##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479018
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10448856
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.363251632523216	39.0	39.0	39.0	37.0	39.0
2	40.02657860343754	41.0	40.0	41.0	38.0	41.0
3	40.04636325737478	41.0	40.0	41.0	38.0	41.0
4	40.01860490756117	41.0	40.0	41.0	38.0	41.0
5	39.99815826727826	41.0	40.0	41.0	38.0	41.0
6	39.965476794780216	41.0	40.0	41.0	38.0	41.0
7	39.95593833430186	41.0	40.0	41.0	38.0	41.0
8	39.893817083898945	41.0	40.0	41.0	38.0	41.0
9	39.8393490158157	40.0	40.0	41.0	38.0	41.0
10-14	39.99586025494083	41.0	40.0	41.0	38.0	41.0
15-19	39.86781012198847	40.8	40.0	41.0	38.0	41.0
20-24	39.62819895307199	40.0	39.2	41.0	37.4	41.0
25-29	39.33079244273249	40.0	38.6	41.0	36.2	41.0
30-34	38.89904544574067	40.0	38.0	41.0	35.0	41.0
35-39	38.50299839523102	40.0	37.2	41.0	34.8	41.0
40-44	37.91865429095779	39.0	35.6	40.4	33.8	41.0
45-49	37.23037073149443	38.0	35.0	40.0	33.0	41.0
50-54	36.54784204127227	36.8	34.8	39.6	32.4	41.0
55-59	35.94564855712434	35.8	34.0	39.0	31.6	40.2
60-64	35.27920886267358	34.8	33.6	38.0	30.6	40.0
65-69	35.3961975550242	35.0	33.8	38.0	31.6	40.0
70-74	35.35824494088157	35.0	34.0	37.2	31.0	40.0
75-79	35.280912953532905	35.0	34.0	38.0	31.0	40.6
80-84	35.41342405331263	35.0	34.0	38.8	31.0	41.0
85-89	35.70633437765819	35.6	33.6	39.8	30.2	41.0
90-94	39.290601617594476	39.8	39.0	41.0	36.0	41.0
95-99	39.2182275828215	40.0	38.6	41.0	35.4	41.0
100-104	39.06850581936015	40.0	38.0	41.0	35.0	41.0
105-109	38.81441722448386	40.0	38.0	41.0	34.6	41.0
110-114	38.49423909722831	40.0	37.2	41.0	34.0	41.0
115-119	38.14458939629377	39.8	36.2	41.0	33.8	41.0
120-124	37.73863879306702	39.0	35.2	40.6	33.0	41.0
125-129	37.092237104301645	38.0	35.0	39.8	32.8	40.6
130-134	37.05675913203383	37.8	35.0	40.0	33.0	41.0
135-139	36.64485891462825	36.8	35.0	40.0	32.2	41.0
140-144	36.21453623082617	35.8	34.4	39.2	32.0	41.0
145-149	35.62411060851978	35.0	34.0	38.2	31.8	40.0
150-154	35.034635372472756	35.0	34.0	36.8	31.0	39.2
155-159	34.44701216110459	35.0	33.8	35.8	31.0	37.6
160-164	33.953526203889176	35.0	33.0	35.0	30.4	36.4
165-169	33.58820257845265	34.2	33.0	35.0	30.0	36.0
170-174	33.30826092008784	34.0	32.4	35.0	29.4	35.0
175-178	32.391754985654245	33.25	30.75	34.75	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	28.0
31	576.0
32	6331.0
33	44519.0
34	232897.0
35	964519.0
36	2626589.0
37	3750828.0
38	2483492.0
39	337671.0
40	1406.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.76589475441139	27.76434090009471	28.043567640323495	22.426196705170405
2	22.442007048427122	27.908701201356394	28.353840841523702	21.29545090869278
3	21.112186826959814	27.564625256583113	28.922304987263676	22.400882929193397
4	21.508268464987935	26.97859937968329	29.279444563117725	22.23368759221105
5	21.906417314967303	27.612171131461665	28.23036320913983	22.251048344431197
6	22.063286162619143	27.593145125169684	27.977005329578663	22.36656338263251
7	22.071966538729214	27.819552685959113	27.78191220168026	22.326568573631413
8	22.021090155707	28.063081738326183	27.836578473279754	22.079249632687063
9	22.28163542496901	27.937058372706066	27.566309651506348	22.214996550818576
10-14	22.08717968742224	27.86546967438349	27.80835337380475	22.238997264389518
