##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479060
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6314888
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.189262105677884	39.0	39.0	39.0	37.0	39.0
2	39.9524306686041	41.0	40.0	41.0	38.0	41.0
3	40.00843403715157	41.0	40.0	41.0	38.0	41.0
4	40.02857390344849	41.0	40.0	41.0	38.0	41.0
5	40.04614713673465	41.0	40.0	41.0	38.0	41.0
6	40.03307279559036	41.0	40.0	41.0	38.0	41.0
7	40.048113759103884	41.0	40.0	41.0	38.0	41.0
8	40.03052437351224	41.0	40.0	41.0	38.0	41.0
9	40.00430316420497	41.0	40.0	41.0	38.0	41.0
10-14	39.9314274140729	41.0	40.0	41.0	38.0	41.0
15-19	39.77973161202542	41.0	40.0	41.0	37.6	41.0
20-24	39.55870849965986	40.2	39.0	41.0	36.8	41.0
25-29	39.252040701276094	40.0	38.4	41.0	35.6	41.0
30-34	39.05611497781116	40.0	38.0	41.0	35.0	41.0
35-39	38.75907379513303	40.0	37.2	41.0	35.0	41.0
40-44	38.12756520780732	39.2	35.4	41.0	34.8	41.0
45-49	37.451818527897885	37.8	35.0	40.4	34.0	41.0
50-54	36.825876943502394	36.4	35.0	39.6	34.0	41.0
55-59	36.277895981686456	35.2	35.0	38.8	33.0	41.0
60-64	35.714722034658415	35.0	34.8	37.0	32.8	39.8
65-69	35.460566964924794	35.0	34.8	36.4	33.0	39.0
70-74	35.265230040501116	35.0	35.0	36.0	33.0	38.8
75-79	35.08476888267852	35.0	35.0	35.2	33.0	37.8
80-84	34.98859298850589	35.0	34.8	35.0	33.0	37.6
85-89	34.80699898398832	35.0	33.8	35.0	32.4	38.2
90-94	39.284636519162596	40.6	38.8	40.6	35.8	40.6
95-99	39.51716147259721	41.0	39.0	41.0	36.0	41.0
100-104	39.37054229687935	40.6	39.0	41.0	35.6	41.0
105-109	39.18486055696734	40.0	38.4	41.0	35.0	41.0
110-114	38.947229552997285	40.0	38.0	41.0	35.0	41.0
115-119	38.61894953513022	40.0	37.6	41.0	34.4	41.0
120-124	38.21879875093498	39.8	36.4	41.0	34.0	41.0
125-129	37.674616938223444	38.6	35.2	40.6	33.6	40.8
130-134	37.34716021434431	38.0	35.0	40.0	33.0	41.0
135-139	36.9742929248289	37.0	35.0	40.0	33.0	41.0
140-144	36.57416915578399	35.8	35.0	39.4	33.0	41.0
145-149	36.03561375967065	35.0	35.0	38.2	33.0	40.8
150-154	35.54816887946963	35.0	35.0	36.8	33.0	39.4
155-159	35.12621422659163	35.0	35.0	36.0	33.0	38.2
160-164	34.78796890268168	35.0	34.4	35.0	33.0	36.8
165-169	34.54057060182944	35.0	34.0	35.0	33.0	36.0
170-174	34.38100170267066	35.0	34.0	35.0	33.0	36.0
175-178	34.05944140647759	34.75	33.75	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	61.0
30	554.0
31	3634.0
32	18513.0
33	70865.0
34	215695.0
35	573580.0
36	1401581.0
37	1985099.0
38	1465619.0
39	564071.0
40	15615.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.83725190375506	26.708042961332012	28.896363640970357	22.558341493942567
2	23.456726390080078	27.625383063009195	27.61390225764891	21.30398828926182
3	20.2117915630491	26.21248389520131	30.354220059009755	23.221504482739835
4	21.603835254085265	24.90690254522329	30.557074013030793	22.93218818766065
5	23.039315978367313	25.893903423148597	27.802044945215183	23.264735653268907
6	22.862685767348527	26.536448469078156	27.675455209973638	22.925410553599683
7	22.891744081605246	26.54834099987205	27.174971274233208	23.384943644289493
8	23.29205205222959	26.66406751790372	26.6230533304787	23.420827099387985
9	23.291545313234376	26.907238893231362	26.79536042444458	23.00585536908968
10-14	23.298845521884157	26.706940804017425	26.619667680566938	23.374545993531477
