##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479118
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8447600
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3296452246792	39.0	39.0	39.0	37.0	39.0
2	40.06008700696056	41.0	40.0	41.0	38.0	41.0
3	40.08444031440882	41.0	40.0	41.0	38.0	41.0
4	40.09697878687438	41.0	40.0	41.0	38.0	41.0
5	40.101393531890714	41.0	40.0	41.0	38.0	41.0
6	40.09334284293764	41.0	40.0	41.0	38.0	41.0
7	40.11338060514229	41.0	40.0	41.0	38.0	41.0
8	40.098308868791136	41.0	40.0	41.0	38.0	41.0
9	40.08504308916142	41.0	40.0	41.0	38.0	41.0
10-14	40.022550073393624	41.0	40.0	41.0	38.0	41.0
15-19	39.909719612671054	41.0	40.0	41.0	38.0	41.0
20-24	39.745357142857145	41.0	39.8	41.0	37.4	41.0
25-29	39.54050146787253	40.2	39.0	41.0	36.8	41.0
30-34	39.46794535726123	40.2	39.0	41.0	36.0	41.0
35-39	39.33509633505374	40.4	38.8	41.0	35.2	41.0
40-44	38.89990174724181	40.0	37.4	41.0	35.0	41.0
45-49	38.32452852881291	39.4	36.0	41.0	35.0	41.0
50-54	37.66055665514465	38.2	35.0	40.6	34.2	41.0
55-59	36.93928538283062	36.6	35.0	39.4	34.0	41.0
60-64	36.13342144514418	35.6	34.8	38.4	33.8	40.2
65-69	35.64700920971637	35.0	35.0	36.8	33.6	39.0
70-74	35.31306214782897	35.0	35.0	36.0	33.2	38.0
75-79	35.06319446943511	35.0	35.0	35.4	33.0	37.0
80-84	34.95573301292675	35.0	35.0	35.0	33.0	36.4
85-89	34.77611297883423	35.0	34.6	35.0	32.6	36.2
90-94	39.57816692921724	40.6	39.8	40.6	37.8	40.6
95-99	39.854044562128614	41.0	40.0	41.0	37.8	41.0
100-104	39.75838563520231	41.0	40.0	41.0	37.0	41.0
105-109	39.62521324440318	41.0	39.4	41.0	36.8	41.0
110-114	39.45375405495941	40.6	39.0	41.0	36.0	41.0
115-119	39.2230037285329	40.0	38.4	41.0	35.0	41.0
120-124	38.9585997684516	40.0	38.0	41.0	35.0	41.0
125-129	38.51737438499106	39.6	37.4	40.6	34.6	41.0
130-134	38.32961755363974	39.8	36.4	41.0	34.0	41.0
135-139	37.99948671204423	39.0	35.2	41.0	34.0	41.0
140-144	37.51965303650314	37.8	35.0	40.6	34.0	41.0
145-149	36.80871838839933	36.4	35.0	39.2	34.0	41.0
150-154	36.10645958333852	35.4	35.0	37.8	34.0	40.2
155-159	35.50382083070137	35.0	35.0	36.4	33.8	38.6
160-164	35.05547157971944	35.0	35.0	35.8	33.0	37.0
165-169	34.762629858302525	35.0	35.0	35.0	33.0	36.0
170-174	34.603211608785045	35.0	35.0	35.0	33.0	36.0
175-178	34.310956488280546	35.0	34.5	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	72.0
30	672.0
31	4412.0
32	21284.0
33	77131.0
34	210695.0
35	487476.0
36	1143385.0
37	2351689.0
38	3065183.0
39	1069435.0
40	16164.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.06756948719163	28.448719162839147	27.422309294947677	22.061402055021546
2	21.77193522420569	28.94123774799943	28.598678914721344	20.688148113073535
3	20.76412235427814	27.595766845021068	29.375171646384775	22.264939154316018
4	21.03592736398504	26.978005587385766	30.0039419480089	21.982125100620294
5	21.750497182631754	27.444860078602208	28.88049860315356	21.92414413561248
6	22.411465978502772	27.241985889483406	28.04307732373692	22.303470808276906
7	22.151569676594534	27.83690042142147	27.943084426345944	22.06844547563805
8	22.133351484445285	27.913383682939536	27.956307116814244	21.99695771580094
9	22.693190965481318	27.63420379752829	27.27695440125006	22.39565083574033
10-14	22.39171125526777	28.02614233628486	27.440494341588145	22.141652066859226
