##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479169
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230676
Total Bases	208.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.16896648671137	39.0	38.0	39.0	37.0	39.0
2	39.82831712002184	41.0	40.0	41.0	38.0	41.0
3	39.89053820826928	41.0	40.0	41.0	38.0	41.0
4	39.91935732881766	41.0	40.0	41.0	38.0	41.0
5	39.90967647049264	41.0	40.0	41.0	38.0	41.0
6	39.89117931933344	41.0	40.0	41.0	38.0	41.0
7	39.914250379466246	41.0	40.0	41.0	38.0	41.0
8	39.88347461070176	41.0	40.0	41.0	38.0	41.0
9	39.86024997643572	41.0	40.0	41.0	38.0	41.0
10-14	39.769842915600854	40.2	40.0	41.0	37.8	41.0
15-19	39.706019618486096	40.2	39.8	41.0	37.6	41.0
20-24	39.695735351952905	40.4	40.0	41.0	37.0	41.0
25-29	39.51616607458014	40.0	39.0	41.0	36.6	41.0
30-34	39.228959043647556	40.0	38.4	41.0	35.4	41.0
35-39	38.816149660836814	40.0	37.8	41.0	35.0	41.0
40-44	38.29129340297528	39.4	36.4	41.0	34.0	41.0
45-49	37.84155212257328	38.8	35.4	40.6	34.0	41.0
50-54	37.16995569914421	37.2	35.0	39.8	34.0	41.0
55-59	36.43796141307704	36.0	35.0	39.0	33.0	40.6
60-64	35.480563202662594	34.8	34.4	37.0	32.4	39.6
65-69	35.184522165054005	35.0	34.4	36.0	32.8	39.0
70-74	35.09614212026561	35.0	34.4	36.0	33.0	37.8
75-79	34.93456571835316	35.0	34.0	35.2	33.0	37.0
80-84	34.90071944199773	35.0	34.0	35.0	33.0	38.0
85-89	34.60895410327333	34.8	33.4	35.0	32.0	38.6
90-94	39.40565651463677	40.6	39.0	40.6	36.8	40.8
95-99	39.60292433036106	40.8	39.0	41.0	37.0	41.0
100-104	39.4250387645626	40.0	39.0	41.0	36.0	41.0
105-109	39.408303979976296	40.0	39.0	41.0	36.0	41.0
110-114	39.264936876636035	40.0	38.6	41.0	35.0	41.0
115-119	39.03498775289084	40.0	38.0	41.0	35.0	41.0
120-124	38.73039040297759	40.0	37.6	41.0	34.8	41.0
125-129	38.026656060468596	39.4	36.4	40.4	33.8	40.6
130-134	38.12106605117099	39.0	35.6	40.8	34.0	41.0
135-139	37.650322161055286	38.4	35.0	40.8	34.0	41.0
140-144	36.965087214772566	36.8	35.0	39.6	34.0	41.0
145-149	36.248868933000814	35.6	35.0	38.4	33.4	40.6
150-154	35.57387864198232	35.0	35.0	36.8	33.0	39.0
155-159	35.039943510912885	35.0	35.0	36.0	33.0	37.2
160-164	34.675397895457465	35.0	34.0	35.0	33.0	36.2
165-169	34.44971389630254	35.0	34.0	35.0	33.0	36.0
170-174	34.30613257443226	35.0	34.0	35.0	32.6	35.6
175-178	33.825102263256504	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	25.0
31	368.0
32	2662.0
33	11764.0
34	36688.0
35	93316.0
36	221916.0
37	411902.0
38	384382.0
39	67361.0
40	289.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.336159964117282	28.043530547438966	28.51059092726274	22.109718561181012
2	21.952162876337884	28.62443080063315	29.142763814358936	20.280642508670034
3	20.653608260825756	27.58126428076927	29.55684518102246	22.208282277382512
4	21.047863125631768	26.9875255550608	30.003022728971718	21.96158859033572
5	21.704331603118938	27.60710373810816	28.720475576024885	21.96808908274802
6	22.12962631919368	27.47327485056993	28.17979711963181	22.217301710604577
7	21.9633762257491	28.031748404941677	27.958374096837836	22.04650127247139
8	21.774536921171777	28.269910195697324	28.223187906483915	21.73236497664698
