##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479095
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11210750
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.82501179671298	41.0	40.0	41.0	38.0	41.0
2	39.908610307071335	41.0	40.0	41.0	38.0	41.0
3	39.95976121133733	41.0	40.0	41.0	38.0	41.0
4	39.954455678701244	41.0	40.0	41.0	38.0	41.0
5	39.93265624512187	41.0	40.0	41.0	38.0	41.0
6	39.904030238833265	41.0	40.0	41.0	38.0	41.0
7	39.87650692415762	41.0	40.0	41.0	38.0	41.0
8	39.8558529982383	41.0	40.0	41.0	38.0	41.0
9	39.81556746872422	41.0	40.0	41.0	38.0	41.0
10-14	39.75851242780367	40.8	39.8	41.0	37.8	41.0
15-19	39.72826542381197	41.0	39.8	41.0	37.4	41.0
20-24	39.50660924559016	40.0	39.0	41.0	36.4	41.0
25-29	39.130336703610375	40.0	38.2	41.0	35.2	41.0
30-34	38.593551189706304	40.0	37.0	41.0	34.8	41.0
35-39	37.95158653970519	39.0	35.4	41.0	34.0	41.0
40-44	37.47452034877239	37.8	35.0	40.4	34.0	41.0
45-49	36.82567089623798	36.4	35.0	39.8	33.0	41.0
50-54	36.252478344446175	35.2	35.0	39.0	33.0	41.0
55-59	35.760827437950184	35.0	35.0	37.2	33.0	40.0
60-64	34.97605564302121	34.8	34.0	36.0	32.2	39.0
65-69	35.1975106393417	35.0	35.0	36.0	33.0	39.0
70-74	35.04341270655398	35.0	34.2	35.4	33.0	38.4
75-79	34.97836002051603	35.0	34.0	35.0	33.0	38.6
80-84	34.98292005441206	35.0	34.0	35.0	33.0	39.2
85-89	38.373505715496286	39.6	37.6	39.8	35.0	40.8
90-94	39.46265560824982	40.4	39.0	41.0	36.0	41.0
95-99	39.25756433851235	40.0	38.6	41.0	35.4	41.0
100-104	39.17392411766958	40.0	38.0	41.0	35.0	41.0
105-109	38.95699546460076	40.0	38.0	41.0	35.0	41.0
110-114	38.63192454103262	40.0	37.4	41.0	34.6	41.0
115-119	38.19936281587803	39.6	36.0	41.0	34.0	41.0
120-124	37.46991279697856	38.4	35.0	40.4	33.6	40.6
125-129	37.38401970051664	37.8	35.0	40.4	33.0	41.0
130-134	36.87629008254682	36.6	35.0	40.0	33.0	41.0
135-139	36.2972741253781	35.4	35.0	39.0	33.0	41.0
140-144	35.750232940730506	35.0	35.0	37.4	33.0	40.2
145-149	35.23828928906766	35.0	34.6	36.4	33.0	39.0
150-154	34.81947103456199	35.0	34.0	35.4	33.0	37.2
155-159	34.50646763305097	35.0	34.0	35.0	32.2	36.2
160-164	34.28565308974247	35.0	34.0	35.0	32.0	36.0
165-169	34.12770389852354	35.0	34.0	35.0	32.0	35.4
170-173	33.64115199959706	34.75	33.25	35.0	30.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	8.0
30	262.0
31	3801.0
32	27575.0
33	129638.0
34	440195.0
35	1260301.0
36	2847650.0
37	3346830.0
38	2483463.0
39	659870.0
40	11157.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.83671030038133	25.563954240349663	26.82430702673773	23.775028432531276
2	23.775965033561537	25.84761947238142	26.657440403184445	23.7189750908726
3	23.512102223312446	26.122989095288006	26.595455255000783	23.76945342639877
4	23.850429275472205	26.122230894453985	26.135717949289745	23.89162188078407
5	24.1832170015387	26.247690832459913	25.865869812456793	23.70322235354459
6	23.507615458377003	26.44632161095377	26.193046852351536	23.853016078317683
7	23.80619494681444	26.270775817853398	26.062564948821443	23.860464286510716
8	24.214062395468634	26.1639051802957	25.889998438998283	23.732033985237383
9	23.571438128581942	26.250473875521262	26.099458109403923	24.078629886492877
