##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479121
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1853292
Total Bases	318.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3891378153038	39.0	39.0	39.0	37.0	39.0
2	40.10805852504624	41.0	40.0	41.0	38.0	41.0
3	40.12637080395318	41.0	40.0	41.0	38.0	41.0
4	40.13118062345276	41.0	40.0	41.0	38.0	41.0
5	40.13783580784895	41.0	40.0	41.0	38.0	41.0
6	40.1268823261526	41.0	40.0	41.0	38.0	41.0
7	40.155241591718955	41.0	40.0	41.0	38.0	41.0
8	40.13041873595742	41.0	40.0	41.0	38.0	41.0
9	40.122447515016525	41.0	40.0	41.0	38.0	41.0
10-14	40.06274542813544	41.0	40.0	41.0	38.0	41.0
15-19	39.938894788301035	41.0	40.0	41.0	38.0	41.0
20-24	39.77433065053968	41.0	40.0	41.0	37.6	41.0
25-29	39.56325392868474	40.4	39.0	41.0	36.8	41.0
30-34	39.48453961922892	40.2	39.0	41.0	36.0	41.0
35-39	39.3612085952996	40.6	38.8	41.0	35.2	41.0
40-44	38.92085316291227	40.0	37.4	41.0	35.0	41.0
45-49	38.3410336849239	39.4	36.0	41.0	35.0	41.0
50-54	37.66626047055726	38.2	35.0	40.6	34.4	41.0
55-59	36.94265587937573	36.6	35.0	39.4	34.0	41.0
60-64	36.134072450536664	35.6	34.8	38.2	33.6	40.2
65-69	35.64972740399246	35.0	35.0	36.8	33.6	39.0
70-74	35.317738381215705	35.0	35.0	36.0	33.6	38.0
75-79	35.073273936325194	35.0	35.0	35.4	33.0	37.0
80-84	34.946047573722865	35.0	35.0	35.0	33.0	36.2
85-89	34.75505295441841	35.0	34.6	35.0	32.6	36.0
90-94	39.61820713710024	40.6	39.8	40.6	37.8	40.6
95-99	39.89220530632066	41.0	40.0	41.0	38.0	41.0
100-104	39.78586049434682	41.0	40.0	41.0	37.2	41.0
105-109	39.65561629850528	41.0	39.6	41.0	36.8	41.0
110-114	39.480836895855845	40.6	39.0	41.0	36.0	41.0
115-119	39.242371749734765	40.0	38.4	41.0	35.2	41.0
120-124	38.97268829668799	40.0	38.0	41.0	35.0	41.0
125-129	38.50804372904746	39.6	37.4	40.6	34.6	41.0
130-134	38.3538226357	39.8	36.4	41.0	34.0	41.0
135-139	38.030386937782616	39.0	35.2	41.0	34.0	41.0
140-144	37.5409584559185	37.8	35.0	40.6	34.0	41.0
145-149	36.82934382838747	36.4	35.0	39.2	34.0	41.0
150-154	36.116629661381396	35.4	35.0	37.6	34.0	40.0
155-159	35.50931470300323	35.0	35.0	36.4	34.0	38.6
160-164	35.04903568408129	35.0	35.0	35.8	33.0	37.0
165-169	34.761300607340175	35.0	35.0	35.0	33.0	36.0
170-174	34.59054612516282	35.0	35.0	35.0	33.0	36.0
175-178	34.29388160955076	35.0	34.25	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	9.0
30	96.0
31	734.0
32	3866.0
33	14762.0
34	43194.0
35	103375.0
36	249421.0
37	525223.0
38	680465.0
39	229162.0
40	2985.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.04854928419267	28.441605532209714	27.436583118040765	22.073262065556857
2	21.80719498060748	28.951023368147062	28.531553581410808	20.710228069834653
3	20.818198103698716	27.58124461768572	29.33633771688433	22.26421956173123
4	21.014875151891875	26.95009744821647	30.022683959138657	22.012343440752996
5	21.78469447879773	27.418237385150316	28.901112183077466	21.895955952974493
6	22.45048270860717	27.252802040908826	27.958627134849774	22.338088115634232
7	22.130079879479325	27.847635450862573	27.90774470509774	22.11453996456036
8	22.215495453495727	27.776141050627746	27.946756366508897	22.061607129367633
9	22.724265792978116	27.601964504244343	27.223772616511592	22.449997086265952
