##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478989
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7914340
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.217490403495425	39.0	39.0	39.0	37.0	39.0
2	39.962611032631905	41.0	40.0	41.0	38.0	41.0
3	40.00988749535653	41.0	40.0	41.0	38.0	41.0
4	40.02847123070275	41.0	40.0	41.0	38.0	41.0
5	40.04817243130823	41.0	40.0	41.0	38.0	41.0
6	40.03029968386498	41.0	40.0	41.0	38.0	41.0
7	40.047124333804206	41.0	40.0	41.0	38.0	41.0
8	40.035039182041714	41.0	40.0	41.0	38.0	41.0
9	40.015414677661056	41.0	40.0	41.0	38.0	41.0
10-14	39.951154714101236	41.0	40.0	41.0	38.0	41.0
15-19	39.82363868623284	41.0	40.0	41.0	37.8	41.0
20-24	39.63895895298913	40.6	39.4	41.0	37.0	41.0
25-29	39.40834725321378	40.0	39.0	41.0	36.2	41.0
30-34	39.31901245081712	40.0	38.8	41.0	35.6	41.0
35-39	39.16054506124326	40.0	38.2	41.0	35.0	41.0
40-44	38.68343139162583	40.0	36.8	41.0	35.0	41.0
45-49	38.087261906867795	39.0	35.4	41.0	34.8	41.0
50-54	37.43553974178516	37.4	35.0	40.2	34.0	41.0
55-59	36.75692323049047	36.4	35.0	39.2	34.0	41.0
60-64	36.01900294907725	35.4	34.8	38.0	33.4	40.0
65-69	35.60496940995712	35.0	35.0	36.8	33.0	39.0
70-74	35.324246317444036	35.0	35.0	36.0	33.0	38.2
75-79	35.108985613456085	35.0	35.0	35.6	33.0	37.0
80-84	35.001491217208255	35.0	35.0	35.0	33.0	37.0
85-89	34.80752219389109	35.0	34.6	35.0	32.6	37.2
90-94	39.462490987002795	40.6	39.8	40.6	36.8	40.6
95-99	39.73875315085537	41.0	40.0	41.0	37.0	41.0
100-104	39.622864859039396	41.0	39.2	41.0	36.6	41.0
105-109	39.47754836951617	40.8	39.0	41.0	36.0	41.0
110-114	39.28279741138669	40.0	38.6	41.0	35.2	41.0
115-119	39.01716096882165	40.0	38.0	41.0	35.0	41.0
120-124	38.719660355952826	40.0	37.8	41.0	35.0	41.0
125-129	38.27014562531174	39.6	36.6	40.6	34.2	41.0
130-134	38.06190888795182	39.0	35.6	41.0	34.0	41.0
135-139	37.73174725041548	38.8	35.0	41.0	34.0	41.0
140-144	37.26578781085385	37.2	35.0	40.2	34.0	41.0
145-149	36.60733089331709	36.0	35.0	39.0	34.0	41.0
150-154	35.963368733916596	35.0	35.0	37.4	33.8	39.8
155-159	35.42171446014031	35.0	35.0	36.2	33.0	38.4
160-164	35.00740559870954	35.0	35.0	35.8	33.0	37.0
165-169	34.73666714089863	35.0	35.0	35.0	33.0	36.0
170-174	34.57332322065168	35.0	35.0	35.0	33.0	36.0
175-178	34.260798692695985	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	45.0
30	551.0
31	4016.0
32	20720.0
33	76701.0
34	219018.0
35	533078.0
36	1259791.0
37	2298345.0
38	2611678.0
39	865752.0
40	24643.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.164059163493103	28.441980000859203	27.880770853918328	21.513189981729365
2	22.52240869105952	29.151161057018022	28.007553377792714	20.318876874129742
3	20.374598513584203	28.17500890788114	29.698067052969673	21.752325525564984
4	21.13401749229879	27.213387344996548	30.025775996482334	21.626819166222326
5	21.958457685669305	27.998594955485867	28.474958114005716	21.567989244839115
6	21.907979692558065	27.98245968709962	28.467490656201278	21.64206996414104
7	22.117270675760707	28.03972283222606	27.986187098355643	21.856819393657588
8	22.185349631175814	28.390832337251116	27.814372392391533	21.609445639181537
9	22.090458585302123	28.317812982510226	28.00618876621424	21.58553966597341
10-14	22.155194242349964	28.32655660484639	27.810172421199997	21.708076731603647
