##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478986
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7920493
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.281611384543865	39.0	39.0	39.0	37.0	39.0
2	40.00579332624876	41.0	40.0	41.0	38.0	41.0
3	40.029753577207885	41.0	40.0	41.0	38.0	41.0
4	40.046198386893344	41.0	40.0	41.0	38.0	41.0
5	40.05405446352898	41.0	40.0	41.0	38.0	41.0
6	40.04152632923228	41.0	40.0	41.0	38.0	41.0
7	40.061691235633944	41.0	40.0	41.0	38.0	41.0
8	40.044072761632385	41.0	40.0	41.0	38.0	41.0
9	40.02437297779318	41.0	40.0	41.0	38.0	41.0
10-14	39.958123566298205	41.0	40.0	41.0	38.0	41.0
15-19	39.834661781785556	41.0	40.0	41.0	38.0	41.0
20-24	39.65209006560577	40.6	39.4	41.0	37.0	41.0
25-29	39.41794451431243	40.0	39.0	41.0	36.2	41.0
30-34	39.33196569960986	40.0	38.8	41.0	35.8	41.0
35-39	39.17851105985448	40.0	38.2	41.0	35.0	41.0
40-44	38.70362938266595	40.0	36.8	41.0	35.0	41.0
45-49	38.10457621766726	39.0	35.4	41.0	34.8	41.0
50-54	37.44949164149252	37.6	35.0	40.2	34.0	41.0
55-59	36.76490844698682	36.4	35.0	39.2	34.0	41.0
60-64	36.01365074118492	35.4	34.8	38.0	33.6	40.0
65-69	35.59127771465741	35.0	35.0	36.8	33.0	39.0
70-74	35.301880905645646	35.0	35.0	36.0	33.0	38.0
75-79	35.07241402776317	35.0	35.0	35.4	33.0	37.0
80-84	34.971400391364526	35.0	35.0	35.0	33.0	37.0
85-89	34.78548428740484	35.0	34.6	35.0	32.6	37.0
90-94	39.51747920175836	40.6	39.8	40.6	37.6	40.6
95-99	39.783348047076075	41.0	40.0	41.0	37.0	41.0
100-104	39.675688001471464	41.0	39.6	41.0	37.0	41.0
105-109	39.53442767281464	41.0	39.0	41.0	36.0	41.0
110-114	39.345566487534896	40.0	38.8	41.0	35.6	41.0
115-119	39.088088405465705	40.0	38.0	41.0	35.0	41.0
120-124	38.79406371504953	40.0	38.0	41.0	35.0	41.0
125-129	38.34505233460314	39.6	36.8	40.6	34.4	41.0
130-134	38.1296902060697	39.0	35.8	41.0	34.0	41.0
135-139	37.78644180720896	39.0	35.0	41.0	34.0	41.0
140-144	37.3199406732185	37.2	35.0	40.4	34.0	41.0
145-149	36.64591445509109	36.2	35.0	39.0	34.0	41.0
150-154	35.9972195279133	35.0	35.0	37.4	34.0	39.8
155-159	35.44627147732207	35.0	35.0	36.4	33.2	38.6
160-164	35.034027535043734	35.0	35.0	35.8	33.0	37.0
165-169	34.75855325880521	35.0	35.0	35.0	33.0	36.0
170-174	34.60157318052885	35.0	35.0	35.0	33.0	36.0
175-178	34.30089852692234	35.0	34.25	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	50.0
30	609.0
31	4225.0
32	21152.0
33	77011.0
34	215203.0
35	520116.0
36	1235807.0
37	2285789.0
38	2643742.0
39	890868.0
40	25920.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.168721063196443	28.43910094990299	27.871232257891016	21.520945729009544
2	22.542687683708575	29.144789345814708	27.961504416454886	20.351018554021827
3	20.424448326638252	28.167337563457224	29.675526510786636	21.732687599117885
4	21.136108573039582	27.204102067888957	30.000998675208727	21.658790683862733
5	22.020523217431037	27.956782488160776	28.48097965619059	21.541714638217595
6	21.906805548594008	27.947906778025057	28.473720007075315	21.67156766630562
7	22.090089594170465	28.0491883522907	28.011008910682705	21.849713142856135
8	22.19828992967988	28.359181682251343	27.814442863594476	21.628085524474297
9	22.107007732978236	28.291471250590085	27.986944752050157	21.614576264381522
10-14	22.149979805549982	28.31695451280621	27.79851961235241	21.734546069291394
