##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479140
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12288947
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.108731691983046	39.0	38.0	39.0	36.0	39.0
2	39.716830660918305	40.0	40.0	41.0	38.0	41.0
3	39.73646562231898	40.0	40.0	41.0	38.0	41.0
4	39.7174446272736	40.0	40.0	41.0	38.0	41.0
5	39.70176386959761	40.0	40.0	41.0	38.0	41.0
6	39.66361723262376	40.0	39.0	41.0	37.0	41.0
7	39.6746652093137	40.0	40.0	41.0	37.0	41.0
8	39.65287009537921	40.0	39.0	41.0	37.0	41.0
9	39.61734475704061	40.0	39.0	41.0	37.0	41.0
10-14	39.4793995775228	40.0	39.0	41.0	37.0	41.0
15-19	39.367825851962735	40.0	39.0	41.0	36.6	41.0
20-24	39.457569228673535	40.0	39.0	41.0	37.0	41.0
25-29	39.34874174329176	40.0	39.0	41.0	36.4	41.0
30-34	39.109362909613004	40.0	38.2	41.0	35.6	41.0
35-39	38.75001155103037	40.0	38.0	41.0	34.8	41.0
40-44	38.2356489616238	39.6	36.8	40.6	34.0	41.0
45-49	37.56284104732488	38.8	35.6	40.0	33.0	41.0
50-54	36.91930735806737	37.6	35.0	40.0	32.6	41.0
55-59	36.31867986736374	36.6	34.2	39.0	32.0	40.6
60-64	35.666841105263124	35.8	33.8	38.8	31.4	40.0
65-69	35.55333212845657	35.0	34.0	38.0	31.8	40.0
70-74	35.470272806937814	35.0	34.0	38.0	31.6	40.0
75-79	35.4595619624692	35.0	34.0	38.0	31.0	40.6
80-84	35.59024988878217	35.0	34.0	39.0	31.0	41.0
85-89	35.727772737566525	35.0	33.6	39.4	30.6	41.0
90-94	39.00465830466242	39.8	39.0	40.4	36.4	41.0
95-99	39.360259912802825	40.0	39.0	41.0	36.0	41.0
100-104	39.15413895974475	40.0	38.2	41.0	35.4	41.0
105-109	39.02035318310292	40.0	38.0	41.0	35.0	41.0
110-114	38.868097860963175	40.0	38.0	41.0	35.0	41.0
115-119	38.6701142048101	40.0	37.6	41.0	34.2	41.0
120-124	38.390863202412795	40.0	36.8	41.0	34.0	41.0
125-129	38.07784956725675	39.6	36.0	41.0	33.8	41.0
130-134	37.58277751646584	38.8	35.0	40.4	33.0	41.0
135-139	37.425387925205776	38.6	35.0	40.6	33.0	41.0
140-144	36.92213415423255	37.2	35.0	40.0	33.0	41.0
145-149	36.35449992668776	36.2	35.0	39.2	32.4	41.0
150-154	35.74323745614912	35.2	34.0	38.0	32.0	40.2
155-159	35.12896102426588	35.0	34.0	36.6	31.8	39.0
160-164	34.58511793129533	35.0	34.0	35.8	31.0	37.2
165-169	34.17600797573711	35.0	34.0	35.0	31.0	36.4
170-174	33.874660107428454	35.0	33.4	35.0	31.0	36.0
175-179	33.58271820452739	34.2	33.0	35.0	30.2	35.2
180	32.2727124805844	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	71.0
31	1239.0
32	12784.0
33	74148.0
34	295153.0
35	893744.0
36	2298916.0
37	4347583.0
38	3641850.0
39	716119.0
40	7338.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.082644428363146	28.40135123050006	28.692490902597267	21.82351343853953
2	20.665448390329942	28.06222534770473	30.257466323192705	21.014859938772624
3	20.826259564794285	27.68960595240585	29.71101592349613	21.77311855930374
4	20.711400252600974	27.88779217617262	29.89675193488913	21.50405563633727
5	20.950721001563437	28.047724512116456	29.6553154635625	21.346239022757604
6	21.634229523489683	27.891356354616875	28.934081984404358	21.54033213748908
7	21.15392799724826	28.499911343095548	29.13373293903863	21.212427720617562
8	20.949598041231685	28.405924445764146	29.482770167370724	21.161707345633438
9	21.51429247762237	28.27926591269374	28.637791342089763	21.568650267594123
10-14	21.083218928358953	28.692411156138924	28.923011874003524	21.30135804149859
