##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479119
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2105408
Total Bases	361.1 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.34029936240387	39.0	39.0	39.0	37.0	39.0
2	40.053970061859744	41.0	40.0	41.0	38.0	41.0
3	40.07657233182357	41.0	40.0	41.0	38.0	41.0
4	40.09144403365048	41.0	40.0	41.0	38.0	41.0
5	40.09638986837705	41.0	40.0	41.0	38.0	41.0
6	40.085737301273674	41.0	40.0	41.0	38.0	41.0
7	40.10211179970818	41.0	40.0	41.0	38.0	41.0
8	40.08972987658449	41.0	40.0	41.0	38.0	41.0
9	40.08031269948628	41.0	40.0	41.0	38.0	41.0
10-14	40.013401487977625	41.0	40.0	41.0	38.0	41.0
15-19	39.88999044365748	41.0	40.0	41.0	38.0	41.0
20-24	39.728566814603155	41.0	39.8	41.0	37.4	41.0
25-29	39.507514078031434	40.0	39.0	41.0	36.6	41.0
30-34	39.4225024318327	40.0	39.0	41.0	36.0	41.0
35-39	39.302641103292096	40.2	38.6	41.0	35.2	41.0
40-44	38.864351327628654	40.0	37.4	41.0	35.0	41.0
45-49	38.288482517402805	39.4	36.0	41.0	35.0	41.0
50-54	37.621091208924824	38.2	35.0	40.4	34.0	41.0
55-59	36.90648301896829	36.6	35.0	39.4	34.0	41.0
60-64	36.097884685533636	35.6	34.8	38.2	33.6	40.2
65-69	35.635625493966	35.0	35.0	36.8	33.4	39.0
70-74	35.30354126136122	35.0	35.0	36.0	33.0	38.0
75-79	35.059079760312486	35.0	35.0	35.4	33.0	37.0
80-84	34.9377626569292	35.0	35.0	35.0	33.0	36.6
85-89	34.75398241101012	35.0	34.6	35.0	32.6	36.2
90-94	39.558399012413275	40.6	39.8	40.6	37.8	40.6
95-99	39.826806833905565	41.0	40.0	41.0	37.6	41.0
100-104	39.728972611119886	41.0	40.0	41.0	37.0	41.0
105-109	39.58913199618874	41.0	39.0	41.0	36.4	41.0
110-114	39.41746400371868	40.4	39.0	41.0	35.8	41.0
115-119	39.181240871474344	40.0	38.2	41.0	35.0	41.0
120-124	38.913022732447715	40.0	38.0	41.0	35.0	41.0
125-129	38.452707751281736	39.6	37.2	40.6	34.6	41.0
130-134	38.296881838201834	39.8	36.4	41.0	34.0	41.0
135-139	37.98048804114015	39.0	35.0	41.0	34.0	41.0
140-144	37.502905013976495	37.8	35.0	40.6	34.0	41.0
145-149	36.79209676334839	36.4	35.0	39.2	34.0	41.0
150-154	36.08977463725751	35.2	35.0	37.6	34.0	40.0
155-159	35.48890010673817	35.0	35.0	36.4	33.4	38.6
160-164	35.033534673040194	35.0	35.0	35.8	33.0	37.0
165-169	34.743491556274456	35.0	35.0	35.0	33.0	36.0
170-174	34.57898623832557	35.0	35.0	35.0	33.0	36.0
175-178	34.287667409720555	35.0	34.25	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	15.0
30	131.0
31	1000.0
32	5032.0
33	18975.0
34	52911.0
35	124952.0
36	293891.0
37	595965.0
38	756725.0
39	252422.0
40	3389.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.094624889807584	28.40637064169985	27.45966577499468	22.039338693497886
2	21.829450633796394	28.891312277715294	28.620105936711553	20.65913115177676
3	20.746667629267108	27.623529501170317	29.32082522722437	22.308977642338206
4	21.061665957382132	26.94855343952336	30.028004073319753	21.961776529774752
5	21.802377496428246	27.389703088427513	28.880103048910232	21.927816366234005
6	22.41256801532055	27.231728957047757	27.996806319725202	22.358896707906496
7	22.19850024318327	27.831517691582818	27.86196309693893	22.10801896829498
8	22.17242453719184	27.86106065902666	27.97847258108642	21.98804222269508
9	22.694223637413746	27.67021878894732	27.22180214001277	22.413755433626168
