##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479061
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6303572
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.22233187786227	39.0	39.0	39.0	37.0	39.0
2	39.98506925914386	41.0	40.0	41.0	38.0	41.0
3	40.034159520982705	41.0	40.0	41.0	38.0	41.0
4	40.05438900356813	41.0	40.0	41.0	38.0	41.0
5	40.060225059696315	41.0	40.0	41.0	38.0	41.0
6	40.04724765577358	41.0	40.0	41.0	38.0	41.0
7	40.06601637928463	41.0	40.0	41.0	38.0	41.0
8	40.038848449736115	41.0	40.0	41.0	38.0	41.0
9	40.02026025878661	41.0	40.0	41.0	38.0	41.0
10-14	39.94366904986569	41.0	40.0	41.0	38.0	41.0
15-19	39.79469605487175	41.0	40.0	41.0	37.8	41.0
20-24	39.57009606616693	40.2	39.0	41.0	36.8	41.0
25-29	39.26114701315381	40.0	38.4	41.0	35.6	41.0
30-34	39.07414088393057	40.0	38.0	41.0	35.0	41.0
35-39	38.78206911890591	40.0	37.2	41.0	35.0	41.0
40-44	38.14715497816159	39.2	35.4	41.0	35.0	41.0
45-49	37.46210738927072	37.8	35.0	40.4	34.0	41.0
50-54	36.83985521225109	36.4	35.0	39.6	34.0	41.0
55-59	36.28623637518537	35.2	35.0	38.8	33.0	41.0
60-64	35.719192863982514	35.0	34.8	37.0	32.8	39.8
65-69	35.46443476809656	35.0	34.8	36.4	33.0	39.0
70-74	35.28320396752825	35.0	35.0	36.0	33.0	38.8
75-79	35.100602705894374	35.0	35.0	35.4	33.0	38.0
80-84	35.00220862076296	35.0	34.8	35.0	33.0	38.0
85-89	34.81112023468599	35.0	33.8	35.0	32.4	38.2
90-94	39.29838377062457	40.6	38.8	40.6	36.2	40.6
95-99	39.53396227682018	41.0	39.0	41.0	36.0	41.0
100-104	39.380950787023586	40.6	39.0	41.0	35.6	41.0
105-109	39.19852906288914	40.0	38.4	41.0	35.0	41.0
110-114	38.95705190560272	40.0	38.0	41.0	35.0	41.0
115-119	38.624317347964606	40.0	37.6	41.0	34.4	41.0
120-124	38.22601009965858	39.8	36.4	41.0	34.0	41.0
125-129	37.68268191755533	38.6	35.2	40.6	33.6	40.8
130-134	37.35587537643128	38.0	35.0	40.0	33.0	41.0
135-139	36.98080155358603	37.0	35.0	40.0	33.0	41.0
140-144	36.57205283330238	35.8	35.0	39.4	33.0	41.0
145-149	36.04041101527919	35.0	35.0	38.2	33.0	40.8
150-154	35.54606294910916	35.0	35.0	36.8	33.0	39.4
155-159	35.126629256195464	35.0	35.0	36.0	33.0	38.2
160-164	34.787928233594975	35.0	34.4	35.0	33.0	36.8
165-169	34.54231247944894	35.0	34.0	35.0	33.0	36.0
170-174	34.37708023046159	35.0	34.0	35.0	33.0	36.0
175-178	34.0497960841944	34.75	33.75	35.0	32.0	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	41.0
30	452.0
31	3337.0
32	16883.0
33	66382.0
34	208376.0
35	567915.0
36	1403208.0
37	1986710.0
38	1469340.0
39	565542.0
40	15382.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.825799721173965	26.684124493223845	28.956613805632742	22.533461979969452
2	23.446483993519866	27.622513076712696	27.639566899529346	21.291436030238092
3	20.184761909596656	26.198368163320733	30.363244839592536	23.253625087490075
4	21.62600823786894	24.86612352488399	30.62414453265545	22.883723704591617
5	23.01388799874103	25.88702088276298	27.830871131479107	23.268219987016884
6	22.857944670101332	26.525151136530205	27.690696639936846	22.92620755343161
7	22.928285740212058	26.526642354525336	27.143705188106043	23.401366717156556
8	23.29266644372429	26.630218549102004	26.61744801201604	23.459666995157665
9	23.29074689715609	26.922497276147556	26.78026046184608	23.00649536485028
10-14	23.308400379975037	26.701003811807023	26.61001095886586	23.380584849352083
