##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479019
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11841226
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.280802089243124	39.0	39.0	39.0	37.0	39.0
2	39.989485041498234	41.0	40.0	41.0	38.0	41.0
3	40.02152716281236	41.0	40.0	41.0	38.0	41.0
4	40.029538242070544	41.0	40.0	41.0	38.0	41.0
5	40.01669717307988	41.0	40.0	41.0	38.0	41.0
6	40.002324252573175	41.0	40.0	41.0	38.0	41.0
7	40.01829911868923	41.0	40.0	41.0	38.0	41.0
8	39.99360395621196	41.0	40.0	41.0	38.0	41.0
9	39.968335120028954	41.0	40.0	41.0	38.0	41.0
10-14	39.87278293649661	40.8	40.0	41.0	38.0	41.0
15-19	39.65306926833421	40.0	39.6	41.0	37.4	41.0
20-24	39.429795546508444	40.0	39.0	41.0	37.0	41.0
25-29	39.286048725022226	40.0	38.6	41.0	36.0	41.0
30-34	39.28998588490752	40.0	38.8	41.0	35.6	41.0
35-39	38.959320428475905	40.0	37.8	41.0	35.0	41.0
40-44	38.41971172579596	39.8	36.6	41.0	34.4	41.0
45-49	37.79313032282299	39.0	35.2	40.6	33.8	41.0
50-54	37.19379866578005	37.6	35.0	40.0	33.0	41.0
55-59	36.60657409967515	36.4	35.0	39.4	33.0	41.0
60-64	35.872255474222	35.4	34.4	38.6	32.6	40.4
65-69	35.58969892137858	35.0	34.0	37.0	32.8	40.0
70-74	35.38971336245082	35.0	34.0	36.8	33.0	40.0
75-79	35.20717844588052	35.0	34.0	36.0	32.2	40.0
80-84	35.16509190855744	35.0	34.0	36.0	32.0	40.0
85-89	35.068429316356266	35.0	33.8	36.2	31.4	40.0
90-94	39.36518829151239	40.6	38.8	40.6	36.8	41.0
95-99	39.508226380768605	40.8	39.0	41.0	36.0	41.0
100-104	39.29854969107412	40.0	38.8	41.0	35.4	41.0
105-109	39.040592238489616	40.0	38.0	41.0	35.0	41.0
110-114	38.740940539752856	40.0	38.0	41.0	34.8	41.0
115-119	38.50227766871389	40.0	37.0	41.0	34.0	41.0
120-124	38.54377975047807	40.0	37.0	41.0	34.0	41.0
125-129	37.92869425757332	39.4	35.6	40.6	33.6	40.8
130-134	37.854652598683074	39.0	35.0	40.8	33.8	41.0
135-139	37.45505245282281	38.4	35.0	40.8	33.0	41.0
140-144	36.914878329715556	37.0	35.0	40.0	33.0	41.0
145-149	36.305397789633396	35.8	35.0	39.0	33.0	41.0
150-154	35.68028285850863	35.0	34.6	37.4	33.0	39.6
155-159	35.103912895035435	35.0	34.0	36.4	32.2	38.4
160-164	34.62971828797739	35.0	34.0	35.6	32.0	37.0
165-169	34.296885057716324	35.0	34.0	35.0	32.0	36.0
170-174	34.08552814600668	35.0	34.0	35.0	31.0	36.0
175-178	33.75153650557223	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	110.0
31	1645.0
32	13702.0
33	73451.0
34	292035.0
35	957729.0
36	2361775.0
37	3645254.0
38	3490647.0
39	995204.0
40	9668.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.477100428621156	28.055828002944967	28.315404165075474	22.151667403358402
2	22.143458793878267	28.207509931826312	28.5863220582058	21.06270921608962
3	20.764935995647747	27.920284605664985	29.200844574708736	22.113934823978532
4	21.219753765361794	27.24095461061211	29.530320593492597	22.008971030533495
5	21.59112578376597	27.954748942381475	28.45536433474034	21.998760939112216
6	21.747976096394073	27.92152603117278	28.22531214250957	22.105185729923573
7	21.751362570058202	28.168316355080126	27.988529228307947	22.091791846553726
8	21.69441745305765	28.361843613152892	28.099902831007533	21.843836102781925
9	21.927121397733647	28.27333081895405	27.84844238257086	21.951105400741444
10-14	21.749063821600906	28.18502239548506	28.042792190605937	22.023121592308094
15-19	21.84350336696555	28.052900941169433	27.961006740349355	22.142588951515663
