##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479120
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8383240
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.24516094016156	39.0	39.0	39.0	37.0	39.0
2	39.995130880184746	41.0	40.0	41.0	38.0	41.0
3	40.05103957419804	41.0	40.0	41.0	38.0	41.0
4	40.06601147050544	41.0	40.0	41.0	38.0	41.0
5	40.067994832546844	41.0	40.0	41.0	38.0	41.0
6	40.06159169962926	41.0	40.0	41.0	38.0	41.0
7	40.08534528416221	41.0	40.0	41.0	38.0	41.0
8	40.06334364756347	41.0	40.0	41.0	38.0	41.0
9	40.0550521039598	41.0	40.0	41.0	38.0	41.0
10-14	39.993282573324876	41.0	40.0	41.0	38.0	41.0
15-19	39.877920135890186	41.0	40.0	41.0	38.0	41.0
20-24	39.70900596905254	41.0	39.6	41.0	37.2	41.0
25-29	39.49911392254069	40.0	39.0	41.0	36.6	41.0
30-34	39.420435368664144	40.0	39.0	41.0	36.0	41.0
35-39	39.28233069791632	40.0	38.6	41.0	35.0	41.0
40-44	38.84152802496409	40.0	37.4	41.0	35.0	41.0
45-49	38.26916764878495	39.4	36.0	41.0	35.0	41.0
50-54	37.619144793659736	38.2	35.0	40.4	34.0	41.0
55-59	36.919173589208945	36.6	35.0	39.4	34.0	41.0
60-64	36.14198116718596	35.6	34.8	38.4	33.8	40.2
65-69	35.67546175464379	35.0	35.0	37.0	33.0	39.0
70-74	35.361226781053624	35.0	35.0	36.0	33.0	38.6
75-79	35.120898173021416	35.0	35.0	35.6	33.0	37.0
80-84	35.00631645998444	35.0	35.0	35.0	33.0	37.0
85-89	34.82584728577495	35.0	34.6	35.0	32.6	37.2
90-94	39.50970375846763	40.6	39.8	40.6	37.6	40.6
95-99	39.79240238373497	41.0	40.0	41.0	37.0	41.0
100-104	39.68371758117405	41.0	39.6	41.0	37.0	41.0
105-109	39.545134675185956	41.0	39.0	41.0	36.2	41.0
110-114	39.364813928480956	40.2	39.0	41.0	35.6	41.0
115-119	39.12517516224248	40.0	38.0	41.0	35.0	41.0
120-124	38.856510499745234	40.0	38.0	41.0	35.0	41.0
125-129	38.42517594123315	39.6	37.0	40.6	34.6	41.0
130-134	38.23891769158565	39.6	36.2	41.0	34.0	41.0
135-139	37.916837609143194	39.0	35.0	41.0	34.0	41.0
140-144	37.443654931971494	37.6	35.0	40.4	34.0	41.0
145-149	36.75471777448921	36.4	35.0	39.2	34.0	41.0
150-154	36.06144755450669	35.2	35.0	37.6	34.0	39.8
155-159	35.46700255395227	35.0	35.0	36.4	33.2	38.6
160-164	35.02137563204947	35.0	35.0	35.8	33.0	37.0
165-169	34.73482055206493	35.0	35.0	35.0	33.0	36.0
170-174	34.56914199419121	35.0	35.0	35.0	33.0	36.0
175-178	34.26956050100529	35.0	33.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	53.0
30	531.0
31	4003.0
32	20442.0
33	76297.0
34	215114.0
35	506451.0
36	1178040.0
37	2363464.0
38	2989269.0
39	1013479.0
40	16097.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.059883768089662	28.45903254588918	27.444663399831093	22.036420286190065
2	21.76140728405724	28.938143247718067	28.61943592214943	20.681013546075263
3	20.74200428473955	27.62175483464627	29.34599271880562	22.29024816180856
4	21.04506133666697	26.985354111298253	30.006524923537913	21.963059628496858
5	21.76774135059953	27.426078699882144	28.905315844470636	21.90086410504769
6	22.416511992976464	27.248295408457828	28.062455566105704	22.272737032460004
7	22.118787008364308	27.846107233002993	27.942144087488845	22.092961671143854
8	22.14072363429891	27.87635806680949	28.01480096001069	21.96811733888091
9	22.69493656390608	27.65180288289492	27.257575829870074	22.395684723328927
10-14	22.377601022993495	28.042997695401777	27.455740262714656	22.12366101889007
