##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479071
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10470796
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.18501735684661	39.0	39.0	39.0	37.0	39.0
2	39.87016125612609	41.0	40.0	41.0	38.0	41.0
3	39.90542552829795	41.0	40.0	41.0	38.0	41.0
4	39.90075148059422	41.0	40.0	41.0	38.0	41.0
5	39.89693973600479	41.0	40.0	41.0	38.0	41.0
6	39.872191283260605	41.0	40.0	41.0	38.0	41.0
7	39.88083121856256	41.0	40.0	41.0	38.0	41.0
8	39.844885909342516	41.0	40.0	41.0	38.0	41.0
9	39.81208945337107	41.0	40.0	41.0	38.0	41.0
10-14	39.698587729146865	40.0	39.6	41.0	37.6	41.0
15-19	39.638923802927685	40.2	39.4	41.0	37.0	41.0
20-24	39.64303550561008	40.2	39.4	41.0	37.0	41.0
25-29	39.44689723684809	40.0	39.0	41.0	36.4	41.0
30-34	39.1361132238657	40.0	38.2	41.0	35.2	41.0
35-39	38.70372239130626	40.0	37.6	41.0	35.0	41.0
40-44	38.215391723800174	39.4	36.2	41.0	34.0	41.0
45-49	37.84460398235244	38.8	35.2	40.8	34.0	41.0
50-54	37.229140611659325	37.4	35.0	40.0	34.0	41.0
55-59	36.549197673223695	36.2	35.0	39.0	33.0	41.0
60-64	35.62455559252611	35.0	34.6	37.6	32.4	39.8
65-69	35.29285769677874	35.0	34.6	36.4	32.8	39.0
70-74	35.18238821575743	35.0	34.6	36.0	33.0	38.6
75-79	35.00356582250289	35.0	34.0	35.6	33.0	38.0
80-84	34.953303397373034	35.0	34.0	35.0	33.0	38.4
85-89	34.665473799699654	34.8	33.4	35.0	31.8	39.0
90-94	39.38661772212819	40.6	38.8	40.6	36.8	40.8
95-99	39.520131207014394	40.4	39.0	41.0	36.4	41.0
100-104	39.31470998031862	40.0	38.8	41.0	36.0	41.0
105-109	39.321726834680604	40.0	39.0	41.0	35.6	41.0
110-114	39.1659362513333	40.0	38.0	41.0	35.0	41.0
115-119	38.91883812720978	40.0	38.0	41.0	35.0	41.0
120-124	38.5951639894944	40.0	37.2	41.0	34.6	41.0
125-129	37.98841662022427	39.4	36.2	40.4	33.8	40.6
130-134	38.06892773781202	39.0	35.4	40.8	34.0	41.0
135-139	37.63475683161914	38.6	35.0	40.8	34.0	41.0
140-144	37.01197008796366	36.8	35.0	39.8	34.0	41.0
145-149	36.34148099936771	35.8	35.0	38.8	33.2	40.8
150-154	35.67648639615424	35.0	35.0	37.0	33.0	39.2
155-159	35.12784016157074	35.0	35.0	36.0	33.0	37.8
160-164	34.71987493329789	35.0	34.2	35.2	33.0	36.6
165-169	34.46870601916957	35.0	34.0	35.0	33.0	36.0
170-174	34.3081236990885	35.0	34.0	35.0	32.6	35.6
175-178	33.74754137136774	34.75	33.25	35.0	30.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	8.0
30	219.0
31	3086.0
32	21630.0
33	96723.0
34	310058.0
35	827893.0
36	1971757.0
37	3375624.0
38	3207940.0
39	652724.0
40	3134.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.35974189545857	27.341340620140052	29.114777902272188	22.184139582129188
2	22.323976133237625	28.149817836198892	29.311362765543326	20.214843265020157
3	21.034141052886525	26.945105224091847	29.659015417739017	22.361738305282618
4	21.287999498796463	26.754661250204855	30.32357807372047	21.633761177278213
5	22.239455338448003	27.188782973137858	28.722114345461414	21.84964734295272
6	22.769166737657766	26.951609027623114	27.968570870829684	22.310653363889433
7	22.387829922386036	27.904783934287323	28.061868457756223	21.64551768557042
8	22.381058708430572	27.909119803308176	28.072899137754188	21.636922350507067
9	22.919842961318317	27.575152834607795	27.314886088889516	22.190118115184365
10-14	22.39989586274052	27.973420549879872	27.706638540183576	21.92004504719603