15-19	22.04974362075694	28.31234368870725	27.95125252935644	21.68666016117936
20-24	21.974932620987104	28.248297170390785	28.07179300581416	21.704977202807957
25-29	21.999855312036765	28.283252065243918	27.8870090536031	21.829883569116216
30-34	21.994186472988485	28.29581693967787	27.971655299739552	21.738341287594093
35-39	21.950121034132597	28.359545295980944	27.97257695954027	21.717756710346187
40-44	21.959734072108894	28.35859838522678	27.961322609590084	21.720344933074244
45-49	22.04790282625084	28.260687813245966	27.923324974846892	21.768084385656298
50-54	22.014361984790593	28.3745645630897	27.952611030651752	21.658462421467956
55-59	22.016023497527236	28.302558944247536	27.91646429079604	21.76495326742919
60-64	21.928321886024012	28.323188973211643	28.00299678315479	21.745492357609557
65-69	21.933361976331526	28.334251415915652	28.011087188638385	21.72129941911444
70-74	21.913110711669084	28.354719838777715	27.978273574637335	21.753895874915866
75-79	21.891718103910957	28.332526775795397	28.01968766338156	21.75606745691209
80-84	21.87278746411366	28.41076685504299	27.97971287898367	21.73673280185968
85-89	21.90946699908705	28.35663891123949	27.914126052507086	21.819768037166373
90-94	21.419929241220643	28.127291651702578	28.837177726653223	21.615601380423552
95-99	21.784321586727035	28.120152636962963	28.185438528389312	21.91008724792069
100-104	21.83090773461213	28.24174713319816	28.028917977290707	21.898427154899004
105-109	21.700846780613162	28.21811641146529	28.176424085491895	21.90461272242965
110-114	21.690833236120728	28.041542883040982	28.267440514676913	22.00018336616138
115-119	21.675432085786134	28.182611134318915	28.153422778620417	21.988534001274534
120-124	21.636321555513845	28.160593711624564	28.183141831116025	22.01994290174557
125-129	21.599683577380247	28.16282503983642	28.237367403906877	22.00012397887646
130-134	21.673052539656624	28.210139494479673	28.184740185816047	21.932067780047653
135-139	21.724347167746636	28.173485260576214	28.143919994316512	21.958247577360634
140-144	21.658296495341702	28.2274020317726	28.268216805731782	21.846084667153914
145-149	21.59205046892971	28.30831465884551	28.289813566537347	21.809821305687432
150-154	21.523386192647937	28.354754369483036	28.349846855518297	21.77201258235073
155-159	21.446610595535464	28.372909494972742	28.438409936701436	21.74206997279036
160-164	21.38051589111473	28.53312180263522	28.477825019808336	21.608537286441717
165-169	21.305217849727946	28.57549098178965	28.504799341753262	21.614491826729147
170-174	21.239202876210804	28.668238816834517	28.588005042693837	21.504553264260846
175-178	21.17945347991464	28.79281356971265	28.584594704420972	21.443138245951733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	3.0
7	14.5
8	38.0
9	68.5
10	140.0
11	262.0
12	455.0
13	817.0
14	1341.5
15	2050.0
16	3125.5
17	4717.0
18	6992.5
19	10328.0
20	14997.0
21	20908.0
22	28535.5
23	37523.5
24	47675.0
25	59057.5
26	71300.5
27	84932.0
28	99422.5
29	114506.5
30	130887.5
31	148006.0
32	165323.0
33	182942.5
34	200599.5
35	218834.0
36	237119.0
37	254333.0
38	270251.0
39	284705.0
40	297283.0
41	306397.5
42	312650.5
43	315602.0
44	313452.5
45	306779.5
46	295228.5
47	280374.0
48	263434.0
49	244301.0
50	224994.0
51	206114.0
52	187432.5
53	170660.0
54	156400.0
55	144087.0