15-19	23.298316613057903	26.636250714185273	26.71608427576229	23.349348396994532
20-24	23.33163786911185	26.42493105182546	26.745392792397904	23.498038286664784
25-29	23.44483069216746	26.35618874000616	26.49400591111038	23.704974656716
30-34	23.522630963526193	26.44296145869887	26.450264834467376	23.584142743307563
35-39	23.536692970643344	26.40169706889497	26.456361538003527	23.605248422458168
40-44	23.579034180812076	26.352356526354864	26.385402243080165	23.6832070497529
45-49	23.6878753827463	26.307506958159827	26.3652657022579	23.63935195683597
50-54	23.687634681723573	26.382330137921684	26.30801686427376	23.622018316080982
55-59	23.640302092451996	26.317926778748884	26.30536915302378	23.736401975775344
60-64	23.581871919185264	26.408981441951145	26.37795317985054	23.63119345901305
65-69	23.595816742909772	26.39061532049341	26.368727363018944	23.64484057357787
70-74	23.55501791955772	26.37324050719506	26.33018669531431	23.74155487793291
75-79	23.48904683661848	26.41651918450493	26.41510664955578	23.679327329320802
80-84	23.516103531844113	26.428981796668445	26.35098199683035	23.703932674657096
85-89	23.48339669682186	26.381041120602617	26.313464308472295	23.82209787410323
90-94	22.034025457949472	26.483565446055223	28.687958334394644	22.79445076160066
95-99	22.868873112030087	26.843367259633112	26.978025585113567	23.309734043223234
100-104	23.12576588752516	26.837688534941588	26.63949373277315	23.397051844760103
105-109	23.080641362803462	26.741422227956246	26.703612158790406	23.474324250449886
110-114	23.107344774300813	26.569545562078943	26.754015858979223	23.56909380464102
115-119	23.227875649553766	26.63842536478115	26.48876393692155	23.644935048743537
120-124	23.228771612390943	26.58636657999473	26.44106036987982	23.74380143773451
125-129	23.20884405114982	26.61428227372159	26.527364214191135	23.649509460937455
130-134	23.41299176030736	26.581532233783122	26.356961380373168	23.64851462553635
135-139	23.499750535893746	26.514141144034454	26.29673030440755	23.689378015664253
140-144	23.447566078326282	26.630787053802813	26.41494408369727	23.506702784173637
145-149	23.406249312155875	26.70970168611334	26.4083933464128	23.475655655317986
150-154	23.289805615957548	26.78836925181587	26.464904993092848	23.456920139133732
155-159	23.18750447372871	26.870684921581173	26.6244732371474	23.317337367542716
160-164	23.12705506753096	27.02167678913025	26.635426901426744	23.215841241912045
165-169	22.982016542197783	27.070002879026607	26.73285152759807	23.215129051177545
170-174	22.831510898500746	27.273727734224135	26.88744080513894	23.007320562136176
175-178	22.762580124639722	27.350915946683074	26.896998987235694	22.98950494144151
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.0
6	1.5
7	10.5
8	27.5
9	50.0
10	103.0
11	213.5
12	395.0
13	673.0
14	1169.5
15	1985.5
16	3108.0
17	4686.0
18	6931.0
19	10112.0
20	14105.5
21	18938.5
22	24423.0
23	30382.5
24	37171.5
25	44510.5
26	52069.5
27	59964.0
28	68109.0
29	76362.5
30	84915.5
31	92957.5
32	101293.0
33	110056.0
34	118201.5
35	126461.5
36	135206.5
37	144382.5
38	152520.5
39	160470.5
40	168852.0
41	176338.5
42	183064.5
43	187746.0
44	191360.0
45	194037.0
46	194994.0
47	194689.0
48	192242.0
49	188200.0
50	184063.0
