##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479096
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10971142
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.900352214928944	41.0	40.0	41.0	38.0	41.0
2	39.96726421005215	41.0	40.0	41.0	38.0	41.0
3	40.00459487262128	41.0	40.0	41.0	38.0	41.0
4	39.98445868260569	41.0	40.0	41.0	38.0	41.0
5	39.9626902103719	41.0	40.0	41.0	38.0	41.0
6	39.92507243092834	41.0	40.0	41.0	38.0	41.0
7	39.88620245731939	41.0	40.0	41.0	38.0	41.0
8	39.85798297023227	41.0	40.0	41.0	38.0	41.0
9	39.808415386474806	41.0	40.0	41.0	38.0	41.0
10-14	39.753504803784324	40.4	39.8	41.0	37.8	41.0
15-19	39.785629244430524	41.0	40.0	41.0	37.6	41.0
20-24	39.55139530597635	40.2	39.0	41.0	36.6	41.0
25-29	39.15454998212583	40.0	38.2	41.0	35.2	41.0
30-34	38.5962214325546	40.0	37.0	41.0	34.8	41.0
35-39	37.92511570810039	39.0	35.4	41.0	34.0	41.0
40-44	37.416162583621656	37.8	35.0	40.2	33.8	41.0
45-49	36.74351816793548	36.4	35.0	39.8	33.0	41.0
50-54	36.14904530449063	35.2	35.0	38.8	33.0	40.8
55-59	35.65061292616575	35.0	34.8	37.2	33.0	40.0
60-64	34.824511577737304	34.8	33.4	36.0	31.6	39.0
65-69	35.12440701250608	35.0	34.0	36.0	33.0	39.0
70-74	34.99318457458667	35.0	34.0	35.8	32.4	39.0
75-79	34.96561635971898	35.0	34.0	35.0	32.0	39.8
80-84	35.02924351904295	35.0	34.0	35.6	32.0	40.0
85-89	38.423134382911094	39.6	37.4	40.4	35.0	41.0
90-94	39.4757796001238	40.4	39.0	41.0	36.0	41.0
95-99	39.239678172643735	40.0	38.4	41.0	35.4	41.0
100-104	39.186507045488305	40.0	38.2	41.0	35.0	41.0
105-109	38.96542153392942	40.0	38.0	41.0	35.0	41.0
110-114	38.63702256946536	40.0	37.4	41.0	34.6	41.0
115-119	38.208585791924165	39.8	36.0	41.0	34.0	41.0
120-124	37.481653835989974	38.4	35.0	40.4	33.4	40.6
125-129	37.42553528049243	37.8	35.0	40.8	33.6	41.0
130-134	36.923450228582205	36.6	35.0	40.0	33.0	41.0
135-139	36.3606102830967	35.4	35.0	39.2	33.0	41.0
140-144	35.82103292102782	35.0	35.0	37.6	33.0	40.4
145-149	35.30826358679724	35.0	35.0	36.4	33.0	39.0
150-154	34.8998253840971	35.0	34.0	35.6	33.0	37.4
155-159	34.57355743475	35.0	34.0	35.0	33.0	36.4
160-164	34.34503269087355	35.0	34.0	35.0	32.0	36.0
165-169	34.18808145769889	35.0	34.0	35.0	32.0	35.6
170-173	33.587152759231984	34.5	33.25	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	211.0
31	3064.0
32	22558.0
33	110287.0
34	394463.0
35	1211380.0
36	2827118.0
37	3339869.0
38	2439001.0
39	613970.0
40	9215.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.963284770172514	25.4743854377238	26.65943982859761	23.902889963506077
2	23.852421197355753	25.78123590051063	26.558885118796198	23.807457783337412
3	23.52531760139464	26.081897399559683	26.533846704381368	23.858938294664313
4	23.940260731289413	26.009944999344643	26.126642057864167	23.923152211501776
5	24.239555007126878	26.13916582248229	25.80572742564083	23.81555174475
6	23.601891216064836	26.36667176489011	26.099179100954125	23.932257918090933
7	23.86087975162476	26.16630976064297	26.016307144689222	23.95650334304305
8	24.296085129515234	26.115102693958388	25.814778443301527	23.774033733224854
9	23.631979241541128	26.185669641319016	26.030899973767546	24.151451143372313
10-14	23.937737748722967	26.100770548772406	26.012134379447467	23.949357323057164