15-19	21.15061119557274	28.736325415025387	28.712767660239724	21.40029572916215
20-24	21.0914930302816	28.752310511226064	28.811741152435598	21.344455306056737
25-29	20.957115365539455	28.887394501742094	28.891139330326677	21.264350802391778
30-34	20.973923965983417	28.89908793650099	28.869321350315857	21.257666747199742
35-39	20.903734062812703	28.87137197353036	28.972894097435685	21.251999866221247
40-44	20.91590760380039	28.930898635985653	29.001010420176765	21.15218334003719
45-49	21.332638182913474	28.73790732436229	28.712170375541536	21.2172841171827
50-54	21.193254393561954	28.749374539576095	28.83601011543137	21.221360951430583
55-59	21.057438037612172	28.83995024146495	28.898589928006036	21.204021792916837
60-64	21.049972792624136	28.814680379043057	28.84993156858761	21.2854152597452
65-69	20.885278453882176	29.006856323816844	28.852053800866745	21.255811421434238
70-74	20.67186065657212	29.143333436135745	28.904898035608745	21.279907871683392
75-79	20.87903219047165	29.06536418457985	28.821130077296285	21.23447354765221
80-84	21.0589271806608	29.005139333744378	28.71583383018903	21.22009965540579
85-89	21.246458301105864	28.863179245544796	28.6112455363344	21.27911691701494
90-94	20.90583425086448	28.02529359056412	29.414167712150945	21.65470444642045
95-99	21.042418977149097	28.437904572048755	29.069703293253223	21.44997315754892
100-104	21.09692839560835	28.725412673616674	28.785022595816805	21.39263633495817
105-109	21.0655352297426	28.780591947428164	28.78914324839124	21.364729574437995
110-114	20.975136976142075	28.894913830067416	28.85275298034418	21.277196213446324
115-119	20.96268058536957	28.971997407396827	28.82441142005719	21.24091058717642
120-124	20.872986047530972	29.03417931282421	28.947735659076685	21.14509898056813
125-129	20.80036653677254	29.15871782995858	29.009134081827774	21.031781551441107
130-134	20.851328080770823	29.198871606546255	29.000948642222347	20.948851670460574
135-139	20.826652830808566	29.224080159100374	29.08413394872349	20.865133061367576
140-144	20.678732450738163	29.395069618287508	29.226385579587223	20.69981235138711
145-149	20.627655218194686	29.514027431155814	29.265569594359604	20.592747756289892
150-154	20.47479018165557	29.65854240181736	29.43110076261748	20.435566653909586
155-159	20.28112857418974	29.965181021634553	29.528691135213396	20.224999268962307
160-164	20.024043115867865	30.342252404657486	29.573077696440897	20.06062678303375
165-169	20.067659665688915	30.490451526988565	29.763389044249127	19.678499763073397
170-174	20.187873735374957	30.636905385385223	29.800327016064664	19.374893863175156
175-179	20.344691116340893	31.006177869644567	29.640545824966402	19.008585189048137
180	20.379901512942798	30.538696621612786	30.212095806817914	18.8693060586265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	2.0
3	3.0
4	5.0
5	10.0
6	22.5
7	36.5
8	68.0
9	144.5
10	281.0
11	492.5
12	802.0
13	1262.0
14	1916.0
15	2930.0
16	4380.0
17	6468.5
18	9442.5
19	13621.5
20	19325.0
21	26872.0
22	36738.5
23	48896.5
24	63761.5
25	81352.0
26	101529.0
27	124134.5
28	148577.0
29	174425.0
30	201733.0
31	230021.0
32	259321.5
33	289223.5
34	320277.5
35	352575.0
36	385095.5
37	417750.5
38	449396.0
39	479409.0
40	506206.5
41	529300.0
42	547223.5
43	557814.0
44	561209.0
45	556719.5
46	543125.0
47	522066.5
48	495105.0
49	461061.5
50	422053.5
51	379715.5
52	335333.5