10-14	22.411589754879422	28.001977022509134	27.39762541466752	22.18880780794392
15-19	22.392887898938753	27.931993447335874	27.52298072834718	22.15213792537819
20-24	22.242032016541376	27.91789960783298	27.687941241855036	22.15212713377061
25-29	22.212560136233254	28.044096666904082	27.551060491277145	22.19228270558552
30-34	22.327307299659203	28.063338103223884	27.419586336098146	22.18976826101877
35-39	22.218873226669082	28.061827278162323	27.540214925656613	22.179084569511982
40-44	22.18960638690503	28.121396952018358	27.60365878663481	22.085337874441805
45-49	22.388841046095273	28.009520356209382	27.42527351329418	22.176365084401162
50-54	22.32095104279304	28.039693691010374	27.493627555722465	22.14572771047412
55-59	22.241643518668404	28.06064020132823	27.566050034209393	22.131666245793973
60-64	22.324943937598608	28.013696708343854	27.500588142613253	22.16077121144428
65-69	22.25415099185665	28.04511107801685	27.532887424108022	22.16785050601848
70-74	22.14989327100101	28.206078696719132	27.549948955696134	22.09407907658372
75-79	22.252208502491783	28.120166708753935	27.45238203154171	22.175242757212573
80-84	22.246888239953552	28.192319397051303	27.44590706699214	22.114885296003003
85-89	22.24696378120663	28.22255748149779	27.41198904435998	22.118489692935597
90-94	21.363306969433808	27.68175990740147	28.950575189785322	22.0043579333794
95-99	22.085079531345357	27.612631459771304	27.965506330173728	22.336782678709614
100-104	22.17657899964984	27.772325310523723	27.700399632786247	22.35069605704019
105-109	22.011967973907463	27.772096354196325	27.973243694460383	22.242691977435836
110-114	22.03420804759456	27.626168785474746	27.945259987971262	22.39436317895943
115-119	22.004451630134216	27.661872572660425	27.914032654528683	22.41964314267668
120-124	21.92887870812406	27.772580006412657	27.968696339768123	22.32984494569516
125-129	21.96238274946499	27.740694280123186	27.908205658052033	22.388717312359795
130-134	22.02091680062179	27.714611482945433	27.89875437499772	22.365717341435058
135-139	22.023039075682167	27.783246874343792	27.914865297051843	22.278848752922197
140-144	22.06964289513772	27.778915752373408	27.878561689564492	22.272879662924385
145-149	22.019215585587983	27.761157886586812	27.978748765372973	22.24087776245223
150-154	21.86209948642134	27.90428794169393	28.088592620818808	22.145019951065926
155-159	21.923419520130658	27.91865977857736	28.012838796070604	22.14508190522138
160-164	21.82515790768981	28.0342062498335	28.086599000097685	22.05403684237901
165-169	21.681519119828206	28.168486002457392	28.172335873160463	21.97765900455394
170-174	21.788257287647884	28.26029828186326	28.087368759807468	21.864075670681384
175-178	21.680539582009498	28.38417734924911	28.07871063286752	21.856572435873872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	4.0
12	13.0
13	28.5
14	51.0
15	96.5
16	190.5
17	328.0
18	536.0
19	915.5
20	1489.5
21	2258.0
22	3333.0
23	4725.0
24	6377.5
25	8420.0
26	10904.5
27	13878.0
28	17312.5
29	20984.5
30	24904.5
31	29271.5
32	34089.5
33	39102.5
34	44177.5
35	49611.0
36	54962.0
37	59909.0
38	64900.0
39	69555.0
40	73599.5
41	76876.0
42	79026.5
43	79835.0
44	79648.0
45	78628.0
