##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479062
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6351148
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23171826573716	39.0	39.0	39.0	37.0	39.0
2	39.987432350812796	41.0	40.0	41.0	38.0	41.0
3	40.02811916837712	41.0	40.0	41.0	38.0	41.0
4	40.04695529060258	41.0	40.0	41.0	38.0	41.0
5	40.06855894398934	41.0	40.0	41.0	38.0	41.0
6	40.0434622213181	41.0	40.0	41.0	38.0	41.0
7	40.056508839031935	41.0	40.0	41.0	38.0	41.0
8	40.044726874574486	41.0	40.0	41.0	38.0	41.0
9	40.023184627409094	41.0	40.0	41.0	38.0	41.0
10-14	39.94646048241987	41.0	40.0	41.0	38.0	41.0
15-19	39.79487953988791	41.0	40.0	41.0	37.8	41.0
20-24	39.57257270654061	40.2	39.0	41.0	36.8	41.0
25-29	39.26887693374489	40.0	38.4	41.0	35.6	41.0
30-34	39.08317922995968	40.0	38.0	41.0	35.0	41.0
35-39	38.79106520584939	40.0	37.2	41.0	35.0	41.0
40-44	38.16028872260574	39.2	35.4	41.0	35.0	41.0
45-49	37.476249742566225	37.8	35.0	40.4	34.0	41.0
50-54	36.84515089240559	36.4	35.0	39.6	34.0	41.0
55-59	36.28331240273412	35.2	35.0	38.8	33.2	41.0
60-64	35.704722610778404	35.0	34.8	37.0	32.8	39.8
65-69	35.44210737964223	35.0	34.8	36.0	33.0	39.0
70-74	35.256703197595144	35.0	35.0	36.0	33.0	38.8
75-79	35.08363870594733	35.0	35.0	35.2	33.0	37.6
80-84	34.98950385032753	35.0	35.0	35.0	33.0	37.2
85-89	34.79451299198192	35.0	33.8	35.0	32.4	38.2
90-94	39.30099718884178	40.6	38.8	40.6	36.2	40.6
95-99	39.53760623359046	41.0	39.0	41.0	36.0	41.0
100-104	39.38939704094819	40.6	39.0	41.0	35.8	41.0
105-109	39.20804418064411	40.0	38.4	41.0	35.0	41.0
110-114	38.97003190343224	40.0	38.0	41.0	35.0	41.0
115-119	38.63565170466909	40.0	37.6	41.0	34.4	41.0
120-124	38.23650883979934	39.8	36.4	41.0	34.0	41.0
125-129	37.682478527820074	38.6	35.2	40.6	33.6	40.8
130-134	37.362461685631104	38.0	35.0	40.0	33.0	41.0
135-139	36.993176856922034	37.0	35.0	40.0	33.0	41.0
140-144	36.58308326732363	35.8	35.0	39.4	33.0	41.0
145-149	36.04545950287751	35.0	35.0	38.4	33.0	40.8
150-154	35.55169152304798	35.0	35.0	36.8	33.0	39.4
155-159	35.135154302710745	35.0	35.0	36.0	33.0	38.2
160-164	34.793749819959054	35.0	34.6	35.0	33.0	36.8
165-169	34.546578368932344	35.0	34.0	35.0	33.0	36.0
170-174	34.38203810786508	35.0	34.0	35.0	33.0	36.0
175-178	34.04964045572829	34.75	33.75	35.0	32.0	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	40.0
30	441.0
31	3400.0
32	17983.0
33	68464.0
34	211807.0
35	569914.0
36	1408761.0
37	2002860.0
38	1484013.0
39	568367.0
40	15098.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.790155102668052	26.761413842032965	28.9156857941273	22.53274526117168
2	23.437321882595082	27.66268397461372	27.635901414988282	21.26409272780291
3	20.177391551889517	26.21781133111683	30.43334842771732	23.171448689276332
4	21.5967255053732	24.885469524564694	30.60741144750524	22.910393522556866
5	23.022058374328548	25.92403767004012	27.798927060115748	23.254976895515583
6	22.845460379761263	26.513978260308214	27.732356418083786	22.908204941846734
7	22.863850755800367	26.535533418525283	27.198862315915168	23.40175350975918
8	23.274815828571466	26.64376581997459	26.657826270148327	23.423592081305618
9	23.250788676314897	26.94004296546073	26.826016335944303	22.983152022280066
10-14	23.29029964346603	26.721584822145537	26.618485350994813	23.369630183393618