15-19	22.035002286987922	28.321959885473706	27.96789372202862	21.675144105509744
20-24	21.97370848358802	28.255531099245168	28.08105034658607	21.68971007058074
25-29	21.992598245716007	28.293055390594795	27.898035717444536	21.816310646244666
30-34	21.98828202983445	28.291458289636278	27.9901950131028	21.73006466742647
35-39	21.941119537447214	28.358253499344226	27.99619172287266	21.704435240335897
40-44	21.95735336111413	28.35423294930468	27.9789571840482	21.709456505532994
45-49	22.037675914858347	28.266399977761886	27.947346209538637	21.748577897841134
50-54	21.993500405592886	28.3860031284984	27.96826014550803	21.65223632040069
55-59	21.99941372243295	28.314361020628375	27.93094560001213	21.75527965692654
60-64	21.931686028146377	28.33470889549855	28.011455156083763	21.722149920271306
65-69	21.91433019051494	28.34743010788013	28.026572019903114	21.711667681701822
70-74	21.889383069213604	28.375389482888025	27.98081709908849	21.754410348809884
75-79	21.87036442710321	28.338512118508934	28.038231867723653	21.75289158666421
80-84	21.86447638084793	28.413090668331154	27.992444095148805	21.729988855672108
85-89	21.89233720057516	28.380991971535213	27.93381381138541	21.792857016504218
90-94	21.409186926116632	28.128466060792732	28.85752772355762	21.60481928953302
95-99	21.770381672930185	28.14468746784887	28.187660230612146	21.897270628608805
100-104	21.810500434882087	28.258753117601525	28.04805132663377	21.882695120882616
105-109	21.69199375016974	28.214376786321495	28.196045042637767	21.897584420871
110-114	21.68287825800871	28.05687203549559	28.28250047259545	21.977749233900255
115-119	21.662930686587384	28.18959075012647	28.169989477130404	21.977489086155742
120-124	21.62880958760727	28.161095823667175	28.209859987157433	22.00023460156812
125-129	21.573826073567464	28.166868371060378	28.26894651015928	21.99035904521288
130-134	21.645908132627998	28.215753302890693	28.21176791441886	21.926570650062445
135-139	21.699727279791023	28.193945397562608	28.160128602212005	21.94619872043437
140-144	21.64594248796506	28.227169821592042	28.299313607855574	21.827574082587322
145-149	21.585660426744024	28.310677512923014	28.316610624224637	21.787051436108325
150-154	21.505221477042408	28.375895903041737	28.366978329484517	21.75190429043134
155-159	21.42860153980836	28.388273662692377	28.466895597204555	21.716229200294705
160-164	21.359149282752423	28.547662633931164	28.488831360314613	21.604356723001796
165-169	21.278970549355492	28.562080710623803	28.54429137087448	21.614657369146226
170-174	21.218081222878695	28.681035290584827	28.61883530240276	21.482048184133717
175-178	21.183058069821914	28.782438002254057	28.603411014324287	21.43109291359974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	4.0
7	14.5
8	37.0
9	70.0
10	143.0
11	276.0
12	480.0
13	776.5
14	1220.0
15	2020.5
16	3173.5
17	4685.5
18	6878.5
19	10117.0
20	14686.0
21	20746.5
22	28450.5
23	37442.0
24	47594.0
25	58831.5
26	71283.0
27	84737.5
28	98859.5
29	114550.0
30	131090.0
31	148001.0
32	165526.0
33	183263.0
34	200954.5
35	219232.0
36	237038.0
37	254071.5
38	270807.5
39	285318.0
40	297573.0
41	307315.5
42	313726.0
43	315934.5
44	313246.0
45	306597.5
46	296391.5
47	281784.0
48	263690.5
49	244637.5
50	225240.0
51	205879.0
