##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479093
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11054168
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.89319386135619	41.0	40.0	41.0	38.0	41.0
2	39.96777695073931	41.0	40.0	41.0	38.0	41.0
3	40.013364823114685	41.0	40.0	41.0	38.0	41.0
4	40.00296422127835	41.0	40.0	41.0	38.0	41.0
5	39.98125141575558	41.0	40.0	41.0	38.0	41.0
6	39.94892659492781	41.0	40.0	41.0	38.0	41.0
7	39.91850160048228	41.0	40.0	41.0	38.0	41.0
8	39.89953888886075	41.0	40.0	41.0	38.0	41.0
9	39.85117803528949	41.0	40.0	41.0	38.0	41.0
10-14	39.79684044968377	40.6	40.0	41.0	38.0	41.0
15-19	39.79825911818963	41.0	40.0	41.0	37.6	41.0
20-24	39.566143069292956	40.4	39.0	41.0	36.6	41.0
25-29	39.16983295350677	40.0	38.4	41.0	35.2	41.0
30-34	38.6092646321279	40.0	37.0	41.0	35.0	41.0
35-39	37.93353279957388	39.0	35.4	41.0	34.0	41.0
40-44	37.426396342085624	37.8	35.0	40.4	34.0	41.0
45-49	36.76647300819021	36.4	35.0	39.6	33.0	41.0
50-54	36.1964529216491	35.2	35.0	38.8	33.0	41.0
55-59	35.7066915212434	35.0	35.0	37.2	33.0	40.0
60-64	34.89637454397292	34.8	33.6	35.8	32.0	39.0
65-69	35.16846671771226	35.0	34.8	36.0	33.0	39.0
70-74	35.021191264688575	35.0	34.0	35.2	33.0	39.0
75-79	34.974915543168876	35.0	34.0	35.0	33.0	39.0
80-84	34.99840516264995	35.0	34.0	35.0	32.4	39.8
85-89	38.42336939333652	39.6	37.4	39.8	35.0	40.8
90-94	39.52102989465572	40.6	39.0	41.0	36.2	41.0
95-99	39.309925369670076	40.0	38.8	41.0	35.6	41.0
100-104	39.235313481586395	40.0	38.6	41.0	35.0	41.0
105-109	39.022106204864485	40.0	38.0	41.0	35.0	41.0
110-114	38.69713705241164	40.0	37.6	41.0	34.8	41.0
115-119	38.259685497028435	39.8	36.0	41.0	34.0	41.0
120-124	37.51334826080942	38.4	35.0	40.4	33.6	40.6
125-129	37.45056851050257	38.2	35.0	40.8	33.6	41.0
130-134	36.941335752950934	36.6	35.0	40.0	33.0	41.0
135-139	36.36304717781697	35.4	35.0	39.2	33.0	41.0
140-144	35.81745139245602	35.0	35.0	37.6	33.0	40.4
145-149	35.30308008966426	35.0	35.0	36.4	33.0	39.0
150-154	34.885730568249954	35.0	34.2	35.6	33.0	37.2
155-159	34.55795515993956	35.0	34.0	35.0	32.8	36.4
160-164	34.337503765113716	35.0	34.0	35.0	32.0	36.0
165-169	34.17896504697623	35.0	34.0	35.0	32.0	35.4
170-173	33.6583924918056	34.75	33.25	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	11.0
30	242.0
31	3401.0
32	23908.0
33	114308.0
34	398110.0
35	1192359.0
36	2812193.0
37	3360758.0
38	2488762.0
39	649604.0
40	10512.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.9591527829141	25.48232485701321	26.656271191101855	23.902251168970835
2	23.889785282800116	25.761911706064172	26.545724653361518	23.80257835777419
3	23.56782527640253	26.054959541052753	26.490614218998658	23.88660096354606
4	23.959406081036583	26.01064141597993	26.0753771789971	23.95457532398639
5	24.256298619669973	26.13904547135524	25.79484046198683	23.809815446987958
6	23.617435522962925	26.339883743398868	26.091470656136217	23.95121007750199
7	23.898777366148224	26.157301028896974	25.991625964070746	23.952295640884053
8	24.309445993583598	26.07870624003543	25.78931313509981	23.82253463128116
9	23.650698994261713	26.189071850545425	26.026970098518497	24.13325905667437
10-14	23.96557569959132	26.080233265859537	25.979478509825434	23.974712524723707