46	76266.5
47	72454.0
48	68107.5
49	63332.5
50	58225.0
51	53126.5
52	47700.5
53	42901.0
54	38740.5
55	34755.5
56	31596.5
57	28919.5
58	26423.0
59	24477.5
60	22426.0
61	20397.5
62	18694.0
63	16916.5
64	14993.0
65	13009.5
66	11149.5
67	9288.5
68	7501.0
69	5948.5
70	4634.5
71	3558.0
72	2611.0
73	1831.0
74	1260.0
75	851.5
76	592.5
77	374.0
78	206.5
79	119.0
80	64.0
81	33.5
82	18.5
83	8.5
84	4.5
85	4.0
86	2.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	254.0
92-93	480.0
94-95	762.0
96-97	926.0
98-99	1186.0
100-101	1325.0
102-103	1562.0
104-105	1742.0
106-107	1991.0
108-109	2181.0
110-111	2252.0
112-113	2334.0
114-115	2521.0
116-117	2713.0
118-119	2849.0
120-121	3057.0
122-123	3144.0
124-125	3185.0
126-127	3411.0
128-129	3665.0
130-131	3759.0
132-133	4027.0
134-135	19233.0
136-137	15589.0
138-139	14949.0
140-141	15149.0
142-143	15445.0
144-145	15721.0
146-147	16324.0
148-149	16456.0
150-151	16711.0
152-153	16079.0
154-155	15713.0
156-157	16125.0
158-159	16913.0
160-161	16927.0
162-163	17229.0
164-165	17606.0
166-167	18462.0
168-169	19064.0
170-171	20103.0
172-173	20623.0
174-175	21544.0
176-177	54377.0
178-179	1387624.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.7477832494415
#Duplication Level	Percentage of total
1	97.55331748973836
2	2.290265357891455
3	0.13507358639112796
4	0.009755983338124491
5	0.004452924065054607
6	0.0011429596209058678
7	0.004692729149857593
8	0.0
9	0.0
>10	0.001298969805101148
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.0791607582615153E-4	0.0	0.0	0.0	0.0	0.0
2	7.554125307830606E-4	0.0	0.0	1.0791607582615153E-4	5.395803791307576E-5	0.0
3	7.554125307830606E-4	0.0	0.0	1.0791607582615153E-4	5.395803791307576E-5	0.0
4	7.554125307830606E-4	0.0	0.0	1.0791607582615153E-4	5.395803791307576E-5	0.0
5	7.554125307830606E-4	0.0	0.0	1.0791607582615153E-4	5.395803791307576E-5	0.0
6	7.554125307830606E-4	0.0	0.0	1.6187411373922727E-4	5.395803791307576E-5	0.0
7	7.554125307830606E-4	0.0	0.0	1.6187411373922727E-4	5.395803791307576E-5	0.0
8	7.554125307830606E-4	0.0	0.0	1.6187411373922727E-4	5.395803791307576E-5	0.0
9	7.554125307830606E-4	0.0	0.0	1.6187411373922727E-4	5.395803791307576E-5	0.0
10-14	7.554125307830606E-4	0.0	2.1583215165230305E-5	1.6187411373922727E-4	5.395803791307576E-5	0.0
15-19	7.554125307830606E-4	1.0791607582615153E-5	9.712446824353637E-5	1.6187411373922727E-4	5.395803791307576E-5	0.0
20-24	8.417453914439819E-4	5.395803791307576E-5	1.8345732890445759E-4	2.1583215165230305E-4	5.395803791307576E-5	0.0
25-29	9.712446824353637E-4	5.395803791307576E-5	2.1583215165230305E-4	3.2374822747845453E-4	5.395803791307576E-5	0.0
30-34	0.0013705341629921242	1.1870768340876666E-4	2.1583215165230305E-4	3.2374822747845453E-4	1.618741137392273E-4	0.0
35-39	0.0023309872378448728	1.8345732890445759E-4	2.1583215165230305E-4	3.2374822747845453E-4	4.316643033046061E-4	5.395803791307576E-5
40-44	0.003356189958193312	2.482069744001485E-4	2.1583215165230305E-4	3.2374822747845453E-4	6.043300246264484E-4	5.395803791307576E-5
45-49	0.0046619744756897456	4.100810881393758E-4	2.2662375923491817E-4	3.2374822747845453E-4	8.093705686961364E-4	5.395803791307576E-5
