##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479172
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3669984
Total Bases	618.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29501790743502	39.0	39.0	39.0	37.0	39.0
2	39.99967820023193	41.0	40.0	41.0	38.0	41.0
3	40.00678177343553	41.0	40.0	41.0	38.0	41.0
4	40.00311636236016	41.0	40.0	41.0	38.0	41.0
5	39.99489180334301	41.0	40.0	41.0	38.0	41.0
6	39.97109742167813	41.0	40.0	41.0	38.0	41.0
7	39.97410724406428	41.0	40.0	41.0	38.0	41.0
8	39.9290528242085	41.0	40.0	41.0	38.0	41.0
9	39.89721753555329	41.0	40.0	41.0	38.0	41.0
10-14	39.75716711571495	40.6	39.8	41.0	37.8	41.0
15-19	39.69996986362884	40.6	39.6	41.0	37.6	41.0
20-24	39.74232143791363	41.0	40.0	41.0	37.6	41.0
25-29	39.54818685858031	40.0	39.0	41.0	36.8	41.0
30-34	39.23562958312625	40.0	38.4	41.0	35.6	41.0
35-39	38.80384601131776	40.0	37.8	41.0	35.0	41.0
40-44	38.28649400106376	39.4	36.4	41.0	34.2	41.0
45-49	37.81480387925397	38.8	35.4	40.6	34.0	41.0
50-54	37.1089690854238	37.2	35.0	39.8	33.8	41.0
55-59	36.35153008841455	36.0	35.0	39.0	33.0	40.6
60-64	35.37728458761673	34.8	34.2	37.0	32.4	39.6
65-69	35.072571542546235	35.0	33.8	36.0	32.4	39.0
70-74	35.03507895402269	35.0	34.0	36.0	32.6	39.0
75-79	34.895661452475	35.0	34.0	35.4	32.0	39.0
80-84	34.892495117144925	35.0	34.0	35.0	32.0	39.6
85-89	34.713680659098245	34.8	33.4	35.8	30.8	40.0
90-94	39.46427549712731	40.6	39.6	40.6	37.0	41.0
95-99	39.56229199441143	40.4	39.0	41.0	36.6	41.0
100-104	39.33916651984735	40.0	39.0	41.0	36.0	41.0
105-109	39.379250406570044	40.0	39.0	41.0	35.8	41.0
110-114	39.22307398988839	40.0	38.4	41.0	35.0	41.0
115-119	38.97815450546854	40.0	38.0	41.0	35.0	41.0
120-124	38.66603511999746	40.0	37.4	41.0	34.8	41.0
125-129	37.9994659513882	39.2	36.2	40.4	33.8	40.8
130-134	38.054682940100875	39.0	35.4	40.8	34.0	41.0
135-139	37.585807557036176	38.2	35.0	40.8	34.0	41.0
140-144	36.93492812972899	36.6	35.0	39.6	34.0	41.0
145-149	36.247166560092886	35.6	35.0	38.4	33.4	40.6
150-154	35.59935717950756	35.0	35.0	36.8	33.0	39.2
155-159	35.084031564349345	35.0	35.0	36.0	33.0	37.4
160-164	34.70034544575762	35.0	34.2	35.0	33.0	36.4
165-169	34.453255132171066	35.0	34.0	35.0	33.0	36.0
170-174	34.30868085658384	35.0	34.0	35.0	32.8	35.6
175-178	33.66291929293367	34.75	33.25	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	50.0
31	808.0
32	5942.0
33	28448.0
34	96963.0
35	267159.0
36	683663.0
37	1283768.0
38	1126575.0
39	175923.0
40	683.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.295842161709697	28.006225640220773	28.538217060346856	22.15971513772267
2	21.9209675028556	28.592249993460463	29.15061755037624	20.336164953307698
3	20.630852886552095	27.514888348287077	29.641573369257195	22.212685395903634
4	21.071481510546093	27.002624534602877	29.947187780655177	21.978706174195857
5	21.729985743807056	27.620256655069888	28.627645243140027	22.02211235798303
6	22.115546007830005	27.415569114197773	28.19232999380924	22.276554884162984
7	22.005463784038295	27.946688595917585	27.9519747225056	22.09587289753852
8	21.819059701622677	28.27197611760705	28.148569585044513	21.760394595725757
9	22.23911602884372	27.92657951642296	27.69066568137627	22.14363877335705
10-14	21.989109489305676	28.130482312729427	27.868840845082705	22.011567352882192
15-19	22.077889167909177	27.98617105687654	27.85751654503126	22.07842323018302
20-24	21.95387772807729	27.997811434600262	28.029500946053172	22.01880989126928
25-29	21.9259593502315	28.052710856505097	27.907881887223486	22.11344790603992
30-34	21.994635398955417	28.015588078858112	27.865919851421694	22.123856670764777
35-39	21.859833721345925	28.061708170934807	27.925920113003215	22.152537994716052
