##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR478998
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4222017
Total Bases	654 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.808212993931576	39.0	37.0	39.0	35.0	39.0
2	39.37592956162896	40.0	39.0	41.0	37.0	41.0
3	39.42859727945198	40.0	39.0	41.0	37.0	41.0
4	39.41921148114752	40.0	39.0	41.0	37.0	41.0
5	39.42257527622461	40.0	39.0	41.0	37.0	41.0
6	39.38989066126451	40.0	39.0	41.0	37.0	41.0
7	39.42465319301178	40.0	39.0	41.0	37.0	41.0
8	39.39268553395214	40.0	39.0	41.0	37.0	41.0
9	39.355540728519095	40.0	39.0	41.0	37.0	41.0
10-14	39.20525232371163	40.0	38.6	41.0	36.2	41.0
15-19	39.065787750262494	40.0	38.0	41.0	36.0	41.0
20-24	39.13470680009105	40.0	38.0	41.0	36.0	41.0
25-29	38.990355036467164	40.0	38.0	41.0	35.6	41.0
30-34	38.669201568823624	40.0	38.0	40.4	35.0	41.0
35-39	38.27248251250528	39.8	37.0	40.0	34.0	41.0
40-44	37.65374540178308	39.0	35.6	40.0	33.0	41.0
45-49	36.96699624847555	38.0	35.0	40.0	32.0	41.0
50-54	36.42584527726913	37.0	34.2	39.6	31.8	40.6
55-59	35.98615619027588	36.0	34.0	39.0	31.0	40.0
60-64	35.52025598191575	35.4	33.8	38.8	30.8	40.0
65-69	35.52771777091376	35.0	33.8	39.0	31.0	40.0
70-74	35.55254363021276	35.0	34.0	39.0	31.0	40.6
75-79	35.62303211948223	35.0	34.0	39.0	31.0	41.0
80-84	35.810695456697594	35.0	34.0	39.4	31.0	41.0
85-89	35.98059832539756	35.6	33.8	40.0	30.6	41.0
90-94	38.841874011081245	39.6	38.0	40.6	35.0	41.0
95-99	38.92165521923515	40.0	38.0	41.0	35.0	41.0
100-104	38.68105074517554	40.0	38.0	41.0	34.4	41.0
105-109	38.51083389775655	40.0	37.4	41.0	34.0	41.0
110-114	38.33462534568472	40.0	36.8	41.0	34.0	41.0
115-119	38.08665514305013	39.6	36.0	41.0	33.4	41.0
120-124	37.78720555583264	39.0	35.2	41.0	33.0	41.0
125-129	37.35825039700181	38.8	35.0	40.6	32.8	41.0
130-134	37.10578846194305	38.0	35.0	40.0	32.8	41.0
135-139	36.796331612528306	37.2	35.0	40.0	32.0	41.0
140-144	36.33105683396856	36.2	34.4	39.4	32.0	41.0
145-149	35.82985481996775	35.4	34.0	38.8	31.8	40.6
150-154	35.289737999372775	35.0	34.0	37.2	31.0	39.6
155-159	34.76287735094405	35.0	34.0	36.2	31.0	38.8
160-164	34.31270590243143	35.0	34.0	35.4	31.0	37.0
165-169	33.9618385370989	35.0	33.2	35.0	31.0	36.0
170-174	33.66602758492824	34.6	33.0	35.0	30.0	35.8
175-179	33.16850278227496	34.0	32.6	34.8	29.4	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	27.0
31	533.0
32	5420.0
33	33794.0
34	140359.0
35	438517.0
36	1028991.0
37	1482494.0
38	934091.0
39	156340.0
40	1451.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.896694636710368	29.053720058445997	28.68787596070788	21.361709344135754
2	20.968413912118308	28.881811702795133	29.615465783297413	20.534308601789146
3	20.407426118843198	27.984468087172555	29.56530018709067	22.04280560689358
4	20.775046618713283	27.794535171222662	29.661960148431426	21.76845806163263
5	21.139824875172224	28.38361380354461	28.998746333802067	21.477814987481104
6	21.561519055939378	28.203320829830858	28.437379574738802	21.79778053949096
7	21.34574541030981	28.674233192334377	28.42890021522888	21.55112118212693
8	21.11216037263706	28.859855372444027	28.683494168782364	21.344490086136556
9	21.549060555653853	28.57233876604476	28.228166774316637	21.65043390398475