15-19	23.301410692223392	26.645654241753725	26.72773468757079	23.32520037845209
20-24	23.353187684696866	26.44057052096811	26.734410267702184	23.471831526632837
25-29	23.4298172528211	26.363433304164687	26.48216915742376	23.724580285590456
30-34	23.541582455153996	26.42344372365383	26.456478961452333	23.578494859739845
35-39	23.52870721552796	26.411184008051308	26.457297544947533	23.602811231473204
40-44	23.55905191532674	26.344209283244485	26.383104690483428	23.71363411094535
45-49	23.67991037462569	26.31701835086519	26.37287556959768	23.63019570491144
50-54	23.68914640778276	26.3675484312704	26.328630814401738	23.614674346545105
55-59	23.631537801107054	26.308150363000532	26.321774384428387	23.738537451464026
60-64	23.579979732126482	26.407954093329938	26.38143579545058	23.630630379092995
65-69	23.59218233725259	26.39680168640891	26.34591625192827	23.665099724410226
70-74	23.523630728736027	26.388520667329573	26.344761351183106	23.743087252751298
75-79	23.50690053195236	26.411009503817834	26.40553324369104	23.67655672053877
80-84	23.52377667773129	26.442077602984465	26.345408603249076	23.688737116035163
85-89	23.469610563661366	26.384119987841814	26.318785602829635	23.827483845667185
90-94	22.028330677586204	26.50902484316874	28.673271504696558	22.789372974548495
95-99	22.859889132951842	26.83901266003997	26.987875593242705	23.31322261376548
100-104	23.1407732334675	26.843185643116446	26.63598182241888	23.380059300997168
105-109	23.09659250381691	26.728341275885892	26.687514253613738	23.48755196668346
110-114	23.1282603816457	26.57434141360222	26.7426993793756	23.55469882537648
115-119	23.224164257192662	26.65112240386862	26.48281845996907	23.641894878969644
120-124	23.240217998681693	26.596346207162096	26.429824505378214	23.733611288777997
125-129	23.216286441183062	26.613258593555667	26.524388531910127	23.646066433351148
130-134	23.405498029401286	26.58869868366472	26.345096517510814	23.660706769423182
135-139	23.498291778066637	26.52544460717987	26.310852273970937	23.66541134078255
140-144	23.438233051335406	26.65470721594473	26.421288360361217	23.485771372358652
145-149	23.40371114597055	26.717208607137195	26.420163009573983	23.458917237318275
150-154	23.27685759380692	26.78690900180804	26.48698337160299	23.44925003278205
155-159	23.190666945818972	26.879812358556897	26.63844673696169	23.29107395866244
160-164	23.118551385530107	27.03741374661887	26.629487621991267	23.214547245859755
165-169	22.960330590710416	27.07785006345539	26.769767314818587	23.19205203101561
170-174	22.833408854793497	27.28767607018047	26.918510620248444	22.96040445477759
175-178	22.755877262036897	27.395998865619976	26.897122244116627	22.951001628226507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.5
5	2.0
6	2.5
7	10.0
8	22.0
9	48.0
10	102.0
11	202.5
12	380.5
13	685.0
14	1155.0
15	1913.0
16	3021.5
17	4598.5
18	6816.0
19	9814.0
20	13671.5
21	18468.5
22	24107.5
23	30215.0
24	36886.5
25	44126.5
26	51780.5
27	59829.0
28	68211.0
29	76450.5
30	84681.5
31	92809.5
32	100874.5
33	109188.5
34	117837.0
35	126546.5
36	134867.5
37	143667.5
38	152391.0
39	161051.0
40	169163.0
41	176056.5
42	182604.5
43	187674.0
44	191557.0
45	194382.0
46	195228.5
47	194876.0
48	192511.0
49	188805.0
50	185143.5
51	180725.5
52	176327.5
53	172217.5
54	167903.0
55	164949.0
56	163107.5
