##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479073
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10694092
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.14348006357155	39.0	38.0	39.0	37.0	39.0
2	39.82639807100968	41.0	40.0	41.0	38.0	41.0
3	39.86122178488833	41.0	40.0	41.0	38.0	41.0
4	39.855630192820485	41.0	40.0	41.0	38.0	41.0
5	39.84596448207103	41.0	40.0	41.0	38.0	41.0
6	39.820356604375576	41.0	40.0	41.0	38.0	41.0
7	39.831732885784035	41.0	40.0	41.0	38.0	41.0
8	39.79995234752048	41.0	40.0	41.0	38.0	41.0
9	39.76764217102303	41.0	40.0	41.0	38.0	41.0
10-14	39.66577061427937	40.0	39.2	41.0	37.2	41.0
15-19	39.59898729130065	40.0	39.0	41.0	37.0	41.0
20-24	39.58025668752429	40.0	39.0	41.0	37.0	41.0
25-29	39.394860676343534	40.0	39.0	41.0	36.2	41.0
30-34	39.098684619507665	40.0	38.0	41.0	35.2	41.0
35-39	38.6854795152314	40.0	37.6	41.0	34.8	41.0
40-44	38.22812844699672	39.4	36.4	41.0	34.0	41.0
45-49	37.86965025174648	39.0	35.2	40.8	34.0	41.0
50-54	37.26821106457659	37.4	35.0	40.0	34.0	41.0
55-59	36.59039645441614	36.2	35.0	39.2	33.0	41.0
60-64	35.692707655778534	35.0	34.6	37.6	32.4	39.8
65-69	35.34560763083018	35.0	34.6	36.8	32.8	39.0
70-74	35.20715342639656	35.0	34.8	36.0	33.0	38.6
75-79	35.02621522238634	35.0	34.0	35.6	33.0	38.0
80-84	34.96123468920971	35.0	34.0	35.0	33.0	38.0
85-89	34.693288817788364	34.8	33.6	35.0	32.2	39.0
90-94	39.3054064954866	40.4	38.8	40.6	36.8	40.8
95-99	39.47710210153857	40.0	39.0	41.0	36.2	41.0
100-104	39.27902270233025	40.0	38.6	41.0	36.0	41.0
105-109	39.27242542327517	40.0	38.8	41.0	35.2	41.0
110-114	39.110910739523874	40.0	38.0	41.0	35.0	41.0
115-119	38.86242385675263	40.0	38.0	41.0	35.0	41.0
120-124	38.54359186760779	40.0	37.2	41.0	34.4	41.0
125-129	37.95432633591588	39.4	36.2	40.4	33.8	40.6
130-134	38.00914136860172	39.0	35.4	40.8	34.0	41.0
135-139	37.57644455918075	38.2	35.0	40.8	34.0	41.0
140-144	36.94994664223197	36.8	35.0	39.6	33.8	41.0
145-149	36.27770772986503	35.6	35.0	38.8	33.0	40.6
150-154	35.615080518198326	35.0	35.0	37.0	33.0	39.2
155-159	35.067797414467655	35.0	34.8	36.0	33.0	37.6
160-164	34.67810337893142	35.0	34.0	35.2	33.0	36.4
165-169	34.4288979567693	35.0	34.0	35.0	33.0	36.0
170-174	34.26823619617038	35.0	34.0	35.0	32.0	35.6
175-178	33.74293345530419	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	5.0
30	276.0
31	3592.0
32	24970.0
33	110970.0
34	346717.0
35	890024.0
36	2030143.0
37	3379462.0
38	3218851.0
39	685517.0
40	3565.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.291017507610743	27.418943094935035	29.187349426206545	22.102689971247678
2	22.280414269860405	28.204058839217016	29.33667486683301	20.178852024089565
3	21.01424786695308	26.960512402548996	29.697930408677987	22.327309321819936
4	21.238465126352008	26.830290968134555	30.36654257322641	21.564701332287022
5	22.230208978939025	27.23555211606558	28.75612066924429	21.778118235751105
6	22.708426297436006	27.03932227252206	28.014440122639677	22.237811307402254
7	22.355259333845268	27.943213879214806	28.085862736172455	21.61566405076747
8	22.330011748543026	27.957474089431813	28.11864719323529	21.59386696878987
9	22.910593999004313	27.636829756093363	27.34402322329002	22.108553021612305
10-14	22.36994033715064	28.019594370424343	27.743503609282584	21.86696168314243