9	22.291488580259955	27.9218900831738	27.7184246706688	22.068196665897442
10-14	21.99610620504503	28.135756283538477	27.86705842967605	22.00107908174044
15-19	22.085195453555606	28.0554914534776	27.81292557911262	22.046387513854175
20-24	21.90981216827175	28.01286447448394	28.03807013381264	22.039253223431675
25-29	21.943890999743232	28.04465188238009	27.915844625230363	22.095612492646318
30-34	22.038976952504154	28.012506947401267	27.858136503840164	22.090379596254415
35-39	21.902287848304507	28.063340798065457	27.898748330186013	22.135623023444026
40-44	21.95193535910345	28.104521417497374	27.831305721408395	22.112237501990776
45-49	22.25217685239657	27.907345231401276	27.671052332214163	22.169425583987987
50-54	22.151191702771484	27.959657944089265	27.78160945691636	22.107540896222886
55-59	22.07445338984428	28.031358375396938	27.77036360504308	22.123824629715703
60-64	22.079068739457014	27.957236510665684	27.7738088660216	22.189885883855702
65-69	22.052692991494105	27.95871537268948	27.82865677075038	22.159934865066027
70-74	21.97977371785913	28.06927249739168	27.774816442345507	22.17613734240369
75-79	22.0239933174938	27.98245842122541	27.728939217145697	22.264609044135092
80-84	22.003516766395055	28.031845912327856	27.754648664636346	22.209988656640743
85-89	21.961702348952933	28.05440262099854	27.70365230166185	22.280242728386675
90-94	21.285433394345223	27.760451976666488	29.068049248799603	21.886065380188686
95-99	21.82699615224854	28.02205660192246	28.01242461382968	22.13852263199932
100-104	21.91341378714413	28.07365730067791	27.920466810357475	22.09246210182048
105-109	21.79860649148386	28.068380949016973	28.046010506258817	22.087002053240347
110-114	21.9198383802825	27.925999606590494	27.977261062015195	22.176900951111808
115-119	21.874673573231394	28.021356664716702	27.946289852943966	22.157679909107937
120-124	21.828350467036863	28.031274555661824	27.972531852023497	22.16784312527782
125-129	21.894885682266985	27.973000640971534	27.952526322035133	22.179587354726348
130-134	21.943133025153077	27.985859925029764	27.957450364993758	22.113556684823397
135-139	21.950977015982406	28.05303088422535	27.904669771384555	22.091322328407685
140-144	21.981904076721612	28.061297902276344	27.932347614676623	22.02445040632542
145-149	21.871255697101752	28.119315815551353	28.013855761153277	21.99557272619362
150-154	21.767935365616577	28.23862370453632	28.07848017353047	21.91496075631663
155-159	21.76024907759038	28.2628860059737	28.082300826757777	21.89456408967814
160-164	21.656303278998333	28.439097093041667	28.13715425154276	21.767445376417243
165-169	21.575138957988948	28.560518277188425	28.161645990145885	21.702696774676745
170-174	21.546211641946194	28.679837674374287	28.2108959744166	21.563054709262918
175-178	21.40062541879365	28.89822937923185	28.264111540013936	21.437033661960562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.5
11	5.5
12	11.0
13	23.0
14	42.0
15	71.5
16	140.5
17	242.0
18	403.0
19	644.5
20	1018.0
21	1556.5
22	2231.5
23	3195.5
24	4470.5
25	5919.0
26	7763.5
27	9819.0
28	11972.5
29	14488.0
30	17236.0
31	20216.0