56	133392.5
57	124104.5
58	116671.5
59	109493.5
60	101804.5
61	94070.0
62	86168.0
63	77801.5
64	68967.0
65	60010.0
66	50987.0
67	42075.5
68	33827.5
69	26638.5
70	20373.0
71	15191.5
72	11011.0
73	7665.5
74	5167.0
75	3365.0
76	2113.0
77	1292.5
78	755.5
79	451.5
80	237.0
81	123.5
82	68.5
83	32.0
84	15.5
85	5.5
86	3.5
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1215.0
92-93	2495.0
94-95	3605.0
96-97	4596.0
98-99	5567.0
100-101	6663.0
102-103	7651.0
104-105	8526.0
106-107	9432.0
108-109	10569.0
110-111	11260.0
112-113	11896.0
114-115	13787.0
116-117	13216.0
118-119	14037.0
120-121	15267.0
122-123	15099.0
124-125	15910.0
126-127	18755.0
128-129	17270.0
130-131	19813.0
132-133	19526.0
134-135	91232.0
136-137	78900.0
138-139	73547.0
140-141	69419.0
142-143	69392.0
144-145	74488.0
146-147	74334.0
148-149	76292.0
150-151	76620.0
152-153	81732.0
154-155	72907.0
156-157	71742.0
158-159	72029.0
160-161	74615.0
162-163	76626.0
164-165	80199.0
166-167	83901.0
168-169	94233.0
170-171	87671.0
172-173	89230.0
174-175	100413.0
176-177	232304.0
178-179	5772512.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.75555474621858
#Duplication Level	Percentage of total
1	90.32354008216333
2	7.731534820065359
3	1.270964336360015
4	0.3615048189273913
5	0.1516643854973291
6	0.06290454567574617
7	0.03424448033187378
8	0.021928782764535144
9	0.01260924876265321
>10	0.029104499451758154
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.525095344443837E-5	1.2625476722219186E-5	0.0	1.2625476722219186E-5	2.525095344443837E-5	0.0
2	6.944012197220552E-4	2.525095344443837E-5	0.0	1.2625476722219186E-5	2.525095344443837E-5	0.0
3	7.070266964442744E-4	2.525095344443837E-5	0.0	2.525095344443837E-5	3.787643016665756E-5	0.0
4	7.070266964442744E-4	3.787643016665756E-5	1.2625476722219186E-5	2.525095344443837E-5	3.787643016665756E-5	0.0
5	7.196521731664935E-4	6.312738361109593E-5	1.2625476722219186E-5	2.525095344443837E-5	6.312738361109593E-5	0.0
6	7.449031266109319E-4	6.312738361109593E-5	2.525095344443837E-5	2.525095344443837E-5	7.575286033331512E-5	0.0
7	7.575286033331511E-4	6.312738361109593E-5	3.787643016665756E-5	3.787643016665756E-5	7.575286033331512E-5	0.0
8	7.575286033331511E-4	6.312738361109593E-5	3.787643016665756E-5	5.050190688887674E-5	1.0100381377775348E-4	0.0
9	7.575286033331511E-4	6.312738361109593E-5	3.787643016665756E-5	6.312738361109593E-5	1.1362929049997266E-4	0.0
10-14	7.625787940220387E-4	9.59536230888658E-5	4.79768115444329E-5	8.080305102220277E-5	1.8685705548884395E-4	0.0
15-19	8.105556055664716E-4	1.23729671877748E-4	7.322776498887128E-5	9.847871843330963E-5	2.449342484110522E-4	0.0
20-24	8.20655986944247E-4	1.5403081601107405E-4	1.0352890912219731E-4	1.2625476722219185E-4	4.469418759665592E-4	0.0
25-29	8.20655986944247E-4	1.7423157876662477E-4	1.2372967187774798E-4	1.4645552997774255E-4	6.641000755887291E-4	0.0
30-34	9.443856588219948E-4	2.0705781824439462E-4	1.3888024394441103E-4	1.7928176945551243E-4	0.0010277138051886417	0.0
35-39	0.0013383005325552336	2.6008482047771523E-4	1.3888024394441103E-4	2.1210800893328228E-4	0.001456980013744094	0.0
40-44	0.001719589929566253	2.878608692665974E-4	1.641311973888494E-4	2.32308771688833E-4	0.002012500989521738	7.5752860333315115E-6
