##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479142
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10553620
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.246261567121046	39.0	39.0	39.0	37.0	39.0
2	39.94420331601858	41.0	40.0	41.0	38.0	41.0
3	39.97720516751598	41.0	40.0	41.0	38.0	41.0
4	39.976463810521885	41.0	40.0	41.0	38.0	41.0
5	39.977515108559906	41.0	40.0	41.0	38.0	41.0
6	39.95888984064236	41.0	40.0	41.0	38.0	41.0
7	39.978532863605096	41.0	40.0	41.0	38.0	41.0
8	39.948769711246	41.0	40.0	41.0	38.0	41.0
9	39.92239677001825	41.0	40.0	41.0	38.0	41.0
10-14	39.8328171755284	41.0	40.0	41.0	38.0	41.0
15-19	39.77134090482697	40.6	39.8	41.0	37.8	41.0
20-24	39.76175124743927	41.0	40.0	41.0	37.6	41.0
25-29	39.59531671597044	40.4	39.2	41.0	36.8	41.0
30-34	39.33475368641281	40.0	38.8	41.0	35.6	41.0
35-39	38.97042281226726	40.0	38.0	41.0	35.0	41.0
40-44	38.52820361165173	40.0	37.0	41.0	35.0	41.0
45-49	38.136343245256136	39.2	35.8	41.0	34.4	41.0
50-54	37.53256232458626	38.2	35.0	40.2	34.0	41.0
55-59	36.833266481074745	36.6	35.0	39.4	33.8	41.0
60-64	35.86260407329428	35.4	34.6	38.2	32.4	40.0
65-69	35.47055412266123	35.0	34.6	37.0	32.8	39.0
70-74	35.28789639952926	35.0	35.0	36.0	33.0	38.8
75-79	35.04922523266898	35.0	34.2	35.8	33.0	38.0
80-84	34.95737695691147	35.0	34.0	35.0	33.0	38.0
85-89	34.6489661746396	34.8	33.4	35.0	31.8	38.6
90-94	39.50793597818299	40.6	39.8	40.6	37.2	40.8
95-99	39.6929041942645	41.0	39.6	41.0	37.0	41.0
100-104	39.53016468065125	40.6	39.0	41.0	36.4	41.0
105-109	39.49926233500854	41.0	39.0	41.0	36.0	41.0
110-114	39.35636983796087	40.2	39.0	41.0	35.4	41.0
115-119	39.137315707733976	40.0	38.0	41.0	35.0	41.0
120-124	38.84697036758111	40.0	38.0	41.0	35.0	41.0
125-129	38.22863861923684	39.4	36.4	40.4	34.4	40.8
130-134	38.31858018082454	39.6	36.0	40.8	34.4	41.0
135-139	37.909207586993844	39.0	35.0	41.0	34.0	41.0
140-144	37.288412209827655	37.4	35.0	40.2	34.0	41.0
145-149	36.59874927473626	36.2	35.0	39.0	34.0	41.0
150-154	35.899612411155275	35.2	35.0	37.2	33.0	39.6
155-159	35.307114359833164	35.0	35.0	36.2	33.0	38.4
160-164	34.855433070206814	35.0	34.6	35.6	33.0	36.8
165-169	34.566758340336165	35.0	34.0	35.0	33.0	36.0
170-174	34.38105519971258	35.0	34.0	35.0	33.0	36.0
175-178	33.86808268003133	34.75	33.25	35.0	31.75	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	5.0
30	222.0
31	2594.0
32	19227.0
33	85812.0
34	272345.0
35	698439.0
36	1668082.0
37	3208095.0
38	3634687.0
39	954902.0
40	9210.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.590603034788064	28.461760040630608	29.41016447436993	21.537472450211396
2	21.30254831991298	28.815543860779524	29.719328533716393	20.162579285591107
3	20.137583123136896	27.86099935377624	30.079792526166376	21.92162499692049
4	20.63299607149016	27.145519736355865	30.62213723821779	21.599346953936184
5	21.21630303156642	27.89673116902068	29.181048777575846	21.705917021837056
6	21.79685264392692	27.691123993473333	28.520545556880013	21.991477805719743
7	21.623916722413732	28.32286930929861	28.366863692268623	21.686350276019034
8	21.389182100549384	28.484046232477578	28.591668072187552	21.535103594785486
9	22.013953506000785	28.211580481389326	27.89721441552756	21.877251597082328
10-14	21.64794260168549	28.466588715530783	28.15096810383546	21.734500578948264
