##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479144
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10781055
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.206616699386096	39.0	39.0	39.0	37.0	39.0
2	39.900385722918585	41.0	40.0	41.0	38.0	41.0
3	39.93189655372318	41.0	40.0	41.0	38.0	41.0
4	39.928940071263895	41.0	40.0	41.0	38.0	41.0
5	39.92371850435788	41.0	40.0	41.0	38.0	41.0
6	39.90522096399657	41.0	40.0	41.0	38.0	41.0
7	39.92551090779149	41.0	40.0	41.0	38.0	41.0
8	39.90031995941028	41.0	40.0	41.0	38.0	41.0
9	39.8745972448893	41.0	40.0	41.0	38.0	41.0
10-14	39.79322363163902	41.0	40.0	41.0	38.0	41.0
15-19	39.722972677534806	40.6	39.6	41.0	37.6	41.0
20-24	39.68937629944379	41.0	39.6	41.0	37.0	41.0
25-29	39.53128232812095	40.0	39.0	41.0	36.6	41.0
30-34	39.28212884546086	40.0	38.6	41.0	35.6	41.0
35-39	38.93610398982288	40.0	38.0	41.0	35.0	41.0
40-44	38.52493736466422	40.0	37.0	41.0	34.8	41.0
45-49	38.15890238942293	39.2	35.8	41.0	34.4	41.0
50-54	37.57609376818873	38.2	35.0	40.4	34.0	41.0
55-59	36.88484305107431	36.8	35.0	39.4	33.8	41.0
60-64	35.944499179347474	35.6	34.6	38.2	32.6	40.2
65-69	35.536455810679016	35.0	34.6	37.0	32.8	39.0
70-74	35.328071621933105	35.0	35.0	36.2	33.0	39.0
75-79	35.086423452992314	35.0	34.4	36.0	33.0	38.0
80-84	34.97781272797514	35.0	34.0	35.0	33.0	38.0
85-89	34.697428999295525	34.8	33.6	35.0	32.2	38.4
90-94	39.41544330512016	40.6	39.4	40.6	36.8	40.8
95-99	39.633426118052135	41.0	39.0	41.0	37.0	41.0
100-104	39.47504318076561	40.4	39.0	41.0	36.2	41.0
105-109	39.431132767504224	40.0	39.0	41.0	36.0	41.0
110-114	39.28172408845864	40.0	38.6	41.0	35.2	41.0
115-119	39.05786071209421	40.0	38.0	41.0	35.0	41.0
120-124	38.77108377536321	40.0	37.8	41.0	35.0	41.0
125-129	38.17160432197228	39.4	36.4	40.4	34.0	40.8
130-134	38.23432200503608	39.4	36.0	40.8	34.0	41.0
135-139	37.82590665488693	38.8	35.0	41.0	34.0	41.0
140-144	37.20254319073185	37.2	35.0	40.0	34.0	41.0
145-149	36.51063269835701	36.2	35.0	39.0	33.6	41.0
150-154	35.81574898022064	35.0	35.0	37.2	33.0	39.4
155-159	35.22813170731401	35.0	35.0	36.2	33.0	38.0
160-164	34.79742464401502	35.0	34.2	35.4	33.0	36.8
165-169	34.5126792867244	35.0	34.0	35.0	33.0	36.0
170-174	34.32850117963938	35.0	34.0	35.0	32.6	36.0
175-178	33.851347990781136	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	226.0
31	3008.0
32	21253.0
33	97027.0
34	305254.0
35	764199.0
36	1747614.0
37	3234209.0
38	3625466.0
39	972995.0
40	9798.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.49589766493168	28.507775908758465	29.472152771690713	21.524173654619144
2	21.241937825194288	28.866497759263822	29.772754150683767	20.118810264858126
3	20.105017551621803	27.907834622863902	30.110763742509427	21.87638408300486
4	20.555233230885104	27.182052220306822	30.671849832878134	21.590864715929932
5	21.1507779155194	27.94795129048131	29.243260515784403	21.65801027821489
6	21.752936053104264	27.739288965690278	28.566202472763564	21.941572508441894
7	21.581774696446683	28.35761435221321	28.41677368309502	21.643837268245083
8	21.346120579108447	28.55998786760665	28.614769148288367	21.47912240499654
9	21.9852046019615	28.243998384202655	27.945790091971517	21.825006921864325
10-14	21.60784635640946	28.51722025349096	28.194606186500298	21.680327203599276
15-19	21.661627734948016	28.44165993031294	28.20127529263138	21.69543704210766