51	180096.5
52	176188.5
53	172456.0
54	168860.0
55	165578.5
56	162713.5
57	160187.0
58	157227.5
59	152937.0
60	147284.0
61	140760.5
62	131584.0
63	119999.5
64	107845.5
65	94331.0
66	79927.5
67	65955.0
68	52913.0
69	41174.5
70	31150.5
71	22704.0
72	15940.5
73	11005.5
74	7359.0
75	4783.0
76	3035.5
77	1880.0
78	1110.0
79	611.0
80	343.0
81	188.0
82	100.0
83	47.0
84	19.5
85	10.5
86	5.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1538.0
92-93	3040.0
94-95	4277.0
96-97	5358.0
98-99	6602.0
100-101	9046.0
102-103	8889.0
104-105	10543.0
106-107	11486.0
108-109	12934.0
110-111	13709.0
112-113	14522.0
114-115	16000.0
116-117	16129.0
118-119	16963.0
120-121	17649.0
122-123	19093.0
124-125	20016.0
126-127	19943.0
128-129	21770.0
130-131	22129.0
132-133	24206.0
134-135	108797.0
136-137	87530.0
138-139	80087.0
140-141	79220.0
142-143	89008.0
144-145	89220.0
146-147	85070.0
148-149	90791.0
150-151	86090.0
152-153	81017.0
154-155	86271.0
156-157	85704.0
158-159	90612.0
160-161	84959.0
162-163	83243.0
164-165	85703.0
166-167	89443.0
168-169	94936.0
170-171	94622.0
172-173	98884.0
174-175	104635.0
176-177	227220.0
178-179	3915984.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.326554077057
#Duplication Level	Percentage of total
1	92.9007951532682
2	6.437041223630885
3	0.5462866041346832
4	0.0726674958918366
5	0.02063222858806592
6	0.009358013839360215
7	0.004191958015843681
8	0.001056241980755289
9	0.0031936101888941923
>10	0.004777470461466955
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.501356160235938E-5	0.0	0.0	0.0	0.0	0.0
2	2.0586271680511197E-4	0.0	0.0	0.0	0.0	0.0
3	2.0586271680511197E-4	1.583559360039323E-5	1.583559360039323E-5	0.0	0.0	0.0
4	2.0586271680511197E-4	3.167118720078646E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
5	2.0586271680511197E-4	3.167118720078646E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
6	2.0586271680511197E-4	6.334237440157291E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
7	2.2169831040550522E-4	6.334237440157291E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
8	2.2169831040550522E-4	6.334237440157291E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
9	2.2169831040550522E-4	6.334237440157291E-5	3.167118720078646E-5	0.0	3.167118720078646E-5	0.0
10-14	2.3753390400589846E-4	8.234508672204479E-5	3.4838305920865105E-5	0.0	4.433966208110104E-5	0.0
15-19	2.660379724866062E-4	9.501356160235938E-5	6.967661184173021E-5	1.2668474880314582E-5	6.650949312165158E-5	0.0
20-24	2.755393286468422E-4	1.2035051136298856E-4	9.501356160235938E-5	4.750678080117968E-5	1.0134779904251669E-4	0.0
25-29	2.8504068480707815E-4	1.868600044846401E-4	1.1084915520275262E-4	1.1718339264290989E-4	1.5518881728385364E-4	0.0
30-34	3.452159404885724E-4	2.153640729653479E-4	1.1401627392283126E-4	1.4252034240353905E-4	2.121969542452693E-4	3.1671187200786456E-6
35-39	4.275610272106172E-4	2.5336949760629165E-4	1.4885457984369635E-4	2.153640729653479E-4	2.850406848070781E-4	1.583559360039323E-5
40-44	4.433966208110105E-4	2.977091596873927E-4	1.868600044846401E-4	2.7237220992676353E-4	3.6421865280904426E-4	3.167118720078646E-5
45-49	4.8140204545195413E-4	3.0087627840747134E-4	2.1219695424526925E-4	3.0721051584762865E-4	4.940705203322688E-4	4.750678080117969E-5
