##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479074
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10551225
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.217598525289716	39.0	39.0	39.0	37.0	39.0
2	39.91357458494156	41.0	40.0	41.0	38.0	41.0
3	39.92640807110075	41.0	40.0	41.0	38.0	41.0
4	39.91727851505394	41.0	40.0	41.0	38.0	41.0
5	39.907632526081095	41.0	40.0	41.0	38.0	41.0
6	39.88186442806404	41.0	40.0	41.0	38.0	41.0
7	39.88387954953098	41.0	40.0	41.0	38.0	41.0
8	39.837842051515345	41.0	40.0	41.0	38.0	41.0
9	39.80656397716853	41.0	40.0	41.0	38.0	41.0
10-14	39.67527808382439	40.0	39.4	41.0	37.4	41.0
15-19	39.61530536975564	40.0	39.4	41.0	37.0	41.0
20-24	39.64543135038822	40.0	39.4	41.0	37.0	41.0
25-29	39.44529328111191	40.0	39.0	41.0	36.4	41.0
30-34	39.12652618060936	40.0	38.2	41.0	35.2	41.0
35-39	38.690058187556424	40.0	37.6	41.0	34.8	41.0
40-44	38.20438256221434	39.4	36.2	41.0	34.0	41.0
45-49	37.82271491698831	38.8	35.2	40.6	34.0	41.0
50-54	37.19321989626796	37.4	35.0	40.0	33.8	41.0
55-59	36.497804662491795	36.2	35.0	39.0	33.0	40.8
60-64	35.5568713016735	35.0	34.4	37.6	32.4	39.8
65-69	35.2218772701748	35.0	33.8	36.4	32.4	39.0
70-74	35.13036270196115	35.0	34.0	36.0	33.0	39.0
75-79	34.96507438709724	35.0	34.0	35.6	32.8	38.4
80-84	34.920869207129975	35.0	34.0	35.0	32.0	39.0
85-89	34.66156877518961	34.8	33.4	35.4	31.0	39.8
90-94	39.37821899772475	40.6	38.8	40.6	36.8	41.0
95-99	39.48804687998895	40.0	39.0	41.0	36.4	41.0
100-104	39.26033996620613	40.0	38.4	41.0	35.8	41.0
105-109	39.30135619695055	40.0	39.0	41.0	35.4	41.0
110-114	39.14322209252734	40.0	38.0	41.0	35.0	41.0
115-119	38.89317024582239	40.0	38.0	41.0	35.0	41.0
120-124	38.57178482867959	40.0	37.2	41.0	34.6	41.0
125-129	37.96346823224943	39.4	36.2	40.4	33.8	40.6
130-134	38.041224974478325	39.0	35.4	40.8	34.0	41.0
135-139	37.60547765100542	38.2	35.0	40.8	34.0	41.0
140-144	36.99185793570213	36.8	35.0	39.8	34.0	41.0
145-149	36.32617445049651	35.6	35.0	38.8	33.0	40.8
150-154	35.665568260874714	35.0	35.0	37.0	33.0	39.2
155-159	35.13120586819883	35.0	35.0	36.0	33.0	37.6
160-164	34.72869880853685	35.0	34.2	35.2	33.0	36.6
165-169	34.4717350333929	35.0	34.0	35.0	33.0	36.0
170-174	34.3111547493172	35.0	34.0	35.0	32.8	35.8
175-178	33.66666809001594	34.5	33.25	34.75	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	9.0
30	210.0
31	2804.0
32	20686.0
33	93038.0
34	306759.0
35	840979.0
36	2027244.0
37	3464314.0
38	3188113.0
39	604384.0
40	2685.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.39125077893799	27.351544488910058	29.06450198910553	22.192702743046425
2	22.374520494065855	28.159554933194965	29.251437629280012	20.21448694345917
3	21.06449251153302	26.911406021575697	29.62160317877782	22.402498288113463
4	21.28346234678912	26.75657091949039	30.334231333328592	21.6257354003919
5	22.27644657373907	27.16573667986419	28.700079848548395	21.857736897848355
6	22.785818708254254	26.947307066241123	27.967292897270223	22.2995813282344
7	22.409852884380722	27.87133247561302	28.03782499188483	21.680989648121425
8	22.372226921518592	27.902362048008644	28.05641998914818	21.66899104132459
9	22.95277562557902	27.547313226663256	27.27744882703193	22.222462320725793
10-14	22.41837132655213	27.967751611779672	27.688836130401924	21.925040931266274