15-19	21.699574174548637	28.38991739327359	28.160790325973455	21.749718106204316
20-24	21.57043933740271	28.349235617731168	28.351291784240857	21.729033260625265
25-29	21.547637682615065	28.448274620461984	28.210776965628853	21.793310731294095
30-34	21.64458261714938	28.44387423462281	28.144159065799222	21.76738408242859
35-39	21.51932701764892	28.431061569395144	28.225774663101383	21.823836749854554
40-44	21.537313263126777	28.48000022741012	28.202131590866454	21.780554918596653
45-49	21.847591632065587	28.291251722157895	28.004769927285615	21.856386718490906
50-54	21.72876226356454	28.345538308182405	28.132847307369413	21.79285212088364
55-59	21.66610319492269	28.350382143757308	28.153498041430336	21.83001661988967
60-64	21.715803676842636	28.350249487853457	28.097716233860986	21.836230601442917
65-69	21.646974213587374	28.342348881236962	28.156082936471087	21.854593968704577
70-74	21.593625694311523	28.408231488342388	28.179877615453275	21.81826520189281
75-79	21.7274110684296	28.314123495066145	28.10187973415757	21.856585702346685
80-84	21.729366795469232	28.339885271594014	28.095665752604322	21.835082180332435
85-89	21.75739509286861	28.38775699712516	28.011726781900432	21.843121128105807
90-94	20.83471806560354	27.99180923359632	29.61691472565015	21.55655797514999
95-99	21.559250785383583	28.246191429379525	28.382262355491278	21.81229542974561
100-104	21.62644490918262	28.293390859730444	28.241142978660672	21.839021252426264
105-109	21.479695071410614	28.32605882922165	28.43912516417167	21.75512093519607
110-114	21.575185862744483	28.193224748962837	28.392884065881415	21.83870532241127
115-119	21.559798640911286	28.274029511499027	28.322664892978043	21.843506954611648
120-124	21.496726343305937	28.34437561563282	28.362751618995258	21.79614642206598
125-129	21.58686913550713	28.298600917126173	28.319165600745794	21.7953643466209
130-134	21.627475772370463	28.29263744497059	28.333665999049995	21.746220783608948
135-139	21.619809758351977	28.352280156012018	28.31574923692489	21.712160848711115
140-144	21.659163759074232	28.39492596662706	28.32622328255207	21.619686991746637
145-149	21.559295966195467	28.452080152342358	28.402392892356165	21.586230989106014
150-154	21.422317694462087	28.587309577515573	28.50558113773155	21.484791590290783
155-159	21.454333996238216	28.616517756950238	28.474959220431835	21.454189026379712
160-164	21.375288197792624	28.733396413398417	28.592873659717284	21.298441729091675
165-169	21.28925719634841	28.849625756740664	28.678962592946323	21.182154453964603
170-174	21.39776699579453	28.967516123493258	28.603867710930793	21.03084916978142
175-178	21.35606359132047	29.09607225457898	28.64610309626815	20.901761057832402
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.5
6	4.0
7	6.5
8	14.0
9	33.0
10	84.0
11	199.0
12	388.5
13	693.5
14	1255.5
15	2168.5
16	3638.5
17	5882.5
18	9041.0
19	13525.5
20	19434.5
21	27067.5
22	36592.0
23	48261.0
24	61427.5
25	76694.0
26	94169.0
27	112446.0
28	131759.0
29	152451.5
30	174868.5
31	197759.0
32	221080.5
33	245019.0
34	268431.0
35	293533.0
36	319066.0
37	343434.5
38	367609.0
39	389996.0
40	409527.0
41	424700.5
42	434928.5
43	438939.0
44	436430.0
45	427853.0
46	412673.5
47	392445.0
48	367622.0
49	339889.0
50	310474.0
51	280272.5
52	251633.0
53	225433.5
