##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478987
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6889064
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.174148331326286	39.0	39.0	39.0	37.0	39.0
2	39.92771543420122	41.0	40.0	41.0	38.0	41.0
3	39.987786149177886	41.0	40.0	41.0	38.0	41.0
4	40.01009063059946	41.0	40.0	41.0	38.0	41.0
5	40.02746904369012	41.0	40.0	41.0	38.0	41.0
6	40.01519553309419	41.0	40.0	41.0	38.0	41.0
7	40.03685522445429	41.0	40.0	41.0	38.0	41.0
8	40.018870923539104	41.0	40.0	41.0	38.0	41.0
9	39.995576757597256	41.0	40.0	41.0	38.0	41.0
10-14	39.93305084696557	41.0	40.0	41.0	38.0	41.0
15-19	39.80187944254836	41.0	40.0	41.0	37.8	41.0
20-24	39.61710241042905	40.4	39.2	41.0	37.0	41.0
25-29	39.38025183101797	40.0	38.8	41.0	36.0	41.0
30-34	39.28066004322212	40.0	38.8	41.0	35.2	41.0
35-39	39.12107148373131	40.0	38.0	41.0	35.0	41.0
40-44	38.642961627297986	40.0	36.8	41.0	35.0	41.0
45-49	38.053849260218804	39.0	35.4	41.0	34.6	41.0
50-54	37.41026798415576	37.4	35.0	40.2	34.0	41.0
55-59	36.743376400625685	36.4	35.0	39.2	34.0	41.0
60-64	36.023379344421826	35.4	34.8	38.0	33.4	40.0
65-69	35.62288757950282	35.0	35.0	36.8	33.0	39.0
70-74	35.334009351633256	35.0	35.0	36.0	33.0	38.6
75-79	35.11366351074689	35.0	35.0	35.6	33.0	37.0
80-84	35.00605916856049	35.0	35.0	35.0	33.0	37.0
85-89	34.82472353283407	35.0	34.6	35.0	32.6	37.2
90-94	39.446785029079706	40.6	39.8	40.6	36.8	40.6
95-99	39.717961967953435	41.0	40.0	41.0	37.0	41.0
100-104	39.60455451651224	41.0	39.0	41.0	36.4	41.0
105-109	39.451593228985395	40.6	39.0	41.0	36.0	41.0
110-114	39.25491011673915	40.0	38.6	41.0	35.2	41.0
115-119	38.995133356008424	40.0	38.0	41.0	35.0	41.0
120-124	38.69609986346584	40.0	37.6	41.0	35.0	41.0
125-129	38.254768243345524	39.6	36.6	40.6	34.0	41.0
130-134	38.04465560539707	39.0	35.6	41.0	34.0	41.0
135-139	37.71132008859927	38.8	35.0	41.0	34.0	41.0
140-144	37.25449899308564	37.2	35.0	40.2	34.0	41.0
145-149	36.5963367741375	36.0	35.0	39.0	34.0	41.0
150-154	35.959184815671584	35.0	35.0	37.4	33.8	39.8
155-159	35.41076299042852	35.0	35.0	36.2	33.0	38.4
160-164	34.999641556656414	35.0	35.0	35.8	33.0	37.0
165-169	34.72822485513346	35.0	35.0	35.0	33.0	36.0
170-174	34.57118825152218	35.0	35.0	35.0	33.0	36.0
175-178	34.26674734420756	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	52.0
30	496.0
31	3637.0
32	18634.0
33	68663.0
34	194184.0
35	471597.0
36	1105449.0
37	2003417.0
38	2254485.0
39	746416.0
40	22031.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.15451910448212	28.444880175303933	27.87660268506723	21.523998035146718
2	22.51765987367805	29.16666182807998	27.95831189839432	20.357366399847642
3	20.35502936247943	28.17674505564181	29.708665792624366	21.75955978925439
4	21.146936652061875	27.23430933433047	30.02074011796087	21.59801389564678
5	22.00579062699955	27.98500638112812	28.459439482635084	21.54976350923725
6	21.897749824939932	27.9745260023713	28.47296817100262	21.654756001686152
7	22.09384322746893	28.06532208149032	27.99374486867882	21.847089822361934
8	22.2063693993843	28.374957759138255	27.797157930308092	21.621514911169353
9	22.11181373841207	28.299809669354207	27.986080547371895	21.602296044861827
10-14	22.151479504327437	28.340433475432945	27.80128911561861	21.706797904621006