15-19	23.29111839308421	26.657709755779585	26.71666602636248	23.33450582477373
20-24	23.32933825506822	26.449038819438623	26.74775017052035	23.473872754972803
25-29	23.42164282740695	26.382716951329115	26.504162712001044	23.691477509262892
30-34	23.50555836519634	26.444494759057736	26.47710303712022	23.572843838625708
35-39	23.51940782989154	26.407638430091694	26.486235244399907	23.586718495616854
40-44	23.555300553537723	26.338403702763657	26.409483765769593	23.69681197792903
45-49	23.669903456823867	26.320466788051544	26.37820438131815	23.631425373806437
50-54	23.691710538000375	26.386650098533366	26.316146309297157	23.605493054169106
55-59	23.630553090559374	26.300542830996854	26.34099850924589	23.727905569197883
60-64	23.566577255009648	26.406148935594004	26.40174028380381	23.625533525592537
65-69	23.59692452451116	26.409093285182454	26.356236699255003	23.637745491051383
70-74	23.51629973037945	26.392963917704325	26.359439269876876	23.73129708203934
75-79	23.487643493743178	26.40656460847708	26.440419905188794	23.665371992590945
80-84	23.497581854493077	26.457689224058388	26.353594657217876	23.691134264230655
85-89	23.467130666770792	26.379246712562832	26.332278825812278	23.821343794854098
90-94	22.013327041121318	26.506522689163404	28.71990339174041	22.760246877974872
95-99	22.857157277907607	26.855284110696676	26.99459500724283	23.29296360415289
100-104	23.117163265287402	26.853200457036664	26.660165315993194	23.36947096168274
105-109	23.06619982689112	26.747272144273403	26.72874381619035	23.457784212645127
110-114	23.121054305545492	26.594964668666254	26.75603058203689	23.527950443751365
115-119	23.215567658199397	26.658358112691534	26.50046242141925	23.62561180768982
120-124	23.21457233764287	26.612373156471797	26.45866503486743	23.714389471017903
125-129	23.205600455202998	26.6281905550586	26.529812801672897	23.636396188065504
130-134	23.39456501499731	26.585586956687308	26.36093590248978	23.6589121258256
135-139	23.494512893145473	26.54663888974751	26.306170074598956	23.65267814250807
140-144	23.42394512556602	26.65343982621306	26.423506456448067	23.499108591772856
145-149	23.396879264449396	26.710523229719563	26.436723476735864	23.455874029095177
150-154	23.284142382642443	26.79688025166384	26.486558933485565	23.432418432208152
155-159	23.170795315010796	26.877806387648977	26.63167821700935	23.31972008033088
160-164	23.109064534373758	27.036423639741443	26.657741945979097	23.196769879905705
165-169	22.962466464286642	27.090630000451384	26.770015908500326	23.17688762676165
170-174	22.843021807225217	27.279572365760686	26.89220607686989	22.985199750144208
175-178	22.74037719717226	27.399259003190977	26.89380048619231	22.966563313444453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.0
6	2.5
7	6.5
8	15.5
9	43.5
10	102.5
11	215.0
12	389.0
13	688.0
14	1198.0
15	1953.5
16	3061.5
17	4636.0
18	6874.5
19	9995.5
20	13915.5
21	18691.0
22	24364.5
23	30598.5
24	37263.5
25	44596.0
26	52499.5
27	60554.0
28	68788.5
29	77145.5
30	85600.0
31	94107.5
32	102484.0
33	110829.0
34	119242.0
35	127898.0
36	136464.5
37	145228.5
38	153862.5
39	162022.0
40	169853.5
41	176831.5
42	183663.5
43	189171.0
44	193104.0
45	195623.0
46	196408.5
47	196004.0
48	193762.0
49	190342.0
50	186156.5
51	181826.5
52	177390.0
53	173594.0
54	170253.5
55	166640.5
56	163533.5
57	160578.5