10-14	22.386188330242877	28.011739292336685	27.4393466729489	22.162725704471534
15-19	22.375596558956744	27.952662856795452	27.508026947745996	22.163713636501807
20-24	22.200628096786943	27.92174248411709	27.697139936772352	22.180489482323615
25-29	22.1869300392133	28.049641684652094	27.578037131045384	22.185391145089216
30-34	22.290415919384746	28.054837827157492	27.4886767790376	22.166069474420162
35-39	22.238995957078153	28.069172340942945	27.576640727117972	22.11519097486093
40-44	22.175730309754687	28.109259582940695	27.62875414171505	22.08625596558957
45-49	22.38366150408852	28.018673815241513	27.44257645074019	22.15508822992978
50-54	22.310564033194517	28.034338237529262	27.536002523026415	22.11909520624981
55-59	22.256180274797092	28.081132018117156	27.545938839407846	22.116748867677906
60-64	22.30348702009302	28.052567483357144	27.500721950329815	22.14322354622002
65-69	22.25789015715719	28.059340517372405	27.552151411982855	22.13061791348755
70-74	22.16069284433231	28.197508511414416	27.582378332370734	22.059420311882544
75-79	22.203819877192448	28.15663282366173	27.478987445663737	22.16055985348208
80-84	22.198300756907923	28.20557345654619	27.50926186278384	22.086863923762046
85-89	22.22747324984041	28.21816009058577	27.444286333100283	22.11008032647354
90-94	21.340772037970076	27.729215696148167	28.943347968148615	21.98666429773314
95-99	22.035875467249834	27.641465965001377	27.98411029592529	22.3385482718235
100-104	22.15374282196081	27.79789309217186	27.692885911155464	22.355478174711866
105-109	21.92973047164191	27.801946558144824	28.012937193083363	22.255385777129895
110-114	22.0150627137129	27.615776895591438	27.99260826355427	22.376552127141395
115-119	21.974783974635837	27.68514501571312	27.964383590633496	22.37568741901754
120-124	21.89071788389373	27.775237611294635	27.999862821349442	22.334181683462194
125-129	21.936854224579257	27.744805522913364	27.965850199275128	22.35249005323226
130-134	21.98672295377282	27.72846072283624	27.941616607182283	22.34319971620865
135-139	21.981214470710633	27.794274914713803	27.95152678152677	22.272983833048798
140-144	22.065152484961402	27.76580414818351	27.932125850189593	22.236917516665493
145-149	21.989222182823365	27.793462600273767	28.01992666947266	22.197388547430204
150-154	21.85065043140001	27.929242499667932	28.110213119984657	22.109893948947406
155-159	21.886275649682847	27.936961050996285	28.06462038238699	22.112142916933884
160-164	21.80058493964437	28.085220192317028	28.092276227168643	22.021918640869952
165-169	21.68909603240057	28.21404684764423	28.145314415394484	21.951542704560715
170-174	21.751969557829863	28.27462487176504	28.111088078513056	21.86231749189204
175-178	21.65783268682565	28.396550923097834	28.123092017568574	21.82252437250794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.5
9	1.5
10	3.0
11	4.5
12	10.5
13	28.0
14	57.5
15	106.0
16	209.0
17	396.5
18	643.0
19	1025.5
20	1663.5
21	2529.0
22	3694.0
23	5296.5
24	7338.0
25	9723.0
26	12681.5
27	16025.0
28	19808.5
29	24075.0
30	28572.0
31	33607.5
32	39033.5
33	44476.0
34	50389.5
35	56453.5
36	62471.0
37	68561.5
38	73969.0
39	78911.0
40	83649.0
41	87216.5