32	23275.5
33	26339.5
34	29845.5
35	33505.0
36	37076.5
37	40593.5
38	43934.0
39	47163.5
40	49694.5
41	51683.5
42	53170.5
43	53874.0
44	53652.5
45	52449.5
46	50639.5
47	48181.5
48	44938.0
49	41503.0
50	38240.5
51	34685.0
52	31116.5
53	28045.0
54	25086.0
55	22530.5
56	20562.0
57	18653.0
58	16937.0
59	15531.0
60	14224.5
61	12970.5
62	11684.5
63	10423.5
64	9257.5
65	8040.0
66	6851.0
67	5646.0
68	4517.0
69	3563.0
70	2743.0
71	2058.0
72	1487.0
73	1020.5
74	716.0
75	481.0
76	290.5
77	183.5
78	124.0
79	68.5
80	36.0
81	23.0
82	6.5
83	1.5
84	0.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	156.0
92-93	355.0
94-95	511.0
96-97	778.0
98-99	979.0
100-101	1169.0
102-103	1260.0
104-105	1531.0
106-107	1733.0
108-109	1989.0
110-111	2090.0
112-113	2328.0
114-115	2549.0
116-117	2522.0
118-119	2818.0
120-121	2936.0
122-123	3167.0
124-125	3384.0
126-127	3567.0
128-129	3790.0
130-131	4069.0
132-133	4449.0
134-135	13066.0
136-137	13508.0
138-139	13487.0
140-141	13709.0
142-143	14265.0
144-145	13942.0
146-147	14034.0
148-149	14529.0
150-151	15240.0
152-153	15693.0
154-155	14405.0
156-157	15366.0
158-159	15967.0
160-161	16138.0
162-163	17259.0
164-165	17928.0
166-167	18434.0
168-169	19582.0
170-171	19959.0
172-173	20863.0
174-175	21889.0
176-177	82814.0
178-179	760469.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.34940208683109
#Duplication Level	Percentage of total
1	98.71752599722706
2	1.2366320954765324
3	0.03147437796651545
4	0.010169778153649408
5	0.0011757632480022514
6	0.0
7	0.001270716036419735
8	0.0
9	0.0
>10	0.0017512718918302342
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	1.6251231030750578E-4	0.0	0.0	0.0	0.0	0.0
3	1.6251231030750578E-4	0.0	0.0	0.0	0.0	0.0
4	2.437684654612587E-4	0.0	0.0	0.0	0.0	0.0
5	2.437684654612587E-4	0.0	0.0	0.0	0.0	0.0
6	2.437684654612587E-4	0.0	8.125615515375289E-5	8.125615515375289E-5	0.0	0.0
7	2.437684654612587E-4	0.0	8.125615515375289E-5	8.125615515375289E-5	0.0	0.0
8	2.437684654612587E-4	0.0	8.125615515375289E-5	8.125615515375289E-5	0.0	0.0
9	2.437684654612587E-4	0.0	8.125615515375289E-5	8.125615515375289E-5	0.0	0.0
10-14	2.437684654612587E-4	0.0	8.125615515375289E-5	1.9501477236900697E-4	4.8753693092251735E-5	0.0
15-19	2.437684654612587E-4	0.0	8.125615515375289E-5	2.925221585535104E-4	8.125615515375289E-5	0.0
20-24	2.437684654612587E-4	0.0	1.6251231030750578E-4	4.875369309225174E-4	8.125615515375289E-5	0.0
25-29	3.2502462061501157E-4	0.0	1.6251231030750578E-4	5.687930860762703E-4	1.3000984824600463E-4	0.0
30-34	4.875369309225174E-4	3.250246206150116E-5	1.6251231030750578E-4	5.687930860762703E-4	2.6001969649200925E-4	0.0
35-39	0.001088832479060289	1.6251231030750578E-4	1.7876354133825636E-4	5.850443171070208E-4	3.2502462061501157E-4	0.0
40-44	0.001657625565136559	1.6251231030750578E-4	3.2502462061501157E-4	6.825517032915243E-4	4.387832378302656E-4	0.0
45-49	0.0019501477236900696	1.6251231030750578E-4	3.4127585164576215E-4	7.313053963837761E-4	5.03788161953268E-4	8.125615515375289E-5
50-54	0.0023401772684280833	2.437684654612587E-4	4.062807757687644E-4	7.313053963837761E-4	5.850443171070208E-4	2.2751723443050812E-4
