##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479059
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6388662
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29516383868798	39.0	39.0	39.0	37.0	39.0
2	40.02801572535846	41.0	40.0	41.0	38.0	41.0
3	40.048546002277156	41.0	40.0	41.0	38.0	41.0
4	40.06589861852137	41.0	40.0	41.0	38.0	41.0
5	40.07498095845421	41.0	40.0	41.0	38.0	41.0
6	40.05850849520604	41.0	40.0	41.0	38.0	41.0
7	40.073661276805694	41.0	40.0	41.0	38.0	41.0
8	40.057232328146334	41.0	40.0	41.0	38.0	41.0
9	40.03168863840347	41.0	40.0	41.0	38.0	41.0
10-14	39.95462624255283	41.0	40.0	41.0	38.0	41.0
15-19	39.80779061405972	41.0	40.0	41.0	37.8	41.0
20-24	39.58772969989648	40.2	39.2	41.0	36.8	41.0
25-29	39.28050280324737	40.0	38.6	41.0	35.8	41.0
30-34	39.0955169016611	40.0	38.0	41.0	35.0	41.0
35-39	38.80449433699889	40.0	37.2	41.0	35.0	41.0
40-44	38.17229898842668	39.2	35.4	41.0	35.0	41.0
45-49	37.482344378212524	37.8	35.0	40.6	34.0	41.0
50-54	36.85020015145581	36.4	35.0	39.6	34.0	41.0
55-59	36.282993684749634	35.2	35.0	38.8	33.2	41.0
60-64	35.69205069856568	35.0	34.8	37.0	32.8	39.8
65-69	35.42500507931082	35.0	34.8	36.0	33.0	39.0
70-74	35.23582302522813	35.0	35.0	36.0	33.0	38.6
75-79	35.049417358439065	35.0	35.0	35.2	33.0	37.2
80-84	34.96490967905329	35.0	35.0	35.0	33.0	37.0
85-89	34.78054634914165	35.0	33.8	35.0	32.4	37.8
90-94	39.359567937449754	40.6	39.8	40.6	36.8	40.6
95-99	39.58809991296798	41.0	39.0	41.0	36.4	41.0
100-104	39.44865052362937	40.8	39.0	41.0	36.0	41.0
105-109	39.27200887784485	40.0	38.8	41.0	35.0	41.0
110-114	39.038231021868235	40.0	38.0	41.0	35.0	41.0
115-119	38.71034251285065	40.0	37.8	41.0	34.6	41.0
120-124	38.311956069670906	40.0	36.6	41.0	34.0	41.0
125-129	37.75403673159024	39.0	35.4	40.6	33.6	40.8
130-134	37.41943910958063	38.4	35.0	40.2	33.0	41.0
135-139	37.03781119842726	37.0	35.0	40.0	33.0	41.0
140-144	36.62914402688814	35.8	35.0	39.4	33.0	41.0
145-149	36.080156279330446	35.0	35.0	38.4	33.0	41.0
150-154	35.58520703321973	35.0	35.0	36.8	33.0	39.4
155-159	35.16218104627348	35.0	35.0	36.0	33.0	38.2
160-164	34.8256868089487	35.0	35.0	35.0	33.0	36.8
165-169	34.57248108437087	35.0	34.0	35.0	33.0	36.0
170-174	34.41546538448006	35.0	34.0	35.0	33.0	36.0
175-178	34.095602077495755	34.75	33.75	35.0	32.25	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	58.0
30	468.0
31	3471.0
32	18460.0
33	68713.0
34	208222.0
35	558614.0
36	1397827.0
37	2021773.0
38	1506605.0
39	588625.0
40	15823.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.827089929002348	26.658430200251633	28.94798629196536	22.56649357878066
2	23.47308403543653	27.57892028096024	27.630245581938755	21.31775010166448
3	20.190362238603328	26.2031235961458	30.394220260830828	23.21229390442005
4	21.611536187076418	24.832132299376614	30.61608205286177	22.940249460685195
5	23.0381885909757	25.888331547356863	27.7813413825931	23.292138479074335
6	22.86891997103619	26.507929203329272	27.68432576336015	22.938825062274386
7	22.91338937636707	26.54590272579767	27.1335375075407	23.407170390294556
8	23.283748615907367	26.617467006393515	26.621521063408892	23.477263314290223
9	23.292435880940328	26.886271335062023	26.79237060905711	23.028922174940543
10-14	23.316763353578573	26.685490639511062	26.590034032165104	23.40771197474526