15-19	23.833732217567167	26.003986912447868	26.111730887390166	24.050549982594802
20-24	23.930242420777393	25.996796864313986	25.973717786811275	24.099242928097347
25-29	23.968711168493186	26.034816912498528	25.886552475048326	24.109919443959964
30-34	23.807206476326396	26.01885732150986	25.987091927678318	24.186844274485424
35-39	23.898402846781412	26.027453174223513	25.89612533480584	24.178018644189233
40-44	24.06771635820986	25.895063291963723	25.78069557111851	24.256524778707906
45-49	24.155411786757718	25.895539130579525	25.719268967144338	24.229780115518416
50-54	24.18206598633203	25.882586550159182	25.703955286368004	24.231392177140783
55-59	24.159777560825926	25.919515607144746	25.664534861420595	24.256171970608733
60-64	24.015833665636347	25.91428319164319	25.78823480880696	24.281648333913505
65-69	24.04607203364378	25.984748920045362	25.74262305403717	24.22655599227368
70-74	24.054137769572527	25.998099540372465	25.69095747414007	24.25680521591494
75-79	23.991589416770218	26.025424979971355	25.695207454780856	24.28777814847757
80-84	24.05153422672787	26.067494179571	25.623862420039213	24.257109173661917
85-89	23.351606380507334	25.780465793535978	26.936844093558193	23.931083732398495
90-94	23.716135421469097	26.142436501506122	26.087060381469808	24.054367695554976
95-99	23.816803200863752	26.139837603001425	25.940622711635736	24.102736484499083
100-104	23.712632944914755	26.111568491354532	26.06311219693278	24.112686366797938
105-109	23.78965709042868	26.00515554544836	26.03385223625143	24.171335127871526
110-114	23.860743814167435	26.0447302145196	25.875388438774984	24.21913753253798
115-119	23.785421506155473	26.06439339449737	25.905419147089674	24.24476595225748
120-124	23.843519262799113	26.06381051983835	25.933388257726577	24.159281959635965
125-129	23.92933316624498	26.045769512577806	25.862446863622	24.162450457555217
130-134	23.949299599634337	26.055261902520265	25.857432309602697	24.1380061882427
135-139	23.92185579027243	26.186849967535526	25.906572447509497	23.98472179468255
140-144	23.812860445435046	26.28619789359316	25.984098219194045	23.91684344177775
145-149	23.61664003297524	26.45432923176516	26.133147453056978	23.795883282202624
150-154	23.50379882149623	26.604529991851916	26.25127869455484	23.640392492097014
155-159	23.381639665697612	26.81085462750649	26.35790787597983	23.449597830816064
160-164	23.157137769094632	27.002300131020444	26.54445525138483	23.296106848500095
165-169	23.07929142693111	27.319239924282567	26.630618188078003	22.970850460708316
170-173	22.915695222417092	27.497585301046136	26.760399339559005	22.826320136977767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.0
5	0.5
6	2.5
7	4.5
8	11.0
9	31.0
10	77.5
11	158.5
12	301.0
13	575.0
14	1064.0
15	1888.5
16	3270.5
17	5232.0
18	7933.5
19	11512.5
20	16291.0
21	22315.0
22	29487.0
23	37762.5
24	47347.5
25	58264.0
26	69767.0
27	82352.5
28	96273.5
29	110800.0
30	125542.0
31	141875.0
32	159587.0
33	177364.5
34	196283.0
35	215413.0
36	235480.5
37	255764.0
38	274759.5
39	292599.0
40	308764.5
41	322023.0
42	331115.0
43	336038.0
44	336554.0
45	333840.0
46	326984.0
47	317340.0
48	305561.0
49	293254.5
50	283212.0
51	273854.5
