##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479166
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131192
Total Bases	187.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.05112571517479	41.0	40.0	41.0	38.0	41.0
2	40.09424217993055	41.0	40.0	41.0	38.0	41.0
3	40.16410565138367	41.0	40.0	41.0	38.0	41.0
4	40.15843199032525	41.0	40.0	41.0	38.0	41.0
5	40.161028366537245	41.0	40.0	41.0	38.0	41.0
6	40.14048278276367	41.0	40.0	41.0	38.0	41.0
7	40.12239213148608	41.0	40.0	41.0	38.0	41.0
8	40.07820069448864	41.0	40.0	41.0	38.0	41.0
9	40.04372290468815	41.0	40.0	41.0	38.0	41.0
10-14	39.941844708944195	41.0	40.0	41.0	38.0	41.0
15-19	39.83429815628116	41.0	40.0	41.0	37.6	41.0
20-24	39.59753693449034	40.8	39.2	41.0	36.6	41.0
25-29	39.120786745309374	40.0	38.0	41.0	35.0	41.0
30-34	38.40672759354734	39.6	36.2	41.0	35.0	41.0
35-39	37.486278014696	38.0	35.0	40.4	34.0	41.0
40-44	36.67892276465888	36.0	35.0	39.2	33.8	41.0
45-49	35.93133455682148	35.0	35.0	37.4	33.4	40.0
50-54	35.365251168678704	35.0	35.0	36.2	33.0	38.8
55-59	35.03751953691327	35.0	35.0	35.0	33.0	37.0
60-64	34.40140930982538	34.6	33.8	35.0	31.8	36.4
65-69	34.967176394458235	35.0	35.0	35.0	33.0	36.0
70-74	34.97614321883465	35.0	35.0	35.0	33.0	37.0
75-79	34.9911160970021	35.0	35.0	35.0	33.0	38.0
80-84	35.00020473977893	35.0	35.0	35.0	33.0	39.0
85-89	35.720106754644654	36.0	34.8	36.2	33.0	39.6
90-94	39.664431448796066	41.0	40.0	41.0	36.4	41.0
95-99	39.48367011844564	41.0	39.0	41.0	35.4	41.0
100-104	39.313879221497224	41.0	39.0	41.0	35.0	41.0
105-109	39.07349315051583	40.0	38.0	41.0	35.0	41.0
110-114	38.67295136383294	40.0	37.0	41.0	35.0	41.0
115-119	38.08443755663343	39.4	35.4	41.0	34.0	41.0
120-124	36.7593239641978	37.2	34.4	39.6	33.0	40.4
125-129	36.40454063933201	35.8	35.0	39.0	33.0	40.8
130-134	35.6989620243459	35.0	35.0	37.4	33.0	39.8
135-139	35.281362674814844	35.0	35.0	36.2	33.0	38.6
140-144	34.84514071787275	35.0	35.0	35.0	33.0	36.6
145-149	34.563671909115456	35.0	34.8	35.0	33.0	35.8
150-154	34.37624587195489	35.0	34.0	35.0	33.0	35.0
155-159	34.257905556341456	35.0	34.0	35.0	33.0	35.0
160-164	34.1619620800341	35.0	34.0	35.0	32.4	35.0
165-169	34.06351083888986	35.0	34.0	35.0	32.0	35.0
170-174	33.960251547381674	35.0	33.6	35.0	31.4	35.0
175-176	33.28459929519399	34.5	32.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	28.0
31	383.0
32	2512.0
33	11456.0
34	41247.0
35	142230.0
36	408541.0
37	442324.0
38	80961.0
39	1503.0
40	7.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.110075035891345	22.547454366721123	23.29197872686511	27.050491870522425
2	26.626779538752043	22.701451212526255	23.54436735761922	27.127401891102483
3	26.655598695888937	22.987963139767608	23.43130078713428	26.925137377209175
4	26.898439875812418	22.94544162264231	23.05134760500428	27.10477089654099
5	26.81931979716971	23.12931845345441	23.238141712459072	26.813220036916807
6	26.6261607224945	23.136213834609862	23.34758378772127	26.89004165517436
7	26.88968804588434	22.981067758612152	23.043391395978755	27.08585279952475
8	27.047839800847246	23.09846604289988	23.04542464939639	26.808269506856487
9	26.676550046322816	22.99530053253559	23.22435095014816	27.10379847099343
10-14	26.84242816427273	22.99333800097596	23.11993012680429	27.044303707947016
15-19	26.758180750924687	22.93849320009335	23.196928549706858	27.106397499275104
20-24	26.784436240708914	22.961778371841383	23.127090714927263	27.12669467252244
25-29	26.831395554424002	23.00120580767898	23.059904949822844	27.107493688074175
30-34	26.74290482959568	22.98099703675415	23.059904949822844	27.216193183827325
35-39	26.699923620393356	23.02295277901541	23.07887608823259	27.19824751235864
40-44	26.9133975487804	22.880925607677565	22.92159067603024	27.284086167511795
45-49	27.10107567946025	22.860982043720252	22.800231967694256	27.23771030912524
