233193E-4	0.0010766454271746155	8.901399201049969E-4	1.2716284572928527E-4
110-114	0.005530170868715807	4.323536754795699E-4	4.436570395443953E-4	0.0011190330424177106	8.901399201049969E-4	1.2716284572928527E-4
115-119	0.005575384324975108	4.49308721576808E-4	4.521345625930143E-4	0.0011444656115635675	9.014432841698223E-4	1.2716284572928527E-4
120-124	0.0055979910531047585	4.7756713173887136E-4	4.6343792665783974E-4	0.0011868532268066625	9.042691251860286E-4	1.2716284572928527E-4
125-129	0.00564320450936406	4.8039297275507766E-4	4.747412907226651E-4	0.0012546734111956149	9.410050583967113E-4	1.2716284572928527E-4
130-134	0.005702547170704393	4.8039297275507766E-4	4.945221778361094E-4	0.0012631509342442338	9.466567404291238E-4	1.4129205081031697E-4
135-139	0.00575906399102852	4.888704958036967E-4	5.114772239333474E-4	0.0012998868674549162	9.664376275425682E-4	1.5542125589134868E-4
140-144	0.005849490903547122	5.029997008847284E-4	5.284322700305856E-4	0.0013394486416818047	9.749151505911871E-4	1.5542125589134868E-4
145-149	0.005951221180130551	5.199547469819665E-4	5.369097930792045E-4	0.0013846620979411064	9.862185146560123E-4	1.7520214300479302E-4
150-154	0.0059907829543574395	5.538648391764426E-4	5.369097930792045E-4	0.0014750890104597092	0.0010031735607532505	1.9215718910203108E-4
155-159	0.006021867205535709	5.792974083222996E-4	5.510389981602363E-4	0.001514650784686598	0.0010201286068504884	1.9780887113444377E-4
160-164	0.006086861548908457	6.047299774681567E-4	5.906007723871249E-4	0.0015909484921241691	0.0010455611759963457	1.9780887113444377E-4
165-167	0.006136784740194767	6.216850235653947E-4	6.358142286464264E-4	0.0016342780543726662	0.0010596903810773774	1.978088711344438E-4
>>END_MODULE