20-24	21.52188445379418	28.39440295963614	28.40336868701625	21.68034389955343
25-29	21.50494177054101	28.493411822868914	28.26383688794835	21.73780951864173
30-34	21.596268639757426	28.49463990305216	28.188797849561105	21.72029360762931
35-39	21.470405261822705	28.480962206388895	28.275739248153357	21.772893283635042
40-44	21.497973992341198	28.524091566177894	28.249769619021514	21.728164822459398
45-49	21.794303062177125	28.346537514185766	28.04863902465946	21.81052039897765
50-54	21.682412342762373	28.39745275392807	28.184054343475662	21.736080559833894
55-59	21.61928122989819	28.42765202477865	28.186877814833522	21.76618893048964
60-64	21.658817249332277	28.3989516795898	28.14729356264299	21.794937508434938
65-69	21.604232609888363	28.380500795144815	28.21484724825168	21.80041934671514
70-74	21.547327232817196	28.46127396623058	28.231185166943305	21.760213634008917
75-79	21.695162486417146	28.38627202996367	28.124392278863247	21.794173204755936
80-84	21.68668094170747	28.413052340424937	28.142104831113468	21.758161886754127
85-89	21.735095498538872	28.450417885819153	28.044333323594028	21.77015329204795
90-94	20.772832942802538	28.03579099839057	29.67637101691569	21.515005041891197
95-99	21.49381312081816	28.289573900085863	28.43020205627188	21.7864109228241
100-104	21.56944566691757	28.36195924378318	28.277687856077417	21.790907233221834
105-109	21.422478338995884	28.397829256764734	28.474573062757948	21.70511934148143
110-114	21.5200013974291	28.2416466033008	28.44101265479254	21.797339344477557
115-119	21.496956400357913	28.32494220979963	28.377807644020503	21.800293745821953
120-124	21.43945043947565	28.394351851853607	28.42366152930681	21.74253617936394
125-129	21.549160240833164	28.335653432033546	28.364183314802478	21.75100301233081
130-134	21.562459485710175	28.35332267280491	28.390471640472704	21.693746201012214
135-139	21.566850755646684	28.41233092289587	28.3724370722934	21.648381249164053
140-144	21.599919123296534	28.438691414937928	28.370894496340327	21.590494965425208
145-149	21.50869293800681	28.488448651733066	28.446663055962624	21.5561953542975
150-154	21.375865196143064	28.642738109690924	28.55679951151918	21.424597182646828
155-159	21.39230740385529	28.67399304454061	28.51996393584756	21.41373561575654
160-164	21.3108412820592	28.772656147401165	28.654978483658777	21.261524086880854
165-169	21.24099497428081	28.886075849606137	28.723231086077057	21.149698090036004
170-174	21.335409531784652	29.019014683204702	28.663509008076048	20.982066776934598
175-178	21.28975671286377	29.146124380139	28.69945921902358	20.864659687973646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	3.0
7	8.0
8	19.0
9	38.5
10	87.5
11	199.5
12	373.5
13	711.5
14	1323.5
15	2322.5
16	3835.5
17	6137.0
18	9602.5
19	14229.0
20	20376.0
21	28428.0
22	38308.5
23	50228.0
24	64192.0
25	80109.0
26	97697.5
27	116689.5
28	137400.5
29	159445.5
30	181925.5
31	204375.0
32	227982.5
33	252720.0
34	277143.5
35	303308.0
36	329070.5
37	352986.5
38	376888.5
39	399174.0
40	418930.0
41	435171.0
42	444730.0
43	447860.5
44	445558.0
45	436625.5
46	420517.5
47	398975.5
48	373426.5
49	344382.0
50	313809.0
51	283164.5
52	254472.0
53	228042.0
54	204401.5
55	183882.0
56	166721.0
57	152228.5
58	139353.0
59	127859.5
60	117803.0
61	108299.0