15-19	22.372352025475713	27.922386263206405	27.674471921506743	22.030789789811138
20-24	22.212273930278236	27.87910218955619	27.80968465746868	22.098939222696888
25-29	22.07750664022424	28.068949340005545	27.75298602768873	22.100557992081487
30-34	22.129838004591885	28.038056244654058	27.673173494072962	22.158932256681098
35-39	22.022197422574155	28.0291833412708	27.707741992043577	22.240877244111466
40-44	22.032869169219687	28.12262651966952	27.71017204163498	22.13433226947582
45-49	22.370992941577875	27.929437577153365	27.476453208039825	22.22311627322894
50-54	22.275337697755475	27.934309049423174	27.589535812192423	22.20081744062893
55-59	22.186574544661873	28.008103324495497	27.635483083717766	22.16983904712486
60-64	22.200948231129562	27.97039964553879	27.5951863409225	22.23346578240915
65-69	22.13391715179991	27.947232667296923	27.63289001987921	22.285960161023958
70-74	21.97257285291518	28.172440640778678	27.56785112629102	22.287135380015116
75-79	22.037981371831233	28.17643638534862	27.429544910662035	22.356037332158117
80-84	22.042849053072036	28.26468774952671	27.3397354335634	22.352727763837848
85-89	22.08093941698713	28.363279145312507	27.22323142573493	22.332550011965434
90-94	21.635318497563656	27.184561621617593	29.23609311768484	21.94402676313391
95-99	22.275997048267087	27.61912631977852	28.016968004110304	22.087908627844087
100-104	22.26003462517732	27.67864472597531	27.851321725415723	22.20999892343164
105-109	22.025382066997636	27.796509748242954	28.061660689462293	22.116447495297116
110-114	22.040530818247902	27.679936547810545	28.0119974598729	22.267535174068655
115-119	22.002594160719426	27.74941475260016	27.94569705950466	22.302294027175755
120-124	21.903396683052332	27.836352950602073	28.021160990089417	22.239089376256178
125-129	21.932337110241132	27.82590410038459	27.976874018349363	22.264884771024914
130-134	21.988624553975306	27.826977639379557	27.97012921764994	22.214268588995193
135-139	21.94120181625475	27.918078988579488	28.024049473419506	22.11666972174626
140-144	21.931917319633346	27.988690070362885	28.04712506863931	22.032267541364458
145-149	21.834405367370817	28.03582546822746	28.123050521148603	22.006718643253123
150-154	21.639703517340052	28.24008290569796	28.302195596920328	21.818017980041656
155-159	21.57118765165775	28.31994050424686	28.272254697503378	21.83661714659201
160-164	21.445658255453353	28.573762546139747	28.25028788785577	21.730291310551138
165-169	21.256365754198953	28.87906425336151	28.309154995998604	21.555414996440934
170-174	21.322992439878576	29.163360769420173	28.152107030651297	21.361539760049965
175-178	21.326266659523668	29.40364032127738	28.077719340046603	21.192373679152347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.5
7	5.5
8	10.0
9	21.0
10	42.0
11	93.5
12	194.0
13	392.0
14	751.5
15	1255.5
16	2055.5
17	3425.5
18	5382.5
19	8222.5
20	12404.0
21	18064.5
22	25291.5
23	34512.0
24	46420.0
25	60982.0
26	78281.5
27	97983.0
28	119142.0
29	141866.5
30	166930.0
31	192921.5
32	219256.0
33	245603.0
34	271526.0
35	297397.0
36	321477.5
37	343079.5
38	362174.0
39	378747.5
40	391966.5
41	401043.5
42	406236.0
43	406809.5
44	403039.0
45	394800.0
46	382406.5
47	366496.0
48	347900.0
49	327712.5
50	305676.5
51	283321.5
