##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479141
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12216901
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.11373530816039	39.0	38.0	39.0	36.0	39.0
2	39.72732397520451	40.0	40.0	41.0	38.0	41.0
3	39.758397403727834	40.0	40.0	41.0	38.0	41.0
4	39.752449987112115	40.0	40.0	41.0	38.0	41.0
5	39.706791763312154	40.0	40.0	41.0	38.0	41.0
6	39.69231902591336	40.0	40.0	41.0	38.0	41.0
7	39.67441153857267	40.0	40.0	41.0	37.0	41.0
8	39.65469811042915	40.0	39.0	41.0	37.0	41.0
9	39.63937458443839	40.0	39.0	41.0	37.0	41.0
10-14	39.514030391176945	40.0	39.0	41.0	37.0	41.0
15-19	39.391056160641725	40.0	39.0	41.0	37.0	41.0
20-24	39.483822157517686	40.0	39.0	41.0	37.0	41.0
25-29	39.38473969789884	40.0	39.0	41.0	36.6	41.0
30-34	39.129902763393105	40.0	38.2	41.0	35.8	41.0
35-39	38.78012785730195	40.0	38.0	41.0	35.0	41.0
40-44	38.26281974454896	39.6	36.8	40.6	34.0	41.0
45-49	37.59151878205447	38.8	35.6	40.0	33.0	41.0
50-54	36.95669705435118	37.6	35.0	40.0	32.6	41.0
55-59	36.34428341524582	36.6	34.4	39.0	32.0	40.6
60-64	35.67544589253854	35.8	33.8	38.8	31.4	40.0
65-69	35.55599029573867	35.0	34.0	38.0	31.8	40.0
70-74	35.48110984937997	35.0	34.0	38.0	31.8	40.0
75-79	35.45238970177462	35.0	34.0	38.0	31.0	40.6
80-84	35.58814707592376	35.0	34.0	39.0	31.0	41.0
85-89	35.72664026662735	35.0	33.8	39.4	30.6	41.0
90-94	39.01463884108308	39.8	39.0	40.4	36.8	41.0
95-99	39.38012180621107	40.0	39.0	41.0	36.0	41.0
100-104	39.17346901879408	40.0	38.2	41.0	35.4	41.0
105-109	39.03934919100297	40.0	38.0	41.0	35.0	41.0
110-114	38.88389514324252	40.0	38.0	41.0	35.0	41.0
115-119	38.682800409985944	40.0	37.6	41.0	34.2	41.0
120-124	38.408611228749066	40.0	36.8	41.0	34.0	41.0
125-129	38.09257524315674	39.6	36.0	41.0	33.8	41.0
130-134	37.59180342240355	38.8	35.0	40.4	33.0	41.0
135-139	37.43158969695338	38.6	35.0	40.6	33.0	41.0
140-144	36.926775224400714	37.2	35.0	40.0	33.0	41.0
145-149	36.35780948746414	36.2	35.0	39.2	32.4	41.0
150-154	35.737710772206796	35.2	34.0	38.0	32.0	40.0
155-159	35.14089299547226	35.0	34.0	36.6	32.0	39.0
160-164	34.60639255914889	35.0	34.0	35.8	31.0	37.2
165-169	34.19129453335938	35.0	34.0	35.0	31.0	36.4
170-174	33.8966618752752	35.0	33.6	35.0	31.0	36.0
175-179	33.60712518862864	34.4	33.0	35.0	30.2	35.2
180	32.36443711819227	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	78.0
31	1375.0
32	12458.0
33	71727.0
34	284896.0
35	871204.0
36	2259416.0
37	4321626.0
38	3660081.0
39	726533.0
40	7506.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.081933953627026	28.399346118954387	28.64937679367296	21.86934313374562
2	20.708516832542063	28.003165450878253	30.240230317001014	21.048087399578666
3	20.86520141237127	27.61793682374933	29.68350975423309	21.83335200964631
4	20.754011184996916	27.867885644649164	29.872927676175813	21.505175494178104
5	20.99300796494954	27.998671676229513	29.62393654495522	21.384383813865725
6	21.632875636792015	27.882177321400903	28.87977073727617	21.60517630453091
7	21.17731002322111	28.49424743639979	29.138035906159836	21.190406634219265
8	20.98703263618163	28.38641321559371	29.439830935848626	21.186723212376034
9	21.544506254081945	28.246917937699585	28.61308281044432	21.595492997774148
10-14	21.123631925968787	28.66044179289003	28.88539573169988	21.330530549441303