15-19	22.358660447937876	27.96027274018656	27.548449263696195	22.132617548179365
20-24	22.19686538188361	27.952500118376815	27.72781855201477	22.122815947724796
25-29	22.169731047871586	28.06805246460533	27.60972583929163	22.152490648231453
30-34	22.27848856479947	28.07888631090487	27.50071499597519	22.14191012832047
35-39	22.21996543396941	28.06465268241867	27.587527818552015	22.1278540650599
40-44	22.153096737534923	28.16186135707183	27.62314740281263	22.061894502580614
45-49	22.34842795586912	28.03874709976798	27.46193001562574	22.150894928737156
50-54	22.293631327240874	28.045049481509544	27.55047824234102	22.110840948908567
55-59	22.213956626734223	28.087629622614706	27.59870732515744	22.099706425493633
60-64	22.28023107154695	28.066650883091054	27.525971873668258	22.127146171693735
65-69	22.22030399166627	28.064650314882332	27.59424688668971	22.120798806761684
70-74	22.131329608409487	28.213764856290545	27.59335669302524	22.06154884227473
75-79	22.196600217813344	28.167792035607746	27.52051706993702	22.115090676641884
80-84	22.185657464842084	28.209723471755293	27.519316729011788	22.085302334390832
85-89	22.2200104171599	28.250430891614187	27.45397982859037	22.075578862635542
90-94	21.322693559773885	27.71273331246695	28.970375934529745	21.99419719322942
95-99	22.039728034618722	27.651878983791043	27.99877442507564	22.309618556514593
100-104	22.13917992905569	27.794054849145645	27.733381855899847	22.333383365898822
105-109	21.951269548190535	27.790250449260114	28.006314642460968	22.252165360088387
110-114	22.002165241836067	27.64173910776672	28.004333921245994	22.351761729151214
115-119	21.96557467062188	27.70919956614202	27.956932205808627	22.368293557427478
120-124	21.891832799984098	27.79748703981333	27.99415962014093	22.316520540061642
125-129	21.927880160446428	27.75321438978613	27.982833954707832	22.336071495059613
130-134	21.986709460102666	27.749817665094394	27.944304178511796	22.31916869629114
135-139	21.99201268667678	27.808495144885814	27.946184731159384	22.253307437278018
140-144	22.04728113857059	27.79031647709736	27.938803941090523	22.223598443241528
145-149	21.965289404874945	27.806042262710655	28.017659103404675	22.211009229009726
150-154	21.851760259686642	27.931517087271352	28.119311143297697	22.09741150974431
155-159	21.894425765495022	27.950213773126976	28.052015433243987	22.10334502813401
160-164	21.791823212379693	28.085235625970835	28.109531071338257	22.013410090311215
165-169	21.665988500184742	28.20554846109748	28.190036410286346	21.938426628431433
170-174	21.736383677981642	28.303263494321563	28.102641184877942	21.85771164281885
175-178	21.692708498420497	28.35718293639486	28.1369602026612	21.81314836252345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	1.0
7	1.5
8	1.5
9	5.0
10	11.0
11	25.5
12	60.0
13	126.0
14	236.0
15	461.5
16	865.0
17	1518.0
18	2598.0
19	4292.5
20	6885.5
21	10540.5
22	15388.5
23	21662.5
24	29669.0
25	39585.0
26	51456.0
27	65163.5
28	80255.0
29	96947.5
30	115549.0
31	136201.5
32	157733.5
33	179775.0
34	202915.5
35	227251.0
36	250912.5
37	274570.0
38	297830.5
39	317873.0
40	334832.5
41	349169.0
42	359061.5
43	363850.5
44	363573.0
45	356718.5
46	344744.0
47	329376.5
48	310336.0
49	287957.5
50	264294.5
51	240296.5
52	217124.0
53	195845.5
54	175457.0
55	157889.5
56	143440.0
57	130212.5