53	291765.5
54	249407.5
55	209640.0
56	174493.5
57	144064.0
58	117487.0
59	94943.5
60	76304.0
61	61286.5
62	49079.0
63	38685.5
64	30239.0
65	23370.5
66	17684.0
67	13334.0
68	9972.0
69	7150.0
70	4983.0
71	3430.0
72	2279.5
73	1437.5
74	896.5
75	570.0
76	345.0
77	184.5
78	101.0
79	52.0
80	25.5
81	16.5
82	12.0
83	4.5
84	2.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	4342.0
92-93	10198.0
94-95	16169.0
96-97	22300.0
98-99	28694.0
100-101	34960.0
102-103	40933.0
104-105	47138.0
106-107	53592.0
108-109	59747.0
110-111	64517.0
112-113	70523.0
114-115	76326.0
116-117	82059.0
118-119	88513.0
120-121	96328.0
122-123	102178.0
124-125	109771.0
126-127	117294.0
128-129	124231.0
130-131	132160.0
132-133	140777.0
134-135	186898.0
136-137	250309.0
138-139	255208.0
140-141	258999.0
142-143	261523.0
144-145	264460.0
146-147	268998.0
148-149	275592.0
150-151	278847.0
152-153	285152.0
154-155	262840.0
156-157	266207.0
158-159	276000.0
160-161	290118.0
162-163	299991.0
164-165	308988.0
166-167	317260.0
168-169	321551.0
170-171	338982.0
172-173	350112.0
174-175	369939.0
176-177	393444.0
178-179	635860.0
180-181	3748919.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.86402868945132
#Duplication Level	Percentage of total
1	82.03410437598569
2	12.727467999251058
3	3.143261860984915
4	1.002222918183222
5	0.4153510782765819
6	0.21108686846033375
7	0.1341400558949578
8	0.08842588860162053
9	0.05265534110346174
>10	0.18875926213142652
>50	0.0025243511267381423
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.278470238336938E-4	8.137393708346207E-6	0.0	8.137393708346207E-6	0.0	0.0
2	0.0035479036568389463	1.6274787416692414E-5	8.137393708346207E-6	8.137393708346207E-6	0.0	0.0
3	0.0035479036568389463	4.068696854173104E-5	1.6274787416692414E-5	4.068696854173104E-5	8.137393708346207E-6	0.0
4	0.0035560410505472927	4.068696854173104E-5	1.6274787416692414E-5	5.696175595842345E-5	8.137393708346207E-6	0.0
5	0.0035641784442556387	4.068696854173104E-5	1.6274787416692414E-5	6.509914966676965E-5	1.6274787416692414E-5	0.0
6	0.0035804532316723314	4.8824362250077244E-5	1.6274787416692414E-5	6.509914966676965E-5	2.4412181125038622E-5	0.0
7	0.0036211402002140625	5.696175595842345E-5	2.4412181125038622E-5	6.509914966676965E-5	2.4412181125038622E-5	0.0
8	0.0036292775939224084	5.696175595842345E-5	3.254957483338483E-5	7.323654337511587E-5	3.254957483338483E-5	0.0
9	0.0036455523813391007	5.696175595842345E-5	3.254957483338483E-5	8.137393708346208E-5	3.254957483338483E-5	0.0
10-14	0.0036927492648475093	6.672662840843889E-5	3.5804532316723305E-5	8.788385205013905E-5	3.254957483338483E-5	0.0
15-19	0.0037464560633225937	8.462889456680056E-5	5.5334277216754214E-5	1.0741359695016993E-4	3.9059489800061797E-5	0.0
20-24	0.003834339915372733	1.1392351191684691E-4	8.300141582513133E-5	1.5949291668358566E-4	5.5334277216754214E-5	0.0
25-29	0.0040198724919230265	1.367082143002163E-4	9.927620324182372E-5	2.0506232145032442E-4	1.2857082059187006E-4	0.0
30-34	0.0060883979725846315	1.6437535290859338E-4	1.2531586310853162E-4	2.1319971515867064E-4	2.734164286004326E-4	0.0
35-39	0.01227444466966942	1.8716005529196278E-4	1.3833569304188553E-4	2.2784702383369382E-4	5.224206760758266E-4	0.0
40-44	0.01932631005732224	2.0668980019199367E-4	1.448456080085625E-4	2.5388668370040173E-4	0.0010139192560599374	8.137393708346207E-6