10-14	23.870030105033116	26.172413085654394	26.067371050108157	23.890185759204336
15-19	23.755143946658343	26.099402805343086	26.184942131436344	23.960511116562227
20-24	23.841755457931004	26.079252503177752	26.063849430234377	24.01514260865687
25-29	23.895559173115092	26.13020359922396	25.961866958053655	24.012370269607295
30-34	23.729919942911938	26.104480074928084	26.059937113930825	24.105662868229157
35-39	23.8354686350155	26.103466761813436	25.982281292509423	24.078783310661642
40-44	23.99466226612849	25.98847891532681	25.844672301139532	24.17218651740517
45-49	24.087291216020336	25.972073233280558	25.797758401534242	24.142877149164864
50-54	24.109025711928282	25.961613629774995	25.791064826171308	24.138295832125415
55-59	24.095345984880584	25.997493477242823	25.743480141828158	24.163680396048438
60-64	23.944328434761278	26.01351738286912	25.865869812456793	24.176284369912807
65-69	23.984238342662177	26.068047186851906	25.816968534665385	24.13074593582053
70-74	23.991825703008274	26.086952255647482	25.77548692103561	24.145735120308633
75-79	23.92450995696095	26.120548580603437	25.76878799366679	24.186153468768815
80-84	23.9911031822135	26.167749704524674	25.689931538924693	24.151215574337133
85-89	23.25393216332538	25.86836206319827	27.052231117454227	23.825474656022124
90-94	23.607768568908952	26.236839353478164	26.182017973532563	23.97337410408032
95-99	23.715225509200877	26.225864521206972	26.03128102138519	24.027628948206964
100-104	23.6071808293635	26.20410678104757	26.15786343815355	24.03084895143538
105-109	23.68678112074747	26.1003261034119	26.13439593696924	24.078496838871395
110-114	23.75443628128127	26.13094064008317	25.980816073107615	24.133807005527945
115-119	23.6813649490793	26.1560755398214	25.997564949473777	24.164994561625523
120-124	23.751024437705205	26.162624734936102	26.013388289415918	24.07296253794278
125-129	23.818647507249064	26.134894475661863	25.972351939582637	24.074106077506432
130-134	23.85755421762204	26.134142329175447	25.950584122746385	24.05771933045613
135-139	23.825847715952467	26.27518461878826	25.985379761453792	23.91358790380548
140-144	23.723828113949008	26.36192474313778	26.072142350072202	23.842104792841003
145-149	23.529281719194252	26.539956477738404	26.217528614259656	23.713233188807685
150-154	23.410879138288948	26.688625424093377	26.3528978193628	23.54759761825488
155-159	23.30622029816102	26.877087332021034	26.446945966403284	23.369746403414666
160-164	23.082055622687257	27.066914190122365	26.634851886478778	23.216178300711597
165-169	23.00671628670862	27.37484152489902	26.704411313456927	22.914030874935438
170-173	22.829133092458704	27.575570448613636	26.832277847996426	22.76301861093123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	1.5
7	4.5
8	18.5
9	34.0
10	64.5
11	157.5
12	320.5
13	600.0
14	1156.0
15	2054.0
16	3420.5
17	5598.0
18	8414.0
19	12182.0
20	17298.0
21	23487.0
22	30925.0
23	39973.5
24	49954.5
25	60850.0
26	72880.5
27	86129.5
28	100502.5
29	115345.0
30	130982.0
31	147845.0
32	165323.5
33	183639.5
34	202892.5
35	222168.0
36	242138.5
37	262891.0
38	282870.0
39	300797.0
40	316401.0
41	329253.0
42	337978.5
43	343209.0
44	344165.5
45	340193.0
46	332613.0
47	321830.0
48	309722.5