10-14	21.23830860936846	28.89285381844744	28.34574564716343	21.523091925020672
15-19	21.314021236769058	28.866899399031315	28.281577265084433	21.537502099115187
20-24	21.256138949701057	28.811016156495818	28.416484348594523	21.516360545208606
25-29	21.160743786678264	28.857439465544548	28.43511051708224	21.54670623069495
30-34	21.25198927432078	28.747013571949143	28.4084976446092	21.592499509120877
35-39	21.180682124207458	28.701954539737763	28.530813589807906	21.586549746246877
40-44	21.293964472431067	28.625488717833207	28.479345298704388	21.60120151103134
45-49	21.84679976418854	28.32644681440174	28.144822723357105	21.68193069805261
50-54	21.680353252959428	28.33524355775924	28.327815828311444	21.656587360969887
55-59	21.55553139648656	28.416588564186263	28.336167286867862	21.69171275245931
60-64	21.471400991516614	28.38709081465091	28.3589289195188	21.782579274313676
65-69	21.314750745911255	28.422310947587377	28.52743605722099	21.73550224928038
70-74	21.310250527176937	28.470766460675073	28.549657663623805	21.669325348524175
75-79	21.43476447394693	28.41734649576257	28.399639319311127	21.748249710979373
80-84	21.502130379863463	28.438829118878488	28.33744155933053	21.72159894192752
85-89	21.592935319777254	28.344627698088377	28.304893135200544	21.757543846933824
90-94	21.106433767411414	28.348347175165767	28.871204653560046	21.674014403862778
95-99	21.3402605651819	28.458149515200525	28.44747881778395	21.754111101833626
100-104	21.333096568367303	28.557438164537878	28.39319571393764	21.71626955315718
105-109	21.34765672303044	28.537884512777804	28.425488046622128	21.68897071756963
110-114	21.306031483602688	28.56987336284052	28.522255716503185	21.601839437053602
115-119	21.221345101881976	28.63832373235684	28.59230712680128	21.548024038959905
120-124	21.21985007238452	28.701547197620076	28.62312688962089	21.455475840374515
125-129	21.190505783867746	28.743497557432505	28.715605851191796	21.350390807507953
130-134	21.16304998415623	28.823693919570903	28.816990015992616	21.196266080280246
135-139	21.19190458914794	28.87282177905673	28.823755359976268	21.111518271819065
140-144	21.05044446385142	29.006731133111565	29.037164799627384	20.905659603409628
145-149	20.848092827938707	29.20066036841378	29.184708461531223	20.76653834211629
150-154	20.71910040131872	29.3452461952916	29.384687268984283	20.550966134405392
155-159	20.512456051864948	29.567718415161952	29.64322739495973	20.27659813801337
160-164	20.430786471623808	29.763253516621706	29.86340060766167	19.942559404092812
165-169	20.453294186642804	29.96227113410319	29.83769360441404	19.746741074839967
170-174	20.47863973331325	30.239402008364763	29.936066370500175	19.345891887821807
175-179	20.4209020016461	30.425408684422745	30.1361288391672	19.01756047476396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.5
6	2.0
7	3.0
8	7.5
9	18.5
10	37.5
11	81.5
12	161.0
13	276.5
14	475.5
15	825.0
16	1348.0
17	2153.0
18	3304.5
19	4855.0
20	7066.5
21	9910.5
22	13350.0
23	17421.5
24	22334.5
25	28055.0
26	34310.0
27	41621.5
28	49541.0
29	57744.0
30	66858.5
31	76445.0
32	86158.0
33	96290.5
34	106599.5
35	117468.5
36	128323.0
37	138524.5
38	148489.5
39	157766.0
40	166534.0
