##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479171
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7281777
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.19111749783055	39.0	38.0	39.0	37.0	39.0
2	39.87523594858782	41.0	40.0	41.0	38.0	41.0
3	39.90856833435026	41.0	40.0	41.0	38.0	41.0
4	39.90775273672896	41.0	40.0	41.0	38.0	41.0
5	39.90354181403797	41.0	40.0	41.0	38.0	41.0
6	39.88554002683686	41.0	40.0	41.0	38.0	41.0
7	39.90058031164646	41.0	40.0	41.0	38.0	41.0
8	39.87296740891681	41.0	40.0	41.0	38.0	41.0
9	39.84191880635729	41.0	40.0	41.0	38.0	41.0
10-14	39.749309461138395	40.6	39.8	41.0	37.8	41.0
15-19	39.681969497280676	40.6	39.6	41.0	37.0	41.0
20-24	39.66167788439553	40.6	39.4	41.0	37.0	41.0
25-29	39.49257611157277	40.0	39.0	41.0	36.6	41.0
30-34	39.21840624891424	40.0	38.4	41.0	35.4	41.0
35-39	38.822098067545866	40.0	37.8	41.0	35.0	41.0
40-44	38.34741440173188	39.6	36.6	41.0	34.2	41.0
45-49	37.90654536660488	39.0	35.4	40.8	34.0	41.0
50-54	37.25000834274381	37.4	35.0	40.0	34.0	41.0
55-59	36.52305032137073	36.2	35.0	39.0	33.2	40.8
60-64	35.59846356184761	35.0	34.6	37.2	32.4	39.6
65-69	35.26754455677509	35.0	34.6	36.2	32.8	39.0
70-74	35.148155484574716	35.0	35.0	36.0	33.0	38.2
75-79	34.98448897844578	35.0	34.0	35.4	33.0	37.2
80-84	34.92700806410304	35.0	34.0	35.0	33.0	38.0
85-89	34.664866474213646	34.8	33.6	35.0	32.2	38.6
90-94	39.3467728876728	40.6	38.8	40.6	36.8	40.8
95-99	39.548996757440584	40.6	39.0	41.0	36.6	41.0
100-104	39.36977596362461	40.0	39.0	41.0	36.0	41.0
105-109	39.359518339062944	40.0	39.0	41.0	35.8	41.0
110-114	39.20888456225327	40.0	38.2	41.0	35.0	41.0
115-119	38.97722461013599	40.0	38.0	41.0	35.0	41.0
120-124	38.67755510833206	40.0	37.6	41.0	34.6	41.0
125-129	38.03981925524037	39.4	36.4	40.4	33.8	40.6
130-134	38.07148647482457	39.0	35.6	40.8	34.0	41.0
135-139	37.616817559482236	38.2	35.0	40.8	34.0	41.0
140-144	36.948099158797405	36.8	35.0	39.6	34.0	41.0
145-149	36.240265300940386	35.6	35.0	38.4	33.0	40.6
150-154	35.56739639672902	35.0	35.0	36.8	33.0	39.0
155-159	35.02901720945614	35.0	34.8	36.0	33.0	37.4
160-164	34.651357162030415	35.0	34.0	35.0	33.0	36.4
165-169	34.412375506166185	35.0	34.0	35.0	33.0	36.0
170-174	34.26116497859102	35.0	34.0	35.0	32.0	35.4
175-178	33.76813196127858	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	169.0
31	2261.0
32	16238.0
33	71694.0
34	225170.0
35	564486.0
36	1306561.0
37	2387045.0
38	2277641.0
39	428500.0
40	2009.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.184719059647115	28.074301094361992	28.696951307352585	22.0440285386383
2	21.902085164102115	28.65060273062468	29.203640814597865	20.243671290675337
3	20.57630987600966	27.582539262051007	29.709272887648165	22.131877974291164
4	21.00925364783898	27.012183976521115	30.061631934073237	21.916930441566667
5	21.661924005637633	27.699832060223763	28.748669452525117	21.889574481613487
6	22.081903908894766	27.477400090664684	28.26634213049919	22.174353869941363
7	21.963828334759498	28.012681519909222	28.016046083256875	22.00744406207441
8	21.7997475066869	28.31159207429725	28.218249473995154	21.670410945020702
9	22.195214162696825	28.014123475629642	27.73007193161779	22.06059043005574
10-14	21.94030111056683	28.20914455358905	27.914455496233952	21.936098839610167