58	119513.0
59	110145.5
60	101093.5
61	92803.0
62	84635.5
63	75933.5
64	67077.5
65	58447.5
66	50133.5
67	41953.0
68	34087.0
69	27160.5
70	21114.0
71	15885.0
72	11585.0
73	8283.5
74	5797.0
75	3855.5
76	2487.5
77	1552.5
78	963.0
79	579.0
80	319.5
81	185.0
82	98.5
83	55.0
84	26.0
85	9.0
86	3.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1203.0
92-93	2343.0
94-95	3489.0
96-97	4539.0
98-99	5504.0
100-101	6447.0
102-103	7364.0
104-105	8292.0
106-107	9200.0
108-109	10103.0
110-111	11069.0
112-113	11396.0
114-115	13504.0
116-117	12600.0
118-119	13933.0
120-121	15377.0
122-123	14722.0
124-125	15799.0
126-127	18706.0
128-129	17087.0
130-131	19808.0
132-133	19286.0
134-135	90736.0
136-137	78353.0
138-139	73200.0
140-141	69386.0
142-143	68117.0
144-145	73537.0
146-147	73907.0
148-149	75658.0
150-151	75628.0
152-153	82257.0
154-155	72976.0
156-157	71605.0
158-159	72821.0
160-161	74844.0
162-163	77980.0
164-165	82245.0
166-167	85037.0
168-169	95928.0
170-171	90322.0
172-173	91147.0
174-175	103570.0
176-177	238702.0
178-179	6287873.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.11810662963886
#Duplication Level	Percentage of total
1	90.8666162675473
2	7.591718505241414
3	1.0769698126694305
4	0.2370175518641221
5	0.10642004745355221
6	0.04837804861463227
7	0.02381406652871626
8	0.010414917235450736
9	0.008380902065729525
>10	0.030269880779614965
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.73507268336569E-5	2.367536341682845E-5	0.0	1.1837681708414225E-5	1.1837681708414225E-5	0.0
2	6.86585539088025E-4	2.367536341682845E-5	1.1837681708414225E-5	2.367536341682845E-5	2.367536341682845E-5	0.0
3	6.984232207964392E-4	3.551304512524267E-5	1.1837681708414225E-5	2.367536341682845E-5	2.367536341682845E-5	0.0
4	6.984232207964392E-4	4.73507268336569E-5	2.367536341682845E-5	2.367536341682845E-5	2.367536341682845E-5	0.0
5	6.984232207964392E-4	4.73507268336569E-5	3.551304512524267E-5	2.367536341682845E-5	3.551304512524267E-5	0.0
6	7.102609025048534E-4	8.286377195889956E-5	3.551304512524267E-5	3.551304512524267E-5	4.73507268336569E-5	0.0
7	7.220985842132677E-4	8.286377195889956E-5	4.73507268336569E-5	4.73507268336569E-5	4.73507268336569E-5	0.0
8	7.33936265921682E-4	1.0653913537572802E-4	5.918840854207112E-5	4.73507268336569E-5	4.73507268336569E-5	0.0
9	7.33936265921682E-4	1.1837681708414224E-4	8.286377195889956E-5	4.73507268336569E-5	4.73507268336569E-5	0.0
10-14	7.457739476300962E-4	1.4915478952601922E-4	1.325820351342393E-4	6.155594488375397E-5	5.918840854207112E-5	0.0
15-19	7.812869927553388E-4	2.1307827075145607E-4	2.107107344097732E-4	8.996638098394811E-5	5.918840854207112E-5	0.0
20-24	8.073298925138502E-4	2.343860978266016E-4	2.841043610019414E-4	1.2311188976750793E-4	7.339362659216819E-5	0.0
25-29	8.2390264690563E-4	2.7936928831857573E-4	3.290875514939154E-4	1.6336000757611628E-4	1.1837681708414224E-4	0.0
30-34	0.0011032719352242057	3.4092523320232963E-4	4.001136417444008E-4	1.7756522562621335E-4	1.8230029830957906E-4	0.0
35-39	0.002234954306548606	3.859084236943037E-4	4.237890051612292E-4	2.0834319806809038E-4	2.5569392490174724E-4	0.0
40-44	0.0032009091339552063	4.3562668686964346E-4	4.545669776031062E-4	2.698991429518443E-4	4.11951323452815E-4	1.1837681708414225E-5