20-24	21.823436188110925	28.0202050024212	27.97693752319228	22.179421286275595
25-29	21.900924785997667	27.990262156975977	27.827061150593696	22.28175190643266
30-34	21.93144358531794	28.024650488049126	27.848543723428637	22.195362203204297
35-39	21.854990353194847	27.989530813785667	27.927547367139177	22.227931465880307
40-44	21.95858604505986	27.994131688728853	27.80228499988092	22.244997266330362
45-49	22.22453992517329	27.81619741063974	27.64968762525097	22.309575038936003
50-54	22.15263858657879	27.922105363076426	27.663329793722376	22.261926256622413
55-59	22.083280903514552	27.899144902732196	27.662937942405623	22.354636251347625
60-64	22.054952755736608	27.971218520784923	27.649337999291628	22.324490724186838
65-69	21.91982823400212	27.989824702273225	27.767830797250216	22.322516266474434
70-74	21.871353523697632	28.051363938159778	27.72753260515423	22.34974993298836
75-79	21.836125752519205	27.99843191912729	27.763836278439413	22.40160604991409
80-84	21.757191358394813	28.074731450949418	27.77126963035753	22.39680756029823
85-89	21.68079893078639	28.04486461114753	27.798599570686346	22.475736887379735
90-94	21.44754328970192	27.964162721388995	28.555240141336462	22.033053847572624
95-99	21.683205010166443	28.267007841822757	27.976782515460368	22.07300463255044
100-104	21.713242109956372	28.184178087281452	27.981043088714557	22.121536714047622
105-109	21.710949834614954	28.16526076396604	27.98673359957825	22.13705580184076
110-114	21.772064955719227	28.075490744863973	27.925504891256985	22.226939408159815
115-119	21.824122495890354	28.127275034645127	27.838543326780083	22.210059142684436
120-124	21.746588900249744	28.204677708489577	27.94013205420019	22.10860133706048
125-129	21.744353734048822	28.225265362957625	27.980792903972645	22.049587999020908
130-134	21.770700777425947	28.28923978399269	27.971021125366626	21.969038313214735
135-139	21.74872354069917	28.33685613741208	28.02725107894822	21.88716924294053
140-144	21.670779606555644	28.5015970847438	28.10722356762175	21.720399741078804
145-149	21.491931970375866	28.628380116652846	28.268495266889794	21.611192646081495
150-154	21.296959931310102	28.884723539106773	28.403125893782967	21.41519063580016
155-159	21.092233965022057	29.012181395696746	28.628644219901766	21.26694041937943
160-164	20.897966236390076	29.319770834386876	28.757140468882834	21.02512246034021
165-169	20.62181126272548	29.482239805303205	29.0214732055465	20.874475726424816
170-174	20.443819825627443	29.761708274738503	29.1938111372763	20.60066076235775
175-178	20.138881699269948	30.015672237070202	29.460092451932702	20.385353611727155
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	8.5
9	20.0
10	37.0
11	73.0
12	155.0
13	313.0
14	611.0
15	1052.0
16	1792.0
17	3085.5
18	5044.0
19	8148.5
20	12857.0
21	19265.0
22	28098.0
23	40117.5
24	55298.0
25	73728.0
26	95130.5
27	119820.5
28	147975.0
29	178325.5
30	210208.5
31	242627.5
32	274940.0
33	307010.0
34	336558.5
35	362958.5
36	386308.5
37	405100.0
38	419176.5
39	429148.5
40	434641.5
41	435564.5
42	433045.5
43	426911.0
44	416691.5
45	403788.0
46	388055.5
47	369820.5
48	351661.0
49	332583.5
50	312961.0
51	294827.0
52	277395.5
53	260978.0
54	245854.5
55	232568.5
56	220240.0
57	207849.5
58	196936.0
59	186254.0
60	174666.5
61	162107.5
62	149141.5
63	135537.0
64	120703.5
65	105730.0