50-54	5.859169632145495E-4	3.0721051584762865E-4	2.470352601661344E-4	3.230461094480219E-4	5.479115385736056E-4	7.917796800196615E-5
55-59	6.52426456336201E-4	3.167118720078646E-4	2.692050912066849E-4	3.325474656082578E-4	5.954183193747854E-4	9.501356160235938E-5
60-64	7.094345932976167E-4	3.167118720078646E-4	2.8504068480707815E-4	3.768871276893589E-4	6.460922188960437E-4	1.0768203648267395E-4
65-69	7.537742553787178E-4	3.167118720078646E-4	2.8504068480707815E-4	4.497308582511677E-4	7.157688307377738E-4	1.6152305472401095E-4
70-74	9.596369721838298E-4	3.420488217684938E-4	3.0404339712755E-4	4.750678080117969E-4	7.60108492818875E-4	1.7419152960432554E-4
75-79	0.004310448578027037	3.800542464094375E-4	3.578844153688869E-4	5.130732326527407E-4	8.361193421007626E-4	1.9636136064487604E-4
80-84	0.00584650115726518	4.148925523303026E-4	3.6421865280904426E-4	5.384101824133698E-4	0.001010310871705088	2.2169831040550525E-4
85-89	0.005862336750865574	4.433966208110105E-4	3.6421865280904426E-4	5.384101824133698E-4	0.001422036305315312	2.2169831040550525E-4
90-94	0.005881339463186044	4.5606509569132505E-4	3.7688712768935883E-4	5.700813696141563E-4	0.0014727102048365704	2.2169831040550525E-4
95-99	0.005906676412946675	5.162403513728194E-4	3.9272272128975207E-4	6.049196755350215E-4	0.0015043813920373567	2.2169831040550525E-4
100-104	0.005913010650386831	5.479115385736056E-4	4.1172543361022395E-4	6.270895065755719E-4	0.0015328854605180645	2.2169831040550525E-4
105-109	0.005925679125267147	5.669142508940776E-4	4.370623833708532E-4	6.334237440157292E-4	0.0015487210541184578	2.2169831040550525E-4
110-114	0.005944681837587618	5.700813696141563E-4	4.7190068929171824E-4	6.397579814558864E-4	0.0015867264787594016	2.2169831040550525E-4
115-119	0.005976353024788404	5.890840819346282E-4	4.750678080117969E-4	6.587606937763583E-4	0.0016215647846802665	2.2169831040550525E-4
120-124	0.00603336116174982	6.365908627358077E-4	4.909034016121901E-4	6.777634060968302E-4	0.001688074277801918	2.2803254784566253E-4
125-129	0.00612837472335218	6.61927812496437E-4	5.035718764925047E-4	6.809305248169088E-4	0.0017482495334834127	2.50202378886213E-4
130-134	0.00617271438543328	6.650949312165157E-4	5.162403513728194E-4	7.347715430582458E-4	0.0017799207206841989	2.5336949760629165E-4
135-139	0.006274062184475798	6.904318809771448E-4	5.225745888129766E-4	7.696098489791108E-4	0.001887602757166873	2.5336949760629165E-4
140-144	0.006375409983518314	6.999332371373808E-4	5.415773011334484E-4	7.949467987397402E-4	0.0019224410630877382	2.5336949760629165E-4
145-149	0.006460922188960437	7.126017120176953E-4	5.54245776013763E-4	8.424535795409198E-4	0.002049125811890884	2.5336949760629165E-4
150-154	0.0065559357505627965	7.126017120176953E-4	5.54245776013763E-4	8.709576480216277E-4	0.0021599749670936363	2.5336949760629165E-4
155-159	0.006641447956004921	7.126017120176953E-4	5.605800134539202E-4	8.836261229019423E-4	0.0022264844602152877	2.5336949760629165E-4
160-164	0.006682620499365942	7.284373056180885E-4	5.73248488334235E-4	8.994617165023354E-4	0.002280325478456625	2.5336949760629165E-4
165-167	0.006772355529768171	7.60108492818875E-4	5.859169632145495E-4	9.026288352224141E-4	0.0023278322592578046	2.5336949760629165E-4
>>END_MODULE