15-19	23.29574173747179	26.637493108885714	26.71142721277163	23.355337940870875
20-24	23.357047218963846	26.414335270828225	26.71921914166065	23.50939836854728
25-29	23.421786909371633	26.365623975724496	26.463284487424755	23.749304627479116
30-34	23.54277938009555	26.416479694809336	26.441198485692308	23.599542439402803
35-39	23.546639343261546	26.389472474831194	26.446176053765246	23.617712128142013
40-44	23.574545029929585	26.317939499694926	26.384350901644193	23.723164568731292
45-49	23.689329628019138	26.30358281593235	26.346571473025183	23.66051608302333
50-54	23.713613273013973	26.361194253194174	26.283625585451226	23.641566888340627
55-59	23.644158980393705	26.314499029687283	26.263646441148396	23.77769554877062
60-64	23.597185138296563	26.380775818160362	26.36717046542766	23.65486857811542
65-69	23.625316224273565	26.365173177106566	26.339017465628956	23.670493132990913
70-74	23.55962171734864	26.36358912085191	26.30408683383156	23.77270232796789
75-79	23.539367085001523	26.387807024381633	26.376740544420723	23.69608534619612
80-84	23.543117479058996	26.418508288590004	26.32154275809238	23.716831474258615
85-89	23.5078456177522	26.368112759760965	26.271698205351917	23.852343417134918
90-94	22.01940575777254	26.465619696071947	28.733606809168784	22.78136773698673
95-99	22.879137984659664	26.834888312046022	26.9774385231245	23.308535180169816
100-104	23.145146967060708	26.824559004512512	26.631050671847174	23.399243356579607
105-109	23.099699701518755	26.72496212826768	26.688024254061904	23.487313916151656
110-114	23.135708118859004	26.574997586282244	26.72606810734923	23.56322618750951
115-119	23.2812818404879	26.61652737616163	26.457099617773096	23.645091165577377
120-124	23.258672008387286	26.582904996891337	26.416721578853153	23.74170141586822
125-129	23.24696525415639	26.595660797693544	26.493372094007757	23.664001854142313
130-134	23.4240797229884	26.574380519846137	26.33348103053389	23.66805872663158
135-139	23.52731235533788	26.500246476879692	26.26932916127324	23.703112006509187
140-144	23.454990615406746	26.634634142951775	26.391382498845918	23.51899274279556
145-149	23.429292734272657	26.69021406465416	26.382152888614304	23.498340312458883
150-154	23.302958789610646	26.761059930622377	26.4519262928279	23.484054986939086
155-159	23.200743157977158	26.850104310742033	26.61010087073764	23.33905166054317
160-164	23.140796461592604	27.00247944460441	26.59576654829318	23.260957545509804
165-169	23.005366744219423	27.04307346355751	26.71075460275899	23.24080518946408
170-174	22.866985952012104	27.254792284831325	26.871942878493105	23.00627888466347
175-178	22.79245598902192	27.34876680327733	26.837276454812454	23.021500752888297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	9.0
8	23.5
9	44.0
10	101.5
11	219.5
12	424.5
13	724.5
14	1203.0
15	1970.5
16	3098.5
17	4746.0
18	7040.0
19	10175.5
20	14155.0
21	18846.5
22	24336.0
23	30756.0
24	37661.5
25	44693.5
26	52454.0
27	60631.0
28	68879.5
29	77292.0
30	85796.0
31	94178.5
32	102234.5
33	110535.0
34	118977.0
35	127582.5
36	136320.5
37	145134.5
38	153724.0
39	161779.0
40	170225.5
41	178010.0
42	184202.0
43	189343.0
44	193596.5
45	196092.5
46	196959.5
47	196663.0
48	194575.0
49	190845.0
50	186807.5
51	182946.5
52	178792.5