15-19	22.17867295711607	27.721090232270402	27.753185611898566	22.347051198714958
20-24	22.15249210057063	27.70030518173473	27.761722431623138	22.38548028607151
25-29	22.16164908388057	27.681267690931904	27.671094328412604	22.48598889677492
30-34	22.14477642337113	27.760445736834733	27.66823277112825	22.42654506866589
35-39	22.09305592880216	27.70642834009771	27.756772607450998	22.443743123649135
40-44	22.167841149308597	27.748597549817895	27.664611322043292	22.41894997883022
45-49	22.41657842734171	27.572783087449952	27.53496459325308	22.475673891955253
50-54	22.331861019043618	27.670378460570227	27.5719485463289	22.425811974057254
55-59	22.24296516288482	27.623092901270724	27.631298584266066	22.502643351578396
60-64	22.17571952374499	27.678519064670816	27.66149901960559	22.484262391978604
65-69	22.0591249415247	27.64651938929965	27.82039488342073	22.47396078575492
70-74	21.990912689389155	27.672585400736693	27.86528592221005	22.471215987664102
75-79	21.9082433521909	27.601607295573793	27.99818468165319	22.491964670582117
80-84	21.80172260006263	27.637856239955838	28.10394171381058	22.45647944617095
85-89	21.700103819977993	27.50343578282637	28.390887959409145	22.405572437786493
90-94	21.841042356778647	27.698249162817802	28.169172588299723	22.29153589210383
95-99	22.042204130750793	27.912044107390223	27.726817582554048	22.318934179304936
100-104	22.08817982798603	27.82869161905706	27.721889558695395	22.361238994261516
105-109	22.080589223761176	27.82716527579641	27.739635822910152	22.35260967753226
110-114	22.113983086313986	27.77489834098434	27.711296234467405	22.39982233823427
115-119	22.102637019079598	27.846701736324757	27.701123193384795	22.349538051210846
120-124	21.987077092701945	27.95692711571029	27.78290099921894	22.27309479236882
125-129	21.98020075269883	27.98592537676819	27.83941006178165	22.194463808751326
130-134	21.971002477306893	28.0719387578818	27.882847875091834	22.074210889719474
135-139	21.90783976107423	28.150596260497863	27.98185694990224	21.959707028525667
140-144	21.803100483941943	28.289208526860993	28.106199249452583	21.80149173974448
145-149	21.63629287300955	28.425630772379627	28.269344170516558	21.66873218409427
150-154	21.46098853165274	28.64149748707546	28.435749320375177	21.461764660896623
155-159	21.2524017001797	28.784775233386974	28.682552269481327	21.280270796952006
160-164	21.036511441064984	29.05958773707648	28.892750693610044	21.01115012824849
165-169	20.66868279368227	29.288443999261403	29.302519678643996	20.740353528412328
170-174	20.34386960428783	29.62678458556742	29.687850815215512	20.341494994929242
175-178	20.062719582138083	29.65709629882072	30.420551069745017	19.859633049296185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	5.5
9	14.5
10	29.0
11	66.0
12	136.5
13	267.5
14	484.0
15	823.5
16	1454.0
17	2514.5
18	4142.5
19	6736.0
20	10573.0
21	16161.0
22	23703.5
23	33713.0
24	46499.5
25	61951.5
26	80685.0
27	101933.5
28	125698.5
29	152123.0
30	180018.0
31	208268.0
32	236526.0
33	264597.5
34	290341.5
35	313761.0
36	333892.5
37	349275.5
38	362101.5
39	370911.5
40	375505.0
41	378005.5
42	376067.5
43	370524.5
44	362576.0
45	351292.0
46	337626.0
47	323002.5
48	307657.0
49	291528.0
50	275894.0
51	260905.0
52	246418.0
53	233046.0
54	221038.0
55	209860.5
56	199434.0
57	189440.0
58	179862.5
59	170625.5
