##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479072
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10462250
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.18741800281966	39.0	38.0	39.0	37.0	39.0
2	39.868657793495665	41.0	40.0	41.0	38.0	41.0
3	39.89878764128175	41.0	40.0	41.0	38.0	41.0
4	39.89455088532581	41.0	40.0	41.0	38.0	41.0
5	39.88480871705417	41.0	40.0	41.0	38.0	41.0
6	39.85658008554565	41.0	40.0	41.0	38.0	41.0
7	39.86841750101556	41.0	40.0	41.0	38.0	41.0
8	39.833456665631196	41.0	40.0	41.0	38.0	41.0
9	39.800981433248104	41.0	40.0	41.0	38.0	41.0
10-14	39.68954328179884	40.2	39.6	41.0	37.4	41.0
15-19	39.61663185261297	40.0	39.0	41.0	37.0	41.0
20-24	39.6039508518722	40.0	39.0	41.0	37.0	41.0
25-29	39.40524644316471	40.0	39.0	41.0	36.2	41.0
30-34	39.09964019211928	40.0	38.0	41.0	35.2	41.0
35-39	38.67135304547301	40.0	37.6	41.0	34.8	41.0
40-44	38.21055979354345	39.2	36.2	41.0	34.0	41.0
45-49	37.867424005352575	39.0	35.2	40.8	34.0	41.0
50-54	37.25876279003082	37.4	35.0	40.0	34.0	41.0
55-59	36.57960746493345	36.2	35.0	39.2	33.0	41.0
60-64	35.66432004587923	35.0	34.6	37.6	32.4	39.8
65-69	35.33776539463308	35.0	34.6	36.8	32.8	39.0
70-74	35.22100439198069	35.0	34.8	36.0	33.0	38.8
75-79	35.03972067193959	35.0	34.0	35.8	33.0	38.0
80-84	34.980551009582065	35.0	34.0	35.0	33.0	38.6
85-89	34.70762468876198	34.8	33.4	35.0	32.2	39.0
90-94	39.33239198196245	40.6	38.8	40.6	36.8	40.8
95-99	39.48731747736384	40.2	39.0	41.0	36.4	41.0
100-104	39.278207516281725	40.0	38.6	41.0	35.8	41.0
105-109	39.26792428199305	40.0	38.8	41.0	35.2	41.0
110-114	39.10240037425416	40.0	38.0	41.0	35.0	41.0
115-119	38.84825163629938	40.0	38.0	41.0	35.0	41.0
120-124	38.51790330191818	40.0	37.2	41.0	34.2	41.0
125-129	37.92753448472271	39.4	35.8	40.4	33.8	40.6
130-134	37.99597305965803	39.0	35.4	40.8	34.0	41.0
135-139	37.554600508058165	38.2	35.0	40.8	34.0	41.0
140-144	36.92932761123401	36.8	35.0	39.6	33.8	41.0
145-149	36.24829621130807	35.6	35.0	38.4	33.0	40.6
150-154	35.584978655127465	35.0	35.0	36.8	33.0	39.2
155-159	35.04522649774263	35.0	34.8	36.0	33.0	37.6
160-164	34.657750122843495	35.0	34.0	35.0	33.0	36.4
165-169	34.41327819318114	35.0	34.0	35.0	32.8	36.0
170-174	34.263136579491274	35.0	34.0	35.0	32.0	35.6
175-178	33.735656538302884	34.75	33.25	35.0	31.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	197.0
31	3139.0
32	22081.0
33	99590.0
34	319759.0
35	854743.0
36	2009824.0
37	3379819.0
38	3145448.0
39	624698.0
40	2946.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.306850820808144	27.427656574828553	29.156854405123184	22.108638199240126
2	22.251150565127002	28.233726014958542	29.341470524982675	20.173652894931777
3	20.998255633348467	26.991708284546824	29.686692633037826	22.323343449066883
4	21.224287318693396	26.80677674496404	30.37306506726565	21.59587086907692
5	22.204697842242346	27.233033047384648	28.784219455662026	21.778049654710983
6	22.719166527276638	27.031231331692513	28.01882004348969	22.23078209754116
7	22.336734450046595	27.919032712848573	28.124084207507945	21.620148629596883
8	22.3042940094148	27.980071208392076	28.145112189060672	21.570522593132452
9	22.891337905326292	27.629467848694116	27.351994073932474	22.12720017204712
10-14	22.34813926258692	28.040098449186363	27.75748046548305	21.854281822743673