62	98163.0
63	88429.0
64	78676.0
65	68535.0
66	58604.5
67	48836.5
68	39888.5
69	31835.5
70	24662.5
71	18821.0
72	13887.5
73	9811.0
74	6788.5
75	4555.5
76	2916.0
77	1785.5
78	1076.5
79	633.0
80	337.5
81	166.5
82	93.0
83	54.5
84	24.5
85	10.0
86	5.5
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1518.0
92-93	3317.0
94-95	5105.0
96-97	6761.0
98-99	8467.0
100-101	9929.0
102-103	11659.0
104-105	13404.0
106-107	14798.0
108-109	16492.0
110-111	18360.0
112-113	19388.0
114-115	20736.0
116-117	22310.0
118-119	23416.0
120-121	25354.0
122-123	26914.0
124-125	28566.0
126-127	29985.0
128-129	31631.0
130-131	33672.0
132-133	37449.0
134-135	121360.0
136-137	119467.0
138-139	117313.0
140-141	120972.0
142-143	124742.0
144-145	125386.0
146-147	121127.0
148-149	122070.0
150-151	130037.0
152-153	133285.0
154-155	125769.0
156-157	129944.0
158-159	136387.0
160-161	140027.0
162-163	148127.0
164-165	154189.0
166-167	157896.0
168-169	166373.0
170-171	172816.0
172-173	174995.0
174-175	189755.0
176-177	588367.0
178-179	6851420.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.45033866637358
#Duplication Level	Percentage of total
1	87.83906622261325
2	9.867440444019433
3	1.5592744601358879
4	0.4407751282939641
5	0.14557119905867447
6	0.04908955646220776
7	0.030822965865424646
8	0.017547055223375775
9	0.009332474954460093
>10	0.04108049337331675
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.8551060169899884E-5	0.0	9.275530084949942E-6	9.275530084949942E-6	9.275530084949942E-6	0.0
2	1.0203083093444936E-4	0.0	1.8551060169899884E-5	2.7826590254849827E-5	9.275530084949942E-6	0.0
3	1.0203083093444936E-4	9.275530084949942E-6	1.8551060169899884E-5	2.7826590254849827E-5	9.275530084949942E-6	0.0
4	1.0203083093444936E-4	1.8551060169899884E-5	1.8551060169899884E-5	2.7826590254849827E-5	9.275530084949942E-6	0.0
5	1.113063610193993E-4	1.8551060169899884E-5	2.7826590254849827E-5	2.7826590254849827E-5	9.275530084949942E-6	0.0
6	1.113063610193993E-4	3.710212033979977E-5	2.7826590254849827E-5	3.710212033979977E-5	1.8551060169899884E-5	0.0
7	1.113063610193993E-4	4.637765042474971E-5	4.637765042474971E-5	3.710212033979977E-5	1.8551060169899884E-5	0.0
8	1.113063610193993E-4	4.637765042474971E-5	5.565318050969965E-5	4.637765042474971E-5	2.7826590254849827E-5	0.0
9	1.113063610193993E-4	4.637765042474971E-5	5.565318050969965E-5	7.420424067959954E-5	2.7826590254849827E-5	0.0
10-14	1.1687167907036929E-4	6.121849856066962E-5	5.936339254367963E-5	1.0203083093444938E-4	4.82327564417397E-5	0.0
15-19	1.465533753422091E-4	9.090019483250943E-5	8.533487678153947E-5	1.2243699712133924E-4	7.420424067959954E-5	0.0
20-24	1.6695954152909893E-4	1.1501657305337928E-4	1.3727784525725917E-4	1.5768401144414902E-4	7.605934669658953E-5	0.0
25-29	1.9478613178394879E-4	1.5026358737618906E-4	1.762350716140489E-4	1.8922081373297882E-4	9.090019483250943E-5	0.0
30-34	6.196054096746561E-4	1.7252485958006895E-4	1.9478613178394879E-4	2.3188825212374858E-4	1.3542273924026916E-4	0.0
35-39	0.0021760393579292564	1.9107591974996878E-4	2.189025100048186E-4	3.0980270483732807E-4	2.151922979708386E-4	1.6695954152909896E-5
40-44	0.0035877750368586377	2.226127220387986E-4	2.597148423785984E-4	3.4504971916013785E-4	3.5803546127906777E-4	1.8551060169899884E-5