60	161199.0
61	151406.5
62	140371.5
63	128185.0
64	115102.5
65	101453.0
66	88217.0
67	74680.0
68	61658.0
69	49720.5
70	38886.0
71	29978.0
72	22396.0
73	16137.5
74	11332.5
75	7723.0
76	5093.5
77	3321.0
78	2114.0
79	1219.5
80	677.5
81	372.0
82	195.5
83	106.0
84	51.0
85	21.0
86	11.5
87	4.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3293.0
92-93	7572.0
94-95	12071.0
96-97	16928.0
98-99	21832.0
100-101	27142.0
102-103	33535.0
104-105	39567.0
106-107	46469.0
108-109	52715.0
110-111	57705.0
112-113	62303.0
114-115	68544.0
116-117	75196.0
118-119	82154.0
120-121	89532.0
122-123	97230.0
124-125	105218.0
126-127	113734.0
128-129	121985.0
130-131	129836.0
132-133	145843.0
134-135	205337.0
136-137	207837.0
138-139	206425.0
140-141	211798.0
142-143	218778.0
144-145	228796.0
146-147	235023.0
148-149	243129.0
150-151	251475.0
152-153	234257.0
154-155	237121.0
156-157	248972.0
158-159	262320.0
160-161	279519.0
162-163	296716.0
164-165	307155.0
166-167	324872.0
168-169	342989.0
170-171	363603.0
172-173	385375.0
174-175	430005.0
176-177	1409647.0
178-179	1907303.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.44776762482356
#Duplication Level	Percentage of total
1	91.62744026840491
2	6.627602097986775
3	1.0937640542816645
4	0.3812068791236839
5	0.15484302212829304
6	0.062438103022600486
7	0.02468309233269271
8	0.009300278290737572
9	0.0031148450377434336
>10	0.0021062674037354524
>50	0.0014382145964084003
>100	0.005361157070503679
>500	0.00670172032025738
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.570425700191486E-6	3.828170280076594E-5	9.570425700191486E-6	9.570425700191486E-6	0.0	0.0
2	5.742255420114891E-4	3.828170280076594E-5	9.570425700191486E-6	9.570425700191486E-6	0.0	0.0
3	5.837959677116806E-4	4.785212850095743E-5	1.914085140038297E-5	2.8711277100574454E-5	0.0	0.0
4	5.933663934118721E-4	5.742255420114891E-5	1.914085140038297E-5	3.828170280076594E-5	0.0	0.0
5	6.029368191120635E-4	6.699297990134039E-5	2.8711277100574454E-5	3.828170280076594E-5	0.0	0.0
6	6.029368191120635E-4	9.570425700191486E-5	2.8711277100574454E-5	3.828170280076594E-5	0.0	0.0
7	6.125072448122551E-4	9.570425700191486E-5	2.8711277100574454E-5	4.785212850095743E-5	0.0	0.0
8	6.125072448122551E-4	9.570425700191486E-5	3.828170280076594E-5	5.742255420114891E-5	0.0	0.0
9	6.220776705124465E-4	9.570425700191486E-5	4.785212850095743E-5	7.656340560153189E-5	0.0	0.0
10-14	6.316480962126381E-4	1.0527468270210634E-4	5.933663934118721E-5	1.1293102326225952E-4	0.0	0.0
15-19	6.45046692192906E-4	1.2058736382241271E-4	8.421974616168508E-5	1.263296192425276E-4	0.0	0.0
20-24	6.527030327530593E-4	1.4164230036283398E-4	1.0336059756206805E-4	1.4164230036283398E-4	1.1484510840229783E-5	0.0
25-29	6.833283949936721E-4	1.4738455578294888E-4	1.3972821522279568E-4	1.7800991802356162E-4	5.933663934118721E-5	0.0
30-34	7.369227789147443E-4	1.818380883036382E-4	1.7035357746340843E-4	2.1246345054425097E-4	1.1101693812222123E-4	0.0
35-39	8.881355049777699E-4	2.3734655736474885E-4	1.9523668428390628E-4	2.2203387624444246E-4	1.7226766260344673E-4	3.8281702800765945E-6
40-44	0.0010182932945003741	2.8902685614578286E-4	2.1820570596436587E-4	2.430888127848637E-4	2.2969021680459566E-4	1.914085140038297E-5
45-49	0.0011752482759835143	3.2348038866647223E-4	2.277761316645573E-4	2.6797191960536157E-4	2.7945643044559136E-4	1.914085140038297E-5