42	89497.0
43	90884.0
44	90736.5
45	88958.5
46	86037.0
47	82312.0
48	77472.5
49	72008.0
50	66040.0
51	59881.0
52	54320.0
53	48912.5
54	43783.5
55	39380.0
56	35685.5
57	32585.0
58	29989.0
59	27668.0
60	25346.0
61	23088.5
62	21057.0
63	19115.0
64	16948.5
65	14718.0
66	12551.0
67	10510.0
68	8611.5
69	6769.0
70	5204.5
71	3894.0
72	2877.5
73	2093.5
74	1436.5
75	969.0
76	643.0
77	400.0
78	257.5
79	157.0
80	79.5
81	39.0
82	22.0
83	13.5
84	6.0
85	2.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	313.0
92-93	597.0
94-95	890.0
96-97	1162.0
98-99	1395.0
100-101	1702.0
102-103	1932.0
104-105	2184.0
106-107	2559.0
108-109	2676.0
110-111	2855.0
112-113	3035.0
114-115	3298.0
116-117	3340.0
118-119	3445.0
120-121	3713.0
122-123	3825.0
124-125	4134.0
126-127	4176.0
128-129	4609.0
130-131	4621.0
132-133	5105.0
134-135	22999.0
136-137	18674.0
138-139	18136.0
140-141	18353.0
142-143	18162.0
144-145	19428.0
146-147	19464.0
148-149	20245.0
150-151	19888.0
152-153	19475.0
154-155	18883.0
156-157	18899.0
158-159	19614.0
160-161	19937.0
162-163	20523.0
164-165	21241.0
166-167	21353.0
168-169	22549.0
170-171	23650.0
172-173	23924.0
174-175	24869.0
176-177	60718.0
178-179	1552858.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.59377091109181
#Duplication Level	Percentage of total
1	97.26559857311284
2	2.5283509322182627
3	0.16354200413595896
4	0.026836337266030283
5	0.008791561213119635
6	0.002249640688639364
7	0.0034532342111802897
8	0.0011777171540014453
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	4.749673222482293E-5	0.0	0.0	0.0
2	6.174575189226981E-4	0.0	4.749673222482293E-5	0.0	0.0	0.0
3	6.174575189226981E-4	0.0	4.749673222482293E-5	0.0	0.0	0.0
4	6.174575189226981E-4	0.0	4.749673222482293E-5	0.0	0.0	0.0
5	6.174575189226981E-4	9.499346444964586E-5	4.749673222482293E-5	0.0	0.0	0.0
6	6.174575189226981E-4	9.499346444964586E-5	4.749673222482293E-5	0.0	4.749673222482293E-5	0.0
7	6.174575189226981E-4	1.4249019667446878E-4	4.749673222482293E-5	0.0	4.749673222482293E-5	0.0
8	6.64954251147521E-4	1.4249019667446878E-4	9.499346444964586E-5	0.0	4.749673222482293E-5	0.0
9	6.64954251147521E-4	1.4249019667446878E-4	1.4249019667446878E-4	0.0	4.749673222482293E-5	0.0
10-14	6.64954251147521E-4	1.4249019667446878E-4	1.4249019667446878E-4	2.8498039334893758E-5	4.749673222482293E-5	0.0
15-19	7.029516369273794E-4	1.5198954311943338E-4	1.8048758245432713E-4	4.749673222482293E-5	4.749673222482293E-5	0.0
20-24	7.12450983372344E-4	1.8998692889929172E-4	2.75481046903973E-4	4.749673222482293E-5	4.749673222482293E-5	0.0
25-29	7.12450983372344E-4	2.659817004590084E-4	3.039790862388667E-4	1.0449281089461044E-4	7.599477155971667E-5	0.0
30-34	0.0011209228805058211	3.324771255737605E-4	3.324771255737605E-4	1.4249019667446878E-4	1.7098823600936256E-4	0.0
35-39	0.002498328115025686	3.324771255737605E-4	3.324771255737605E-4	1.4249019667446878E-4	3.324771255737605E-4	0.0
40-44	0.003752241845761012	3.514758184636897E-4	3.324771255737605E-4	1.6148888956439797E-4	5.224640544730523E-4	0.0
45-49	0.0046926771438125054	4.749673222482293E-4	3.514758184636897E-4	2.2798431467915007E-4	5.319634009180169E-4	0.0