66	90344.0
67	75429.0
68	61669.5
69	48785.0
70	37492.5
71	27964.0
72	20211.5
73	14146.0
74	9519.0
75	6274.5
76	4058.5
77	2513.5
78	1438.5
79	778.5
80	437.0
81	239.0
82	119.0
83	52.0
84	19.0
85	9.0
86	7.0
87	3.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2508.0
92-93	5707.0
94-95	8837.0
96-97	12427.0
98-99	16062.0
100-101	19568.0
102-103	23464.0
104-105	27200.0
106-107	31831.0
108-109	36027.0
110-111	39549.0
112-113	43331.0
114-115	47147.0
116-117	51149.0
118-119	55000.0
120-121	58649.0
122-123	62248.0
124-125	66519.0
126-127	70528.0
128-129	74694.0
130-131	79080.0
132-133	84667.0
134-135	189847.0
136-137	186741.0
138-139	189711.0
140-141	194176.0
142-143	196135.0
144-145	199950.0
146-147	209173.0
148-149	214103.0
150-151	226134.0
152-153	218850.0
154-155	212463.0
156-157	218767.0
158-159	222337.0
160-161	229800.0
162-163	231263.0
164-165	239425.0
166-167	245444.0
168-169	252638.0
170-171	255669.0
172-173	264157.0
174-175	275255.0
176-177	473758.0
178-179	5779238.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.40740157312403
#Duplication Level	Percentage of total
1	89.82548047976424
2	7.848852480747376
3	1.3484766066690919
4	0.5111160011957
5	0.25105173197611524
6	0.10717441226321162
7	0.04612615369245387
8	0.025733865505453007
9	0.013438043251556845
>10	0.009443880450009592
>50	0.001241439394577072
>100	0.005481338806442765
>500	0.0063835662837450876
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.445071481618542E-6	8.445071481618542E-6	0.0	8.445071481618542E-6	0.0	0.0
2	5.151493603787311E-4	8.445071481618542E-6	1.6890142963237083E-5	8.445071481618542E-6	8.445071481618542E-6	0.0
3	5.320395033419681E-4	1.6890142963237083E-5	1.6890142963237083E-5	8.445071481618542E-6	1.6890142963237083E-5	0.0
4	5.320395033419681E-4	3.3780285926474166E-5	2.5335214444855625E-5	2.5335214444855625E-5	1.6890142963237083E-5	0.0
5	5.320395033419681E-4	3.3780285926474166E-5	2.5335214444855625E-5	4.222535740809271E-5	1.6890142963237083E-5	0.0
6	5.320395033419681E-4	5.911550037132979E-5	3.3780285926474166E-5	4.222535740809271E-5	1.6890142963237083E-5	0.0
7	5.320395033419681E-4	6.756057185294833E-5	4.222535740809271E-5	5.067042888971125E-5	1.6890142963237083E-5	0.0
8	5.404845748235867E-4	6.756057185294833E-5	4.222535740809271E-5	5.067042888971125E-5	1.6890142963237083E-5	0.0
9	5.404845748235867E-4	6.756057185294833E-5	5.067042888971125E-5	5.911550037132979E-5	2.5335214444855625E-5	0.0
10-14	5.489296463052052E-4	7.938367192721429E-5	5.067042888971125E-5	7.431662903824316E-5	3.3780285926474166E-5	0.0
15-19	5.742648607500609E-4	1.0809691496471733E-4	6.756057185294835E-5	1.1823100074265958E-4	5.911550037132979E-5	0.0
20-24	5.911550037132979E-4	1.4863325807648633E-4	7.600564333456688E-5	1.5538931526178117E-4	9.458480059412767E-5	0.0
25-29	6.114231752691825E-4	1.891696011882553E-4	9.120677200148026E-5	1.9761467266987389E-4	1.2667607222427812E-4	0.0
30-34	6.452034611956565E-4	2.1450481563311096E-4	1.0978592926104103E-4	2.1112678704046355E-4	1.7227945822501827E-4	0.0
35-39	7.46544318975079E-4	2.5673017304120364E-4	1.249870579279544E-4	2.364620014853192E-4	2.3139495859634805E-4	1.6890142963237083E-6
40-44	8.208609480133223E-4	3.0571158763459123E-4	1.418772008911915E-4	2.499741158559088E-4	2.9388848756032525E-4	8.445071481618542E-6