57	160466.0
58	156785.0
59	152394.5
60	146991.0
61	139440.0
62	130505.5
63	120101.5
64	107340.5
65	93340.5
66	79185.0
67	65401.0
68	52613.5
69	41116.5
70	30771.5
71	22331.5
72	15849.0
73	10911.0
74	7338.5
75	4716.0
76	2932.5
77	1827.0
78	1088.0
79	604.5
80	326.5
81	189.5
82	99.0
83	44.0
84	20.0
85	10.0
86	5.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1413.0
92-93	2821.0
94-95	4116.0
96-97	5362.0
98-99	6825.0
100-101	8364.0
102-103	8853.0
104-105	10823.0
106-107	11460.0
108-109	12042.0
110-111	13192.0
112-113	14214.0
114-115	16585.0
116-117	15511.0
118-119	16895.0
120-121	17343.0
122-123	18003.0
124-125	19058.0
126-127	19920.0
128-129	22968.0
130-131	23063.0
132-133	24582.0
134-135	102190.0
136-137	85866.0
138-139	90701.0
140-141	80158.0
142-143	83085.0
144-145	82811.0
146-147	86402.0
148-149	89478.0
150-151	87649.0
152-153	81366.0
154-155	82889.0
156-157	92790.0
158-159	84992.0
160-161	82398.0
162-163	83445.0
164-165	89336.0
166-167	88580.0
168-169	88596.0
170-171	97226.0
172-173	99419.0
174-175	100114.0
176-177	231506.0
178-179	3919162.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.45159910926263
#Duplication Level	Percentage of total
1	93.15069651429668
2	6.191350419248113
3	0.5381755751031262
4	0.079834151888939
5	0.015474694601619715
6	0.009358381984768438
7	0.005240182485620809
8	0.001056295122441201
9	0.0
>10	0.008813785268682858
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.932010612395639E-5	0.0	1.586402122479128E-5	1.586402122479128E-5	0.0	0.0
2	2.5382433959666046E-4	0.0	1.586402122479128E-5	3.172804244958256E-5	1.586402122479128E-5	0.0
3	2.6968836082145173E-4	0.0	1.586402122479128E-5	3.172804244958256E-5	3.172804244958256E-5	0.0
4	2.6968836082145173E-4	0.0	3.172804244958256E-5	4.759206367437383E-5	3.172804244958256E-5	0.0
5	2.6968836082145173E-4	1.586402122479128E-5	4.759206367437383E-5	6.345608489916511E-5	3.172804244958256E-5	0.0
6	2.6968836082145173E-4	1.586402122479128E-5	4.759206367437383E-5	7.932010612395639E-5	3.172804244958256E-5	0.0
7	2.6968836082145173E-4	1.586402122479128E-5	6.345608489916511E-5	9.518412734874767E-5	6.345608489916511E-5	0.0
8	2.6968836082145173E-4	1.586402122479128E-5	6.345608489916511E-5	9.518412734874767E-5	7.932010612395639E-5	0.0
9	2.85552382046243E-4	3.172804244958256E-5	6.345608489916511E-5	1.1104814857353894E-4	7.932010612395639E-5	0.0
10-14	2.918979905361595E-4	5.393767216429035E-5	6.345608489916511E-5	1.1739375706345544E-4	1.1739375706345547E-4	0.0
15-19	3.553540754353246E-4	1.0470254008362243E-4	7.297449763403987E-5	1.4912179951303802E-4	1.427761910231215E-4	0.0
20-24	3.712180966601159E-4	1.2691216979833023E-4	7.932010612395639E-5	1.9671386318741185E-4	1.4594899526807975E-4	0.0
25-29	3.839093136399489E-4	1.649858207378293E-4	7.932010612395639E-5	2.3478751412691089E-4	1.7133142922774578E-4	0.0
30-34	3.997733348647402E-4	1.776770377176623E-4	8.883851885883115E-5	2.506515353517022E-4	2.3161470988195264E-4	0.0
35-39	4.4101979004919757E-4	2.157506886571614E-4	1.078753443285807E-4	2.6968836082145173E-4	3.0458920751599253E-4	1.2691216979833023E-5
40-44	4.981302664584461E-4	2.6334275233153525E-4	1.2373936555337196E-4	3.077620117609508E-4	4.029461391096984E-4	4.1246455184457324E-5
45-49	5.362039173979451E-4	2.85552382046243E-4	1.3325777828824674E-4	3.3314444572061684E-4	5.266855046630704E-4	4.759206367437383E-5