15-19	22.357250895835023	27.961830986587525	27.564805492864338	22.116112624713118
20-24	22.206361740806656	27.943518257857342	27.747450866252187	22.10266913508381
25-29	22.168323941578674	28.075023499267587	27.610973800105924	22.145678759047815
30-34	22.279894169795927	28.091370400942832	27.50913727866553	22.119598150595714
35-39	22.195783491824166	28.09740625342946	27.608473573463243	22.098336681283133
40-44	22.148930485110768	28.16030794776244	27.64622508719779	22.044536479929
45-49	22.343960091802213	28.04038295456172	27.4915593493685	22.12409760426756
50-54	22.28446280912869	28.06633712025422	27.565688206469098	22.08351186414799
55-59	22.209606309732273	28.10364966289883	27.610980957243264	22.07576307012563
60-64	22.27119347650789	28.059122725819613	27.560217767832007	22.10946602984049
65-69	22.206099312437676	28.07170497325616	27.61392969782566	22.108266016480503
70-74	22.11460724016013	28.203040829082788	27.631631684169843	22.05072024658724
75-79	22.19873461811901	28.14788077163483	27.545235493675474	22.108149116570683
80-84	22.17847276232101	28.201843201435246	27.543873251869204	22.07581078437454
85-89	22.176103749862822	28.251377748937166	27.506317366555173	22.06620113464484
90-94	21.321223344643983	27.748102187854663	28.944501614656144	21.98617285284521
95-99	22.01050087555868	27.674249745845998	28.01248633201134	22.302763046583983
100-104	22.12304690697973	27.800550038793993	27.74688812612706	22.32951492809921
105-109	21.932609510014363	27.81210871446747	28.025358879564216	22.22992289595395
110-114	21.96534034038556	27.673526875324267	28.014377418808095	22.34675536548208
115-119	21.9526479151628	27.72301009058336	27.961597238073733	22.36274475618011
120-124	21.863468993567437	27.8103925672482	28.020678735584113	22.30545970360025
125-129	21.915118027542746	27.76818813066481	27.98955982358613	22.327134018206312
130-134	21.956712137267854	27.756287970697883	27.973486587594802	22.313513304439457
135-139	21.989257706726136	27.799779151119303	27.959829421349163	22.251133720805395
140-144	22.02961238753672	27.817265967077127	27.94333499372484	22.209786651661314
145-149	21.953030540196718	27.82646090928812	28.028832381388842	22.191676169126318
150-154	21.839363036508047	27.948547215347517	28.12934118150873	22.082748566635697
155-159	21.86796866733665	27.97015243573843	28.082337091090025	22.07954180583489
160-164	21.770206311680234	28.102429688220358	28.13839470379436	21.988969296305044
165-169	21.650812701550258	28.230275438887865	28.188999529341892	21.929912330219988
170-174	21.717239896479377	28.31905593683351	28.12532595584153	21.838378210845587
175-178	21.658346663126913	28.389038478907157	28.16065623549287	21.791958622473057
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.5
6	1.0
7	1.5
8	3.0
9	4.0
10	11.5
11	34.0
12	65.5
13	118.0
14	219.0
15	443.0
16	807.0
17	1418.0
18	2566.0
19	4354.5
20	6821.0
21	10384.5
22	15212.5
23	21455.0
24	29521.0
25	39265.0
26	50715.5
27	64080.5
28	79462.0
29	96576.0
30	115002.0
31	135084.5
32	156439.0
33	178667.5
34	201934.0
35	225984.5
36	249969.0
37	273137.0
38	295849.5
39	316521.0
40	334295.5
41	348191.5
42	357460.0
43	361578.5
44	360201.5
45	354157.0
46	343093.5
47	327068.0
48	307323.5
49	286128.0
50	263015.5
51	238651.0
52	215383.5
53	193410.5
54	173648.5
55	156382.5
56	141729.0