50-54	0.00598458826032769	5.129647080280876E-4	3.7997385779858344E-4	2.3748366112411466E-4	6.079581724777334E-4	9.499346444964586E-6
55-59	0.006782533361704715	5.889594795878044E-4	3.7997385779858344E-4	2.75481046903973E-4	6.934522904824148E-4	9.499346444964585E-5
60-64	0.007333495455512662	6.174575189226981E-4	4.0847189713347725E-4	3.229777791287959E-4	8.834392193817065E-4	1.4249019667446878E-4
65-69	0.007741967352646137	6.459555582575919E-4	4.274705900234064E-4	3.324771255737605E-4	0.0011969176520655379	1.4249019667446878E-4
70-74	0.015445937319512415	7.78946408487096E-4	4.274705900234064E-4	3.324771255737605E-4	0.0014913973918594403	1.4249019667446878E-4
75-79	0.15873407909535825	8.549411800468128E-4	4.654679758032647E-4	3.324771255737605E-4	0.001747879745873484	1.4249019667446878E-4
80-84	0.21857046235219016	8.549411800468128E-4	4.749673222482293E-4	3.704745113536189E-4	0.002108854910782138	1.4249019667446878E-4
85-89	0.21894093686354382	8.549411800468128E-4	4.749673222482293E-4	3.7997385779858344E-4	0.0025648235401404384	1.4249019667446878E-4
90-94	0.2191784205246679	9.214366051615649E-4	4.749673222482293E-4	3.9897255068851263E-4	0.0025648235401404384	1.4249019667446878E-4
95-99	0.21955839438246647	9.974313767212816E-4	5.224640544730523E-4	4.274705900234064E-4	0.0025648235401404384	1.4249019667446878E-4
100-104	0.2201188558227194	0.0010639268018360337	6.174575189226981E-4	4.274705900234064E-4	0.0025648235401404384	1.4249019667446878E-4
105-109	0.22059382314496764	0.0011209228805058211	6.744535975924856E-4	4.4646928291333556E-4	0.0026123202723652614	1.4249019667446878E-4
110-114	0.22104979177432593	0.0011874183056205733	7.599477155971669E-4	5.604614402529107E-4	0.00263131896525519	1.4249019667446878E-4
115-119	0.22120178131744533	0.00126341307718029	7.599477155971669E-4	6.079581724777334E-4	0.002659817004590084	1.4249019667446878E-4
120-124	0.22242719700884578	0.0013204091558500772	7.694470620421316E-4	6.269568653676626E-4	0.0027548104690397297	1.4249019667446878E-4
125-129	0.2247355381949722	0.001377405234519865	8.549411800468128E-4	6.64954251147521E-4	0.002992294130163845	1.4249019667446878E-4
130-134	0.22724336565644282	0.0013869045809648296	8.549411800468128E-4	6.839529440374502E-4	0.003201279751953066	1.4249019667446878E-4
135-139	0.2297986898501383	0.0014818980454144755	8.834392193817065E-4	7.409490227072378E-4	0.003419764720187251	1.4249019667446878E-4
140-144	0.2324395081618385	0.0015198954311943338	9.499346444964586E-4	8.169437942669545E-4	0.0035527555704167554	1.4249019667446878E-4
145-149	0.23512782320576348	0.0015863908563090857	9.594339909414232E-4	8.92938565826671E-4	0.0036382496884214367	1.4249019667446878E-4
150-154	0.236315241511384	0.0016148888956439796	9.974313767212816E-4	9.024379122716357E-4	0.0036572483813113654	1.4249019667446878E-4
155-159	0.23796812779280785	0.001671884974313767	0.0010259294160561753	9.119372587166003E-4	0.003742742499316047	1.8048758245432713E-4
160-164	0.24079893303340727	0.001747879745873484	0.0010449281089461046	0.0010354287625011398	0.003942228774660304	1.8998692889929172E-4
165-167	0.24258164371624566	0.0017573790923184486	0.0010449281089461046	0.0010449281089461046	0.00405305448318489	1.8998692889929172E-4
>>END_MODULE