45-49	9.03622648533184E-4	3.2260173059782827E-4	1.62145372447076E-4	2.7193130170811704E-4	3.3611384496841796E-4	8.445071481618542E-6
50-54	0.0010590119637949652	3.445589164500365E-4	1.824135440029605E-4	2.989555304492964E-4	3.8847328815445285E-4	4.222535740809271E-5
55-59	0.001158663807278064	3.732721594875395E-4	2.02681715558845E-4	3.3611384496841796E-4	4.543448457110776E-4	7.600564333456688E-5
60-64	0.0012211573362420412	4.0705244541401376E-4	2.1112678704046355E-4	3.5638201652430247E-4	5.016372460081414E-4	7.600564333456688E-5
65-69	0.0012920959366876368	4.34076674155193E-4	2.1450481563311096E-4	3.9016230245077666E-4	5.979110608985927E-4	7.600564333456688E-5
70-74	0.002114645898997283	4.644789314890198E-4	2.3139495859634805E-4	4.154975168956323E-4	6.739167042331596E-4	7.769465763089059E-5
75-79	0.016481401503526748	4.948811888228465E-4	2.5166313015223253E-4	4.4421075993313525E-4	7.161420616412523E-4	9.458480059412768E-5
80-84	0.022379439426289137	5.235944318603496E-4	2.7024228741179333E-4	5.083933031934363E-4	8.580192625324438E-4	1.0809691496471733E-4
85-89	0.02245882309821635	5.523076748978526E-4	2.7868735889341185E-4	5.590637320831474E-4	0.0011485297215001215	1.0978592926104103E-4
90-94	0.022502737469920767	5.708868321574134E-4	3.023335590419438E-4	5.996000751949165E-4	0.0011772429645376247	1.0978592926104103E-4
95-99	0.022533139727254594	5.979110608985928E-4	3.0740060193091493E-4	6.485814897883041E-4	0.0012194683219457175	1.0978592926104103E-4
100-104	0.02257029804177372	6.148012038618299E-4	3.276687734867994E-4	7.093860044559575E-4	0.0012431145220942494	1.0978592926104103E-4
105-109	0.022629413542145045	6.232462753434484E-4	3.4793694504268394E-4	7.397882617897842E-4	0.0012718277651317523	1.0978592926104103E-4
110-114	0.02268177298533108	6.570265612699226E-4	3.5807103082062617E-4	7.735685477162585E-4	0.0013005410081692555	1.0978592926104103E-4
115-119	0.022740888485702406	6.806727614184544E-4	3.631380737095973E-4	8.22549962309646E-4	0.0013241872083177874	1.0978592926104103E-4
120-124	0.02298748457296567	7.110750187522812E-4	3.7158314519121586E-4	8.411291195692068E-4	0.0013917477801707356	1.0978592926104103E-4
125-129	0.023404671104157625	7.245871331228709E-4	4.002963882287189E-4	8.782874340883283E-4	0.0014981556808391294	1.0978592926104103E-4
130-134	0.02388772919290621	7.41477276086108E-4	4.1380850259930857E-4	9.019336342368603E-4	0.00157585033847002	1.1485297215001217E-4
135-139	0.024470439125137883	7.634344619383163E-4	4.1380850259930857E-4	9.424699773486293E-4	0.0016315878102487024	1.1823100074265958E-4
140-144	0.024997411585590885	7.93836719272143E-4	4.17186531191956E-4	9.576711060155426E-4	0.0016653680961751765	1.1823100074265958E-4
145-149	0.02529974514463283	8.276170051986171E-4	4.290096312662219E-4	9.796282918677509E-4	0.0016805692248420899	1.1823100074265958E-4
150-154	0.025505804888784324	8.478851767545016E-4	4.306986455625456E-4	9.813173061640746E-4	0.0016923923249163558	1.1823100074265958E-4
155-159	0.025850363805234356	8.529522196434727E-4	4.3576568845151673E-4	0.0010235426635721672	0.0017177275393612113	1.2667607222427812E-4
160-164	0.026319909779612342	8.563302482361201E-4	4.4421075993313525E-4	0.0010573229494986415	0.0017531968395840091	1.2667607222427812E-4
165-167	0.02662449535771606	8.782874340883284E-4	4.475887885257827E-4	0.0010725240781655548	0.0017650199396582753	1.2667607222427812E-4
>>END_MODULE