54	202540.5
55	182466.0
56	164868.5
57	150093.5
58	137476.0
59	126484.5
60	116855.0
61	107407.5
62	97656.5
63	87836.0
64	78203.0
65	68284.0
66	58030.0
67	48638.0
68	39894.5
69	31870.0
70	24834.0
71	18671.5
72	13665.5
73	9710.0
74	6669.0
75	4466.5
76	2883.0
77	1784.5
78	1100.0
79	639.5
80	345.0
81	167.0
82	83.5
83	48.0
84	22.0
85	9.5
86	5.5
87	2.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1256.0
92-93	2802.0
94-95	4505.0
96-97	6065.0
98-99	7456.0
100-101	9100.0
102-103	10395.0
104-105	11894.0
106-107	13492.0
108-109	15229.0
110-111	16755.0
112-113	17892.0
114-115	18977.0
116-117	20694.0
118-119	21795.0
120-121	23469.0
122-123	24727.0
124-125	26346.0
126-127	28152.0
128-129	29788.0
130-131	31475.0
132-133	35729.0
134-135	108148.0
136-137	111035.0
138-139	109520.0
140-141	111630.0
142-143	116981.0
144-145	116520.0
146-147	116537.0
148-149	117562.0
150-151	124841.0
152-153	127287.0
154-155	123367.0
156-157	125003.0
158-159	130295.0
160-161	135793.0
162-163	146799.0
164-165	147372.0
166-167	153822.0
168-169	162035.0
170-171	170799.0
172-173	174717.0
174-175	187572.0
176-177	766145.0
178-179	6591847.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.46724959004254
#Duplication Level	Percentage of total
1	87.86866780844184
2	9.851210996429893
3	1.570659887740622
4	0.39022262018612613
5	0.14460887227119873
6	0.0746931975314209
7	0.032897013955572936
8	0.01446037510827344
9	0.013490766058415997
>10	0.039088462276645954
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.47542170364292E-6	9.47542170364292E-6	1.895084340728584E-5	0.0	0.0	0.0
2	3.790168681457168E-5	9.47542170364292E-6	4.73771085182146E-5	0.0	0.0	0.0
3	3.790168681457168E-5	3.790168681457168E-5	6.632795192550044E-5	1.895084340728584E-5	0.0	0.0
4	4.73771085182146E-5	4.73771085182146E-5	7.580337362914336E-5	2.8426265110928763E-5	9.47542170364292E-6	0.0
5	4.73771085182146E-5	4.73771085182146E-5	7.580337362914336E-5	3.790168681457168E-5	9.47542170364292E-6	0.0
6	4.73771085182146E-5	4.73771085182146E-5	8.527879533278629E-5	4.73771085182146E-5	9.47542170364292E-6	0.0
7	4.73771085182146E-5	4.73771085182146E-5	8.527879533278629E-5	5.6852530221857526E-5	1.895084340728584E-5	0.0
8	4.73771085182146E-5	5.6852530221857526E-5	9.47542170364292E-5	7.580337362914336E-5	3.790168681457168E-5	0.0
9	5.6852530221857526E-5	5.6852530221857526E-5	9.47542170364292E-5	8.527879533278629E-5	3.790168681457168E-5	0.0
10-14	7.201320494768619E-5	8.338371099205771E-5	1.0043947005861495E-4	9.285913269570063E-5	6.632795192550045E-5	0.0
15-19	1.1560014478444362E-4	1.3834115687318664E-4	1.1560014478444362E-4	1.288657351695437E-4	9.854438571788638E-5	0.0
20-24	1.2507556648808655E-4	1.8571826539140126E-4	1.2318048214735798E-4	1.8192809670994407E-4	1.3076081951027228E-4	0.0
25-29	1.402362412139152E-4	2.577314703390874E-4	1.2697065082881514E-4	2.1224944616160145E-4	1.9708877143577276E-4	0.0
30-34	5.040924346338034E-4	3.032134945165735E-4	1.5918708462120106E-4	2.6152163902054456E-4	2.9184298847220193E-4	0.0
35-39	0.0021300747989789286	3.354299283089594E-4	1.7055759066557258E-4	3.2974467528677364E-4	3.82807036827174E-4	0.0
40-44	0.0034111518133114515	3.733316151235311E-4	1.9708877143577276E-4	3.7712178380498826E-4	4.946170129301605E-4	0.0