58	157630.5
59	153655.5
60	148037.5
61	140615.0
62	131334.5
63	120596.0
64	107890.5
65	93760.0
66	79475.5
67	65498.0
68	52549.5
69	41012.5
70	30969.0
71	22646.0
72	15981.5
73	10909.0
74	7313.0
75	4782.5
76	3005.0
77	1855.5
78	1111.0
79	623.5
80	351.0
81	188.0
82	92.5
83	46.5
84	21.5
85	8.5
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1391.0
92-93	2714.0
94-95	4094.0
96-97	5306.0
98-99	6966.0
100-101	7382.0
102-103	8590.0
104-105	10027.0
106-107	12044.0
108-109	12114.0
110-111	12747.0
112-113	13841.0
114-115	14922.0
116-117	15243.0
118-119	15992.0
120-121	17422.0
122-123	17738.0
124-125	18631.0
126-127	19612.0
128-129	20240.0
130-131	21351.0
132-133	23574.0
134-135	103171.0
136-137	81704.0
138-139	80411.0
140-141	79857.0
142-143	86704.0
144-145	83685.0
146-147	88322.0
148-149	86912.0
150-151	85597.0
152-153	85135.0
154-155	84460.0
156-157	84451.0
158-159	83644.0
160-161	87396.0
162-163	84131.0
164-165	91215.0
166-167	88670.0
168-169	93566.0
170-171	96968.0
172-173	108944.0
174-175	105154.0
176-177	235677.0
178-179	3963433.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.42275867742352
#Duplication Level	Percentage of total
1	93.09356340862762
2	6.244337197707422
3	0.5513059007543623
4	0.07060913047101477
5	0.01753357667851661
6	0.003118484113836271
7	0.003142932730199276
8	0.0031675063983849718
9	0.0010640683570237353
>10	0.009263870539990208
>50	0.0028939236216251095
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.447110978991514E-5	3.149036992997172E-5	3.149036992997172E-5	0.0	0.0	0.0
2	1.7319703461484443E-4	3.149036992997172E-5	3.149036992997172E-5	1.574518496498586E-5	0.0	0.0
3	1.7319703461484443E-4	6.298073985994344E-5	3.149036992997172E-5	3.149036992997172E-5	0.0	0.0
4	1.7319703461484443E-4	6.298073985994344E-5	3.149036992997172E-5	3.149036992997172E-5	0.0	0.0
5	1.7319703461484443E-4	6.298073985994344E-5	4.723555489495757E-5	3.149036992997172E-5	1.574518496498586E-5	0.0
6	1.7319703461484443E-4	6.298073985994344E-5	4.723555489495757E-5	3.149036992997172E-5	1.574518496498586E-5	0.0
7	1.7319703461484443E-4	6.298073985994344E-5	4.723555489495757E-5	4.723555489495757E-5	1.574518496498586E-5	0.0
8	1.7319703461484443E-4	6.298073985994344E-5	4.723555489495757E-5	4.723555489495757E-5	1.574518496498586E-5	0.0
9	1.7319703461484443E-4	6.298073985994344E-5	4.723555489495757E-5	4.723555489495757E-5	1.574518496498586E-5	0.0
10-14	1.7319703461484443E-4	7.872592482492929E-5	5.3533628880951916E-5	6.927881384593778E-5	3.463940692296889E-5	0.0
15-19	1.8264414559383593E-4	1.2281244272688968E-4	7.557688783193211E-5	9.762014678291231E-5	6.927881384593777E-5	0.0
20-24	1.9209125657282744E-4	1.4800473867086707E-4	8.817303580392082E-5	1.1651436874089535E-4	8.502399881092363E-5	0.0
25-29	2.0468740454481616E-4	1.7004799762184727E-4	1.10216294754901E-4	1.5115377566386426E-4	1.0391822076890665E-4	0.0
30-34	2.1728355251680485E-4	1.8894221957983028E-4	1.10216294754901E-4	1.8894221957983028E-4	1.6060088664285576E-4	0.0
35-39	2.424758484607822E-4	2.0468740454481616E-4	1.10216294754901E-4	2.109854785308105E-4	2.424758484607822E-4	0.0
40-44	3.086056253137228E-4	2.2673066349579636E-4	1.10216294754901E-4	2.330287374817907E-4	3.086056253137228E-4	3.149036992997172E-6
45-49	3.747354021666634E-4	2.5822103342576806E-4	1.1966340573389252E-4	2.5507199643277093E-4	3.841825131456549E-4	1.574518496498586E-5