15-19	21.202737093474035	28.703929089709412	28.6789358446958	21.414397972120753
20-24	21.130262085286606	28.730104303865605	28.777384706645325	21.362248904202467
25-29	20.989581564097147	28.86106059138893	28.858758862006002	21.290598982507923
30-34	21.024966969937793	28.845421600780753	28.847148716356134	21.282462712925316
35-39	20.93140314389058	28.840970390117754	28.95070198244219	21.276924483549468
40-44	20.967738054028594	28.883369031147915	28.96996546014411	21.178927454679382
45-49	21.378912704621243	28.71438673359144	28.674615600142783	21.232084961644528
50-54	21.238530131331995	28.692885372485215	28.826226880286583	21.242357615896207
55-59	21.100786525158878	28.80026939728823	28.859821324573225	21.239122752979664
60-64	21.08270665367592	28.795561165634393	28.81979808136286	21.301934099326825
65-69	20.929052302216412	28.985565160919286	28.809176729843355	21.276205807020947
70-74	20.710206295360827	29.121797745598492	28.859058446982587	21.30893751205809
75-79	20.934963785005706	29.013045124946167	28.796376429669028	21.255614660379095
80-84	21.098748365072286	28.99800694136754	28.65199120464347	21.251253488916706
85-89	21.291257087210578	28.857390266156695	28.557446769847772	21.293905876784955
90-94	20.919977745786543	27.9886844770146	29.41194621626534	21.67939156093352
95-99	21.08636871424318	28.401536474985335	29.03343821086748	21.47865659990401
100-104	21.142171799018982	28.70159451044232	28.738762316359807	21.417471374178895
105-109	21.09394542983022	28.743577047532288	28.7440274079332	21.418450114704292
110-114	21.006140609177642	28.874175934815447	28.818436819211673	21.301246636795234
115-119	21.007129569726168	28.95198567532416	28.780287203780247	21.260597551169422
120-124	20.902790316007465	29.010191965084402	28.900079417387314	21.186938301520815
125-129	20.833857965128047	29.124758410527235	28.971249342177526	21.070134282167192
130-134	20.88701524624139	29.169447745652406	28.954970492392935	20.988566515713273
135-139	20.870605071723254	29.19471381585025	29.032846425848263	20.901834686578233
140-144	20.714788567032098	29.368031774935723	29.177338772529954	20.739840885502222
145-149	20.681563189534792	29.469596952243897	29.22178185364897	20.627058004572334
150-154	20.5271817311316	29.6208290143823	29.37654032994807	20.47544892453803
155-159	20.326938146323002	29.923384758089068	29.489170876078745	20.260506219509185
160-164	20.067081486849894	30.306311394246404	29.537751972293076	20.088855146610626
165-169	20.092002115113058	30.447347295705736	29.729424742782456	19.731225846398754
170-174	20.246704890431335	30.610469169981787	29.71492807126322	19.427897868323658
175-179	20.384308461893237	30.956430825675653	29.599988193820252	19.059272518610854
180	20.382785708682423	30.560982996941643	30.13191929508662	18.92431199928931
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	3.0
3	4.0
4	4.0
5	8.0
6	19.0
7	33.0
8	67.0
9	131.5
10	269.0
11	503.5
12	802.5
13	1240.5
14	1871.0
15	2818.5
16	4289.0
17	6398.5
18	9207.5
19	13161.0
20	18840.5
21	26396.0
22	35884.5
23	47789.5
24	62677.5
25	80101.5
26	100036.5
27	122006.0
28	145817.5
29	171937.5
30	199112.0
31	227233.0
32	256685.0
33	286662.0
34	317415.5
35	348657.5
36	380731.0
37	413473.0
38	445115.5
39	475010.5
40	501835.5
41	524652.5
42	542443.5
43	552979.5
44	556735.0
45	552594.5
46	540172.0
47	519927.0
48	492235.0
49	459406.0
50	421058.0
51	378748.0