52	262194.5
53	241876.5
54	222631.5
55	206568.0
56	192255.5
57	179125.5
58	167823.5
59	157198.5
60	147025.0
61	136244.0
62	125098.5
63	113620.5
64	101095.5
65	88676.5
66	76148.5
67	63249.5
68	51436.5
69	40795.0
70	31369.5
71	23448.0
72	16919.0
73	11838.5
74	8115.0
75	5414.5
76	3512.0
77	2209.5
78	1331.0
79	792.0
80	442.5
81	234.0
82	127.5
83	64.5
84	31.5
85	17.5
86	8.5
87	4.5
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1375.0
92-93	3248.0
94-95	5168.0
96-97	6851.0
98-99	8663.0
100-101	10584.0
102-103	12371.0
104-105	13997.0
106-107	16131.0
108-109	18583.0
110-111	19915.0
112-113	21854.0
114-115	23334.0
116-117	25037.0
118-119	27217.0
120-121	29146.0
122-123	30707.0
124-125	33012.0
126-127	35157.0
128-129	37518.0
130-131	39504.0
132-133	46233.0
134-135	122416.0
136-137	127128.0
138-139	124877.0
140-141	128821.0
142-143	133141.0
144-145	134347.0
146-147	134880.0
148-149	139130.0
150-151	148272.0
152-153	151340.0
154-155	139534.0
156-157	143959.0
158-159	151702.0
160-161	155558.0
162-163	165815.0
164-165	172986.0
166-167	176344.0
168-169	185733.0
170-171	193831.0
172-173	200028.0
174-175	221682.0
176-177	1126305.0
178-179	5707791.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.36343434854679
#Duplication Level	Percentage of total
1	82.43517054874523
2	13.578087313182383
3	2.523857162872187
4	0.7324905897871299
5	0.30436273473196646
6	0.14318633376096138
7	0.0760422931655924
8	0.05265508536264051
9	0.04045555091924209
>10	0.11369238747268161
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.0425329760288497E-4	1.8955145018706357E-5	0.0	9.477572509353179E-6	0.0	0.0
2	7.866385182763139E-4	2.843271752805954E-5	0.0	3.7910290037412715E-5	0.0	0.0
3	8.150712358043734E-4	4.73878625467659E-5	0.0	3.7910290037412715E-5	0.0	0.0
4	8.150712358043734E-4	5.686543505611908E-5	0.0	3.7910290037412715E-5	0.0	0.0
5	8.245488083137266E-4	5.686543505611908E-5	1.8955145018706357E-5	4.73878625467659E-5	0.0	0.0
6	8.340263808230797E-4	5.686543505611908E-5	1.8955145018706357E-5	6.634300756547225E-5	0.0	0.0
7	8.43503953332433E-4	5.686543505611908E-5	1.8955145018706357E-5	9.47757250935318E-5	0.0	0.0
8	8.529815258417862E-4	5.686543505611908E-5	2.843271752805954E-5	9.47757250935318E-5	0.0	0.0
9	8.529815258417862E-4	5.686543505611908E-5	4.73878625467659E-5	9.47757250935318E-5	0.0	0.0
10-14	8.624590983511394E-4	7.582058007482543E-5	6.255197856173099E-5	1.0235778310101434E-4	0.0	0.0
15-19	8.852052723735869E-4	8.529815258417862E-5	1.1183535561036752E-4	1.326860151309445E-4	1.5164116014965087E-5	0.0
20-24	9.136379899016466E-4	9.47757250935318E-5	1.4595461664403895E-4	1.8386490668145165E-4	1.8955145018706357E-5	0.0
25-29	9.667123959540242E-4	1.0425329760288498E-4	1.7628284867396917E-4	2.1798416771512315E-4	3.222374653180081E-5	0.0
30-34	0.0019030965598781184	1.2510395712346197E-4	1.857604211833223E-4	2.5778997225440645E-4	4.170131904115399E-5	1.8955145018706358E-6
35-39	0.005678961447604425	1.4405910214216832E-4	2.160886532132525E-4	2.900137187862073E-4	6.634300756547225E-5	2.6537203026188902E-5
40-44	0.008836888607720906	1.6870079066648658E-4	2.5020791424692395E-4	3.6393878435916216E-4	1.2699947162533257E-4	4.73878625467659E-5
45-49	0.010948491762804793	1.7059630516835724E-4	2.6916305926563027E-4	4.3028179192463434E-4	1.7249181967022786E-4	5.686543505611908E-5