15-19	22.29838705823317	28.009380391407202	27.730857129202608	21.96137542115702
20-24	22.129817199933093	27.96806231929078	27.862366125833354	22.03975435494277
25-29	22.020657124423522	28.147132786924416	27.796993954455303	22.035216134196755
30-34	22.06582905206815	28.119370116370764	27.715967406628593	22.098833424932497
35-39	21.956581041362995	28.089976821429424	27.76991756075414	22.18352457645344
40-44	21.98022031589763	28.18613204616598	27.74479103443332	22.08885660350307
45-49	22.32195177901503	27.988776792754905	27.52557432674616	22.163697101483905
50-54	22.228673564481827	28.015675404430212	27.616071112810342	22.139579918277615
55-59	22.13809457812612	28.06981672202442	27.669157208057538	22.12293149179192
60-64	22.16543860068341	28.05644674902626	27.61302014385051	22.16509450643982
65-69	22.107533274391265	28.025789863557076	27.641664077994694	22.225012784056965
70-74	21.94582618461612	28.220784248130183	27.60499127816674	22.228398289086957
75-79	22.02486654400344	28.24855074195321	27.429505125570504	22.297077588472845
80-84	22.0332968529714	28.34979664986021	27.311297283089203	22.30560921407919
85-89	22.086923940834907	28.470493440703482	27.180027240794285	22.26255537766733
90-94	21.542990708474182	27.23839074556312	29.326779998811887	21.89183854715081
95-99	22.20747914700101	27.691358846161624	28.061896975128313	22.039265031709053
100-104	22.190999060938786	27.7496416714856	27.914588964389292	22.14477030318632
105-109	21.94294080723039	27.870762549921412	28.12007647406047	22.066220168787734
110-114	21.969069740166805	27.75303711115469	28.070334975775207	22.2075581729033
115-119	21.933760796420987	27.80691381069881	28.00724165527307	22.25208373760714
120-124	21.819910062890468	27.91833688828007	28.08126944391053	22.180483604918937
125-129	21.86996830804854	27.906317119876338	28.031481004863323	22.192233567211797
130-134	21.93725844217566	27.876615909850187	28.028060472012417	22.158065175961735
135-139	21.87783376222266	28.002973844945533	28.068304791838916	22.05088760099289
140-144	21.87768781981144	28.039448147261204	28.095792692264705	21.98707134066265
145-149	21.77755264586593	28.086629068829904	28.169649683956038	21.966168601348127
150-154	21.589173530894648	28.29291724218249	28.348725849164513	21.769183377758345
155-159	21.5259309358326	28.37229705495788	28.306117319277863	21.795654689931652
160-164	21.396451815254004	28.602619540504847	28.30239344476849	21.69853519947266
165-169	21.20270875826427	28.901010520663235	28.362459627346702	21.533821093725795
170-174	21.263402684750424	29.168470711241202	28.225975557427287	21.342151046581087
175-178	21.23686883731507	29.400778320352643	28.172975284635704	21.189377557696584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.5
7	4.0
8	7.0
9	17.5
10	48.5
11	105.5
12	215.5
13	434.5
14	764.5
15	1289.5
16	2155.0
17	3402.0
18	5404.0
19	8383.0
20	12525.0
21	18178.0
22	25474.0
23	34893.5
24	47005.5
25	61499.0
26	78329.0
27	98207.5
28	119635.0
29	142514.5
30	167430.0
31	193043.0
32	219329.0
33	246357.0
34	272444.0
35	297782.0
36	321635.5
37	342985.0
38	361781.0
39	378148.5
40	391210.0
41	399975.0
42	405031.0
43	405319.0
44	401323.5
45	392143.0
46	378424.5
47	362766.0
48	344356.5
49	323524.0
50	301579.0
51	279719.0
52	258295.0
53	237540.5
54	219246.0
55	202905.5
56	188025.0
57	175573.5
58	164553.0
59	153814.5