45-49	0.002151381233466149	3.333125854665865E-4	1.9443234152217542E-4	2.676601065110467E-4	0.0023180375261994427	3.0301144133326046E-5
50-54	0.0025579215839216067	3.686639202888002E-4	2.2220839031105764E-4	3.0553653667770425E-4	0.0027220527813104564	3.787643016665756E-5
55-59	0.002944261171621514	3.8633958769990705E-4	2.3483386703327687E-4	3.535133482221372E-4	0.0030629406528103744	3.787643016665756E-5
60-64	0.0032144463734770047	4.3431639924434E-4	2.5503462978882755E-4	3.6866392028880017E-4	0.0035073574334324896	3.787643016665756E-5
65-69	0.0033987783336214042	4.5956735268877833E-4	2.7271029719993443E-4	3.9391487373323863E-4	0.003848245304932407	4.2926620855545227E-5
70-74	0.006052653540631878	5.024939735443235E-4	2.979612506443728E-4	4.747179247554413E-4	0.004173982604365663	6.312738361109593E-5
75-79	0.05447135677034245	5.050190688887674E-4	3.282623947776988E-4	5.10069259577655E-4	0.004567897478098901	6.565247895553977E-5
80-84	0.07523016559701523	5.226947362998743E-4	3.7623920632213174E-4	5.353202130220934E-4	0.004822932107887729	7.575286033331512E-5
85-89	0.07537409603164853	5.858221199109701E-4	4.1664073183323303E-4	5.555209757776441E-4	0.005173920360765423	7.575286033331512E-5
90-94	0.0754927755128374	6.21173454733184E-4	4.3684149458878376E-4	5.807719292220825E-4	0.005221897172309854	7.575286033331512E-5
95-99	0.0756139800893707	6.590498848998415E-4	4.6714263872210985E-4	6.36324026799847E-4	0.005272399079198732	7.575286033331512E-5
100-104	0.0757402348565929	6.868259336887236E-4	4.747179247554413E-4	6.691502662776168E-4	0.005365827606943154	7.575286033331512E-5
105-109	0.07590436605398174	7.095517917887182E-4	4.7976811544432906E-4	6.944012197220552E-4	0.00541632951383203	7.575286033331512E-5
110-114	0.07607607253740392	7.575286033331512E-4	5.050190688887674E-4	7.27227459199825E-4	0.0055324838996764466	7.575286033331512E-5
115-119	0.07631343149978165	8.080305102220279E-4	5.403704037109811E-4	7.600536986775948E-4	0.005681464524998634	7.575286033331512E-5
120-124	0.0767199718502371	8.307563683220224E-4	5.782468338776386E-4	8.105556055664716E-4	0.0059769006802985625	7.575286033331512E-5
125-129	0.07729569358877028	8.332814636664661E-4	5.933974059443017E-4	8.711578938331236E-4	0.006504645607287324	7.827795567775894E-5
130-134	0.07811887467105899	8.534822264220169E-4	6.085479780109647E-4	9.166096100331129E-4	0.006898560481020563	1.08579099811085E-4
135-139	0.07906831052056987	8.938837519331182E-4	6.312738361109592E-4	9.645864215775457E-4	0.007403579549909329	1.2625476722219185E-4
140-144	0.07985866536338079	9.115594193442251E-4	6.464244081776223E-4	0.0010302389005330855	0.007762143088820355	1.2625476722219185E-4
145-149	0.0807121475898028	9.317601820997758E-4	7.12076887133162E-4	0.0010883160934552938	0.008166158343931368	1.2625476722219185E-4
150-154	0.08141664919090263	9.469107541664387E-4	7.600536986775948E-4	0.0011337678096552829	0.008504521120086844	1.2625476722219185E-4
155-159	0.08240396147058018	9.620613262331018E-4	7.903548428109209E-4	0.0011842697165441595	0.008893385803131194	1.2625476722219185E-4
160-164	0.0834594513245577	0.0010049879470886472	7.954050334998085E-4	0.0012120457653330419	0.009294875962897764	1.2625476722219185E-4
165-167	0.08423297219840566	0.0010310805989812335	7.954050334998086E-4	0.0012120457653330419	0.009536443417516223	1.2625476722219185E-4
>>END_MODULE