15-19	23.814838965715694	26.015692805726147	26.132439084281287	24.037029144276868
20-24	23.894444169987043	26.018819189469976	26.00016297300682	24.08657366753616
25-29	23.943439980997418	26.054091725364596	25.91265704153679	24.089811252101196
30-34	23.772775887870196	26.040862473569298	26.012916431124488	24.173445207436018
35-39	23.872854803993967	26.049517907980775	25.92876839986211	24.148858888163147
40-44	24.039544834986184	25.922187498803677	25.78589175128715	24.252375914922986
45-49	24.140491482108242	25.902758345484912	25.736474835527606	24.22027533687924
50-54	24.16479888784595	25.894943297607487	25.737559499275463	24.2026983152711
55-59	24.140422209465523	25.926385785545385	25.703145579557717	24.23004642543137
60-64	23.959050024145164	25.945311800722294	25.82978508527189	24.265853089860652
65-69	24.009595354795334	25.99990228911448	25.786748544499744	24.203753811590442
70-74	24.004996015911562	26.01289090962454	25.75163642946195	24.230476645001954
75-79	23.92261443703855	26.047025915807126	25.77828452133789	24.25207512581644
80-84	23.959409148108737	26.099399679632256	25.71214737718279	24.229043795076212
85-89	23.34169405518587	25.76884156635654	26.96164902432217	23.927815354135422
90-94	23.700013429278982	26.15924353318222	26.095266192834053	24.045476844704748
95-99	23.80389745275417	26.15215359716582	25.944646766237295	24.099302183842713
100-104	23.710787074816846	26.128491655928045	26.057863100618857	24.102858168636253
105-109	23.77411832718244	26.031126452312296	26.03643298862505	24.158322231880213
110-114	23.837006807595593	26.05484627265065	25.91152476569742	24.19662215405634
115-119	23.77835446824169	26.07919978365829	25.926937467020565	24.215508281079448
120-124	23.82379081915035	26.093929905780634	25.943516346741358	24.138762928327658
125-129	23.902873873104213	26.076778996749056	25.895130195024784	24.125216935121944
130-134	23.927271037316515	26.08366488854164	25.895531573694118	24.093532500447722
135-139	23.87685977605156	26.213546677245347	25.95878853212402	23.950805014579068
140-144	23.748921613819896	26.319703489057595	26.056634789115535	23.874740108006982
145-149	23.544824231551782	26.51634937933116	26.195101678596604	23.743724710520446
150-154	23.423279686006243	26.658640055820236	26.35072518350946	23.56735507466406
155-159	23.305550400327053	26.881824375595905	26.470298888662047	23.342326335415002
160-164	23.0565774852038	27.113240108789753	26.66826833744324	23.16191406856321
165-169	22.968528850045068	27.430869498269683	26.770459903022253	22.830141748662996
170-173	22.812070365973632	27.65786603378466	26.87638222386164	22.65368137638006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.0
6	1.5
7	7.5
8	17.5
9	34.5
10	69.0
11	147.5
12	305.5
13	563.5
14	1042.5
15	1919.0
16	3188.5
17	5027.0
18	7797.5
19	11585.0
20	16320.5
21	22185.5
22	29295.5
23	37844.5
24	47446.0
25	58059.5
26	69786.0
27	82690.0
28	96316.5
29	110242.5
30	125361.5
31	141641.5
32	159018.5
33	177068.5
34	195788.0
35	214995.0
36	234812.0
37	254651.0
38	274088.5
39	292183.5
40	307412.5
41	320541.0
42	329797.0
43	335165.5
44	335156.0
45	331225.0
46	324717.0
47	314763.5
48	303368.5
49	291794.5
50	280983.5
51	271657.0