49	296698.5
50	285047.5
51	276432.5
52	269472.0
53	265977.0
54	266639.5
55	270022.5
56	275459.5
57	282476.0
58	288901.5
59	292500.0
60	292891.5
61	288315.5
62	278124.5
63	261045.5
64	238661.0
65	213064.5
66	184082.5
67	154299.0
68	125498.0
69	99167.0
70	76337.5
71	57124.5
72	41474.5
73	29075.0
74	19756.0
75	13199.5
76	8573.0
77	5397.0
78	3330.5
79	2093.0
80	1283.0
81	704.0
82	407.0
83	233.5
84	122.5
85	60.5
86	37.0
87	20.5
88	6.5
89	3.5
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1976.0
92-93	4895.0
94-95	7719.0
96-97	10652.0
98-99	13436.0
100-101	16058.0
102-103	18717.0
104-105	22330.0
106-107	24438.0
108-109	26819.0
110-111	29319.0
112-113	31269.0
114-115	33755.0
116-117	36092.0
118-119	38229.0
120-121	40905.0
122-123	43409.0
124-125	46150.0
126-127	49267.0
128-129	53509.0
130-131	115063.0
132-133	139675.0
134-135	168776.0
136-137	174264.0
138-139	175453.0
140-141	176039.0
142-143	172031.0
144-145	174719.0
146-147	186911.0
148-149	176505.0
150-151	177047.0
152-153	182655.0
154-155	184222.0
156-157	194513.0
158-159	194412.0
160-161	203060.0
162-163	211472.0
164-165	217397.0
166-167	221232.0
168-169	229834.0
170-171	258602.0
172-173	6727924.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.66376527149107
#Duplication Level	Percentage of total
1	69.80397919834664
2	19.662993364036414
3	6.467603906689086
4	2.3118208130901774
5	0.8482064302707636
6	0.3826313725222751
7	0.18960611526517718
8	0.10294588636394389
9	0.06514408876325338
>10	0.16506882465226624
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.676002943603238E-5	1.7840019624021587E-5	0.0	0.0	0.0
2	0.0	3.5680039248043174E-5	3.5680039248043174E-5	8.920009812010793E-6	0.0	0.0
3	0.0	7.136007849608635E-5	7.136007849608635E-5	1.7840019624021587E-5	0.0	0.0
4	0.0	8.028008830809713E-5	8.028008830809713E-5	4.4600049060053966E-5	0.0	0.0
5	0.0	8.028008830809713E-5	8.920009812010793E-5	4.4600049060053966E-5	0.0	0.0
6	8.920009812010793E-6	8.028008830809713E-5	9.812010793211873E-5	4.4600049060053966E-5	0.0	0.0
7	1.7840019624021587E-5	1.0704011774412952E-4	1.0704011774412952E-4	5.352005887206476E-5	0.0	0.0
8	2.676002943603238E-5	1.0704011774412952E-4	1.1596012755614031E-4	5.352005887206476E-5	0.0	0.0
9	2.676002943603238E-5	1.427201569921727E-4	1.1596012755614031E-4	6.244006868407555E-5	0.0	0.0
10-14	3.92480431728475E-5	1.7661619427781372E-4	1.3915215306736838E-4	7.671208438329281E-5	0.0	0.0
15-19	6.600807260887987E-5	2.3370425707468275E-4	1.5877617465379212E-4	8.384809223290145E-5	1.427201569921727E-5	0.0
20-24	8.741609615770579E-5	2.7295230024753026E-4	2.158642374506612E-4	1.1774412951854249E-4	3.389603728564101E-5	0.0
25-29	9.812010793211873E-5	3.336083669692036E-4	2.800883080971389E-4	1.5342416876658563E-4	3.5680039248043174E-5	0.0
30-34	4.83464531810985E-4	3.81776419954062E-4	3.1933635126998633E-4	1.980242178266396E-4	4.995205494726044E-5	0.0
35-39	0.0018500100350110384	4.38864482750931E-4	3.47880382668421E-4	2.551122806235087E-4	6.600807260887987E-5	3.5680039248043173E-6
40-44	0.0031077314185045607	4.94168543585398E-4	4.0496844546529E-4	2.854403139843454E-4	9.09841000825101E-5	3.746404121044533E-5