45-49	0.026099876580149627	2.343569388003708E-4	1.5298300171690872E-4	2.7667138608377105E-4	0.0015037903573023792	1.4647308675023171E-5
50-54	0.03271395018629343	2.473767687337247E-4	1.8227761906695504E-4	2.962011309838019E-4	0.0018927577765613275	1.6274787416692414E-5
55-59	0.03928408186641215	2.571416411837401E-4	2.034348427086552E-4	3.075934821754866E-4	0.0023451968667453768	2.115722364170014E-5
60-64	0.04640430136121508	2.734164286004326E-4	2.083172789336629E-4	3.222407908505098E-4	0.002857852670371188	2.4412181125038622E-5
65-69	0.05255454352598314	2.9620113098380194E-4	2.343569388003708E-4	3.4828045071721764E-4	0.0033021543668468913	2.4412181125038622E-5
70-74	0.09721093271864548	3.1573087588383287E-4	2.4412181125038623E-4	3.9059489800061795E-4	0.003694376743589178	2.76671386083771E-5
75-79	0.7647066913056099	3.238682695921791E-4	2.473767687337247E-4	4.2802690905901055E-4	0.004006852661989672	3.254957483338483E-5
80-84	0.9998008779759567	3.3200566330052525E-4	2.913186947587942E-4	4.84988665017434E-4	0.004236327164565035	3.254957483338483E-5
85-89	1.0012688638009426	3.499079294588869E-4	3.2875070581718676E-4	5.094008461424726E-4	0.004472311582107076	3.254957483338483E-5
90-94	1.0025920040179195	3.694376743589178E-4	3.515354082005562E-4	5.533427721675421E-4	0.004514626029390476	3.254957483338483E-5
95-99	1.0042959742604474	3.743201105839255E-4	3.645552381339101E-4	6.168144430926426E-4	0.004519508465615484	3.254957483338483E-5
100-104	1.0057688425216578	3.7757506806726396E-4	3.743201105839255E-4	6.477365391843581E-4	0.004542293167998853	3.254957483338483E-5
105-109	1.007578598882394	3.9059489800061795E-4	3.8408498303394096E-4	7.014433376594431E-4	0.004555312997932207	3.254957483338483E-5
110-114	1.0095576130322637	4.0035977045063345E-4	4.052422066756411E-4	7.453852636845126E-4	0.004566705349123892	3.254957483338483E-5
115-119	1.0115626668420004	4.1012464290064883E-4	4.198895153506643E-4	7.811897960012359E-4	0.00459111753024893	3.254957483338483E-5
120-124	1.0177112815280267	4.44301696475703E-4	4.4592917521737215E-4	8.088569346096132E-4	0.004617157190115638	3.254957483338483E-5
125-129	1.0417442601062565	4.589490051507261E-4	4.5569404766738765E-4	8.300141582513132E-4	0.004664354073624046	3.254957483338483E-5
130-134	1.0743849737491746	4.7196883508408003E-4	4.7034135634241086E-4	8.804659992430596E-4	0.004693648690974094	3.254957483338483E-5
135-139	1.1135225825288368	4.7847875005075697E-4	4.84988665017434E-4	9.09760616593106E-4	0.00471806087209913	3.254957483338483E-5
140-144	1.1538856828009756	4.898711012424417E-4	4.996359736924572E-4	9.29290361493137E-4	0.004747355489449177	3.254957483338483E-5
145-149	1.1883247604534384	4.963810162091186E-4	5.126558036258111E-4	9.683498512931988E-4	0.004766885234349208	3.254957483338483E-5
150-154	1.217376883470976	5.142832823674802E-4	5.289305910425035E-4	9.91134553676568E-4	0.004783160021765901	3.254957483338483E-5
155-159	1.2534076353327914	5.305580697841728E-4	5.38695463492519E-4	0.0010139192560599376	0.0048059447241492696	3.254957483338483E-5
160-164	1.3122458742803595	5.403229422341882E-4	5.452053784591959E-4	0.0010301940434766297	0.004843376735207662	3.254957483338483E-5
165-169	1.3533738895610827	5.452053784591959E-4	5.452053784591959E-4	0.0010399589159266454	0.004877553788782717	4.3941926025069524E-5
>>END_MODULE