55-59	0.002941472816565855	2.437684654612587E-4	4.2253200679951504E-4	7.313053963837761E-4	6.98802934322275E-4	3.2502462061501157E-4
60-64	0.0031202363579041115	2.437684654612587E-4	4.875369309225174E-4	7.313053963837761E-4	7.800590894760279E-4	3.2502462061501157E-4
65-69	0.003461512209549873	2.437684654612587E-4	4.875369309225174E-4	7.313053963837761E-4	8.613152446297807E-4	3.2502462061501157E-4
70-74	0.005931699326223961	3.2502462061501157E-4	4.875369309225174E-4	7.313053963837761E-4	8.93817706691282E-4	3.2502462061501157E-4
75-79	0.05059008219872655	3.4127585164576215E-4	5.03788161953268E-4	7.313053963837761E-4	8.93817706691282E-4	3.2502462061501157E-4
80-84	0.07124539683881055	4.062807757687644E-4	5.687930860762703E-4	7.475566274145267E-4	9.913250928757853E-4	3.2502462061501157E-4
85-89	0.0713266529939643	4.387832378302656E-4	5.687930860762703E-4	8.125615515375288E-4	0.0013813546376137992	3.2502462061501157E-4
90-94	0.07147291407324105	5.200393929840186E-4	5.687930860762703E-4	8.125615515375288E-4	0.0013813546376137992	3.2502462061501157E-4
95-99	0.07158667269045631	6.012955481377715E-4	5.687930860762703E-4	8.775664756605312E-4	0.0013813546376137992	3.2502462061501157E-4
100-104	0.07161917515251781	7.150541653530256E-4	5.687930860762703E-4	8.93817706691282E-4	0.0014138570996753007	3.2502462061501157E-4
105-109	0.07176543623179457	7.313053963837761E-4	5.687930860762703E-4	8.93817706691282E-4	0.0014626107927675522	3.2502462061501157E-4
110-114	0.07187919484900981	7.313053963837761E-4	5.687930860762703E-4	9.425713997835335E-4	0.0014626107927675522	3.2502462061501157E-4
115-119	0.07191169731107132	7.313053963837761E-4	5.687930860762703E-4	0.0010563300169987876	0.0014626107927675522	3.2502462061501157E-4
120-124	0.07231797808684008	7.313053963837761E-4	5.687930860762703E-4	0.0010563300169987876	0.0015276157168905547	3.2502462061501157E-4
125-129	0.0728380174798241	7.638078584452772E-4	5.687930860762703E-4	0.0010563300169987876	0.0015438669479213051	3.2502462061501157E-4
130-134	0.07379684011063839	8.125615515375288E-4	5.850443171070208E-4	0.0010563300169987876	0.001657625565136559	3.2502462061501157E-4
135-139	0.07522694844134444	8.125615515375288E-4	6.500492412300231E-4	0.0010563300169987876	0.001820137875444065	3.2502462061501157E-4
140-144	0.07706333754781924	8.125615515375288E-4	6.500492412300231E-4	0.0012188423273062935	0.0018688915685363167	3.2502462061501157E-4
145-149	0.07824967741306404	8.125615515375288E-4	6.500492412300231E-4	0.0013000984824600463	0.0019501477236900696	3.2502462061501157E-4
150-154	0.07896473157841707	8.125615515375288E-4	6.500492412300231E-4	0.001316349713490797	0.0020476551098745728	3.2502462061501157E-4
155-159	0.07954977589552409	8.125615515375288E-4	6.500492412300231E-4	0.0013813546376137992	0.002193916189151328	3.2502462061501157E-4
160-164	0.08057360345046137	8.125615515375288E-4	6.500492412300231E-4	0.0013813546376137992	0.0024214334235818365	3.2502462061501157E-4
165-167	0.0815811797743679	8.125615515375289E-4	6.500492412300231E-4	0.0013813546376137992	0.002437684654612587	3.2502462061501157E-4
>>END_MODULE