15-19	22.34853205047639	27.95326162404463	27.688796534666515	22.009409790812466
20-24	22.18831500489552	27.912032666857417	27.81156657048805	22.08808575775901
25-29	22.06438555387766	28.08638808358027	27.75045564826208	22.098770714279983
30-34	22.11656496793558	28.066842291646214	27.689235851791977	22.12735688862623
35-39	22.011959740214593	28.04026742570479	27.723479666684366	22.22429316739625
40-44	22.029669950594013	28.146295658897376	27.706657640928157	22.11737674958045
45-49	22.35923801781641	27.938981907392712	27.4886646631259	22.213115411664976
50-54	22.276803024335496	27.976772730554583	27.562275112608443	22.18414913250148
55-59	22.183501617260042	28.02268328023963	27.62115124771794	22.17266385478239
60-64	22.19885097560873	28.00424151134259	27.593621344547252	22.20328616850142
65-69	22.151540341345584	27.962363128839492	27.610252362857608	22.275844166957317
70-74	21.993806392560796	28.194544139719657	27.548768976112225	22.262880491607323
75-79	22.071787092404435	28.19812743940384	27.394226761747625	22.335858706444096
80-84	22.068377609495972	28.283933714304048	27.29584264653805	22.351846029661928
85-89	22.11973378146227	28.417526232007578	27.136318957985623	22.326421028544534
90-94	21.5970878016226	27.193475931029333	29.281421694817244	21.928014572530824
95-99	22.264305346791094	27.635500461457863	28.024725481531892	22.075468710219152
100-104	22.241722206290266	27.706067142207825	27.857932287231723	22.19427836427019
105-109	22.015531141639165	27.814481505693685	28.07358896402819	22.09639838863896
110-114	22.031599830725053	27.696153753529757	28.026132905859964	22.24611350988523
115-119	21.990235187712624	27.749840445005052	27.97120795362817	22.28871641365415
120-124	21.87200149218674	27.848243779951616	28.054955255598042	22.2247994722636
125-129	21.9244294461635	27.845686607463886	27.993407761632046	22.236476184740567
130-134	21.986040568177337	27.83049820340418	27.98704301908738	22.196418209331096
135-139	21.93971424756407	27.923386808032923	28.0303313317877	22.106567612615304
140-144	21.940519587552746	27.98624085056102	28.03986658697097	22.03337297491526
145-149	21.842120148816125	28.03179089742742	28.116435035824637	22.009653917931818
150-154	21.66445437824104	28.22371661075385	28.283846689031794	21.827982321973312
155-159	21.596576457373022	28.306901165915292	28.240827584616074	21.855694792095605
160-164	21.47003425082941	28.540477221581767	28.225331309641348	21.76415721794748
165-169	21.281899100631556	28.818714190489935	28.30292861367496	21.59645809520355
170-174	21.33824178551881	29.100580138012283	28.140373872530468	21.420804203938435
175-178	21.32380419071598	29.325327588975885	28.096602418061313	21.254265802246827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.5
7	6.5
8	12.0
9	23.0
10	44.5
11	107.0
12	227.5
13	433.5
14	814.0
15	1297.5
16	2036.5
17	3319.5
18	5262.5
19	8158.0
20	12365.0
21	17858.5
22	24863.0
23	34402.5
24	46450.0
25	60759.0
26	77521.5
27	96739.5
28	118124.5
29	141339.5
30	165891.0
31	191602.5
32	217720.5
33	244148.0
34	270346.0
35	295666.0
36	319415.5
37	341112.5
38	360533.0
39	376728.5
40	389661.0
41	398488.0
42	403077.0
43	403801.5
44	400027.5
45	391783.0
46	379247.5
47	363047.0
48	344510.0
49	324589.5
50	303687.0
51	281765.5
52	260199.5
53	239740.0
54	221318.5
55	204972.5