52	187474.5
53	170638.0
54	156100.0
55	143522.5
56	132984.0
57	123889.0
58	115685.0
59	108428.0
60	101091.0
61	93351.0
62	85501.5
63	77061.5
64	68077.5
65	58966.0
66	49967.5
67	41354.5
68	33329.5
69	26313.0
70	20107.5
71	14868.5
72	10804.5
73	7603.0
74	5094.5
75	3370.5
76	2173.0
77	1301.5
78	778.5
79	444.0
80	243.5
81	135.0
82	62.5
83	30.5
84	18.0
85	7.0
86	2.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1216.0
92-93	2495.0
94-95	3763.0
96-97	4997.0
98-99	6649.0
100-101	6785.0
102-103	8024.0
104-105	9509.0
106-107	11344.0
108-109	11049.0
110-111	11735.0
112-113	12879.0
114-115	13399.0
116-117	13695.0
118-119	14362.0
120-121	16159.0
122-123	16082.0
124-125	16577.0
126-127	17657.0
128-129	18137.0
130-131	19667.0
132-133	20682.0
134-135	97058.0
136-137	80952.0
138-139	79429.0
140-141	78190.0
142-143	86505.0
144-145	81622.0
146-147	86620.0
148-149	85357.0
150-151	82706.0
152-153	83400.0
154-155	83759.0
156-157	83757.0
158-159	81637.0
160-161	85123.0
162-163	81349.0
164-165	89985.0
166-167	86302.0
168-169	91407.0
170-171	95798.0
172-173	111236.0
174-175	104585.0
176-177	245136.0
178-179	5575565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.80649792791225
#Duplication Level	Percentage of total
1	90.44976973155285
2	7.53452422427577
3	1.3428315201913812
4	0.3564209992335969
5	0.14654467562508405
6	0.06600044756589686
7	0.04047236164402949
8	0.025062613059291385
9	0.008406600248893567
>10	0.029966826603195152
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2635292393301274E-5	1.2635292393301274E-5	0.0	0.0	0.0	0.0
2	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	1.2635292393301274E-5	0.0	0.0
3	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	1.2635292393301274E-5	0.0	0.0
4	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	1.2635292393301274E-5	2.527058478660255E-5	0.0
5	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	1.2635292393301274E-5	2.527058478660255E-5	0.0
6	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	1.2635292393301274E-5	7.581175435980763E-5	0.0
7	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	3.790587717990382E-5	7.581175435980763E-5	0.0
8	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	5.05411695732051E-5	1.010823391464102E-4	0.0
9	5.180469881253522E-4	2.527058478660255E-5	2.527058478660255E-5	6.317646196650637E-5	1.2635292393301274E-4	0.0
10-14	5.180469881253522E-4	5.55952865305256E-5	6.064940348784611E-5	7.32846958811474E-5	1.7436703502755758E-4	0.0
15-19	5.256281635613329E-4	1.1624469001837173E-4	1.3646115784765375E-4	9.097410523176917E-5	2.6281408178066643E-4	0.0
20-24	5.382634559546342E-4	1.4151527480497426E-4	1.38988216326314E-4	1.2382586545435249E-4	4.1696464897894207E-4	0.0
25-29	5.483716898692753E-4	1.5920468415559606E-4	1.5415056719827555E-4	1.5415056719827552E-4	5.508987483479356E-4	0.0
30-34	7.025222570675508E-4	1.8700232742085884E-4	1.743670350275576E-4	1.819482104635383E-4	9.779716312415187E-4	0.0
35-39	0.001180136309534339	2.021646782928204E-4	1.7689409350621783E-4	2.375434969940639E-4	0.0014758021515375887	1.010823391464102E-5
40-44	0.0015844656661199799	2.3501643851540368E-4	2.2996232155808317E-4	2.906117250459293E-4	0.0019458350285683963	2.2743526307942292E-5