50-54	6.155240235219015E-4	3.5218127119036635E-4	1.427761910231215E-4	3.839093136399489E-4	5.933143938071937E-4	8.566571461387289E-5
55-59	7.07535346625691E-4	4.029461391096984E-4	1.427761910231215E-4	4.188101603344897E-4	6.599432829513172E-4	1.1104814857353894E-4
60-64	7.963738654845221E-4	4.124645518445732E-4	1.5864021224791278E-4	4.664022240088635E-4	7.773370400147725E-4	1.1104814857353894E-4
65-69	8.154106909542717E-4	4.5371100702903055E-4	1.6181301649287105E-4	4.917846579685296E-4	8.947307970782281E-4	1.2691216979833023E-4
70-74	0.001031161379611433	4.886118537235713E-4	1.7450423347270406E-4	5.33031113152987E-4	9.613596862223515E-4	1.5229460375799626E-4
75-79	0.004226175254284396	5.393767216429035E-4	1.7450423347270406E-4	5.552407428676948E-4	0.0010248157711215166	1.5864021224791278E-4
80-84	0.005590481079616446	5.520679386227364E-4	1.935410589424536E-4	6.028328065420685E-4	0.0011517279409198468	1.5864021224791278E-4
85-89	0.005609517905086196	5.901415895622355E-4	2.125778844122031E-4	6.440792617265259E-4	0.0015641924927644203	1.5864021224791278E-4
90-94	0.0056761467942303195	6.155240235219015E-4	2.284419056369944E-4	6.789801084210666E-4	0.0015927477309690442	1.5864021224791278E-4
95-99	0.0057364300748845255	6.282152405017345E-4	2.3796031837186913E-4	7.58300214545023E-4	0.0016086117521938355	1.5864021224791278E-4
100-104	0.0057522940961093165	6.345608489916511E-4	2.3796031837186913E-4	7.709914315248561E-4	0.0016435125988883766	1.5864021224791278E-4
105-109	0.0058094045725185645	6.345608489916511E-4	2.60169948086577E-4	8.185834951992298E-4	0.001665722228603084	1.5864021224791278E-4
110-114	0.005847478223458065	6.472520659714842E-4	2.85552382046243E-4	8.534843418937707E-4	0.001687931858317792	1.5864021224791278E-4
115-119	0.00587286065741773	6.662888914412337E-4	2.85552382046243E-4	8.661755588736036E-4	0.0017577335517068734	1.5864021224791278E-4
120-124	0.005958526372031604	6.821529126660249E-4	2.85552382046243E-4	8.820395800983949E-4	0.0018275352450959552	1.5864021224791278E-4
125-129	0.006079092933340017	7.4560899756519E-4	3.0458920751599253E-4	9.264588395278106E-4	0.001913200959709828	1.6181301649287105E-4
130-134	0.0061774498649337225	7.678186272798979E-4	3.2997164147565854E-4	9.645324904673097E-4	0.0019544474148942854	1.7450423347270406E-4
135-139	0.006253597166812721	7.773370400147725E-4	3.363172499655751E-4	0.0010121245541416834	0.0020242491082833667	1.8402264620757882E-4
140-144	0.006291670817752219	7.773370400147725E-4	3.6487248817019933E-4	0.0010216429668765583	0.002084532388937574	1.935410589424536E-4
145-149	0.006424928596040468	8.217562994441883E-4	3.8073650939499066E-4	0.0010628894220610157	0.0021416428653468224	2.062322759222866E-4
150-154	0.0065677047870635885	8.407931249139376E-4	3.870821178849072E-4	0.0011104814857353894	0.0021860621247762383	2.2209629714707788E-4
155-159	0.006618469654982921	8.630027546286454E-4	3.9660053061978194E-4	0.0011263455069601806	0.0022558638181653197	2.3796031837186913E-4
160-164	0.00665654330592242	8.756939716084785E-4	3.9660053061978194E-4	0.0011390367239400138	0.0023288383157993595	2.3796031837186913E-4
165-167	0.006742209020536293	9.042492098131027E-4	4.071765447696428E-4	0.001152785542334833	0.002384891190793622	2.4853633252173E-4
>>END_MODULE
