##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479020
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12074177
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.341022580669474	39.0	39.0	39.0	37.0	39.0
2	40.05907889208515	41.0	40.0	41.0	38.0	41.0
3	40.07955399361795	41.0	40.0	41.0	38.0	41.0
4	40.07624801259746	41.0	40.0	41.0	38.0	41.0
5	40.05972961966683	41.0	40.0	41.0	38.0	41.0
6	40.04260265523688	41.0	40.0	41.0	38.0	41.0
7	40.05516897756261	41.0	40.0	41.0	38.0	41.0
8	40.02753769470168	41.0	40.0	41.0	38.0	41.0
9	40.002846902111834	41.0	40.0	41.0	38.0	41.0
10-14	39.90451741762606	41.0	40.0	41.0	38.0	41.0
15-19	39.68858925954125	40.0	39.6	41.0	37.6	41.0
20-24	39.46027965301486	40.0	39.0	41.0	37.0	41.0
25-29	39.3019923759607	40.0	38.6	41.0	36.2	41.0
30-34	39.32262561663623	40.0	38.8	41.0	35.8	41.0
35-39	38.992653677347946	40.0	38.0	41.0	35.0	41.0
40-44	38.471612914072736	40.0	36.6	41.0	34.6	41.0
45-49	37.86148869608256	39.0	35.2	40.8	34.0	41.0
50-54	37.266132689623475	37.6	35.0	40.0	33.0	41.0
55-59	36.690392595702384	36.6	35.0	39.6	33.0	41.0
60-64	35.96497273478764	35.6	34.6	38.8	32.6	40.6
65-69	35.677390434147185	35.0	34.0	37.4	32.8	40.0
70-74	35.46347558098577	35.0	34.0	37.0	33.0	40.0
75-79	35.25803996413172	35.0	34.0	36.0	32.2	40.0
80-84	35.20204794082446	35.0	34.0	36.0	32.0	40.0
85-89	35.11205603495792	35.0	33.8	36.2	31.6	40.0
90-94	39.36729240055401	40.6	38.8	40.6	36.8	41.0
95-99	39.49827178350773	40.8	39.0	41.0	36.0	41.0
100-104	39.28565414827595	40.0	38.8	41.0	35.4	41.0
105-109	39.02484551996539	40.0	38.0	41.0	35.0	41.0
110-114	38.72448893298042	40.0	38.0	41.0	34.8	41.0
115-119	38.48462386362443	40.0	37.0	41.0	34.0	41.0
120-124	38.51514742776585	40.0	37.0	41.0	34.0	41.0
125-129	37.914313213717286	39.4	35.6	40.6	33.6	40.8
130-134	37.832469966456095	39.0	35.0	40.8	33.8	41.0
135-139	37.42188175389232	38.4	35.0	40.8	33.0	41.0
140-144	36.873987979418985	36.8	35.0	40.0	33.0	41.0
145-149	36.261594564002856	35.8	35.0	39.0	33.0	41.0
150-154	35.64001647631522	35.0	34.4	37.2	32.8	39.6
155-159	35.0633695770577	35.0	34.0	36.4	32.0	38.4
160-164	34.58237025220036	35.0	34.0	35.6	32.0	37.0
165-169	34.255952512701384	35.0	34.0	35.0	31.6	36.0
170-174	34.041312114430795	35.0	34.0	35.0	31.0	36.0
175-178	33.71112143491475	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	100.0
31	1490.0
32	12774.0
33	68761.0
34	281528.0
35	947795.0
36	2394040.0
37	3729468.0
38	3575326.0
39	1051378.0
40	11515.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.468005645436538	28.039393492409463	28.323305182622384	22.169295679531615
2	22.123280120872835	28.239655588948214	28.55283635480911	21.08422793536984
3	20.791263868336536	27.87145658043608	29.195281798502705	22.14199775272468
4	21.228146647179347	27.235545743614658	29.511725726730692	22.024581882475303
5	21.612032025039884	27.95330066802897	28.443752315375203	21.990914991555947
6	21.76668438768125	27.93901398000046	28.167932273976103	22.12636935834219
7	21.789576217078814	28.147044721971525	27.97027905090343	22.093100010046232
8	21.72493413008605	28.344697945044206	28.07177665194075	21.858591272928997
9	21.94064241397157	28.261371354751553	27.829731169254853	21.96825506202203
10-14	21.758635805984955	28.18086566065745	28.024147732801996	22.0363508005556
15-19	21.843772871641686	28.05087750494299	27.963548985574754	22.141800637840575