15-19	22.02442595975302	28.3098255437894	27.974253106082337	21.691495390375238
20-24	21.959334388532316	28.253971802265156	28.0926117103862	21.694082098816327
25-29	21.991161644020146	28.29518204504995	27.899671130940284	21.81398517998962
30-34	21.991004873811594	28.302277348562882	27.973393773087317	21.733324004538208
35-39	21.944969592385846	28.348411337156975	27.99000270573767	21.716616364719503
40-44	21.96224624999855	28.3409879774669	27.97024095000424	21.72652482253032
45-49	22.046757005015486	28.267512103240733	27.930711632233347	21.755019259510437
50-54	21.992212585047838	28.38766775863891	27.96726521919378	21.65285443711947
55-59	22.0054364424543	28.309578775868538	27.93445960147852	21.750525180198647
60-64	21.928003572038232	28.31336158293783	28.015663666355834	21.742971178668103
65-69	21.914564881383015	28.343882420021068	28.04572870857347	21.695823990022447
70-74	21.877369697828325	28.35162512643227	28.000123093645236	21.77088208209417
75-79	21.85930918917287	28.33888609541151	28.038258898451225	21.763545816964395
80-84	21.849392021906024	28.418821482860373	28.013712748204984	21.718073747028622
85-89	21.889830026256107	28.36482285547064	27.951448847042208	21.79389827123104
90-94	21.425776981142825	28.123997570023352	28.841854672331657	21.60837077650217
95-99	21.763959953455995	28.15741193135099	28.182470600468797	21.89615751472422
100-104	21.826413682683103	28.24058582800036	28.044599913440937	21.888400575875604
105-109	21.692549279854713	28.23358730479179	28.184542780213167	21.889320635140326
110-114	21.69231878584461	28.05740370987101	28.273924231224097	21.976353273060283
115-119	21.671619918053974	28.18136534068083	28.173283332169074	21.97373140909612
120-124	21.61903367904126	28.15745863638209	28.205888165565284	22.017619519011365
125-129	21.57421313397413	28.170425794735028	28.27043366969943	21.98492740159141
130-134	21.651051920896222	28.210598352105166	28.20669475317598	21.931654973822635
135-139	21.720086242117702	28.161046246438094	28.17193007165313	21.946937439791075
140-144	21.644700442608414	28.233699868509067	28.282097393542248	21.83950229534027
145-149	21.580132966738525	28.30866752621845	28.3083712936875	21.802828213355525
150-154	21.50583437962271	28.37577762923643	28.367299149810734	21.75108884133013
155-159	21.457922436562534	28.375540816104284	28.44069470288427	21.72584204444892
160-164	21.375265960262098	28.56208412731932	28.479535300881594	21.583114611536995
165-169	21.293784277709662	28.58340859076324	28.52596729387895	21.596839837648144
170-174	21.215552989364642	28.65522225423531	28.632602617072344	21.496622139327705
175-178	21.174084336950976	28.791072088057334	28.608329490013553	21.426514084978134
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	3.0
7	13.5
8	26.5
9	57.0
10	132.5
11	244.5
12	402.5
13	685.0
14	1148.5
15	1795.0
16	2650.5
17	3997.0
18	6008.5
19	8780.0
20	12837.5
21	18256.0
22	24797.5
23	32573.0
24	41439.0
25	51267.5
26	62113.0
27	73746.5
28	86292.5
29	99991.5
30	114581.5
31	129263.0
32	143744.5
33	159238.5
34	174733.5
35	190596.0
36	206521.5
37	221485.0
38	235795.0
39	248789.5
40	259608.0
41	267318.0
42	272105.5
43	273879.0
44	272529.0
45	267087.5
46	257272.0
47	244513.5
48	229487.0
49	212663.0
50	195355.5
51	178630.0