15-19	22.310917093288516	27.993086276048494	27.72817926009988	21.96781737056311
20-24	22.147855096066127	27.965267177428434	27.854456460632655	22.032421265872784
25-29	22.036043826815778	28.117624198482677	27.7924689632369	22.053863011464646
30-34	22.08127253814536	28.111983700907007	27.722441512566004	22.08430224838163
35-39	21.97790331334348	28.081056344007514	27.760610250968476	22.180430091680527
40-44	21.99156132189624	28.178607403040857	27.750255000611553	22.07957627445135
45-49	22.321044180281973	27.98293300637399	27.540754278156577	22.155268535187467
50-54	22.237592494996303	28.00379499259965	27.63889070713063	22.119721805273414
55-59	22.148212302643365	28.060549694167587	27.666917397007616	22.12432060618143
60-64	22.181067826983348	28.05101358768935	27.62671763063194	22.141200954695357
65-69	22.10805181028927	28.022562364340985	27.65541197887581	22.213973846493936
70-74	21.953295333535564	28.249280069780585	27.59588752369065	22.2015370729932
75-79	22.02912972882597	28.258993844451684	27.43307800232128	22.27879842440106
80-84	22.046243851277882	28.36267539123471	27.30967528612995	22.281405471357456
85-89	22.09382339332783	28.497789246623277	27.172158234658912	22.23622912538998
90-94	21.53158592112599	27.239961455160294	29.34692160831458	21.881531015399137
95-99	22.220822628737334	27.669397615650794	28.072647217024155	22.03713253858772
100-104	22.21330478920466	27.735962579869728	27.904505067387525	22.146227563538087
105-109	21.94721243946137	27.86237509081937	28.131011871044624	22.05940059867464
110-114	21.987958338658235	27.73658992590728	28.066129533196595	22.209322202237892
115-119	21.945529924943383	27.79903813884765	28.008651125986262	22.24678081022271
120-124	21.82982226402728	27.898928167638022	28.088244166557015	22.183005401777685
125-129	21.88088533074631	27.89462605707824	28.03281506343555	22.191673548739892
130-134	21.928732182782767	27.878620406618204	28.036545553066965	22.156101857532065
135-139	21.892306798033545	27.97649681877311	28.067076512661842	22.0641198705315
140-144	21.90136864806047	28.02388982699059	28.076397637241314	21.998343887707627
145-149	21.7932615977132	28.05382500357339	28.170602810384608	21.982310588328797
150-154	21.6057167025989	28.273996653263634	28.330132638201906	21.790154005935563
155-159	21.55585935117144	28.347071812527787	28.27282071660717	21.824248119693607
160-164	21.411410221900066	28.57985325140948	28.27231594672964	21.736420579960807
165-169	21.229003053465892	28.86117108313201	28.343230109647415	21.566595753754687
170-174	21.30001214226466	29.13176748611448	28.167284535191882	21.400935836428978
175-178	21.28542309874994	29.353768340295456	28.11800208140624	21.242806479548364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	3.5
8	11.5
9	20.5
10	38.5
11	93.0
12	212.5
13	440.0
14	767.5
15	1314.5
16	2157.5
17	3431.0
18	5638.0
19	8697.0
20	12928.5
21	18753.5
22	26408.5
23	36298.0
24	48542.5
25	63165.0
26	80539.5
27	100905.0
28	122937.5
29	146722.0
30	172058.0
31	198076.0
32	225054.5
33	252306.0
34	279334.5
35	304864.5
36	328835.5
37	350859.5
38	369496.5
39	385686.5
40	398791.0
41	407802.5
42	412617.0
43	413520.5
44	409283.5
45	399050.0
46	385555.0
47	369611.5
48	351124.0
49	329831.5
50	307528.0
51	285215.5
52	263126.0
53	242793.5
54	223458.0
55	206185.0