45-49	0.004263939766639314	4.074431332566456E-4	2.0466910879868712E-4	4.207087236417457E-4	6.253778324404328E-4	1.3265590385100087E-5
50-54	0.005006812828204919	4.5292515743413167E-4	2.2741012088743008E-4	4.51030073093403E-4	7.769845796987195E-4	1.895084340728584E-5
55-59	0.005598079142512238	4.83246506885789E-4	2.6341672336127316E-4	4.870366755672461E-4	8.887945558017059E-4	2.842626511092876E-5
60-64	0.006174184782093727	5.249383623818179E-4	2.899479041314734E-4	5.476793744705608E-4	0.0010460865560821785	3.790168681457168E-5
65-69	0.006755975674697402	5.96951567329504E-4	3.0510857885730205E-4	5.988466516702325E-4	0.0011787424599331794	3.790168681457168E-5
70-74	0.013887178048859066	6.519090132106329E-4	3.221643379238593E-4	6.500139288699045E-4	0.0013909919060947806	3.790168681457168E-5
75-79	0.1538656877924352	6.879156156844762E-4	3.3732501264968796E-4	6.727549409586474E-4	0.001489536291812667	3.790168681457168E-5
80-84	0.21761063976152256	7.106566277732191E-4	3.524856873755166E-4	7.106566277732191E-4	0.0017245267500630116	4.73771085182146E-5
85-89	0.2180351386538458	7.258173024990479E-4	3.695414464420739E-4	7.807747483801767E-4	0.0021660814014527715	4.73771085182146E-5
90-94	0.2182682340277554	7.561386519507051E-4	3.941775428715455E-4	8.205715195354769E-4	0.0022020880039266147	4.73771085182146E-5
95-99	0.21849753923298354	7.807747483801767E-4	4.1691855496028845E-4	8.603682906907772E-4	0.002215353594311715	4.73771085182146E-5
100-104	0.21879506747447794	8.01620676128191E-4	4.3207922968611717E-4	8.982699775053488E-4	0.0022627307028299294	4.73771085182146E-5
105-109	0.21908691046295017	8.205715195354769E-4	4.3965956704903155E-4	9.266962426162777E-4	0.0022987373053037726	4.73771085182146E-5
110-114	0.2193635927766965	8.4331253162422E-4	4.7377108518214606E-4	9.627028450901208E-4	0.0023612750885478157	4.73771085182146E-5
115-119	0.21963269475307995	8.584732063500486E-4	4.8324650688578893E-4	0.0010081848692676068	0.0024617145586064305	4.73771085182146E-5
120-124	0.220714787911636	8.812142184387916E-4	5.05987518974532E-4	0.0010422963874007214	0.0025602589443243172	5.116727719967178E-5
125-129	0.2230855384218875	9.266962426162777E-4	5.249383623818178E-4	0.0010631423151487359	0.0027346067036713466	5.685253022185753E-5
130-134	0.2258523615593512	9.570175920679349E-4	5.419941214483751E-4	0.0010953587489411216	0.002817990414663405	5.685253022185753E-5
135-139	0.22885986040808745	9.854438571788638E-4	5.742105552407609E-4	0.0011048341706447642	0.0029051642943369194	5.685253022185753E-5
140-144	0.23212130055848137	0.0010138701222897925	5.912663143073183E-4	0.0011370506044371506	0.0030359251138471917	6.632795192550044E-5
145-149	0.23423242451405302	0.0010460865560821785	5.96951567329504E-4	0.0011541063635037078	0.0031325744152243496	6.632795192550044E-5
150-154	0.23639471574682433	0.0010820931585560213	6.121122420553328E-4	0.0011920080503182793	0.0031780564394018355	6.632795192550044E-5
155-159	0.23940789984858277	0.0011029390863040362	6.424335915069901E-4	0.0012412802431772227	0.0032462794756680643	6.632795192550044E-5
160-164	0.2423812871791859	0.0011275751827335076	6.689647722771901E-4	0.001265916339606694	0.0033069221745713794	6.632795192550044E-5
165-167	0.2451796950556618	0.001133892130535936	6.759134148598616E-4	0.0012697065082881514	0.003335348439682308	6.632795192550044E-5
>>END_MODULE