50-54	27.075757254294587	22.921838202533255	22.81504819694623	27.187356346225926
55-59	27.087744609226373	22.906933570958778	22.78005855769843	27.22526326211642
60-64	26.98083084038784	22.91667550689892	22.859373121450645	27.243120531262598
65-69	26.963963677253727	22.999331678441855	22.82339337619078	27.21331126811364
70-74	26.98254584544445	22.964518843839066	22.802229860182887	27.250705450533598
75-79	26.927701044561843	23.00893217066599	22.775178749496106	27.28818803527606
80-84	26.886929893422163	23.049774043663675	22.81432329790168	27.248972765012482
85-89	26.900915140842578	22.958737331947184	22.775815246218148	27.364532280992087
90-94	26.528329894631963	23.218299090446244	23.272359826277054	26.981011188644732
95-99	26.74965074542764	23.191194233709172	23.055494510651357	27.003660510211837
100-104	26.61999118908469	23.1814791079916	23.13888058759323	27.059649115330476
105-109	26.683322092436363	23.092974252195095	23.14708388312106	27.076619772247483
110-114	26.711758014188668	23.13315871681229	23.002088803539927	27.15299446545912
115-119	26.700345588508416	23.099236156715357	23.051867847646783	27.148550407129445
120-124	26.642783240744645	23.19094219588502	23.003197615701957	27.16307694766838
125-129	26.762639517864955	23.093729180881994	22.907735898700633	27.23589540255242
130-134	26.9497709552	23.01764069219958	22.742186669196172	27.290401683404248
135-139	27.05092425870628	23.05204769352208	22.758859541356014	27.13816850641562
140-144	27.056331143675994	23.04740153771261	22.738147920430464	27.158119398180936
145-149	26.983903334258784	23.124549673392238	22.780771745858246	27.110775246490732
150-154	26.99809658943057	23.15416972342515	22.801360456226238	27.046373230918043
155-159	26.981384008890373	23.249227771037685	22.77374451235829	26.99564370771365
160-164	26.923193883313782	23.30775060215957	22.79457549560508	26.974480018921565
165-169	26.938620487129523	23.438768861110127	22.802637919657933	26.81997273210242
170-174	26.917754249902337	23.50914499523577	22.826438205020093	26.746662549841798
175-176	26.832746852334665	23.513800983603105	22.775948211145195	26.87750395291704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	1.0
24	3.5
25	10.5
26	21.0
27	46.5
28	78.5
29	140.0
30	225.5
31	369.0
32	613.0
33	957.5
34	1391.0
35	1966.0
36	2768.5
37	3880.0
38	5470.5
39	7306.5
40	9658.5
41	12751.5
42	16316.5
43	20072.0
44	24215.5
45	29136.5
46	34180.0
47	38661.0
48	42821.5
49	47118.5
50	50869.0
51	53697.0
52	55846.0
53	56900.5
54	56938.0
55	56322.0
56	54806.5
57	52362.5
58	49357.0
59	46127.0
60	42610.5
61	39140.0
62	35661.0
63	31893.0
64	28258.5
65	24671.0
66	20963.5
67	17515.0
68	14363.0
69	11519.0
70	9015.0
71	6778.5
72	5058.0
73	3673.0
74	2502.5
75	1652.0
76	1062.5
77	674.0
78	420.5
79	228.0
80	126.0
81	77.5
82	38.5
83	16.5
84	7.5
85	4.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	189.0
92-93	430.0
94-95	694.0
96-97	897.0
98-99	1171.0
100-101	1427.0
102-103	1631.0
104-105	1879.0
106-107	2110.0
108-109	2220.0
110-111	2469.0
112-113	2579.0
114-115	2814.0
116-117	2961.0
118-119	3196.0
120-121	3376.0
122-123	3693.0
124-125	3805.0
126-127	4096.0
128-129	4285.0
130-131	4326.0
132-133	11060.0
134-135	16745.0
136-137	16882.0
138-139	16782.0
140-141	16700.0
142-143	16365.0
144-145	16432.0
146-147	18286.0
148-149	16472.0
150-151	16659.0
152-153	16908.0
154-155	17452.0
156-157	17872.0
158-159	18237.0
160-161	19110.0
162-163	20215.0
164-165	20481.0
166-167	21030.0
168-169	21759.0
170-171	22405.0
172-173	23105.0
174-175	58051.0
176-177	641936.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.21203647413039
#Duplication Level	Percentage of total
1	98.43906230020512
2	1.5171118310267284
3	0.04154149463822495
4	0.00228437412991636
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	8.840232250581688E-5	0.0	0.0	0.0	0.0	0.0
7	8.840232250581688E-5	0.0	0.0	0.0	0.0	0.0