52	335139.0
53	291658.0
54	249785.5
55	211394.5
56	176227.0
57	144795.5
58	118775.0
59	96498.0
60	77741.0
61	62626.0
62	50210.5
63	39733.5
64	30967.0
65	24276.0
66	18716.5
67	13943.5
68	10231.5
69	7334.5
70	5151.0
71	3578.0
72	2389.0
73	1556.0
74	972.0
75	587.5
76	357.0
77	197.0
78	99.5
79	57.0
80	29.5
81	18.0
82	12.5
83	5.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	4176.0
92-93	9977.0
94-95	15954.0
96-97	21893.0
98-99	27766.0
100-101	33572.0
102-103	40198.0
104-105	45757.0
106-107	52702.0
108-109	58608.0
110-111	63751.0
112-113	69135.0
114-115	74741.0
116-117	80257.0
118-119	87454.0
120-121	94436.0
122-123	100174.0
124-125	106986.0
126-127	114335.0
128-129	121348.0
130-131	129104.0
132-133	137183.0
134-135	184188.0
136-137	246715.0
138-139	248832.0
140-141	256099.0
142-143	259118.0
144-145	259401.0
146-147	265542.0
148-149	272690.0
150-151	275815.0
152-153	286512.0
154-155	261444.0
156-157	261576.0
158-159	273184.0
160-161	282274.0
162-163	294407.0
164-165	304660.0
166-167	314857.0
168-169	321055.0
170-171	332598.0
172-173	345058.0
174-175	367525.0
176-177	384973.0
178-179	579093.0
180-181	3849778.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.96391093107803
#Duplication Level	Percentage of total
1	82.20881029961147
2	12.683502629886728
3	2.9440000507947603
4	1.0367004014016714
5	0.45501451443466684
6	0.21724115670358302
7	0.11470787231951263
8	0.06890649103149345
9	0.043375269351249454
>10	0.22244525865272807
>50	0.0037431014415967904
>100	0.0015529543705419765
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1459534623387715E-4	0.0	0.0	8.185381873848368E-6	0.0	0.0
2	0.0031104451120623797	8.185381873848368E-6	8.185381873848368E-6	1.6370763747696736E-5	0.0	0.0
3	0.0031104451120623797	8.185381873848368E-6	1.6370763747696736E-5	4.092690936924184E-5	0.0	0.0
4	0.0031104451120623797	8.185381873848368E-6	1.6370763747696736E-5	5.7297673116938575E-5	0.0	0.0
5	0.003118630493936228	8.185381873848368E-6	1.6370763747696736E-5	6.548305499078694E-5	0.0	0.0
6	0.0031268158758100766	8.185381873848368E-6	1.6370763747696736E-5	6.548305499078694E-5	1.6370763747696736E-5	0.0
7	0.003143186639557773	8.185381873848368E-6	1.6370763747696736E-5	7.366843686463531E-5	1.6370763747696736E-5	0.0
8	0.0031513720214316216	8.185381873848368E-6	1.6370763747696736E-5	7.366843686463531E-5	2.4556145621545103E-5	0.0
9	0.00315955740330547	1.6370763747696736E-5	1.6370763747696736E-5	9.003920061233204E-5	2.4556145621545103E-5	0.0
10-14	0.003223403381921487	2.783029837108445E-5	2.1281992872005757E-5	9.658750611141074E-5	2.4556145621545103E-5	0.0
15-19	0.0033199908880328977	4.747521486832053E-5	2.619322199631478E-5	1.0804704073479846E-4	3.274152749539347E-5	0.0
20-24	0.003383836866648915	7.039428411509597E-5	3.928983299447217E-5	1.3424026273111325E-4	5.7297673116938575E-5	0.0
25-29	0.0036081163299923604	9.167627698710172E-5	4.092690936924184E-5	1.7025594297604606E-4	1.244178044824952E-4	0.0
30-34	0.005883652490922207	1.0640996436002878E-4	5.7297673116938575E-5	1.9317501222282143E-4	3.1922989308008637E-4	0.0
35-39	0.012256790817900546	1.3260318635634357E-4	6.057182586647793E-5	2.1609408146959692E-4	6.27000251536785E-4	6.548305499078695E-6
40-44	0.018929514121461738	1.4733687372927063E-4	8.185381873848367E-5	2.668434490874568E-4	0.001113211934843378	1.6370763747696736E-5