41	173804.0
42	178507.5
43	181055.5
44	181540.0
45	179403.0
46	174661.0
47	168076.0
48	159805.5
49	149965.5
50	138867.0
51	127088.5
52	114848.5
53	103059.5
54	92124.5
55	81347.0
56	71540.0
57	62785.5
58	54397.0
59	46690.0
60	40089.0
61	34003.5
62	28357.0
63	23302.0
64	18694.5
65	14858.5
66	11530.5
67	8749.5
68	6511.0
69	4717.0
70	3290.0
71	2219.0
72	1516.5
73	967.0
74	583.5
75	353.0
76	208.0
77	110.5
78	63.5
79	30.5
80	14.5
81	7.5
82	3.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2780.0
92-93	6171.0
94-95	9580.0
96-97	13020.0
98-99	16738.0
100-101	20375.0
102-103	23451.0
104-105	26728.0
106-107	30276.0
108-109	33085.0
110-111	35871.0
112-113	38816.0
114-115	41570.0
116-117	43940.0
118-119	46859.0
120-121	49621.0
122-123	52355.0
124-125	55410.0
126-127	57759.0
128-129	60156.0
130-131	63292.0
132-133	66104.0
134-135	93375.0
136-137	103789.0
138-139	103332.0
140-141	104432.0
142-143	104801.0
144-145	103008.0
146-147	103868.0
148-149	104722.0
150-151	105958.0
152-153	102589.0
154-155	96314.0
156-157	97991.0
158-159	99775.0
160-161	103885.0
162-163	103994.0
164-165	104853.0
166-167	106510.0
168-169	110684.0
170-171	113302.0
172-173	113512.0
174-175	123261.0
176-177	118248.0
178-179	1105857.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.46607382986329
#Duplication Level	Percentage of total
1	93.31804304688052
2	5.701930370841062
3	0.7368489765378958
4	0.14396786808933149
5	0.041927036455152875
6	0.01909275955599014
7	0.008586710829291572
8	0.006516394831346497
9	0.0054944481652930794
>10	0.01759238781411472
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.3685361759557102E-4	0.0	0.0	0.0	0.0	0.0
2	3.0790970287424235E-4	2.3685361759557102E-5	0.0	0.0	0.0	0.0
3	3.0790970287424235E-4	2.3685361759557102E-5	0.0	0.0	0.0	0.0
4	3.0790970287424235E-4	2.3685361759557102E-5	0.0	0.0	0.0	0.0
5	3.0790970287424235E-4	2.3685361759557102E-5	2.3685361759557102E-5	0.0	0.0	0.0
6	3.3159506463379946E-4	2.3685361759557102E-5	2.3685361759557102E-5	0.0	0.0	0.0
7	3.3159506463379946E-4	2.3685361759557102E-5	2.3685361759557102E-5	0.0	0.0	0.0
8	3.3159506463379946E-4	4.7370723519114204E-5	2.3685361759557102E-5	7.105608527867131E-5	0.0	0.0
9	3.3159506463379946E-4	4.7370723519114204E-5	2.3685361759557102E-5	7.105608527867131E-5	0.0	0.0
10-14	3.694916434490908E-4	5.6844868222937047E-5	2.3685361759557102E-5	1.0895266409396267E-4	0.0	0.0
15-19	4.358106563758507E-4	7.105608527867131E-5	3.315950646337994E-5	1.7053460466881113E-4	4.73707235191142E-6	0.0
20-24	5.06866741654522E-4	9.000437468631698E-5	6.158194057484847E-5	2.2737947289174819E-4	4.263365116720278E-5	0.0
25-29	7.247720698424474E-4	9.474144703822841E-5	7.105608527867131E-5	2.463277622993938E-4	4.7370723519114204E-5	0.0
30-34	0.002761713181164358	9.474144703822841E-5	7.105608527867131E-5	2.6053897935512813E-4	6.63190129267599E-5	0.0
35-39	0.009019385758039344	9.474144703822841E-5	7.105608527867131E-5	3.26857992281888E-4	1.1368973644587409E-4	0.0
40-44	0.015731817280697828	9.947851939013982E-5	7.105608527867131E-5	4.0265114991247075E-4	2.036941111321911E-4	0.0
45-49	0.021700528444106217	1.231638811496969E-4	9.474144703822841E-5	4.263365116720278E-4	3.221209199299766E-4	0.0