56	190134.5
57	177654.5
58	166348.0
59	155825.0
60	145765.5
61	135027.5
62	123962.5
63	112649.5
64	100759.0
65	87754.5
66	74919.5
67	62611.5
68	50757.0
69	39879.5
70	30421.0
71	22834.0
72	16538.0
73	11525.5
74	7819.0
75	5204.5
76	3357.5
77	2067.0
78	1239.5
79	720.5
80	413.0
81	227.5
82	110.5
83	54.0
84	32.5
85	16.0
86	9.5
87	4.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1435.0
92-93	3231.0
94-95	5190.0
96-97	6850.0
98-99	8599.0
100-101	10469.0
102-103	12023.0
104-105	13667.0
106-107	15626.0
108-109	17896.0
110-111	19271.0
112-113	20778.0
114-115	22331.0
116-117	24131.0
118-119	25745.0
120-121	27379.0
122-123	29339.0
124-125	31357.0
126-127	33055.0
128-129	34931.0
130-131	36952.0
132-133	42229.0
134-135	120198.0
136-137	124939.0
138-139	122579.0
140-141	124708.0
142-143	129696.0
144-145	130608.0
146-147	130327.0
148-149	130330.0
150-151	137984.0
152-153	141372.0
154-155	135231.0
156-157	136581.0
158-159	142273.0
160-161	147602.0
162-163	157610.0
164-165	158998.0
166-167	164237.0
168-169	171405.0
170-171	179869.0
172-173	182610.0
174-175	195109.0
176-177	830290.0
178-179	6133756.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.50508136811216
#Duplication Level	Percentage of total
1	82.68369696628618
2	13.386009193895093
3	2.539185737053295
4	0.694071660484412
5	0.2830285141773855
6	0.13093738961216003
7	0.08325372990438186
8	0.045439441852810104
9	0.03424148108640506
>10	0.11882791982608568
>50	0.0013079658218126378
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4325558438919067E-4	9.550372292612711E-6	9.550372292612711E-6	1.9100744585225423E-5	0.0	0.0
2	9.072853677982076E-4	9.550372292612711E-6	1.9100744585225423E-5	3.8201489170450846E-5	0.0	0.0
3	9.072853677982076E-4	3.8201489170450846E-5	1.9100744585225423E-5	4.7751861463063554E-5	0.0	0.0
4	9.168357400908202E-4	3.8201489170450846E-5	2.865111687783813E-5	5.730223375567626E-5	0.0	0.0
5	9.263861123834329E-4	3.8201489170450846E-5	2.865111687783813E-5	7.640297834090169E-5	0.0	0.0
6	9.263861123834329E-4	3.8201489170450846E-5	2.865111687783813E-5	8.595335063351439E-5	0.0	0.0
7	9.359364846760457E-4	3.8201489170450846E-5	3.8201489170450846E-5	8.595335063351439E-5	0.0	0.0
8	9.359364846760457E-4	3.8201489170450846E-5	4.7751861463063554E-5	8.595335063351439E-5	0.0	0.0
9	9.359364846760457E-4	3.8201489170450846E-5	4.7751861463063554E-5	8.595335063351439E-5	0.0	0.0
10-14	9.588573781783162E-4	4.966193592158609E-5	7.067275496533407E-5	1.0505409521873981E-4	0.0	0.0
15-19	0.0010027890907243348	8.404327617499185E-5	9.741379738464964E-5	1.5089588222328082E-4	3.8201489170450846E-6	0.0
20-24	0.0010314402076021727	1.1460446751135254E-4	1.3370521209657795E-4	1.7381677572555132E-4	2.2920893502270507E-5	0.0
25-29	0.0010658215478555786	1.356152865551005E-4	1.4707573330623576E-4	2.2920893502270502E-4	3.2471265794883215E-5	0.0
30-34	0.00205141996845321	1.489858077647583E-4	1.7572685018407388E-4	2.903313176954264E-4	5.921230821419881E-5	0.0
35-39	0.005726403226650582	1.6044625451589354E-4	2.2920893502270507E-4	3.4381340253405757E-4	8.786342509203694E-5	1.9100744585225423E-6
40-44	0.008780612285828125	1.79546999101119E-4	2.483096796079305E-4	3.9156526399712117E-4	1.5662610559884845E-4	2.8651116877838134E-5
45-49	0.010960007243002346	1.8909737139373165E-4	2.483096796079305E-4	4.794286890891581E-4	2.024678926033895E-4	6.876268050681152E-5