60	143546.0
61	133098.5
62	122088.5
63	110522.5
64	98453.5
65	86087.0
66	73475.5
67	61077.0
68	49604.5
69	39168.5
70	29818.0
71	22094.0
72	16071.0
73	11142.5
74	7438.5
75	5042.5
76	3284.0
77	1978.5
78	1163.5
79	683.0
80	386.5
81	212.5
82	108.5
83	55.0
84	35.0
85	18.0
86	4.5
87	3.0
88	2.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1508.0
92-93	3420.0
94-95	5469.0
96-97	7238.0
98-99	9097.0
100-101	11168.0
102-103	13238.0
104-105	14851.0
106-107	16652.0
108-109	19174.0
110-111	20887.0
112-113	22458.0
114-115	24270.0
116-117	25780.0
118-119	27330.0
120-121	29674.0
122-123	31372.0
124-125	33165.0
126-127	35153.0
128-129	36957.0
130-131	39410.0
132-133	44623.0
134-135	124859.0
136-137	129964.0
138-139	127968.0
140-141	130298.0
142-143	135811.0
144-145	135945.0
146-147	134795.0
148-149	137443.0
150-151	144847.0
152-153	147761.0
154-155	136813.0
156-157	140630.0
158-159	144732.0
160-161	149190.0
162-163	158754.0
164-165	162941.0
166-167	168163.0
168-169	172966.0
170-171	177738.0
172-173	182389.0
174-175	189358.0
176-177	712407.0
178-179	6143584.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.33838605108394
#Duplication Level	Percentage of total
1	82.38605051280824
2	13.555514828613092
3	2.6642210672437088
4	0.7356157948925849
5	0.27488549331290446
6	0.11866327500696058
7	0.06167036627181297
8	0.06196409577124124
9	0.02801643498406422
>10	0.1121404682310631
>50	0.0012576628643293163
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2425625462974025E-4	0.0	0.0	2.867452029917083E-5	0.0	0.0
2	9.080264761404096E-4	9.558173433056943E-6	1.9116346866113887E-5	3.8232693732227774E-5	0.0	0.0
3	9.271428230065234E-4	2.867452029917083E-5	3.8232693732227774E-5	3.8232693732227774E-5	0.0	0.0
4	9.271428230065234E-4	2.867452029917083E-5	3.8232693732227774E-5	3.8232693732227774E-5	9.558173433056943E-6	0.0
5	9.271428230065234E-4	2.867452029917083E-5	3.8232693732227774E-5	4.779086716528472E-5	9.558173433056943E-6	0.0
6	9.462591698726373E-4	2.867452029917083E-5	3.8232693732227774E-5	4.779086716528472E-5	9.558173433056943E-6	0.0
7	9.462591698726373E-4	2.867452029917083E-5	3.8232693732227774E-5	4.779086716528472E-5	9.558173433056943E-6	0.0
8	9.749336901718081E-4	2.867452029917083E-5	3.8232693732227774E-5	5.734904059834166E-5	9.558173433056943E-6	0.0
9	9.749336901718081E-4	2.867452029917083E-5	3.8232693732227774E-5	5.734904059834166E-5	9.558173433056943E-6	0.0
10-14	9.768453248584195E-4	4.779086716528472E-5	4.396759779206193E-5	8.793519558412388E-5	1.529307749289111E-5	0.0
15-19	0.0010055198451575903	7.455375277784416E-5	7.646538746445555E-5	1.2999115868957443E-4	1.9116346866113887E-5	0.0
20-24	0.0010686037898157662	8.984683027073525E-5	8.602356089751249E-5	1.682238524218022E-4	1.9116346866113887E-5	0.0
25-29	0.001141245907906999	1.013166383904036E-4	1.1660971588329471E-4	2.1410308490047553E-4	2.676288561255944E-5	0.0
30-34	0.0019900117087624557	1.1469808119668333E-4	1.644005830485794E-4	2.733637601854286E-4	7.646538746445555E-5	0.0
35-39	0.005511242801500633	1.319027933761858E-4	1.7204712179502498E-4	2.982150111113766E-4	1.510191402422997E-4	3.058615498578222E-5
40-44	0.008543095414466295	1.663122177351908E-4	1.816052952280819E-4	3.498291476498841E-4	2.1601471958708693E-4	6.881884871800999E-5
45-49	0.010796912709981123	1.7204712179502498E-4	2.1027981552725273E-4	4.090898229348372E-4	2.8865683767831965E-4	1.013166383904036E-4