50-54	0.01248575402382188	1.7438733417209852E-4	2.843271752805954E-4	4.568189949508233E-4	2.312527692282176E-4	7.013403656921352E-5
55-59	0.013786076972105132	1.9523799369267552E-4	3.032823202993017E-4	4.795651689732709E-4	2.65372030261889E-4	8.150712358043734E-5
60-64	0.015162220500463217	2.2177519671886438E-4	3.449836393404558E-4	5.117889155050717E-4	2.994912912955605E-4	9.856675409727306E-5
65-69	0.016396200441181	2.4641688524318264E-4	3.866849583816097E-4	5.781319230705439E-4	3.601477553554208E-4	1.0993984110849688E-4
70-74	0.032079687429658645	2.5589445775253585E-4	3.9616253089096284E-4	6.141466986060859E-4	4.283862774227637E-4	1.1941741361785004E-4
75-79	0.3450272361739988	2.900137187862073E-4	3.980580453928335E-4	6.596390466509812E-4	5.421171475350018E-4	1.269994716253326E-4
80-84	0.48435892514850165	3.089688638049136E-4	3.980580453928335E-4	6.880717641790409E-4	7.563102862463836E-4	1.326860151309445E-4
85-89	0.48510575786223875	3.336105523292319E-4	4.189087049134105E-4	7.127134527033591E-4	0.001076652237062521	1.326860151309445E-4
90-94	0.48553983068316714	3.563567263516796E-4	4.283862774227637E-4	7.733699167632195E-4	0.0010899208385756157	1.326860151309445E-4
95-99	0.48601370930863474	3.8099841487599783E-4	4.416548789358581E-4	8.245488083137265E-4	0.0010899208385756157	1.326860151309445E-4
100-104	0.4865482443981623	3.980580453928335E-4	4.719831109657884E-4	8.624590983511394E-4	0.0010975028965830983	1.326860151309445E-4
105-109	0.4870429736831506	3.980580453928335E-4	5.023113429957185E-4	9.231155624109998E-4	0.001108875983594322	1.326860151309445E-4
110-114	0.4876874486137865	4.11326646905928E-4	5.30744060523778E-4	9.989361424858252E-4	0.0011183535561036751	1.326860151309445E-4
115-119	0.48834898317493936	4.53027965947082E-4	5.459081765387432E-4	0.0010804432660662625	0.0011183535561036751	1.326860151309445E-4
120-124	0.49084158474489936	4.7766965447140015E-4	5.762364085686734E-4	0.001114562527099934	0.0011259356141111578	1.326860151309445E-4
125-129	0.4960788913135678	5.09893401003201E-4	6.065646405986034E-4	0.0011524728171373468	0.0011373087011223816	1.4026807313842704E-4
130-134	0.5021938210966026	5.421171475350018E-4	6.425794161341455E-4	0.0012036517086878537	0.0011429952446279936	1.5164116014965086E-4
135-139	0.5098858189451936	5.705498650630614E-4	6.482659596397575E-4	0.0012396664842233957	0.0011733234766579236	1.7059630516835724E-4
140-144	0.5174735634961817	5.876094955798971E-4	6.672211046584638E-4	0.001260517143743973	0.0011903831071747595	1.7817836317583977E-4
145-149	0.5233515539664826	5.970870680892503E-4	6.804897061715583E-4	0.001285158832268291	0.0012036517086878539	1.800738776777104E-4
150-154	0.5285225175275856	5.970870680892503E-4	7.089224236996178E-4	0.0013098005207926094	0.00122260685370656	1.857604211833223E-4
155-159	0.5349748488919533	6.160422131079567E-4	7.221910252127123E-4	0.0013609794123431167	0.001254830600238361	1.895514501870636E-4
160-164	0.5423370272172189	6.255197856173099E-4	7.335641122239361E-4	0.0013969941878786587	0.001285158832268291	1.895514501870636E-4
165-167	0.5494433110847319	6.255197856173099E-4	7.392506557295479E-4	0.0014121583038936238	0.0012984274337813856	1.895514501870636E-4
>>END_MODULE