57	129229.0
58	118170.5
59	108562.0
60	99879.0
61	91630.5
62	83243.0
63	74816.5
64	66211.0
65	57486.5
66	49028.5
67	40893.0
68	33332.5
69	26553.5
70	20601.5
71	15598.5
72	11306.0
73	8063.5
74	5720.0
75	3819.0
76	2464.0
77	1562.0
78	923.5
79	516.0
80	306.5
81	163.0
82	88.0
83	49.0
84	21.5
85	10.0
86	4.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1261.0
92-93	2756.0
94-95	3929.0
96-97	5293.0
98-99	6635.0
100-101	8453.0
102-103	8268.0
104-105	10349.0
106-107	10816.0
108-109	11593.0
110-111	12214.0
112-113	13442.0
114-115	15975.0
116-117	14493.0
118-119	15683.0
120-121	16482.0
122-123	16419.0
124-125	17899.0
126-127	18538.0
128-129	22712.0
130-131	21290.0
132-133	22584.0
134-135	98793.0
136-137	87660.0
138-139	95735.0
140-141	80078.0
142-143	83866.0
144-145	80609.0
146-147	86308.0
148-149	88151.0
150-151	86935.0
152-153	80603.0
154-155	82618.0
156-157	97048.0
158-159	87212.0
160-161	81476.0
162-163	82859.0
164-165	91924.0
166-167	87395.0
168-169	88523.0
170-171	99765.0
172-173	100701.0
174-175	101789.0
176-177	251367.0
178-179	5984741.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.21931925125318
#Duplication Level	Percentage of total
1	91.06957262622554
2	7.386067754487942
3	1.0639338209875044
4	0.2645225055884955
5	0.0982529718376138
6	0.05147832731679207
7	0.024856524421429903
8	0.01354384675588201
9	0.0062880245160285555
>10	0.02148359786279946
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.578568667961313E-5	1.192856222653771E-5	1.192856222653771E-5	0.0	0.0	0.0
2	8.707850425372529E-4	1.192856222653771E-5	3.578568667961313E-5	2.385712445307542E-5	0.0	0.0
3	8.827136047637906E-4	2.385712445307542E-5	7.157137335922626E-5	3.578568667961313E-5	0.0	0.0
4	8.827136047637906E-4	3.578568667961313E-5	9.542849781230168E-5	3.578568667961313E-5	1.192856222653771E-5	0.0
5	8.946421669903283E-4	5.964281113268855E-5	1.073570600388394E-4	3.578568667961313E-5	1.192856222653771E-5	0.0
6	8.946421669903283E-4	5.964281113268855E-5	1.073570600388394E-4	5.964281113268855E-5	1.192856222653771E-5	0.0
7	8.946421669903283E-4	8.349993558576398E-5	1.073570600388394E-4	5.964281113268855E-5	1.192856222653771E-5	0.0
8	9.184992914434037E-4	9.542849781230168E-5	1.073570600388394E-4	5.964281113268855E-5	1.192856222653771E-5	0.0
9	9.184992914434037E-4	1.073570600388394E-4	1.073570600388394E-4	7.157137335922626E-5	2.385712445307542E-5	0.0
10-14	9.232707163340188E-4	1.2644275960129974E-4	1.1451419737476202E-4	8.349993558576398E-5	3.339997423430559E-5	0.0
15-19	9.423564158964791E-4	1.7177129606214304E-4	1.5984273383560534E-4	1.0497134759353186E-4	5.487138624207347E-5	0.0
20-24	9.542849781230168E-4	2.0755698274175618E-4	1.7415700850745058E-4	1.1212848492945448E-4	8.827136047637906E-5	0.0
25-29	9.662135403495547E-4	2.4334266942136932E-4	1.8608557073398829E-4	1.2167133471068465E-4	1.240570471559922E-4	0.0
30-34	0.0014027989178408348	3.1491404278059554E-4	1.9562842051521848E-4	1.4075703427314499E-4	2.1232840763237126E-4	0.0
35-39	0.002662455088963217	4.079568281475897E-4	2.2187125741360142E-4	1.5029988405437515E-4	3.196854676712107E-4	4.771424890615084E-6
40-44	0.0037932827880389915	4.7475677661620093E-4	3.005997681087503E-4	1.8847128317929583E-4	4.294282401553576E-4	1.4314274671845253E-5