20-24	21.819868964982046	28.020919355414453	27.972495351028893	22.18671632857461
25-29	21.89656818845707	27.98457733392512	27.828515351398273	22.290339126219536
30-34	21.938928011408148	28.002847730325637	27.849911426675295	22.208312831590924
35-39	21.86411877182188	27.991769542553502	27.90777375551145	22.236337930113166
40-44	21.96796518719247	27.988711777208504	27.788784279044442	22.254538756554588
45-49	22.24885224061234	27.794813675499373	27.639878063738838	22.31645602014945
50-54	22.17574249574112	27.913223402307253	27.647941553283506	22.26309254866812
55-59	22.084547874360297	27.892302721750724	27.665605697183338	22.35754370670564
60-64	22.050215099546744	27.965669212899563	27.656453934707102	22.32766175284659
65-69	21.92734461321877	27.975173794454065	27.759180605021776	22.338300987305388
70-74	21.877098538475956	28.035654935321887	27.72510954576863	22.36213698043353
75-79	21.835596745020386	28.004962988367655	27.756389524519975	22.403050742091988
80-84	21.76047444061819	28.067665398643733	27.77010805788254	22.40175210285554
85-89	21.686461942706323	28.042419785630106	27.79396724099705	22.47715103066652
90-94	21.468110934096547	27.947913865144947	28.549563091848736	22.034412108909777
95-99	21.68622237628554	28.246590333840725	27.96775594352231	22.099431346351427
100-104	21.73489964664118	28.16832203442128	27.968339388142706	22.12843893079484
105-109	21.72802654611497	28.159502613643532	27.957458345045726	22.155012495195766
110-114	21.77404726813558	28.065511903257605	27.919161435037676	22.24127939356914
115-119	21.833401027169067	28.124666821403565	27.838533918027274	22.20339823340009
120-124	21.757443372032505	28.196328091908267	27.926697379139863	22.119531156919365
125-129	21.74174653141813	28.215008426061516	27.978408896791386	22.064836145728968
130-134	21.78183281463326	28.283220573769206	27.975753162768918	21.959193448828618
135-139	21.754885367918778	28.333602346739028	28.03907798605528	21.872434299286915
140-144	21.656521335129238	28.506029048626853	28.115476968227565	21.721972648016347
145-149	21.49619380198588	28.6286670733931	28.274755032831123	21.6003840917899
150-154	21.305713419551687	28.87401624781573	28.414269774827332	21.40600055780525
155-159	21.106454462132458	29.002580637964236	28.625130701755314	21.26583419814799
160-164	20.905578375728325	29.309837287259725	28.77555255205265	21.0090317849593
165-169	20.62262613090556	29.484763018025905	29.039172240163897	20.85343861090464
170-174	20.429160094732154	29.770701872624077	29.215456344446057	20.584681688197715
175-178	20.110545419424785	30.020421267304638	29.480831421171988	20.38820189209859
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	3.0
8	6.5
9	15.0
10	40.0
11	92.0
12	175.0
13	340.0
14	633.0
15	1054.5
16	1783.5
17	3088.5
18	5060.5
19	8213.0
20	12888.0
21	19274.5
22	28286.5
23	40539.5
24	56020.5
25	74693.5
26	96541.0
27	121781.0
28	150251.5
29	181173.0
30	213700.5
31	246477.0
32	278851.0
33	311311.5
34	342174.0
35	369420.5
36	393136.5
37	411717.0
38	426525.0
39	437250.5
40	443195.5
41	445249.0
42	442555.5
43	435734.5
44	425977.0
45	413246.5
46	397431.0
47	379002.0
48	359306.5
49	339783.0
50	320370.0
51	302071.5
52	284219.0
53	267030.5
54	251726.0
55	238007.0
56	225129.5
57	212737.0
58	200604.0
59	189715.0
60	178462.0
61	165200.0
62	151741.5
63	137635.5
64	122618.0
65	107347.0
66	91902.0