45-49	0.004216582224537147	4.498319049197405E-4	4.8771248638666603E-4	3.2435247881054967E-4	5.326956768786401E-4	1.1837681708414225E-5
50-54	0.005317486623419669	5.208579951702259E-4	5.255930678535915E-4	3.551304512524267E-4	6.794829300629764E-4	2.367536341682845E-5
55-59	0.006049055352999668	6.179269851792224E-4	5.634736493205169E-4	3.66968132960841E-4	8.025948198304844E-4	2.367536341682845E-5
60-64	0.00659122117524504	7.173635115299019E-4	5.847814763956626E-4	3.906434963776694E-4	9.067664188645295E-4	4.0248117808608366E-5
65-69	0.007033950471139731	8.096974288555329E-4	5.989866944457597E-4	4.1905393247786355E-4	0.0010748614991240115	5.918840854207112E-5
70-74	0.015216156067995642	8.830910554477012E-4	6.155594488375396E-4	4.900800227283489E-4	0.0011861357071831055	5.918840854207112E-5
75-79	0.16109190776078414	8.972962734977982E-4	6.605426393295137E-4	5.350632132203228E-4	0.0012571617974335906	5.918840854207112E-5
80-84	0.22293669207822336	9.564846820398693E-4	6.842180027463422E-4	5.587385766371514E-4	0.0014323594867181211	5.918840854207112E-5
85-89	0.22332496803825935	0.0010109380178985748	6.86585539088025E-4	6.060893034708082E-4	0.0017756522562621339	5.918840854207112E-5
90-94	0.22351910601827737	0.0010938017898574743	7.00790757138122E-4	6.297646668876366E-4	0.0018372082011458874	5.918840854207112E-5
95-99	0.2237369193617122	0.0011695629527913253	7.33936265921682E-4	6.889530754297078E-4	0.0018561484918793504	5.918840854207112E-5
100-104	0.223992613286614	0.0012311188976750795	7.694493110469247E-4	7.220985842132677E-4	0.001875088782612813	5.918840854207112E-5
105-109	0.22441403475543348	0.001266631942800322	7.931246744637529E-4	7.434064112884133E-4	0.0019200719731047873	5.918840854207112E-5
110-114	0.22470287418911883	0.0013565983237842703	8.144325015388987E-4	7.78919456413656E-4	0.001981627917988541	5.918840854207112E-5
115-119	0.22501538898622092	0.0014276244140347554	8.452104739807755E-4	8.2390264690563E-4	0.0020692267626308066	5.918840854207112E-5
120-124	0.22595293337752734	0.0015128557223353378	8.712533737392869E-4	8.665183010559211E-4	0.0021876035797149486	6.392348122543681E-5
125-129	0.2280316302855249	0.001572044130877409	9.091339552062124E-4	9.209716369146268E-4	0.0023580661963161138	7.102609025048534E-5
130-134	0.2302239689379232	0.0016430702211278944	9.351768549647237E-4	9.564846820398693E-4	0.002462237795350159	7.102609025048534E-5
135-139	0.23249443628959704	0.001709361238695014	9.351768549647237E-4	9.872626544817464E-4	0.0025758795397509355	7.102609025048534E-5
140-144	0.23508452104739805	0.001782754865287182	9.470145366731379E-4	0.0010275107722903546	0.0027084615748851747	7.102609025048534E-5
145-149	0.236921729248544	0.0018869264643212272	9.801600454566978E-4	0.0010961693261991572	0.002803163028552488	8.286377195889956E-5
150-154	0.23815284814621904	0.0019129693640797386	0.0010464510630238174	0.0011364174440077655	0.002886026800511388	8.286377195889956E-5
155-159	0.24001136417444008	0.0019437473365216157	0.0010606562810739145	0.001176665561816374	0.0029688905724702874	8.286377195889956E-5
160-164	0.242341019934656	0.002000568208722004	0.0010961693261991574	0.0012145461432832992	0.003047019271745821	8.286377195889956E-5
165-167	0.24431396688605836	0.0020439730416528562	0.001100904398882523	0.0012587401549947125	0.003069905456382089	9.864734757011855E-5
>>END_MODULE