56	191676.5
57	178950.0
58	167246.0
59	156454.5
60	146534.5
61	136457.5
62	125280.5
63	113387.0
64	101293.0
65	88778.0
66	76008.5
67	63522.5
68	51550.5
69	40630.5
70	31251.5
71	23387.5
72	16954.0
73	11891.5
74	8060.0
75	5278.0
76	3453.0
77	2175.5
78	1286.5
79	751.5
80	422.0
81	237.0
82	129.0
83	68.0
84	39.5
85	18.0
86	4.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1596.0
92-93	3685.0
94-95	5637.0
96-97	7291.0
98-99	9622.0
100-101	11541.0
102-103	13261.0
104-105	14711.0
106-107	16781.0
108-109	18986.0
110-111	20707.0
112-113	22257.0
114-115	23692.0
116-117	25433.0
118-119	27242.0
120-121	29121.0
122-123	30809.0
124-125	32775.0
126-127	34584.0
128-129	36229.0
130-131	38080.0
132-133	42983.0
134-135	131857.0
136-137	130859.0
138-139	128634.0
140-141	133104.0
142-143	138719.0
144-145	137005.0
146-147	132659.0
148-149	133435.0
150-151	141147.0
152-153	144186.0
154-155	135977.0
156-157	139332.0
158-159	146716.0
160-161	150226.0
162-163	158386.0
164-165	163670.0
166-167	166588.0
168-169	174511.0
170-171	180158.0
172-173	181662.0
174-175	194378.0
176-177	658213.0
178-179	6425647.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.37710293974666
#Duplication Level	Percentage of total
1	82.50308164509791
2	13.418657705871977
3	2.6102368106657563
4	0.7171550587007941
5	0.3012314170776205
6	0.13291605653488703
7	0.07909465916359694
8	0.057791450617335434
9	0.037324500252246935
>10	0.13980771578016513
>50	0.0027029802376627413
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.61057451160884E-5	0.0	9.350957519348067E-6	1.8701915038696133E-5	0.0	0.0
2	7.293746865091492E-4	0.0	1.8701915038696133E-5	1.8701915038696133E-5	0.0	0.0
3	7.480766015478453E-4	0.0	1.8701915038696133E-5	1.8701915038696133E-5	0.0	0.0
4	7.574275590671933E-4	0.0	1.8701915038696133E-5	1.8701915038696133E-5	0.0	0.0
5	7.574275590671933E-4	0.0	1.8701915038696133E-5	3.740383007739227E-5	0.0	0.0
6	7.574275590671933E-4	9.350957519348067E-6	2.80528725580442E-5	3.740383007739227E-5	0.0	0.0
7	7.667785165865414E-4	9.350957519348067E-6	2.80528725580442E-5	4.675478759674033E-5	9.350957519348067E-6	0.0
8	7.667785165865414E-4	9.350957519348067E-6	3.740383007739227E-5	4.675478759674033E-5	9.350957519348067E-6	0.0
9	7.667785165865414E-4	9.350957519348067E-6	3.740383007739227E-5	5.61057451160884E-5	9.350957519348067E-6	0.0
10-14	7.779996656097591E-4	9.350957519348067E-6	3.740383007739227E-5	7.480766015478455E-5	1.3091340527087292E-5	0.0
15-19	8.116631126794121E-4	1.683172353482652E-5	4.675478759674033E-5	1.1034129872830719E-4	3.740383007739227E-5	0.0
20-24	8.602880917800221E-4	1.8701915038696133E-5	5.61057451160884E-5	1.3278359677474256E-4	3.740383007739227E-5	0.0
25-29	9.238746029115889E-4	2.8052872558044202E-5	8.789900068187183E-5	1.570960863250475E-4	4.114421308513149E-5	0.0
30-34	0.0018047348012341768	4.4884596092870715E-5	1.0286053271282872E-4	2.0946144843339668E-4	6.732689413930608E-5	0.0
35-39	0.00580694461951515	5.797593661995801E-5	1.5709608632504752E-4	2.2816336347209281E-4	1.1595187323991602E-4	1.1221149023217679E-5
40-44	0.009416414221983502	7.667785165865415E-5	1.9449991640243977E-4	2.880094915959204E-4	1.6083646933278675E-4	6.171631962769724E-5
45-49	0.011733581495277954	1.1034129872830717E-4	2.3564412948757127E-4	3.5159600272748726E-4	1.98240299410179E-4	1.122114902321768E-4