52	265359.0
53	262536.0
54	263494.5
55	266998.0
56	272689.0
57	280299.5
58	286620.5
59	290386.0
60	290672.0
61	285493.0
62	275252.5
63	258569.5
64	235774.0
65	209772.0
66	181476.5
67	152345.0
68	123821.5
69	97749.0
70	75270.5
71	56166.5
72	40648.5
73	28614.5
74	19560.0
75	12975.0
76	8476.0
77	5369.0
78	3296.5
79	1981.5
80	1206.5
81	736.0
82	427.0
83	241.5
84	137.0
85	65.5
86	33.5
87	16.0
88	4.0
89	3.5
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1873.0
92-93	4641.0
94-95	7296.0
96-97	9989.0
98-99	12738.0
100-101	15355.0
102-103	18275.0
104-105	22469.0
106-107	24728.0
108-109	27407.0
110-111	29614.0
112-113	32094.0
114-115	35064.0
116-117	37711.0
118-119	40348.0
120-121	43546.0
122-123	46452.0
124-125	49514.0
126-127	52362.0
128-129	59384.0
130-131	108967.0
132-133	136166.0
134-135	164829.0
136-137	167892.0
138-139	174900.0
140-141	173691.0
142-143	174804.0
144-145	184177.0
146-147	201024.0
148-149	179882.0
150-151	179897.0
152-153	185023.0
154-155	191750.0
156-157	198743.0
158-159	205575.0
160-161	213142.0
162-163	223374.0
164-165	229428.0
166-167	238673.0
168-169	249525.0
170-171	331234.0
172-173	6287586.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.85387654341785
#Duplication Level	Percentage of total
1	70.0956693150633
2	19.483322767799844
3	6.570526765282468
4	2.1850242238533304
5	0.8251602835410257
6	0.345044985286798
7	0.18048676897136615
8	0.09580458291737338
9	0.06312490755649765
>10	0.15583539972797983
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	9.114821410569657E-6	0.0	0.0	0.0	0.0
2	9.114821410569657E-6	1.8229642821139313E-5	2.734446423170897E-5	9.114821410569657E-6	0.0	0.0
3	9.114821410569657E-6	1.8229642821139313E-5	2.734446423170897E-5	9.114821410569657E-6	0.0	0.0
4	9.114821410569657E-6	1.8229642821139313E-5	3.645928564227863E-5	2.734446423170897E-5	0.0	0.0
5	1.8229642821139313E-5	2.734446423170897E-5	3.645928564227863E-5	3.645928564227863E-5	0.0	0.0
6	1.8229642821139313E-5	2.734446423170897E-5	4.557410705284828E-5	3.645928564227863E-5	0.0	0.0
7	1.8229642821139313E-5	3.645928564227863E-5	4.557410705284828E-5	4.557410705284828E-5	0.0	0.0
8	1.8229642821139313E-5	4.557410705284828E-5	7.291857128455725E-5	6.38037498739876E-5	0.0	0.0
9	4.557410705284828E-5	7.291857128455725E-5	8.203339269512691E-5	6.38037498739876E-5	9.114821410569657E-6	0.0
10-14	5.6511892745531866E-5	1.0390896408049408E-4	1.093778569268359E-4	8.932524982358263E-5	9.114821410569657E-6	0.0
15-19	6.38037498739876E-5	1.6042085682602597E-4	1.3672232115854484E-4	1.2578453546586125E-4	1.640667853902538E-5	0.0
20-24	7.474153556667119E-5	2.0052607103253247E-4	1.7135864251870955E-4	1.4401417828700057E-4	1.8229642821139313E-5	0.0
25-29	8.932524982358263E-5	2.588609280601782E-4	2.1146385672521606E-4	1.4766010685122842E-4	3.0990392795936835E-5	0.0
30-34	4.903773918886475E-4	3.226646779341658E-4	2.6615278518863397E-4	1.7318160680082346E-4	3.645928564227863E-5	0.0
35-39	0.0019122895319375137	3.591239635764445E-4	3.1901874936993797E-4	1.8958828533984884E-4	6.927264272032939E-5	1.2760749974797519E-5
40-44	0.0031482593152107594	3.9376028493660914E-4	3.7735360639758376E-4	2.1328682100732994E-4	9.661710695203836E-5	3.281335707805076E-5