45-49	0.005155524594309598	5.272424504129668E-4	3.2922831745244084E-4	2.0517127029644864E-4	5.463281499754272E-4	3.101426178899805E-5
50-54	0.006577409211712894	5.630281370925799E-4	3.506997294602087E-4	2.600426565385221E-4	7.204851584828777E-4	3.578568667961313E-5
55-59	0.007705851198343362	6.035852486628081E-4	3.745568539132841E-4	2.838997809915975E-4	8.683993300919454E-4	3.578568667961313E-5
60-64	0.008443036343943392	6.345995104518063E-4	4.079568281475897E-4	3.38771167233671E-4	0.0010592563257165485	3.817139912492067E-5
65-69	0.009087178704176429	6.703851971314194E-4	4.1988539037412744E-4	4.1749967792881985E-4	0.001204784784880309	5.964281113268855E-5
70-74	0.017038758284386467	6.990137464751098E-4	4.628282143896632E-4	4.6998535172558585E-4	0.0013526989564893765	6.202852357799609E-5
75-79	0.16189921796346043	7.634279824984136E-4	4.79528201506816E-4	5.391710126395044E-4	0.0014719845787547535	7.157137335922626E-5
80-84	0.22244621411292057	8.278422185217172E-4	5.009996135145838E-4	5.94042398881578E-4	0.0016890844112777401	9.304278536699414E-5
85-89	0.22288041377796652	8.779421798731755E-4	5.200853130770442E-4	6.417566477877289E-4	0.0020087698789489506	9.542849781230168E-5
90-94	0.22325019920698916	8.994135918809433E-4	5.343995877488893E-4	6.679994846861118E-4	0.002015927016284873	9.542849781230168E-5
95-99	0.2236128274986759	9.113421541074812E-4	5.36785300194197E-4	6.990137464751099E-4	0.002027855578511411	9.542849781230168E-5
100-104	0.22398261292769864	9.566706905683245E-4	5.797281242097327E-4	7.538851327171832E-4	0.0020540984154097937	9.542849781230168E-5
105-109	0.22439295546829152	0.0010067706519197828	6.202852357799609E-4	8.039850940686417E-4	0.002073184114972254	9.542849781230168E-5
110-114	0.2248724836697983	0.0010353992012634736	6.46528072678344E-4	8.111422314045644E-4	0.0021137412265424824	9.542849781230168E-5
115-119	0.2253710975708676	0.0010544849008259337	6.942423215844948E-4	8.135279438498718E-4	0.0021638411878939405	9.542849781230168E-5
120-124	0.22659019663041974	0.0011165134244039297	7.347994331547229E-4	8.302279309670247E-4	0.002256883973260935	9.542849781230168E-5
125-129	0.2290689518610943	0.0011976276475443863	7.562708451624908E-4	8.683993300919454E-4	0.0023737838830810046	9.542849781230168E-5
130-134	0.23177434977407302	0.0012477276088958445	7.848993945061813E-4	9.137278665527886E-4	0.002447740968885538	9.542849781230168E-5
135-139	0.23414336223226342	0.001266813308458305	7.920565318421039E-4	9.63827827904247E-4	0.0025360123293619173	9.542849781230168E-5
140-144	0.23692748865593732	0.0013670132311612217	8.039850940686417E-4	9.924563772479376E-4	0.002657683664072602	9.781421025760924E-5
145-149	0.23943725814840086	0.0014767560036453686	8.206850811857946E-4	0.0010759563128337015	0.0027364121747677508	1.073570600388394E-4
150-154	0.24101898549963977	0.0015435559521139797	8.421564931935625E-4	0.0011332134115210825	0.0028533120845878205	1.073570600388394E-4
155-159	0.2430516125030418	0.0016031987632466685	8.731707549825606E-4	0.001161841960864773	0.0029749834192985053	1.073570600388394E-4
160-164	0.2452822536394043	0.0016532987245981268	9.089564416621735E-4	0.0012167133471068464	0.0031038118913451125	1.073570600388394E-4
165-167	0.2469649761508279	0.001689879648759509	9.184992914434037E-4	0.0012644275960129975	0.003157092802623647	1.1530943485653121E-4
>>END_MODULE