50-54	0.012581660458287985	1.9100744585225421E-4	2.597701263590657E-4	5.310006994692667E-4	2.3875930731531773E-4	7.831305279942424E-5
55-59	0.014123090546315675	1.9100744585225421E-4	2.7123057311020097E-4	5.577417418885823E-4	3.4763355145110274E-4	1.0314402076021726E-4
60-64	0.015614858698421783	2.1774848827156977E-4	3.208925090317871E-4	6.150439756442586E-4	4.163962319579142E-4	1.489858077647583E-4
65-69	0.016927079851426767	2.330290839397501E-4	3.62914147119283E-4	6.742562838584574E-4	5.004395081329061E-4	1.6999662680850625E-4
70-74	0.031779818840898054	2.483096796079305E-4	3.858350406215535E-4	7.04817475194818E-4	5.940331566005105E-4	1.814570735596415E-4
75-79	0.32770574462533697	2.655003497346334E-4	4.08755934123824E-4	7.334685920726562E-4	6.608857626487994E-4	1.8909737139373165E-4
80-84	0.4618712846664188	2.7505072202724603E-4	4.221264553334818E-4	7.716700812431069E-4	8.366126128328735E-4	2.0055781814486696E-4
85-89	0.4626544151944131	2.8651116877838133E-4	4.3358690208461704E-4	8.117816448720806E-4	0.0011708756430743182	2.0055781814486696E-4
90-94	0.46297339762898637	3.151622856562194E-4	4.5077757221131996E-4	8.499831340425312E-4	0.001184246164283976	2.0055781814486696E-4
95-99	0.4634108046799881	3.495436259096252E-4	4.641480934209777E-4	9.072853677982076E-4	0.0011899763876595435	2.0055781814486696E-4
100-104	0.46394371545391583	3.610040726607605E-4	4.8324883800620315E-4	9.359364846760456E-4	0.0011937965365765887	2.0628804152043458E-4
105-109	0.4645167377914726	3.762846683289408E-4	4.908891358402933E-4	9.951487928902445E-4	0.001201436834410679	2.1010819043747962E-4
110-114	0.46511077094807307	4.221264553334818E-4	5.080798059669963E-4	0.0010371704309777403	0.0012167174300788594	2.1010819043747962E-4
115-119	0.46569525373238096	4.7369846571359046E-4	5.252704760936991E-4	0.0010791920690652363	0.0012606491426248778	2.1010819043747962E-4
120-124	0.46810958784795353	4.870689869232482E-4	5.424611462204019E-4	0.0011212137071527322	0.0012644692915419228	2.2538878610565998E-4
125-129	0.4731693750885797	5.138100293425638E-4	5.692021886397177E-4	0.001188066313201021	0.001289300259502716	2.4066938177384032E-4
130-134	0.4788804977195621	5.271805505522216E-4	5.844827843078978E-4	0.001243458472498175	0.0013217715252975993	2.483096796079305E-4
135-139	0.4855313769841375	5.348208483863118E-4	6.245943479368714E-4	0.0012988506317953286	0.0013294118231316893	2.483096796079305E-4
140-144	0.49259101218283696	5.348208483863118E-4	6.417850180635742E-4	0.0013408722698828248	0.0013714334612191853	2.483096796079305E-4
145-149	0.4975094539135324	5.558316674300597E-4	6.58975688190277E-4	0.0013905342058044108	0.0013943543547214558	2.483096796079305E-4
150-154	0.5018854344980076	5.730223375567627E-4	6.685260604828898E-4	0.0014287356949748615	0.0014077248759311136	2.5786005190054316E-4
155-159	0.5073195963325043	5.730223375567627E-4	6.742562838584575E-4	0.0014726674075208799	0.001451656588477132	2.674104241931559E-4
160-164	0.513997216639499	5.978533055175558E-4	6.818965816925476E-4	0.001522329343442466	0.001476487556437925	2.674104241931559E-4
165-167	0.5200718901090869	6.112238267272135E-4	6.876268050681151E-4	0.0015567106836958718	0.0014994084499401958	2.674104241931559E-4
>>END_MODULE