45-49	0.004056095527703497	4.393343919894575E-4	4.211047491683181E-4	2.406312852390389E-4	1.1120082120894982E-4	5.2865964181304014E-5
50-54	0.004695955990725488	4.6120996337482465E-4	4.5209514196425493E-4	2.533920352138365E-4	1.458371425691145E-4	7.291857128455725E-5
55-59	0.005241022311077552	4.976692490171032E-4	5.013151775813309E-4	2.880283565740012E-4	1.6588974967236776E-4	7.474153556667119E-5
60-64	0.005868122024124745	5.323055703772679E-4	5.231907489666983E-4	3.0808096367725433E-4	1.9688014246830457E-4	9.661710695203836E-5
65-69	0.006327509023217456	5.83348570276458E-4	5.632959631732047E-4	3.17195785087824E-4	2.1875571385367173E-4	1.1849267833740552E-4
70-74	0.1077061986801374	6.270997130471925E-4	6.052241416618252E-4	3.4089432075530514E-4	2.533920352138364E-4	1.276074997479752E-4
75-79	0.19431340875908817	6.398604630219898E-4	6.380374987398759E-4	3.7370767783335594E-4	3.536550707301027E-4	1.458371425691145E-4
80-84	0.19516655604311747	6.544441772789013E-4	6.690278915358128E-4	3.9376028493660914E-4	6.799656772284964E-4	1.458371425691145E-4
85-89	0.19531603911425083	6.89080498639066E-4	7.073101414602054E-4	4.083439991935206E-4	7.16424962870775E-4	1.458371425691145E-4
90-94	0.19551291925671913	7.4194646282037E-4	7.419464628203702E-4	4.265736420146599E-4	7.200708914350028E-4	1.458371425691145E-4
95-99	0.19567516307782729	7.802287127447627E-4	7.674679627699651E-4	4.4844921340002705E-4	7.200708914350028E-4	1.458371425691145E-4
100-104	0.19581370836326792	8.039272484122436E-4	8.075731769764715E-4	4.757936776317361E-4	7.200708914350028E-4	1.458371425691145E-4
105-109	0.19593037807732322	8.622621054398894E-4	8.476783911829782E-4	5.104299989919008E-4	7.218938557171168E-4	1.458371425691145E-4
110-114	0.1960743922556102	9.041902839285099E-4	8.713769268504591E-4	5.468892846341793E-4	7.291857128455725E-4	1.458371425691145E-4
115-119	0.19636606654074845	9.80754783777295E-4	9.205969624675352E-4	5.669418917374327E-4	7.346546056919143E-4	1.458371425691145E-4
120-124	0.19780803128790056	0.0010245059265480295	9.771088552130672E-4	5.906404274049137E-4	7.528842485130537E-4	1.458371425691145E-4
125-129	0.20023075081883	0.0010737259621651056	0.0010245059265480293	6.307456416114203E-4	7.656449984878511E-4	1.458371425691145E-4
130-134	0.20330973749132042	0.001113831176371612	0.0010573192836260802	6.562671415610153E-4	7.656449984878511E-4	1.458371425691145E-4
135-139	0.2063686715567076	0.0011794578905277137	0.0010664341050366497	6.92726427203294E-4	7.656449984878511E-4	1.458371425691145E-4
140-144	0.20844502787403535	0.0012268549618626758	0.0010901326407041308	7.18247927152889E-4	7.747598198984208E-4	1.458371425691145E-4
145-149	0.2098086051570566	0.00127060610463341	0.0011193000692179538	7.437694271024839E-4	7.966353912837881E-4	1.458371425691145E-4
150-154	0.21151307676083314	0.0013161802116862583	0.0011357067477569792	7.474153556667118E-4	8.112191055406994E-4	1.458371425691145E-4
155-159	0.21379542804203971	0.0013417017116358532	0.0011539363905781184	7.583531413593953E-4	8.112191055406994E-4	1.458371425691145E-4
160-162	0.21608810945417836	0.001348993568764309	0.0011575823191423463	7.899511889160369E-4	8.112191055406993E-4	1.458371425691145E-4
>>END_MODULE