50-54	3.8733155013865206E-4	2.8026429237674826E-4	1.6060088664285576E-4	2.8341332936974545E-4	4.40865179019604E-4	5.038459188795475E-5
55-59	4.188219200686238E-4	3.1490369929971715E-4	1.857931825868331E-4	3.054565883207256E-4	5.164420668515362E-4	7.242785083893494E-5
60-64	4.2826903104761533E-4	3.337979212577002E-4	2.2673066349579636E-4	3.5899021720167755E-4	6.455525835644203E-4	7.872592482492929E-5
65-69	4.660574749635814E-4	3.7158636517366624E-4	2.3617777447478786E-4	4.156728830756266E-4	7.683650262913099E-4	7.872592482492929E-5
70-74	7.116823604173607E-4	4.093748090896323E-4	2.5192295943977374E-4	4.5975940097758703E-4	8.691342100672192E-4	9.762014678291231E-5
75-79	0.004172474015721252	4.4401421601260126E-4	2.6766814440475957E-4	4.975478448935531E-4	9.510091718851457E-4	1.1336533174789818E-4
80-84	0.005636776217464937	4.7865362293557004E-4	2.8341332936974545E-4	5.353362888095191E-4	0.0010643745036330442	1.2596147971988687E-4
85-89	0.005709204068303872	5.16442066851536E-4	2.8341332936974545E-4	5.85720880697474E-4	0.0014233647208347214	1.3225955370588122E-4
90-94	0.005775333845156812	5.353362888095191E-4	2.8341332936974545E-4	5.983170286694625E-4	0.001448557016778699	1.4800473867086707E-4
95-99	0.005797377104107793	5.51081473774505E-4	2.92860440348737E-4	6.172112506274456E-4	0.001448557016778699	1.5745184964985857E-4
100-104	0.005857208806974739	5.605285847534965E-4	3.1175466230672E-4	6.549996945434117E-4	0.001470600275729679	1.5745184964985857E-4
105-109	0.005872953991939725	5.825718437044768E-4	3.3064888426470303E-4	7.211294713963522E-4	0.0015083887196456452	1.5745184964985857E-4
110-114	0.0058792520659257195	5.983170286694625E-4	3.3064888426470303E-4	7.526198413263241E-4	0.001539879089575617	1.5745184964985857E-4
115-119	0.005907593398862693	6.203602876204428E-4	3.4639406922968885E-4	7.872592482492929E-4	0.001599710792442563	1.5745184964985857E-4
120-124	0.005961127027743646	6.298073985994343E-4	3.6528829118767186E-4	7.872592482492929E-4	0.0016500953843305178	1.6374992363585292E-4
125-129	0.00602410776760359	6.707448795083977E-4	3.841825131456549E-4	8.030044332142788E-4	0.0016973309392254755	2.2358162650279917E-4
130-134	0.006071343322498547	7.116823604173607E-4	4.030767351036379E-4	8.09302507200273E-4	0.0017571626420924218	2.6137007041876525E-4
135-139	0.00617841058026045	7.368746563613381E-4	4.345671050336097E-4	8.187496181792646E-4	0.0018484847148893396	2.6766814440475957E-4
140-144	0.006260285542078377	7.526198413263241E-4	4.40865179019604E-4	8.187496181792646E-4	0.0018862731588053059	2.8026429237674826E-4
145-149	0.0063169682079523256	8.061534702072758E-4	4.471632530055984E-4	8.218986551722619E-4	0.0019335087137002632	2.8341332936974545E-4
150-154	0.00642403546571423	8.187496181792646E-4	4.5661036398458984E-4	8.565380620952307E-4	0.0019649990836302354	2.8341332936974545E-4
155-159	0.006471271020609187	8.281967291582561E-4	4.6290843797058416E-4	8.691342100672194E-4	0.001983893305588218	2.8341332936974545E-4
160-164	0.006556295019420111	8.344948031442504E-4	4.723555489495757E-4	8.848793950322053E-4	0.002034277897476173	2.8341332936974545E-4
165-167	0.006665461635177347	8.344948031442505E-4	4.723555489495757E-4	8.97475543004194E-4	0.0020573708354248185	2.8341332936974545E-4
>>END_MODULE