52	267319.0
53	265060.5
54	265309.0
55	269183.5
56	275586.5
57	282958.0
58	289758.0
59	293798.5
60	294242.0
61	290096.0
62	279670.5
63	262400.5
64	240009.0
65	213809.5
66	184609.5
67	154166.0
68	125490.5
69	99142.0
70	76022.5
71	56952.0
72	41163.0
73	28766.5
74	19536.5
75	12788.5
76	8313.0
77	5373.5
78	3301.0
79	1999.5
80	1216.0
81	703.5
82	384.5
83	219.0
84	129.5
85	65.5
86	24.0
87	14.0
88	8.5
89	3.5
90	1.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1827.0
92-93	4389.0
94-95	7079.0
96-97	9666.0
98-99	12228.0
100-101	14549.0
102-103	17337.0
104-105	20761.0
106-107	22947.0
108-109	25434.0
110-111	27576.0
112-113	29862.0
114-115	31909.0
116-117	34486.0
118-119	36875.0
120-121	39676.0
122-123	41917.0
124-125	44709.0
126-127	47621.0
128-129	53498.0
130-131	108931.0
132-133	133839.0
134-135	161112.0
136-137	163153.0
138-139	170161.0
140-141	166854.0
142-143	168974.0
144-145	172391.0
146-147	186583.0
148-149	171093.0
150-151	174811.0
152-153	177542.0
154-155	182239.0
156-157	189927.0
158-159	197854.0
160-161	198555.0
162-163	211060.0
164-165	213940.0
166-167	222824.0
168-169	227535.0
170-171	268671.0
172-173	6661773.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.62920537366134
#Duplication Level	Percentage of total
1	69.78240085519658
2	19.504156641626444
3	6.675612850160316
4	2.3172380655440494
5	0.8534730229628952
6	0.3898843639619836
7	0.16711637228154874
8	0.08651006077534759
9	0.062071396151112124
>10	0.16153637133964838
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.046361517212331E-6	0.0	0.0	9.046361517212331E-6	0.0	0.0
2	9.046361517212331E-6	9.046361517212331E-6	9.046361517212331E-6	9.046361517212331E-6	0.0	0.0
3	1.8092723034424662E-5	3.6185446068849325E-5	3.6185446068849325E-5	9.046361517212331E-6	0.0	0.0
4	1.8092723034424662E-5	5.4278169103273984E-5	5.4278169103273984E-5	1.8092723034424662E-5	0.0	0.0
5	2.7139084551636992E-5	8.141725365491098E-5	6.332453062048632E-5	3.6185446068849325E-5	0.0	0.0
6	3.6185446068849325E-5	1.0855633820654797E-4	6.332453062048632E-5	3.6185446068849325E-5	0.0	0.0
7	4.523180758606166E-5	1.176026997237603E-4	7.237089213769865E-5	3.6185446068849325E-5	0.0	0.0
8	5.4278169103273984E-5	1.176026997237603E-4	8.141725365491098E-5	3.6185446068849325E-5	0.0	0.0
9	6.332453062048632E-5	1.5378814579260962E-4	9.046361517212332E-5	5.4278169103273984E-5	0.0	0.0
10-14	8.503579826179591E-5	1.8997359186145895E-4	1.0493779359966305E-4	7.779870904802605E-5	0.0	0.0
15-19	9.408215977900825E-5	2.243497656268658E-4	1.3569542275818498E-4	1.2483978893753018E-4	0.0	0.0
20-24	1.0493779359966302E-4	2.967206577645645E-4	1.4293251197195484E-4	1.7730868573736166E-4	0.0	0.0
25-29	1.284583335444151E-4	3.473802822609536E-4	1.9540140877178633E-4	2.243497656268658E-4	1.8092723034424662E-5	0.0
30-34	4.88503521929466E-4	3.76328639116033E-4	2.569166670888302E-4	2.478703055716179E-4	1.8092723034424662E-5	3.6185446068849326E-6
35-39	0.0018110815757459088	4.3060680821930695E-4	2.9310211315767957E-4	3.075762915852193E-4	2.3520539944752058E-5	2.7139084551636992E-5
40-44	0.00318431925405874	4.758386158053685E-4	3.3109683152997136E-4	3.455710099575111E-4	4.161326297917672E-5	5.608744140671645E-5