50-54	0.013472859593876693	1.3278359677474256E-4	2.7304795956496357E-4	4.0396136483583645E-4	2.431248955030497E-4	1.3465378827861216E-4
55-59	0.015122368500289692	1.421345542940906E-4	2.8052872558044197E-4	4.5071615243257675E-4	2.992306406191381E-4	1.533557033173083E-4
60-64	0.016710161087074998	1.7018742685213478E-4	2.8987968309979005E-4	4.937305570215779E-4	3.6281715175070497E-4	1.720576183560044E-4
65-69	0.01848310263274339	1.8140857587535249E-4	3.0858159813848616E-4	5.629276426647536E-4	4.320142373938807E-4	1.8514895888309169E-4
70-74	0.034022523838396004	1.8888934189083094E-4	3.235431301694431E-4	6.339949198117988E-4	5.124324720602741E-4	1.870191503869613E-4
75-79	0.33827275845392013	2.001104909140486E-4	3.272835131771823E-4	6.788795159046695E-4	5.722786001841017E-4	1.870191503869613E-4
80-84	0.4772803525535408	2.169422144488751E-4	3.441152367120088E-4	7.069323884627138E-4	8.210140701987601E-4	1.870191503869613E-4
85-89	0.4780864050917086	2.375143209914409E-4	3.6094696024683534E-4	7.312448780130188E-4	0.0012137542860113791	1.870191503869613E-4
90-94	0.47847540492451346	2.580864275340066E-4	3.8525944979714034E-4	7.817400486174984E-4	0.001238066775561684	1.870191503869613E-4
95-99	0.47893921241747306	2.880094915959204E-4	4.020911733319668E-4	8.247544532064995E-4	0.0012679898396235976	1.870191503869613E-4
100-104	0.4793506545483245	3.1793255565783424E-4	4.3575462040161984E-4	8.621582832838917E-4	0.0012754706056390763	1.870191503869613E-4
105-109	0.47978266878571835	3.2541332167331275E-4	4.750286419828818E-4	8.920813473458055E-4	0.0012885619461661636	1.870191503869613E-4
110-114	0.4803343752793599	3.4972581122361763E-4	4.862497910060995E-4	9.425765179502851E-4	0.0013053936697009904	1.870191503869613E-4
115-119	0.4809964230717297	3.7964887528553145E-4	5.311343870989702E-4	9.743697735160685E-4	0.0013259657762435559	1.870191503869613E-4
120-124	0.48351744121894596	4.0583155633970607E-4	5.573170681531447E-4	0.001000552454570243	0.0013427974997783825	1.870191503869613E-4
125-129	0.488724054365719	4.320142373938806E-4	5.797593661995801E-4	0.0010791004977327668	0.001372720563840296	1.9075953339470054E-4
130-134	0.4945927153048618	4.488459609287072E-4	6.00331472742146E-4	0.0011688696899185085	0.0013951628618867314	2.0572106542565745E-4
135-139	0.5010710586742662	4.488459609287072E-4	6.396054943234077E-4	0.0011969225624765525	0.0014176051599331667	2.0946144843339668E-4
140-144	0.5074016569148647	4.638074929596641E-4	6.583074093621038E-4	0.0012418071585694232	0.001460619564522168	2.1507202294500553E-4
145-149	0.5121145395046163	4.750286419828818E-4	6.844900904162784E-4	0.0012754706056390761	0.0014868022455763424	2.1507202294500553E-4
150-154	0.5164646049426169	4.806392164944906E-4	7.181535374859314E-4	0.0013240955847396863	0.0014998935861034297	2.1507202294500553E-4
155-159	0.5216487757913435	4.862497910060995E-4	7.387256440284973E-4	0.0013596292233132089	0.001527946458661474	2.2255278896048396E-4
160-164	0.5276464799442533	4.862497910060995E-4	7.705188995942807E-4	0.0013951628618867314	0.001567220480242736	2.244229804643536E-4
165-167	0.5329671747727625	4.8624979100609945E-4	7.948313891445857E-4	0.0014057606137419926	0.0015990137358085192	2.30656952143919E-4
>>END_MODULE