50-54	0.005816676487029567	4.5324751846983645E-4	2.8058179714799395E-4	3.992894805567606E-4	9.712446824353637E-4	5.395803791307576E-5
55-59	0.006701588308804009	4.8562234121768183E-4	3.2374822747845453E-4	4.316643033046061E-4	0.0011978684416702819	7.554125307830606E-5
60-64	0.007370667978926148	5.179971639655273E-4	3.2374822747845453E-4	4.316643033046061E-4	0.0014676586312356607	1.4029089857399698E-4
65-69	0.007802332282230755	6.151216322090637E-4	3.2374822747845453E-4	4.8562234121768183E-4	0.0017374488208010395	1.6187411373922727E-4
70-74	0.015615456172044125	6.690796701221394E-4	3.453314426436849E-4	4.8562234121768183E-4	0.0019640725800359575	1.6187411373922727E-4
75-79	0.16376264506618493	7.014544928699849E-4	4.316643033046061E-4	4.8562234121768183E-4	0.002125946693775185	2.050405440696879E-4
80-84	0.22633238583018755	7.014544928699849E-4	4.8562234121768183E-4	4.8562234121768183E-4	0.0023633620605927183	2.1583215165230305E-4
85-89	0.2267640501334922	7.014544928699849E-4	4.8562234121768183E-4	4.8562234121768183E-4	0.002708693503236403	2.1583215165230305E-4
90-94	0.22700146550030972	7.769957459482909E-4	4.8562234121768183E-4	4.8562234121768183E-4	0.0027734431487320944	2.1583215165230305E-4
95-99	0.22713096479130107	8.633286066092122E-4	4.8562234121768183E-4	4.8562234121768183E-4	0.002859776009393015	2.1583215165230305E-4
100-104	0.22731442212020556	8.633286066092122E-4	4.96413948800297E-4	4.8562234121768183E-4	0.0028921508321408606	2.1583215165230305E-4
105-109	0.22767054517043187	8.849118217744425E-4	5.935384170438334E-4	5.503719867133727E-4	0.002913734047306091	2.1583215165230305E-4
110-114	0.22809141786615386	0.0010252027203484393	5.935384170438334E-4	6.259132397916788E-4	0.002978483692801782	2.1583215165230305E-4
115-119	0.22829645841022353	0.0010683691506789	6.259132397916788E-4	6.798712777047547E-4	0.0030648165534627033	2.1583215165230305E-4
120-124	0.2292029534471632	0.0010791607582615152	7.230377080352151E-4	7.769957459482909E-4	0.003075608161045318	2.1583215165230305E-4
125-129	0.23086486101488593	0.001133118796174591	7.554125307830606E-4	8.633286066092122E-4	0.003075608161045318	2.1583215165230305E-4
130-134	0.23317426503756558	0.0011870768340876668	8.201621762787516E-4	9.17286644522288E-4	0.003118774591375779	2.1583215165230305E-4
135-139	0.2351707124403494	0.0012410348720007425	8.957034293570577E-4	0.0010252027203484393	0.0032698570975323907	2.1583215165230305E-4
140-144	0.23704845215972442	0.0012734096947485877	9.388698596875182E-4	0.001133118796174591	0.003388564780941158	2.1583215165230305E-4
145-149	0.23948735547339547	0.0013705341629921242	9.712446824353637E-4	0.001133118796174591	0.0035828137174282308	2.1583215165230305E-4
150-154	0.2408794728515528	0.0014352838084878152	9.712446824353637E-4	0.0011978684416702819	0.003766271046332688	2.1583215165230305E-4
155-159	0.24243346434344942	0.0014568670236530456	9.712446824353637E-4	0.0012410348720007425	0.003992894805567606	2.1583215165230305E-4
160-164	0.24476445158129426	0.0014568670236530456	0.0010467859355136697	0.0012410348720007425	0.004057644451063297	2.1583215165230305E-4
165-167	0.24721774370507543	0.0014568670236530456	0.0010791607582615152	0.0012410348720007425	0.004100810881393757	2.1583215165230305E-4
>>END_MODULE