53	174630.5
54	170941.5
55	167843.0
56	165318.0
57	162207.0
58	159298.5
59	155594.0
60	149443.5
61	142445.0
62	133502.5
63	122558.5
64	109973.5
65	95845.0
66	81266.0
67	67031.5
68	53818.0
69	41771.0
70	31186.5
71	22843.0
72	16292.0
73	11202.5
74	7578.0
75	4867.0
76	3009.5
77	1847.0
78	1074.5
79	618.5
80	346.5
81	182.0
82	101.5
83	59.5
84	28.5
85	7.0
86	3.0
87	2.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1331.0
92-93	2621.0
94-95	3794.0
96-97	5015.0
98-99	6133.0
100-101	7118.0
102-103	8425.0
104-105	9555.0
106-107	10516.0
108-109	11620.0
110-111	12739.0
112-113	12987.0
114-115	15126.0
116-117	14722.0
118-119	15790.0
120-121	16951.0
122-123	16928.0
124-125	17719.0
126-127	20774.0
128-129	19358.0
130-131	21972.0
132-133	22565.0
134-135	98725.0
136-137	81139.0
138-139	76510.0
140-141	73404.0
142-143	73185.0
144-145	78394.0
146-147	79267.0
148-149	80483.0
150-151	80316.0
152-153	82735.0
154-155	77523.0
156-157	75916.0
158-159	76659.0
160-161	79092.0
162-163	81170.0
164-165	83826.0
166-167	87625.0
168-169	96591.0
170-171	91992.0
172-173	94758.0
174-175	101965.0
176-177	228617.0
178-179	4135011.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.35525448350181
#Duplication Level	Percentage of total
1	92.96397659934861
2	6.350824252259661
3	0.5766340670014816
4	0.0675295456126423
5	0.020623804989480934
6	0.011431714368746342
7	0.0010473444561975864
8	0.001055483322341407
9	0.001063663343838436
>10	0.0058135252969892205
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2522183831293625E-4	0.0	0.0	1.565272978911703E-5	0.0	0.0
2	2.6609640641498956E-4	0.0	0.0	3.130545957823406E-5	1.565272978911703E-5	0.0
3	2.8174913620410656E-4	4.69581893673511E-5	0.0	3.130545957823406E-5	1.565272978911703E-5	0.0
4	2.8174913620410656E-4	4.69581893673511E-5	0.0	3.130545957823406E-5	1.565272978911703E-5	0.0
5	2.974018659932236E-4	4.69581893673511E-5	0.0	3.130545957823406E-5	1.565272978911703E-5	0.0
6	3.287073255714577E-4	6.261091915646813E-5	0.0	3.130545957823406E-5	1.565272978911703E-5	0.0
7	3.287073255714577E-4	6.261091915646813E-5	0.0	3.130545957823406E-5	3.130545957823406E-5	0.0
8	3.4436005536057473E-4	7.826364894558517E-5	0.0	4.69581893673511E-5	3.130545957823406E-5	0.0
9	3.4436005536057473E-4	7.826364894558517E-5	0.0	4.69581893673511E-5	3.130545957823406E-5	0.0
10-14	3.85057152812279E-4	9.07858327768788E-5	3.1305459578234063E-6	1.00177470650349E-4	4.069709745170429E-5	0.0
15-19	4.00709882601396E-4	1.00177470650349E-4	4.69581893673511E-5	1.6278838980681717E-4	4.69581893673511E-5	0.0
20-24	4.163626123905131E-4	1.0956910852381921E-4	9.07858327768788E-5	2.0974657917416823E-4	6.574146511429153E-5	0.0
25-29	4.758429855891578E-4	1.1896074639728945E-4	1.00177470650349E-4	2.410520387524023E-4	1.0956910852381921E-4	0.0
30-34	5.196706289986855E-4	2.3166040087893206E-4	1.2522183831293625E-4	2.880102281197534E-4	1.3148293022858306E-4	0.0
35-39	6.448924673116217E-4	2.8174913620410656E-4	1.3148293022858306E-4	3.224462336558109E-4	1.5965784384899375E-4	3.1305459578234063E-6
40-44	6.824590188055027E-4	2.9427132003540023E-4	1.6278838980681717E-4	3.4436005536057473E-4	2.0974657917416823E-4	1.565272978911703E-5
45-49	7.482004839197941E-4	3.474906013183982E-4	1.878327574694044E-4	3.506211472762215E-4	3.193156876979875E-4	1.565272978911703E-5