45-49	0.025594052043149074	1.686188666012764E-4	9.003920061233204E-5	3.06133282081929E-4	0.0015584967087807293	2.1281992872005757E-5
50-54	0.03217509906972317	1.7189301935081574E-4	9.33133533618714E-5	3.536084969502495E-4	0.0020463454684620922	2.4556145621545103E-5
55-59	0.039077013065752106	1.8007840122466408E-4	9.822458248618041E-5	4.3055108656442413E-4	0.0024261471874086563	2.619322199631478E-5
60-64	0.04626050419824143	1.931750122228215E-4	1.1295826985910748E-4	4.649296904345872E-4	0.0028501499684740013	3.6015680244932824E-5
65-69	0.0525354179427336	1.9972331772190013E-4	1.2605488085726488E-4	4.829375305570537E-4	0.003367466102901219	4.092690936924184E-5
70-74	0.09788734475297786	2.0627162322097887E-4	1.4569979735450095E-4	5.107678289281382E-4	0.0038258474878367275	4.092690936924184E-5
75-79	0.7725870906214267	2.1773115784436657E-4	1.5388517922834932E-4	5.46783509173071E-4	0.004023933729183858	4.092690936924184E-5
80-84	1.0100450187817678	2.5374683808929946E-4	1.784413248498944E-4	6.040811822900096E-4	0.004192552595785134	4.092690936924184E-5
85-89	1.0114774606096915	2.815771364603839E-4	1.9808624134713047E-4	6.597417790321784E-4	0.0044643072739969005	4.092690936924184E-5
90-94	1.0127281869600155	3.1922989308008637E-4	2.1118285234528788E-4	6.908462301528024E-4	0.004485589266868905	4.092690936924184E-5
95-99	1.014352166723787	3.257781985791651E-4	2.341019215920633E-4	7.317731395220441E-4	0.0044970488014922936	4.092690936924184E-5
100-104	1.015882833134197	3.3396358045301345E-4	2.3737607434160267E-4	7.596034378931285E-4	0.004516693717989529	4.092690936924184E-5
105-109	1.0176868912991928	3.5688264969978886E-4	2.471985325902207E-4	7.956191181380613E-4	0.004541249863611074	4.092690936924184E-5
110-114	1.019698858163785	3.732534134474856E-4	2.913995947090019E-4	8.299977220082244E-4	0.004582176772980316	4.092690936924184E-5
115-119	1.0218990888114754	3.9617248269426095E-4	3.11044511206238E-4	8.578280203793089E-4	0.004595273383978474	4.092690936924184E-5
120-124	1.0282935091313257	4.0108371181857007E-4	3.306894277034741E-4	8.741987841270057E-4	0.004605095842227092	4.092690936924184E-5
125-129	1.0532392789300657	4.1745447556626675E-4	3.699792606979462E-4	8.905695478747023E-4	0.004659119362594491	4.092690936924184E-5
130-134	1.0859513390507134	4.256398574401151E-4	3.8471294807087327E-4	9.151256934962475E-4	0.004706594577462812	4.092690936924184E-5
135-139	1.1254310729046588	4.3382523931396344E-4	4.0435786456810937E-4	9.396818391177927E-4	0.00471641703571143	4.092690936924184E-5
140-144	1.1668343714989589	4.3382523931396344E-4	4.092690936924184E-4	9.838829012365738E-4	0.0047442473340825134	4.9112291243090205E-5
145-149	1.2019971349526366	4.3382523931396344E-4	4.1745447556626675E-4	0.0010018907413590402	0.004778625937952677	4.9112291243090205E-5
150-154	1.231176384256531	4.4201062118781186E-4	4.1909155194103646E-4	0.0010329951924796638	0.0048080933126985305	4.9112291243090205E-5
155-159	1.2681137385004593	4.4201062118781186E-4	4.3055108656442413E-4	0.001062462567225518	0.004832649458320076	4.9112291243090205E-5
160-164	1.3269076994239375	4.4201062118781186E-4	4.3382523931396344E-4	0.001093567018346142	0.0048555685275668515	4.9112291243090205E-5
165-169	1.3678477054041773	4.4201062118781186E-4	4.4201062118781186E-4	0.0011050265529695296	0.004893221284186554	4.9112291243090205E-5
>>END_MODULE