50-54	0.0013188046614863867	3.789888577275828E-4	2.526592384850552E-4	2.96683196705936E-4	3.4836349548697006E-4	1.914085140038297E-5
55-59	0.0014202511739084167	4.2109873080842534E-4	2.622296641852467E-4	3.5793392118716155E-4	4.6703677416934445E-4	2.488310682049786E-5
60-64	0.0014700173875494122	4.593804336091913E-4	2.6797191960536157E-4	3.86645198287736E-4	5.474283500509529E-4	2.8711277100574457E-5
65-69	0.0015255258566105228	4.7277902958945935E-4	2.7371417502547647E-4	4.115283051082338E-4	6.335621813526763E-4	3.253944738065105E-5
70-74	0.00262995298241262	5.072325621101487E-4	2.9668319670593605E-4	4.421536673488467E-4	7.101255869542081E-4	3.828170280076594E-5
75-79	0.02169424097719406	5.206311580904166E-4	3.1008179268620414E-4	4.6703677416934445E-4	8.115720993762379E-4	3.828170280076594E-5
80-84	0.0291132349799825	5.302015837906083E-4	3.2922264408658707E-4	4.8809171070976574E-4	9.99152443099991E-4	3.828170280076594E-5
85-89	0.029241478684365062	5.493424351909912E-4	3.636761766072765E-4	5.072325621101487E-4	0.0012422412558848548	3.828170280076594E-5
90-94	0.02931038574940644	5.589128608911827E-4	3.7324660230746794E-4	5.244593283704934E-4	0.0012709525329854293	3.828170280076594E-5
95-99	0.029342925196787096	5.991086488319868E-4	3.8090294286762113E-4	5.569987757511445E-4	0.0012939215546658887	3.828170280076594E-5
100-104	0.029354409707627323	6.373903516327529E-4	3.9047336856781257E-4	6.105931596722167E-4	0.0013149764912063103	3.828170280076594E-5
105-109	0.02938120689958786	6.412185219128295E-4	4.0961421996819555E-4	6.354762664927147E-4	0.001334117342606693	3.828170280076594E-5
110-114	0.02940609000640836	6.584452881731741E-4	4.172705605283487E-4	6.565312030331359E-4	0.0013743131305474971	3.828170280076594E-5
115-119	0.029430973113228853	6.89070650413787E-4	4.3641141192873173E-4	6.775861395735572E-4	0.0014317356847486462	3.828170280076594E-5
120-124	0.029675976011153755	7.0821150181417E-4	4.421536673488467E-4	7.196960126543996E-4	0.0014757596429695271	3.828170280076594E-5
125-129	0.030152583211023293	7.177819275143613E-4	4.498100079089998E-4	7.579777154551658E-4	0.0015178695160503696	3.828170280076594E-5
130-134	0.03070001156107425	7.254382680745145E-4	4.517240930490381E-4	8.058298439561232E-4	0.0015408385377308292	3.828170280076594E-5
135-139	0.03142927799942884	7.503213748950124E-4	4.593804336091913E-4	8.383692913367741E-4	0.00158486249595171	4.019578794080424E-5
140-144	0.03202264439284071	7.637199708752805E-4	4.7277902958945935E-4	8.63252398157272E-4	0.0016135737730522844	4.785212850095743E-5
145-149	0.03226956137590565	7.847749074157018E-4	4.861776255697274E-4	8.900495901178081E-4	0.0016269723690325525	4.785212850095743E-5
150-154	0.03246096988990948	8.077439290961613E-4	4.8809171070976574E-4	9.091904415181911E-4	0.0016537695609930886	4.785212850095743E-5
155-159	0.03294523343033917	8.287988656365828E-4	4.957480512699189E-4	9.589566551591868E-4	0.001713106200334276	4.785212850095743E-5
160-164	0.033814228083916556	8.613383130172337E-4	5.014903066900338E-4	9.800115916996082E-4	0.00175330198827508	4.785212850095743E-5
165-167	0.034370588831287685	8.740988806174889E-4	5.072325621101487E-4	9.85753847119723E-4	0.001767338612635361	5.104227040102126E-5
>>END_MODULE