45-49	0.0020696608940227483	2.855576080886088E-4	2.527058478660255E-4	3.184093683111921E-4	0.002264244396879588	2.527058478660255E-5
50-54	0.0025826537651907804	3.512611285337754E-4	2.527058478660255E-4	3.512611285337754E-4	0.0025396937710535556	3.0324701743923058E-5
55-59	0.0029591854785111582	4.018022981069805E-4	2.729223156953075E-4	3.5631524549109594E-4	0.002799980794355562	4.2959994137224324E-5
60-64	0.003307919548566273	4.5234346768018556E-4	3.0830113439655105E-4	3.689505378843972E-4	0.0031790395661546005	5.05411695732051E-5
65-69	0.003590950098176222	4.927764033387497E-4	3.285176022258331E-4	3.9674818114966E-4	0.003699613612758613	5.05411695732051E-5
70-74	0.006320173255129296	5.433175729119547E-4	3.714775963630574E-4	4.675058185521471E-4	0.00416964648978942	5.05411695732051E-5
75-79	0.053773277367411554	5.86277567049179E-4	4.119105320216215E-4	5.028846372533907E-4	0.0044526770393993696	5.05411695732051E-5
80-84	0.07452295453569091	5.989128594424805E-4	4.321269998509036E-4	5.483716898692754E-4	0.004730653472051997	5.05411695732051E-5
85-89	0.07474028156485568	6.292375611864034E-4	4.498164092015253E-4	5.786963916131983E-4	0.005122347536244336	5.05411695732051E-5
90-94	0.07483378272856614	6.64616379887647E-4	4.902493448600894E-4	6.039669763998009E-4	0.005188051056689503	5.05411695732051E-5
95-99	0.07490454036596862	6.898869646742496E-4	5.256281635613329E-4	6.292375611864034E-4	0.005268916928006631	5.05411695732051E-5
100-104	0.07502836623142295	7.075763740248713E-4	5.35736397475974E-4	6.873599061955893E-4	0.005369999267153041	5.05411695732051E-5
105-109	0.07512944857056937	7.454822512047751E-4	5.433175729119547E-4	7.202116664181726E-4	0.0054382298460768684	5.05411695732051E-5
110-114	0.07528359913776764	7.581175435980764E-4	5.534258068265957E-4	7.404281342474545E-4	0.005531731009787298	5.05411695732051E-5
115-119	0.07540489794474334	7.909693038206597E-4	5.963858009638202E-4	7.682257775127174E-4	0.005695989810900214	5.05411695732051E-5
120-124	0.07585724141242353	8.389834149152046E-4	6.368187366223842E-4	8.111857716499417E-4	0.005963858009638201	5.05411695732051E-5
125-129	0.07653702014318313	8.819434090524289E-4	6.721975553236278E-4	8.566728242658264E-4	0.006345443839915898	5.55952865305256E-5
130-134	0.07748972118963805	9.198492862323328E-4	6.84832847716929E-4	8.718351751377879E-4	0.006560243810602021	6.317646196650637E-5
135-139	0.07824025755780015	9.249034031896532E-4	7.328469588114738E-4	8.794163505737686E-4	0.006903923763699815	6.317646196650637E-5
140-144	0.07903628097857814	9.350116371042944E-4	7.656987190340572E-4	8.971057599243904E-4	0.007181900196352444	6.317646196650637E-5
145-149	0.07990558909523725	9.52701046454916E-4	7.707528359913777E-4	9.451198710189353E-4	0.00743713310269713	6.317646196650637E-5
150-154	0.08060053017686881	9.653363388482172E-4	7.985504792566404E-4	0.001000715157549461	0.0077353260031790385	6.317646196650637E-5
155-159	0.08121965950414059	9.729175142841981E-4	8.314022394792236E-4	0.0010386210347293648	0.008058789488447552	6.317646196650637E-5
160-164	0.08207380526992776	9.80498689720179E-4	8.516187073085059E-4	0.0010739998534306083	0.008399942383066687	6.317646196650637E-5
165-167	0.08290015339244966	9.981880990708005E-4	8.844704675310891E-4	0.001082423381692809	0.008625692940493668	7.581175435980765E-5
>>END_MODULE