52	162977.5
53	148450.5
54	135472.5
55	124764.0
56	116342.5
57	108538.0
58	101073.0
59	94558.0
60	88152.0
61	81609.0
62	74871.0
63	67431.5
64	59315.5
65	51246.5
66	43633.5
67	36294.5
68	29263.0
69	22894.0
70	17600.0
71	13243.0
72	9475.0
73	6517.5
74	4413.5
75	2867.0
76	1778.0
77	1100.0
78	677.5
79	381.0
80	204.0
81	106.0
82	51.5
83	25.5
84	17.0
85	8.5
86	3.5
87	1.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1088.0
92-93	2426.0
94-95	3402.0
96-97	4333.0
98-99	5345.0
100-101	7884.0
102-103	7342.0
104-105	8855.0
106-107	9368.0
108-109	10349.0
110-111	10788.0
112-113	12140.0
114-115	13740.0
116-117	13319.0
118-119	13735.0
120-121	14502.0
122-123	15763.0
124-125	16724.0
126-127	16090.0
128-129	17651.0
130-131	18259.0
132-133	19455.0
134-135	91486.0
136-137	78943.0
138-139	66038.0
140-141	68833.0
142-143	80728.0
144-145	80209.0
146-147	74590.0
148-149	77720.0
150-151	74835.0
152-153	70997.0
154-155	76661.0
156-157	76370.0
158-159	81868.0
160-161	74403.0
162-163	71843.0
164-165	73869.0
166-167	77668.0
168-169	84099.0
170-171	79247.0
172-173	86662.0
174-175	94283.0
176-177	207572.0
178-179	4797582.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.38665885496562
#Duplication Level	Percentage of total
1	91.48297931007968
2	6.8078597646479055
3	1.134480600727735
4	0.31147203087950176
5	0.12240823827917761
6	0.05180484817385456
7	0.034438426507235505
8	0.021022316072647422
9	0.011645461485774207
>10	0.021889003146472248
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.903152010200515E-5	0.0	0.0	0.0	2.903152010200515E-5	0.0
2	4.4998856158107983E-4	0.0	1.4515760051002574E-5	0.0	5.80630402040103E-5	0.0
3	4.645043216320824E-4	0.0	1.4515760051002574E-5	0.0	5.80630402040103E-5	0.0
4	4.645043216320824E-4	0.0	2.903152010200515E-5	0.0	7.257880025501287E-5	0.0
5	4.645043216320824E-4	1.4515760051002574E-5	4.354728015300772E-5	0.0	8.709456030601544E-5	0.0
6	4.7902008168308497E-4	2.903152010200515E-5	4.354728015300772E-5	0.0	1.161260804080206E-4	0.0
7	4.7902008168308497E-4	7.257880025501287E-5	5.80630402040103E-5	0.0	1.161260804080206E-4	0.0
8	4.7902008168308497E-4	7.257880025501287E-5	5.80630402040103E-5	0.0	1.4515760051002575E-4	0.0
9	4.7902008168308497E-4	7.257880025501287E-5	7.257880025501287E-5	0.0	1.7418912061203088E-4	0.0
10-14	4.84826385703486E-4	8.999771231621595E-5	8.709456030601544E-5	4.06441281428072E-5	2.1483324875483812E-4	0.0
15-19	5.080516017850901E-4	1.1031977638761956E-4	1.393512964896247E-4	8.999771231621595E-5	3.4257193720366077E-4	0.0
20-24	5.225673618360927E-4	1.4225444849982525E-4	1.6547966458142936E-4	1.1031977638761956E-4	4.90632689723887E-4	0.0
25-29	5.225673618360927E-4	1.5967336056102831E-4	2.3515531282624174E-4	1.97414336693635E-4	7.199816985297276E-4	0.0
30-34	6.648218103359178E-4	2.0902694473443707E-4	2.583805289078458E-4	2.322521608160412E-4	0.0010306189636211827	1.161260804080206E-5
35-39	0.0010799725477945916	2.6128368091804635E-4	2.757994409690489E-4	2.6708998493844735E-4	0.0014283507890186533	1.4515760051002574E-5
40-44	0.001416738180977851	2.903152010200515E-4	2.8450889699965044E-4	2.8450889699965044E-4	0.001826082614416124	1.4515760051002574E-5
45-49	0.0020089811910587565	3.1644356911185613E-4	2.9902465705065304E-4	3.019278090608536E-4	0.0021657513996095846	4.354728015300772E-5