45-49	0.003997948397743237	6.047766652543318E-4	4.816805298485828E-4	3.3360836696920366E-4	9.812010793211873E-5	7.849608634569499E-5
50-54	0.004697277167004884	6.672167339384073E-4	5.17360569096626E-4	3.978324376156814E-4	1.1952813148094463E-4	1.0525611578172736E-4
55-59	0.005378765916642508	7.20736792810472E-4	5.673126240438865E-4	4.192404611645073E-4	1.5164016680418345E-4	1.2309613540574895E-4
60-64	0.005885422473964721	7.582008340209174E-4	6.047766652543318E-4	4.531364984501483E-4	1.6948018642820508E-4	1.248801373681511E-4
65-69	0.0065223111745422915	8.063688870057756E-4	6.690007359008096E-4	4.8346453181098497E-4	1.6948018642820508E-4	1.2844814129295545E-4
70-74	0.1045407309948041	8.170728987801888E-4	7.08248779073657E-4	5.066565573222131E-4	1.8732020605222665E-4	1.5877617465379215E-4
75-79	0.18962335258568785	8.33128916441808E-4	7.706888477577326E-4	5.298485828334411E-4	2.765203041723346E-4	1.7840019624021586E-4
80-84	0.19048502553352806	8.705929576522534E-4	8.135048948553844E-4	5.583926142318757E-4	5.548246103070714E-4	1.7840019624021586E-4
85-89	0.19067412974154271	9.009209910130902E-4	8.563209419530362E-4	5.815846397431038E-4	5.780166358182995E-4	1.7840019624021586E-4
90-94	0.19083290591619648	9.544410498851548E-4	8.973529870882858E-4	6.047766652543318E-4	5.869366456303102E-4	1.7840019624021586E-4
95-99	0.19101487411636153	9.77633075396383E-4	9.366010302611332E-4	6.368887005775705E-4	5.887206475927124E-4	1.7840019624021586E-4
100-104	0.19121111433222576	0.0010168811185692305	9.490890439979485E-4	6.600807260887987E-4	6.029926632919296E-4	1.7840019624021586E-4
105-109	0.19142697856967641	0.0010828891911781103	9.829850812835895E-4	6.85056753562429E-4	6.226166848783533E-4	1.7840019624021586E-4
110-114	0.1916303547933903	0.001156033271636599	0.0010258011283812412	7.100327810360592E-4	6.333206966527663E-4	1.7840019624021586E-4
115-119	0.19189438708382578	0.001236313359944696	0.0010971612068773275	7.439288183217002E-4	6.511607162767879E-4	1.8375220212742233E-4
120-124	0.19315567647124413	0.0013076734384407822	0.0011399772539749792	7.920968713065584E-4	6.582967241263967E-4	1.9267221193943314E-4
125-129	0.19557835113618624	0.0013611934973128473	0.0011649532814486095	8.581049439154384E-4	6.725687398256139E-4	2.0516022567624825E-4
130-134	0.1980777378855117	0.0014129295542225097	0.001181009299110229	8.88432977276275E-4	6.779207457128203E-4	2.0516022567624825E-4
135-139	0.20063599669959636	0.001471801618981781	0.0012309613540574895	9.09841000825101E-4	6.814887496376246E-4	2.0516022567624825E-4
140-144	0.20254844680329148	0.0015128336641170308	0.0012666413933055327	9.33033026336329E-4	6.97544767299244E-4	2.0516022567624825E-4
145-149	0.2038971522868675	0.0015574337131770844	0.0012880494168543586	9.508730459603506E-4	7.046807751488526E-4	2.0516022567624825E-4
150-154	0.20558660214526236	0.0015895457485003233	0.0013112414423655865	9.77633075396383E-4	7.118167829984612E-4	2.0516022567624825E-4
155-159	0.20756327631960395	0.0016145217759739536	0.0013201614521775975	0.0010061771067948176	7.225207947728743E-4	2.0516022567624825E-4
160-162	0.20952508381092552	0.0016442551420139896	0.0013201614521775975	0.0010258011283812412	7.225207947728743E-4	2.0516022567624825E-4
>>END_MODULE