45-49	0.004045532870497355	5.138333341776604E-4	3.636637329919357E-4	3.7813791141947544E-4	6.694307522737125E-5	6.332453062048632E-5
50-54	0.004642592730633369	5.753485924947043E-4	4.0346772366767E-4	4.1251408518488237E-4	9.227288747556579E-5	6.332453062048632E-5
55-59	0.005071390266549233	6.531473015427303E-4	4.486995312537316E-4	4.740293435019262E-4	1.1941197202720277E-4	7.056161983425618E-5
60-64	0.005547228882354602	6.911420199150222E-4	4.758386158053685E-4	4.975498834466782E-4	1.5559741809605208E-4	8.322652595835344E-5
65-69	0.005976026418270467	7.363738275010837E-4	5.174518787845453E-4	5.572558694602796E-4	1.7369014113047674E-4	9.046361517212332E-5
70-74	0.1014133311525571	8.015076304250127E-4	5.717300478878194E-4	6.115340385635536E-4	2.2615903793030823E-4	1.0855633820654798E-4
75-79	0.18334441814164576	8.376930764938618E-4	6.024876770463413E-4	6.314360339014207E-4	3.093855638886617E-4	1.6102523500637948E-4
80-84	0.18416220922280177	8.612136164386138E-4	6.459102123289605E-4	6.549565738461727E-4	6.368638508117481E-4	1.6283450730982197E-4
85-89	0.18432866227471845	8.684507056523837E-4	7.038069260391193E-4	6.730492968805974E-4	6.603843907565002E-4	1.6283450730982197E-4
90-94	0.18442636297910434	9.028268794177907E-4	7.436109167148536E-4	6.857142030046948E-4	6.603843907565002E-4	1.6283450730982197E-4
95-99	0.1846163365709658	9.661514100382769E-4	7.779870904802605E-4	7.454201890182961E-4	6.603843907565002E-4	1.6283450730982197E-4
100-104	0.1848153565243445	9.987183115002414E-4	8.123632642456673E-4	7.978890858181276E-4	6.603843907565002E-4	1.6464377961326442E-4
105-109	0.18496190758092332	0.001067470659031055	8.919712455971358E-4	8.30455987280092E-4	6.603843907565002E-4	1.7188086882703428E-4
110-114	0.1852423447879569	0.0011163210112240017	9.353937808797549E-4	8.630228887420565E-4	6.603843907565002E-4	1.7188086882703428E-4
115-119	0.18547393164279752	0.0011434600957756387	9.89671949983029E-4	8.937805179005784E-4	6.640029353633851E-4	1.7188086882703428E-4
120-124	0.18672232953217285	0.0011832640864513728	0.0010240481237484358	9.08254696328118E-4	6.694307522737126E-4	1.7188086882703428E-4
125-129	0.18924626439547507	0.001226686621733992	0.0010801355651551524	9.480586870038523E-4	7.001883814322345E-4	1.7188086882703428E-4
130-134	0.19184437942321847	0.0013008667861751332	0.0011036561050999044	9.679606823417194E-4	7.056161983425618E-4	1.7911795804080415E-4
135-139	0.19455286006147182	0.0013406707768508675	0.0011235581004377716	9.86053405376144E-4	7.056161983425618E-4	1.8092723034424663E-4
140-144	0.1964381218016589	0.0014021860351679113	0.0011543157295962937	0.0010421408467828606	7.074254706460043E-4	1.8092723034424663E-4
145-149	0.19777336476159943	0.0014618920211815127	0.001172408452630718	0.0010620428421207276	7.146625598597741E-4	1.8454577495113155E-4
150-154	0.19962967814493138	0.001507123828767574	0.001186882631058258	0.0010837541097620373	7.164718321632166E-4	1.8997359186145895E-4
155-159	0.2018315625382209	0.0015433092748364237	0.0012013568094857974	0.0011054653774033468	7.327552828941988E-4	1.8997359186145895E-4
160-162	0.20376024681369054	0.0015650205424777334	0.00120316608178924	0.0011277797358124706	7.327552828941988E-4	1.9298904570052972E-4
>>END_MODULE