50-54	0.012546058448230544	1.816052952280819E-4	2.2557289302014386E-4	4.396759779206194E-4	3.59387321082941E-4	1.1469808119668333E-4
55-59	0.013832588592320009	1.9689837272097305E-4	2.466008745728691E-4	4.8555521039929264E-4	4.090898229348372E-4	1.2807952400296305E-4
60-64	0.01507515113861741	2.1983798896030968E-4	2.7718702955865134E-4	5.562856938039141E-4	4.492341513536763E-4	1.338144280627972E-4
65-69	0.016313890415541592	2.4086597051303495E-4	3.1159645391765637E-4	6.155463690888671E-4	5.084948266386293E-4	1.510191402422997E-4
70-74	0.03098950990465722	2.657172214389831E-4	3.383593395302158E-4	6.480441587612607E-4	6.117230997156444E-4	1.5866567898874525E-4
75-79	0.3248612870080528	2.905684723649311E-4	3.746803985758321E-4	7.034815646729909E-4	7.359793543453845E-4	1.6822385242180218E-4
80-84	0.4585457239121604	3.383593395302158E-4	3.861502066955005E-4	7.47449162465053E-4	9.978733064111448E-4	1.9116346866113887E-4
85-89	0.4594633085617339	3.8423857200888906E-4	4.090898229348372E-4	7.837702215106693E-4	0.0013610838968673086	1.9116346866113887E-4
90-94	0.45984372386436956	4.2629453511433963E-4	4.3585270854739656E-4	8.258261846161198E-4	0.0013763769743601998	1.9116346866113887E-4
95-99	0.46027575330354364	4.3967597792061937E-4	4.817319410260699E-4	8.755286864680159E-4	0.0013782886090468111	1.9116346866113887E-4
100-104	0.46075175034050997	4.4732251666706487E-4	4.989366532055725E-4	9.424359004994144E-4	0.0013859351477932566	1.9116346866113887E-4
105-109	0.46130230113025394	4.5879232478673334E-4	5.333460775645775E-4	9.921384023513107E-4	0.0013954933212263136	1.9116346866113887E-4
110-114	0.4617782981672203	4.6452722884656746E-4	5.562856938039141E-4	0.0010189012879638702	0.0013954933212263136	1.9116346866113887E-4
115-119	0.46235561184257684	4.8364357571268127E-4	5.773136753566394E-4	0.001057133981696098	0.0013954933212263136	1.9116346866113887E-4
120-124	0.46492102559200943	4.97025018518961E-4	5.983416569093645E-4	0.0011011015794881597	0.0014413725537049868	1.9116346866113887E-4
125-129	0.4703710960835384	5.008482878921838E-4	6.289278118951469E-4	0.0011622738894597243	0.0014738703433773807	1.9116346866113887E-4
130-134	0.4764711223685154	5.276111735047432E-4	6.595139668809291E-4	0.0012024182178785633	0.0014929866902434945	1.9498673803436164E-4
135-139	0.4832268393510001	5.524624244306914E-4	6.709837750005974E-4	0.0012425625462974025	0.0015178379411694425	2.1983798896030968E-4
140-144	0.4903954694257927	5.734904059834165E-4	6.977466606131568E-4	0.0012922650481492987	0.0015675404430213387	2.274845277067552E-4
145-149	0.4954307151903271	5.792253100432508E-4	7.03481564672991E-4	0.0013343210112547494	0.0015866567898874524	2.3513106645320077E-4
150-154	0.4997146885230233	5.849602141030849E-4	7.264211809123276E-4	0.0013687304356137541	0.0016019498673803436	2.3895433582642357E-4
155-159	0.5050194747783698	5.926067528495304E-4	7.340677196587731E-4	0.0013859351477932568	0.0016172429448732348	2.3895433582642357E-4
160-164	0.5113488972257402	6.002532915959761E-4	7.398026237186075E-4	0.0014203445721522616	0.0016344476570527371	2.3895433582642357E-4
165-167	0.5168869029128533	6.085370419046253E-4	7.550957012114985E-4	0.0014464702462026174	0.001644005830485794	2.4214039363744256E-4
>>END_MODULE