15-19	22.00829550259504	28.06963465099247	27.912439504807686	22.0096303416048
20-24	21.902722371201424	28.045121403745267	28.075325569569078	21.976830655484232
25-29	21.88132100172801	28.09936915123877	27.972496273917752	22.046813573115465
30-34	21.939831444989323	28.08023920534782	27.92341759435918	22.056511755303685
35-39	21.84551106137966	28.096946665628458	27.980321286960585	22.077220986031293
40-44	21.893131305723866	28.119902600697603	27.912579580506243	22.074386513072287
45-49	22.170173571643296	27.939875115648285	27.757353733848205	22.132597578860217
50-54	22.06734976915662	28.04387994853454	27.84926536475918	22.03950491754966
55-59	22.01375021509173	28.06725611069935	27.834227826531905	22.084765847677016
60-64	22.03106467006611	28.026845644957266	27.824790569664522	22.1172991153121
65-69	21.977786466133196	28.039089908960406	27.86912590154848	22.11399772335791
70-74	21.912917684790404	28.133415785734716	27.845486067480508	22.108180461994372
75-79	21.968258572049105	28.070876106203198	27.796701272230667	22.164164049517034
80-84	21.922830100399942	28.129392042629153	27.816671672312953	22.13110618465795
85-89	21.88415272810469	28.18175288806565	27.751530979320023	22.182563404509644
90-94	21.255242175954265	27.794877367950154	29.09822774819051	21.851652707905068
95-99	21.80136945331187	28.03459729423307	28.08561042704144	22.07842282541361
100-104	21.857353911603973	28.083825908584494	27.997581484870388	22.061238694941146
105-109	21.782987374173626	28.096240654059347	28.07977237722507	22.040999594541955
110-114	21.828679893620063	27.96554961362619	28.090573282043596	22.115197210710154
115-119	21.813189150396646	28.043772472993332	28.024460463943733	22.118577912666286
120-124	21.78343525839139	28.1007144952701	28.02151896526677	22.094331281071735
125-129	21.849483058602814	28.046480956104354	28.00344387183287	22.100592113459964
130-134	21.878310406280324	28.052484063848187	28.007066687139897	22.06213884273159
135-139	21.913745449851984	28.07759592974593	27.976289633811042	22.032368986591045
140-144	21.92500552185957	28.1087452749862	27.99299602910392	21.97325317405031
145-149	21.831785919226878	28.18287336768257	28.05773827282711	21.927602440263446
150-154	21.722322913197704	28.320276233504554	28.119474703119167	21.83792615017857
155-159	21.725375391926054	28.32946555193066	28.115882611492708	21.82927644465058
160-164	21.601138801241685	28.487185362939684	28.21122272934945	21.700453106469187
165-169	21.50564851858481	28.599856319971757	28.261414180361378	21.633080981082056
170-174	21.49371385227147	28.70635444901489	28.278573929798224	21.521357768915426
175-178	21.34203028939871	28.892882282867667	28.36207327435398	21.40301415337965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	3.0
10	8.5
11	32.5
12	69.5
13	123.5
14	238.5
15	438.5
16	817.5
17	1450.5
18	2510.0
19	4051.0
20	6372.5
21	9686.5
22	14001.5
23	19717.0
24	27141.5
25	36206.5
26	46809.0
27	59040.0
28	72790.0
29	87602.5
30	104034.5
31	121854.0
32	140252.0
33	159243.5
34	179406.0
35	200423.0
36	221060.0
37	241796.5
38	261719.0
39	279587.0
40	294595.5
41	306720.5
42	315960.5
43	319347.5
44	317399.0
45	311005.0
46	299509.0
47	284213.5
48	265849.0
49	245400.5
50	223818.5
51	202002.0
52	181474.5
53	163114.5
54	146547.5
55	131921.5
56	119269.5