67	76618.0
68	62068.0
69	49154.0
70	37890.0
71	28387.5
72	20639.5
73	14414.0
74	9879.5
75	6584.0
76	4156.5
77	2554.5
78	1518.5
79	845.0
80	463.5
81	253.5
82	126.0
83	58.5
84	25.0
85	8.5
86	4.0
87	2.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2550.0
92-93	5782.0
94-95	9553.0
96-97	13063.0
98-99	16879.0
100-101	21115.0
102-103	24966.0
104-105	29274.0
106-107	34399.0
108-109	38693.0
110-111	42597.0
112-113	46385.0
114-115	50227.0
116-117	54415.0
118-119	58343.0
120-121	62317.0
122-123	66679.0
124-125	70643.0
126-127	75004.0
128-129	79356.0
130-131	83773.0
132-133	89568.0
134-135	189584.0
136-137	192444.0
138-139	195027.0
140-141	205373.0
142-143	206230.0
144-145	209686.0
146-147	213077.0
148-149	222007.0
150-151	232943.0
152-153	222305.0
154-155	219309.0
156-157	224583.0
158-159	232942.0
160-161	242068.0
162-163	243121.0
164-165	246159.0
166-167	252596.0
168-169	265182.0
170-171	271133.0
172-173	275144.0
174-175	288276.0
176-177	476887.0
178-179	5772520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.12764159260298
#Duplication Level	Percentage of total
1	89.27496045835046
2	8.338987622225728
3	1.4530945727827098
4	0.5029198464403093
5	0.21440556644490347
6	0.1050992197451481
7	0.05840408702007309
8	0.016462353894969875
9	0.011364900925973318
>10	0.012636259966453466
>50	0.001376555507628098
>100	0.0038234511179069328
>500	0.0064651055777406215
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.1410689937707556E-5	8.282137987541511E-6	8.282137987541511E-6	0.0	8.282137987541511E-6	0.0
2	5.300568312026567E-4	1.6564275975083023E-5	8.282137987541511E-6	1.6564275975083023E-5	8.282137987541511E-6	0.0
3	5.300568312026567E-4	3.3128551950166045E-5	8.282137987541511E-6	2.4846413962624534E-5	1.6564275975083023E-5	0.0
4	5.383389691901982E-4	3.3128551950166045E-5	8.282137987541511E-6	4.1410689937707556E-5	1.6564275975083023E-5	0.0
5	5.466211071777397E-4	5.797496591279058E-5	8.282137987541511E-6	4.969282792524907E-5	1.6564275975083023E-5	0.0
6	5.549032451652812E-4	6.625710390033209E-5	8.282137987541511E-6	7.45392418878736E-5	2.4846413962624534E-5	0.0
7	5.549032451652812E-4	6.625710390033209E-5	1.6564275975083023E-5	7.45392418878736E-5	3.3128551950166045E-5	0.0
8	5.631853831528227E-4	8.282137987541511E-5	1.6564275975083023E-5	8.282137987541511E-5	3.3128551950166045E-5	0.0
9	5.631853831528227E-4	8.282137987541511E-5	1.6564275975083023E-5	9.110351786295662E-5	3.3128551950166045E-5	0.0
10-14	5.913446523104638E-4	9.110351786295662E-5	1.8220703572591326E-5	1.1098064903305625E-4	5.134925552275737E-5	0.0
15-19	6.377246250406964E-4	1.0932422143554794E-4	2.6502841560132837E-5	1.308577802031559E-4	6.625710390033209E-5	0.0
20-24	6.708531769908625E-4	1.4576562858073058E-4	5.466211071777397E-5	1.4576562858073058E-4	1.0104208344800644E-4	0.0
25-29	6.940431633559786E-4	1.7061204254335512E-4	5.9631393510298876E-5	1.8220703572591323E-4	1.490784837757472E-4	0.0
30-34	7.387667084887028E-4	1.8551989092092987E-4	7.45392418878736E-5	2.252741532611291E-4	1.8551989092092987E-4	0.0
35-39	9.143480338245828E-4	2.087098772860461E-4	1.1760635942308947E-4	2.766234087838865E-4	2.5012056722375364E-4	3.3128551950166047E-6
40-44	0.0010435493864302304	2.203048704686042E-4	1.2754492500813928E-4	3.1969052631910237E-4	3.3294194709916874E-4	1.3251420780066419E-5