45-49	0.0046915631169676805	2.4858420627665845E-4	2.875414326334482E-4	3.784416274659577E-4	4.7119692831545706E-4	1.8551060169899884E-5
50-54	0.0054985342343583255	2.6156994839558837E-4	2.893965386504382E-4	4.2852948992468733E-4	5.750828652668964E-4	2.7826590254849827E-5
55-59	0.006123704962083952	2.856863266164582E-4	3.09802704837328E-4	4.637765042474971E-4	6.715483781503759E-4	2.7826590254849827E-5
60-64	0.006689512297265898	3.1165781085431806E-4	3.5618035526207783E-4	4.971684125533169E-4	8.422181317134549E-4	2.7826590254849827E-5
65-69	0.0072608849504988155	3.561803552620778E-4	3.654558853470277E-4	5.528215930630167E-4	9.609449168008139E-4	4.637765042474971E-5
70-74	0.014171154863786523	3.8957226356789757E-4	3.765865214489676E-4	5.973441374707763E-4	0.0010796717018881734	4.637765042474971E-5
75-79	0.15239695929572755	4.081233237377975E-4	4.025580056868275E-4	6.196054096746561E-4	0.0011761372147716528	4.637765042474971E-5
80-84	0.21650942324290154	4.396601260266272E-4	4.192539598397374E-4	6.47431999929506E-4	0.0014302867390992811	4.637765042474971E-5
85-89	0.2168136606296879	4.78617352383417E-4	4.5079076212856714E-4	6.826790142523157E-4	0.0018439753808880486	4.637765042474971E-5
90-94	0.2170232876096078	5.082990486552568E-4	4.8603777645137697E-4	7.012300744222157E-4	0.0018866428192788182	4.637765042474971E-5
95-99	0.21727372692190144	5.602420171309765E-4	5.101541546722468E-4	7.587383609489052E-4	0.0019051938794487182	4.637765042474971E-5
100-104	0.2175649785665689	5.750828652668964E-4	5.212847907741867E-4	8.422181317134549E-4	0.001955281741907448	4.637765042474971E-5
105-109	0.2177931566066586	5.954890314537863E-4	5.583869111139866E-4	8.793202520532544E-4	0.0019849634381792873	4.637765042474971E-5
110-114	0.21801762443471442	6.529973179804759E-4	5.843583953518464E-4	8.978713122231544E-4	0.0020165002404681173	4.637765042474971E-5
115-119	0.21836081904785756	6.900994383202757E-4	6.177503036576662E-4	9.201325844270343E-4	0.002062877890892867	4.637765042474971E-5
120-124	0.2197744098328039	7.290566646770654E-4	6.604177420484359E-4	9.461040686648941E-4	0.002109255541317617	4.637765042474971E-5
125-129	0.22253109737405102	7.605934669658954E-4	7.067953924731856E-4	9.665102348517839E-4	0.0021927353120821664	4.637765042474971E-5
130-134	0.22548442615309913	7.97695587305695E-4	7.531730428979352E-4	0.0010370042634974034	0.0022854906129316658	4.637765042474971E-5
135-139	0.22871973104672966	8.16246647475595E-4	7.809996331527851E-4	0.0010685410657862333	0.0023467091114923355	4.637765042474971E-5
140-144	0.23194761551629223	8.774651460362645E-4	8.10681329424625E-4	0.0010889472319731233	0.002417203140137955	4.637765042474971E-5
145-149	0.23391402789430163	9.479591746818841E-4	8.273772835775348E-4	0.001122339140278943	0.0024802767447156147	4.637765042474971E-5
150-154	0.23578582986544455	0.0010036123551915837	8.644794039173346E-4	0.0011817025328226226	0.002521089077089394	4.637765042474971E-5
155-159	0.23855550314881055	0.0010499900056163336	8.811753580702447E-4	0.0012150944411284425	0.0026305403320918035	4.637765042474971E-5
160-164	0.24136042344649944	0.0010833819139221532	8.904508881551945E-4	0.0012447761374002823	0.002667642452431603	4.637765042474971E-5
165-167	0.2440020944146932	0.0010945125500240933	8.935427315168444E-4	0.001267655778276492	0.002696087411358783	4.637765042474971E-5
>>END_MODULE