50-54	0.0025576769209866537	3.367656331832598E-4	3.512813932342623E-4	3.512813932342623E-4	0.002560580072996854	6.677249623461185E-5
55-59	0.0028944425541699136	3.8611921735666845E-4	3.6289400127506436E-4	3.9192552137706956E-4	0.0029234740742719186	9.580401633661699E-5
60-64	0.003205079819261368	4.267633454994757E-4	3.6289400127506436E-4	4.616011696218818E-4	0.0033850752438938	1.0161032035701802E-4
65-69	0.0035070076283222223	4.790200816830849E-4	3.8321606534646795E-4	5.051484497748896E-4	0.0037392597891382634	1.0741662437741906E-4
70-74	0.006064684549308875	5.138579058054911E-4	4.267633454994757E-4	5.603083379686994E-4	0.0041021537904133275	1.161260804080206E-4
75-79	0.052866398105751375	5.370831218870953E-4	4.383759535402778E-4	6.125650741523087E-4	0.004389565839423179	1.2193238442842162E-4
80-84	0.07343813325003223	5.922430100809052E-4	4.732137776626839E-4	6.503060502849152E-4	0.004639236912300422	1.3064184045902318E-4
85-89	0.07366457910682786	6.183713781727097E-4	4.877295377136865E-4	6.909501784277225E-4	0.004973099393473483	1.3064184045902318E-4
90-94	0.07377489888321549	6.619186583257175E-4	4.935358417340875E-4	7.432069146113319E-4	0.0050747097138305	1.3064184045902318E-4
95-99	0.07384167137945011	7.025627864685245E-4	5.109547537952906E-4	7.66432130692936E-4	0.00518212633820792	1.3064184045902318E-4
100-104	0.07400134474001112	7.141753945093266E-4	5.225673618360927E-4	8.215920188867456E-4	0.005234383074391528	1.3064184045902318E-4
105-109	0.07428004733299039	7.286911545603292E-4	5.486957299278973E-4	8.419140829581493E-4	0.005318574482687344	1.3064184045902318E-4
110-114	0.07443101123752079	7.548195226521339E-4	5.748240980197019E-4	8.56429843009152E-4	0.005428894259074962	1.3064184045902318E-4
115-119	0.07464294133426544	7.983668028051416E-4	6.096619221421082E-4	8.883645151213576E-4	0.005600180227676793	1.3064184045902318E-4
120-124	0.07520034652022393	8.448172349683498E-4	6.357902902339128E-4	9.69652771406972E-4	0.005875979668645842	1.364481444794242E-4
125-129	0.07606258266725349	8.85461363111157E-4	6.648218103359178E-4	0.0010132000515599795	0.006119844437502686	1.4515760051002575E-4
130-134	0.07691030305423205	9.057834271825607E-4	6.793375703869205E-4	0.0010625536357333884	0.006328871382237122	1.4515760051002575E-4
135-139	0.07781898963342479	9.435244033151673E-4	6.851438744073215E-4	0.0010799725477945916	0.006564026695063364	1.5967336056102831E-4
140-144	0.07862316274025034	9.75459075427373E-4	7.141753945093266E-4	0.001106100915886396	0.0068224072239712105	1.5967336056102831E-4
145-149	0.07932862867872907	9.928779874885762E-4	7.983668028051416E-4	0.0011322292839782008	0.007008208952624042	1.5967336056102831E-4
150-154	0.08009796396143219	0.0010190063555803807	8.62236147029553E-4	0.001167067108100607	0.00721433274534828	1.5967336056102831E-4
155-159	0.081122776621033	0.0010306189636211827	8.825582111009567E-4	0.0011902923241822112	0.0074930353383275295	1.6838281659162986E-4
160-164	0.08204597896027675	0.0010451347236721854	8.912676671315581E-4	0.0011902923241822112	0.0077775442353271804	1.7418912061203088E-4
165-167	0.08282208826433703	0.0010451347236721854	9.048157098458272E-4	0.0012338396043352188	0.007925604987847406	1.7418912061203086E-4
>>END_MODULE