57	108068.0
58	98773.5
59	90633.0
60	82634.5
61	74542.0
62	67159.5
63	60458.0
64	53219.5
65	46328.5
66	39468.0
67	32510.5
68	26399.5
69	20947.0
70	16022.5
71	11941.5
72	8812.5
73	6286.5
74	4288.5
75	2859.5
76	1840.5
77	1152.5
78	687.0
79	405.5
80	238.0
81	132.0
82	70.5
83	31.0
84	11.5
85	4.5
86	1.5
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1026.0
92-93	2348.0
94-95	3541.0
96-97	4916.0
98-99	6026.0
100-101	7229.0
102-103	8459.0
104-105	9492.0
106-107	10555.0
108-109	12162.0
110-111	13193.0
112-113	14009.0
114-115	15162.0
116-117	16054.0
118-119	17129.0
120-121	18427.0
122-123	19210.0
124-125	20757.0
126-127	21889.0
128-129	22584.0
130-131	24207.0
132-133	27012.0
134-135	81987.0
136-137	85714.0
138-139	83455.0
140-141	84609.0
142-143	89666.0
144-145	88938.0
146-147	85752.0
148-149	86478.0
150-151	92146.0
152-153	96242.0
154-155	89117.0
156-157	93015.0
158-159	97041.0
160-161	98957.0
162-163	105036.0
164-165	108468.0
166-167	112942.0
168-169	116171.0
170-171	119192.0
172-173	124884.0
174-175	135373.0
176-177	429428.0
178-179	4481779.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.59906615595496
#Duplication Level	Percentage of total
1	93.55255783280252
2	5.5782874325287
3	0.6181212376916891
4	0.12651627768802007
5	0.05547437786626921
6	0.020686913888408823
7	0.010414197571372024
8	0.0125823056891538
9	0.003166988508637592
>10	0.02051360041987956
>50	0.0016788353453609966
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3732911623083211E-5	2.7465823246166423E-5	1.3732911623083211E-5	1.3732911623083211E-5	0.0	0.0
2	2.1972658596933138E-4	2.7465823246166423E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
3	2.4719240921549783E-4	2.7465823246166423E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
4	2.6092532083858103E-4	5.4931646492332846E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
5	2.7465823246166423E-4	5.4931646492332846E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
6	2.883911440847474E-4	6.866455811541606E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
7	2.883911440847474E-4	8.239746973849927E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
8	2.883911440847474E-4	8.239746973849927E-5	4.1198734869249634E-5	1.3732911623083211E-5	0.0	0.0
9	3.295898789539971E-4	8.239746973849927E-5	5.4931646492332846E-5	1.3732911623083211E-5	0.0	0.0
10-14	3.707886138232468E-4	9.338379903696585E-5	1.0437012833543241E-4	1.3732911623083211E-5	2.7465823246166425E-6	0.0
15-19	3.982544370694131E-4	1.318359515815988E-4	1.235962046077489E-4	2.7465823246166423E-5	3.8452152544632996E-5	0.0
20-24	4.0100101939402974E-4	1.428222808800654E-4	1.483154455292987E-4	6.317139346618277E-5	6.042481114156612E-5	0.0
25-29	4.3945317193866277E-4	1.7852785110008176E-4	1.7852785110008176E-4	8.239746973849927E-5	6.866455811541606E-5	0.0
30-34	6.701660872064608E-4	2.032470920216315E-4	2.1972658596933138E-4	1.1260987530928234E-4	8.239746973849927E-5	0.0
35-39	0.0013348390097636881	2.2796633294318134E-4	2.2247316829394803E-4	1.483154455292987E-4	1.4556886320468205E-4	0.0
40-44	0.0018869020570116337	2.719116501370476E-4	2.6367190316319765E-4	1.867675980739317E-4	2.3071291526779794E-4	3.5705570220016344E-5
45-49	0.00230712915267798	2.883911440847474E-4	3.186035496555305E-4	2.0599367434624818E-4	3.3508304360323036E-4	5.218506416771621E-5