45-49	0.0011164322007205956	2.236177256636208E-4	1.557041941657804E-4	3.3294194709916874E-4	4.091376165845506E-4	3.975426234019925E-5
50-54	0.0012356949877411934	2.3355629124867063E-4	1.7392489773837173E-4	3.760090646343846E-4	4.6214329970481634E-4	4.969282792524907E-5
55-59	0.0013152035124215917	2.534334224187702E-4	1.8220703572591323E-4	3.991990509995009E-4	5.184618380200986E-4	7.61956694853819E-5
60-64	0.0013996813198945154	2.617155604063118E-4	1.8220703572591323E-4	4.3729688574219177E-4	5.81406086725414E-4	8.779066266794001E-5
65-69	0.0014377791546372062	2.8490554677142795E-4	1.8386346332342155E-4	4.6048687210730805E-4	6.62571039003321E-4	1.0932422143554794E-4
70-74	0.0022494286774162745	3.3459837469667703E-4	1.97114884103488E-4	4.8036400327740764E-4	7.139202945260783E-4	1.1926278702059775E-4
75-79	0.01693365932932737	3.826347750244178E-4	2.087098772860461E-4	5.068668448375406E-4	8.083366675840515E-4	1.4079634578820568E-4
80-84	0.023069067150498123	4.008554785970091E-4	2.318998636511623E-4	5.681546659453477E-4	9.640408617498318E-4	1.4079634578820568E-4
85-89	0.023136980681995965	4.1410689937707555E-4	2.451512844312287E-4	6.045960730905304E-4	0.0013234856504091333	1.4079634578820568E-4
90-94	0.023160170668361083	4.356404581446835E-4	2.766234087838865E-4	6.310989146506632E-4	0.0013450192091767415	1.4079634578820568E-4
95-99	0.02318998636511623	4.82020430874916E-4	2.882184019664446E-4	6.576017562107959E-4	0.0013582706299568078	1.4079634578820568E-4
100-104	0.023257899896614073	4.969282792524907E-4	3.031262503440193E-4	6.741660321858791E-4	0.0013748349059318908	1.4079634578820568E-4
105-109	0.023309249152136828	5.416518243852148E-4	3.180340987215941E-4	7.023253013435202E-4	0.0013963684646994988	1.4079634578820568E-4
110-114	0.023345690559282012	5.698110935428559E-4	3.279726643066438E-4	7.387667084887029E-4	0.001446061292624748	1.474220561782389E-4
115-119	0.02336225483525709	6.178474938705967E-4	3.3956765748920195E-4	7.718952604388689E-4	0.001474220561782389	1.490784837757472E-4
120-124	0.02357593399533566	6.344117698456797E-4	3.544755058667767E-4	8.033673847915266E-4	0.001555385514060296	1.5901704936079698E-4
125-129	0.024018200163870386	6.758224597833873E-4	3.710397818418597E-4	8.398087919367092E-4	0.0016183297627656115	1.6729918734833852E-4
130-134	0.024457153477210083	6.923867357584703E-4	3.72696209439368E-4	8.547166403142841E-4	0.0016547711699107937	1.7392489773837173E-4
135-139	0.024906045356134835	7.437359912812277E-4	3.8594763021943446E-4	8.71280916289367E-4	0.0016978382874460098	1.7392489773837173E-4
140-144	0.025419537911362405	7.735516880363771E-4	3.9754262340199254E-4	8.895016198619583E-4	0.0017723775293338833	1.7392489773837173E-4
145-149	0.02574585414807154	7.801773984264104E-4	4.1079404418205886E-4	9.126916062270746E-4	0.0018253832124541492	1.7392489773837173E-4
150-154	0.02592971761139496	7.983981019990016E-4	4.190761821696004E-4	9.342251649946825E-4	0.0018485731988192654	1.7392489773837173E-4
155-159	0.02624609528251905	8.215880883641179E-4	4.306711753521585E-4	9.54102296164782E-4	0.0018833581783669396	1.7392489773837173E-4
160-164	0.026761244265344132	8.49747357521759E-4	4.389533133397001E-4	9.656972893473401E-4	0.0019115174475245807	1.7392489773837173E-4
165-167	0.02702461625334795	8.61342350704317E-4	4.4171402600221395E-4	9.855744205174398E-4	0.001932498863759686	1.7392489773837173E-4
>>END_MODULE