8	8.840232250581688E-5	0.0	0.0	0.0	0.0	0.0
9	8.840232250581688E-5	8.840232250581688E-5	0.0	0.0	0.0	0.0
10-14	8.840232250581688E-5	8.840232250581688E-5	7.072185800465351E-5	3.5360929002326754E-5	0.0	0.0
15-19	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0	0.0
20-24	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0	0.0
25-29	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0	0.0
30-34	2.121655740139605E-4	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0	0.0
35-39	5.304139350349013E-4	1.0608278700698025E-4	8.840232250581688E-5	8.840232250581688E-5	7.072185800465351E-5	0.0
40-44	9.547450830628224E-4	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0
45-49	0.0010431474055686393	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0
50-54	0.0012553129795825997	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	0.0
55-59	0.0013437153020884164	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5	8.840232250581688E-5	3.5360929002326754E-5
60-64	0.00141443716009307	1.7680464501163375E-4	2.8288743201861403E-4	1.2376325150814362E-4	8.840232250581688E-5	8.840232250581688E-5
65-69	0.0015028394825988868	1.7680464501163375E-4	3.536092900232675E-4	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5
70-74	0.004066506835267577	1.7680464501163375E-4	4.066506835267576E-4	1.7680464501163375E-4	8.840232250581688E-5	8.840232250581688E-5
75-79	0.022383468058472834	1.7680464501163375E-4	4.420116125290844E-4	1.7680464501163375E-4	8.840232250581688E-5	1.0608278700698025E-4
80-84	0.026697501396756695	1.7680464501163375E-4	4.420116125290844E-4	2.4752650301628723E-4	1.2376325150814362E-4	1.7680464501163375E-4
85-89	0.026750542790260186	1.7680464501163375E-4	4.5969207703024773E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
90-94	0.026874306041768332	1.7680464501163375E-4	6.011357930395549E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
95-99	0.026874306041768332	1.7680464501163375E-4	6.188162575407181E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
100-104	0.026962708364274147	1.7680464501163375E-4	6.188162575407181E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
105-109	0.027051110686779962	1.7680464501163375E-4	6.188162575407181E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
110-114	0.02712183254478462	2.4752650301628723E-4	6.895381155453716E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
115-119	0.02713951300928578	2.6520696751745063E-4	7.07218580046535E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
120-124	0.02724559579629276	2.6520696751745063E-4	7.07218580046535E-4	2.6520696751745063E-4	1.7680464501163375E-4	1.7680464501163375E-4
125-129	0.027563844157313704	2.6520696751745063E-4	7.07218580046535E-4	2.8288743201861403E-4	1.7680464501163375E-4	1.7680464501163375E-4
130-134	0.02802353623434395	2.6520696751745063E-4	7.602599735500251E-4	3.536092900232675E-4	1.7680464501163375E-4	1.7680464501163375E-4
135-139	0.028695393885388155	2.6520696751745063E-4	7.95620902552352E-4	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
140-144	0.028995961781907935	2.6520696751745063E-4	8.840232250581688E-4	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
145-149	0.02927884921392655	2.6520696751745063E-4	8.840232250581688E-4	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
150-154	0.02980926314896145	2.6520696751745063E-4	8.840232250581688E-4	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
155-159	0.030039109187476577	3.889702190255942E-4	9.017036895593321E-4	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
160-164	0.03076400823202427	5.304139350349013E-4	0.0010608278700698025	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
165	0.031029215199541722	5.304139350349013E-4	0.0010608278700698025	5.304139350349013E-4	1.7680464501163375E-4	1.7680464501163375E-4
>>END_MODULE