50-54	0.027100790925285233	1.6579753231689973E-4	9.474144703822841E-5	4.7844430754305343E-4	4.358106563758507E-4	9.47414470382284E-6
55-59	0.03193734179658679	1.6579753231689973E-4	9.474144703822841E-5	5.352891757659905E-4	5.163408863583449E-4	4.737072351911421E-5
60-64	0.03726654819248715	1.7527167702072256E-4	1.4211217055734262E-4	5.826598992851047E-4	6.30030622804219E-4	7.105608527867131E-5
65-69	0.04169097376917241	1.8948289407645682E-4	1.6579753231689973E-4	7.10560852786713E-4	6.916125633790674E-4	7.105608527867131E-5
70-74	0.07419676424798857	1.9421996642836825E-4	1.847458217245454E-4	8.053022998249415E-4	7.295091421943586E-4	7.105608527867131E-5
75-79	0.548467711049008	2.131682558360139E-4	1.8948289407645682E-4	9.379403256784613E-4	8.431988786402328E-4	7.105608527867131E-5
80-84	0.7135641566578249	2.131682558360139E-4	1.9895703878027967E-4	0.0010563671344762469	9.710998321418411E-4	7.105608527867131E-5
85-89	0.7150894939551404	2.131682558360139E-4	2.2264240053983679E-4	0.001160582726218298	0.0011084749303472724	7.105608527867131E-5
90-94	0.7161553352343205	2.3685361759557102E-4	2.605389793551281E-4	0.001226901739145058	0.0011416344368106522	7.105608527867131E-5
95-99	0.7176238276634129	2.70013124058951E-4	2.936984858185081E-4	0.0012884836797199064	0.0011700568709221207	7.105608527867131E-5
100-104	0.7190923200925056	3.0790970287424235E-4	3.0790970287424235E-4	0.001364276837350489	0.001217427594441235	7.105608527867131E-5
105-109	0.7206508168962843	3.173838475780652E-4	3.2685799228188806E-4	0.001421121705573426	0.0012695353903122608	7.105608527867131E-5
110-114	0.722545645837049	3.600174987452679E-4	3.3159506463379946E-4	0.001430595850277249	0.0012790095350160835	7.105608527867131E-5
115-119	0.7246583801060015	3.8370286050482504E-4	3.3159506463379946E-4	0.0014448070673329834	0.0012932207520718179	7.105608527867131E-5
120-124	0.7337819814557828	4.0265114991247075E-4	3.505433540414451E-4	0.0014732295014444519	0.0013879621991100461	7.105608527867131E-5
125-129	0.7586658225203735	4.16862366968205E-4	3.742287158010022E-4	0.001511126080259743	0.001487440718500186	7.105608527867131E-5
130-134	0.7884193739627292	4.5002187343158497E-4	3.7896578815291363E-4	0.0016248158167056174	0.0015727080208345918	7.105608527867131E-5
135-139	0.822441027594157	4.594960181354078E-4	3.7896578815291363E-4	0.0017006089743362	0.001586919237890326	7.105608527867131E-5
140-144	0.8551836716905686	4.7370723519114203E-4	4.121252946162936E-4	0.0017195572637438456	0.001610604599649883	7.105608527867131E-5
145-149	0.884041916458413	4.879184522468763E-4	4.4528480107967357E-4	0.0017337684807995797	0.0016485011784651744	7.105608527867131E-5
150-154	0.9148565721075969	4.973925969506992E-4	4.5002187343158497E-4	0.0018000874937263399	0.001676923612576643	7.105608527867131E-5
155-159	0.9628383779601076	4.973925969506992E-4	4.7370723519114203E-4	0.001847458217245454	0.0016816606849285542	8.526730233440557E-5
160-164	1.0205074967722774	4.973925969506992E-4	4.8318137989496483E-4	0.0018664065066530996	0.0016816606849285542	9.474144703822841E-5
165-168	1.0488470321175876	4.973925969506992E-4	4.973925969506992E-4	0.0018948289407645681	0.0016875820253684436	9.474144703822841E-5
>>END_MODULE