50-54	0.002603760043736577	2.966308910585974E-4	3.295898789539971E-4	2.1698000364471474E-4	4.4494633658789606E-4	7.690430508926599E-5
55-59	0.0028070071357582083	3.1311038500629726E-4	3.4881595522631356E-4	2.2521975061856467E-4	5.548096295725617E-4	8.239746973849927E-5
60-64	0.0030569461272983234	3.158569673309139E-4	3.872681077709466E-4	2.444458268908812E-4	6.207276053633612E-4	8.239746973849927E-5
65-69	0.003213501319801472	3.405762082524636E-4	4.3945317193866277E-4	2.828979794355142E-4	7.168579867249437E-4	9.338379903696585E-5
70-74	0.005872193010030381	3.790283607970967E-4	4.559326658863627E-4	3.405762082524636E-4	7.937622918142096E-4	9.613038136158248E-5
75-79	0.05837036756275289	3.872681077709466E-4	4.806519068079124E-4	3.8452152544632987E-4	9.118653317727254E-4	9.613038136158248E-5
80-84	0.08195801656656063	4.092407663678797E-4	4.806519068079124E-4	4.14733931017113E-4	0.0010986329298466571	9.613038136158248E-5
85-89	0.08215851707625763	4.1198734869249637E-4	4.806519068079124E-4	4.4494633658789606E-4	0.001474914708319137	9.613038136158248E-5
90-94	0.08222718163437304	4.2297367799096295E-4	4.971314007556121E-4	4.8614507145714576E-4	0.0014913942022668368	9.613038136158248E-5
95-99	0.08235352442130539	4.257202603155796E-4	5.328369709756285E-4	5.410767179494784E-4	0.0015106202785391534	9.613038136158248E-5
100-104	0.08250458644915933	4.4219975426327944E-4	5.383301356248619E-4	5.630493765464117E-4	0.0015847780013038023	9.613038136158248E-5
105-109	0.08259522366587167	4.6966557750944583E-4	5.740357058448782E-4	5.767822881694949E-4	0.0016397096477961354	9.613038136158248E-5
110-114	0.08274353911140098	5.081177300540788E-4	6.015015290910447E-4	5.877686174679614E-4	0.0016699220533669186	9.613038136158248E-5
115-119	0.0828973477215795	5.245972240017786E-4	6.152344407141279E-4	6.042481114156614E-4	0.0017742921817023509	9.613038136158248E-5
120-124	0.08346039709812592	5.438233002740951E-4	6.619263402326107E-4	6.23474187687978E-4	0.0018759157277131668	9.613038136158248E-5
125-129	0.08441071458244327	5.575562118971784E-4	7.003784927772438E-4	6.729126695310773E-4	0.0019500734504778162	9.613038136158248E-5
130-134	0.08539673763698065	5.712891235202615E-4	7.250977336987935E-4	7.14111404400327E-4	0.0020352175025409322	1.0711671066004906E-4
135-139	0.08647065132590574	5.767822881694949E-4	7.470703922957268E-4	7.443238099711101E-4	0.002120361554604048	1.0986329298466569E-4
140-144	0.087665414637114	5.905151997925781E-4	7.553101392695767E-4	7.690430508926598E-4	0.0021945192773686973	1.2084962228313227E-4
145-149	0.08851410857542052	6.042481114156614E-4	7.662964685680433E-4	7.690430508926598E-4	0.0022796633294318133	1.3458253390621547E-4
150-154	0.08926117896771626	6.454468462849109E-4	7.937622918142096E-4	7.855225448403595E-4	0.002329101811274913	1.4556886320468205E-4
155-159	0.09008790024742588	6.454468462849109E-4	8.157349504111427E-4	8.322144443588426E-4	0.0024169924456626454	1.5106202785391534E-4
160-164	0.09101899165547092	6.564331755833776E-4	8.267212797096093E-4	8.37707609008076E-4	0.0025241091563226944	1.5106202785391534E-4
165-167	0.0920288184234883	6.59179757907994E-4	8.468628834234648E-4	8.377076090080759E-4	0.002526855738647311	1.647949394769985E-4
>>END_MODULE