50-54	8.671612303170836E-4	4.320153421796301E-4	2.1287712513199164E-4	3.85057152812279E-4	4.0384042855921945E-4	2.1913821704763844E-5
55-59	8.922055979796709E-4	4.5392916388439395E-4	2.410520387524023E-4	4.0384042855921945E-4	4.132320664326897E-4	3.7566551493880876E-5
60-64	9.01597235853141E-4	4.601902558000408E-4	2.8174913620410656E-4	4.1949315834833645E-4	4.946262613360981E-4	8.139419490340858E-5
65-69	9.329026954313752E-4	4.883651694204513E-4	2.9427132003540023E-4	4.5392916388439395E-4	5.321928128299792E-4	1.1896074639728945E-4
70-74	0.001177085280141601	5.54106634534743E-4	3.00532411951047E-4	4.6958189367351096E-4	5.760204562395069E-4	1.5652729789117034E-4
75-79	0.004661382931199052	6.135870077333877E-4	3.224462336558109E-4	5.040178992095684E-4	6.417619213537984E-4	1.7218002768028736E-4
80-84	0.006013778784978764	6.730673809320324E-4	3.787960608966322E-4	5.290622668721556E-4	8.295946788232026E-4	1.815716655537576E-4
85-89	0.006088911887966525	6.730673809320324E-4	4.101015204748663E-4	5.321928128299791E-4	0.0012490878371715392	1.878327574694044E-4
90-94	0.006117086801586937	6.855895647633261E-4	4.2262370430615995E-4	5.447149966612728E-4	0.0012678711129184797	1.878327574694044E-4
95-99	0.006126478439460407	6.949812026367962E-4	4.2262370430615995E-4	5.885426400708005E-4	0.001280393296749773	1.878327574694044E-4
100-104	0.006173436628827758	7.075033864680899E-4	4.2262370430615995E-4	6.22978645606858E-4	0.0012835238427075966	1.878327574694044E-4
105-109	0.006207872634363816	7.294172081728537E-4	4.2262370430615995E-4	6.355008294381514E-4	0.0012960460265388901	1.878327574694044E-4
110-114	0.006232917002026403	7.732448515823814E-4	4.476680719687471E-4	6.511535592272686E-4	0.001308568210370184	1.878327574694044E-4
115-119	0.00625796136968899	8.358557707388496E-4	4.5392916388439395E-4	6.761979268898558E-4	0.001386831859315769	1.878327574694044E-4
120-124	0.00627674464543593	8.452474086123199E-4	4.6958189367351096E-4	7.168950243415601E-4	0.0014431816865565904	1.9096330342722778E-4
125-129	0.006345616656508045	8.609001384014367E-4	4.6958189367351096E-4	7.356783000885006E-4	0.0015277064274178223	2.0661603321634484E-4
130-134	0.006414488667580159	9.047277818109645E-4	4.6958189367351096E-4	7.638532137089112E-4	0.001602839530405584	2.1913821704763842E-4
135-139	0.006561624327597861	9.360332413891985E-4	4.6958189367351096E-4	7.669837596667346E-4	0.001646667173815112	2.1913821704763842E-4
140-144	0.006686846165910797	9.735997928830793E-4	4.6958189367351096E-4	7.669837596667346E-4	0.0017437140985076375	2.1913821704763842E-4
145-149	0.0068026763663502614	0.0010049052524613133	4.883651694204515E-4	7.669837596667346E-4	0.0018376304772423397	2.4105203875240226E-4
150-154	0.006934159296578845	0.0010268190741660774	5.134095370830387E-4	7.826364894558517E-4	0.0019346774019348655	2.6609640641498956E-4
155-159	0.007037467313187018	0.0010581245337443115	5.165400830408621E-4	7.826364894558517E-4	0.00200354941300698	2.8174913620410656E-4
160-164	0.00715016696766866	0.0010800383554490753	5.165400830408621E-4	8.045503111606154E-4	0.002025463234711744	2.8174913620410656E-4
165-167	0.007215908432782952	0.00109047350864182	5.165400830408621E-4	8.139419490340857E-4	0.0020661603321634483	2.869667128004789E-4
>>END_MODULE