40-44	21.921343526293303	28.061315798651982	27.88006705206344	22.137273622991273
45-49	22.219263081255942	27.898617541656854	27.693286946210122	22.188832430877085
50-54	22.117584163854666	27.9615823938197	27.798584407997417	22.122249034328213
55-59	22.04814516902526	27.981914907530932	27.801843277790855	22.168096645652952
60-64	22.053115217941006	27.947560534323856	27.79110208654861	22.208222161186534
65-69	21.980390105242968	27.952279900947797	27.85912418146782	22.208205812341415
70-74	21.92703837400926	28.02047093393323	27.849761742830488	22.202728949227026
75-79	21.954776914558753	27.994726952488076	27.796126631614744	22.254369501338424
80-84	21.87743052830748	28.043190379031625	27.84654646995736	22.232832622703533
85-89	21.81369183080907	28.033190335434703	27.841772607183028	22.311345226573195
90-94	21.40687319331425	27.73084221567975	28.94145213997984	21.92083245102616
95-99	21.866294443896386	27.98748713400107	28.000309482383074	22.145908939719465
100-104	21.94314049698848	27.988545226609997	27.931847685327938	22.13646659107359
105-109	21.862205618308455	28.02111771197322	28.03041585979863	22.086260809919693
110-114	21.928432809410022	27.89757044819734	27.988418914355318	22.18557782803732
115-119	21.922001179137748	27.95655051350995	27.95269465994197	22.168753647410337
120-124	21.86693915446071	28.037159454150817	27.94918455797173	22.146716833416743
125-129	21.939650342096716	27.970193170474666	27.932960485696302	22.15719600173232
130-134	21.974585384860294	27.99374108880683	27.911961779817307	22.119711746515573
135-139	21.970103237995964	28.036005553488998	27.902140061071673	22.091751147443368
140-144	21.970813952903427	28.055027285712953	27.94844041088228	22.02571835050134
145-149	21.894630361557642	28.111755500447817	28.02348855888618	21.970125579108363
150-154	21.772599493940508	28.274355976347582	28.080965130602543	21.872079399109364
155-159	21.785734819310413	28.283924564011166	28.089198749456866	21.841141867221552
160-164	21.665686071719705	28.46461414276912	28.17916538843522	21.690534397075957
165-169	21.524701444898927	28.597364587339243	28.279457827732802	21.598476140029035
170-174	21.51949525860247	28.739670824169277	28.255940036660487	21.484893880567764
175-178	21.39612013794331	28.97143199033026	28.286352599183655	21.346095272542772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	2.5
11	6.5
12	25.5
13	57.5
14	116.0
15	237.0
16	399.5
17	671.5
18	1165.5
19	1982.5
20	3118.5
21	4699.5
22	6903.0
23	9654.5
24	13302.0
25	17913.0
26	23109.0
27	28995.0
28	35526.0
29	43030.0
30	51419.5
31	60141.0
32	69274.5
33	78826.0
34	89232.5
35	99866.5
36	109981.5
37	120476.5
38	130604.5
39	139947.0
40	148368.5
41	154759.5
42	159049.5
43	160991.5
44	160562.5
45	157435.0
46	151538.5
47	143499.5
48	134171.0
49	124179.5
50	113599.5
51	103151.5
52	92746.0
53	83044.0
54	74784.5
55	67670.0
56	61207.5
57	55262.5
58	50575.5
59	46287.0
60	42293.5
61	38422.5
62	34615.5
63	31113.5
64	27540.5
65	23929.5
66	20092.0
67	16555.5
68	13474.0
69	10668.0
70	8220.5
71	6151.0
72	4515.5
73	3229.5
74	2209.5
75	1459.5
76	913.5
77	572.0
78	361.0
79	203.0
80	113.5
81	68.0
82	36.5
83	19.5
84	10.5
85	3.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	487.0
92-93	1058.0
94-95	1741.0
96-97	2296.0
98-99	2989.0
100-101	3594.0
102-103	4235.0
104-105	4859.0
106-107	5498.0
108-109	6440.0
110-111	6856.0
112-113	7456.0
114-115	8038.0
116-117	8543.0
118-119	9166.0
120-121	9787.0
122-123	10701.0
124-125	11420.0
126-127	12076.0
128-129	13033.0
130-131	13839.0
132-133	16173.0
134-135	40644.0
136-137	42644.0
138-139	42516.0
140-141	43704.0
142-143	45811.0
144-145	46542.0
146-147	46698.0
148-149	48940.0
150-151	51847.0
152-153	54327.0
154-155	49054.0
156-157	51764.0
158-159	53983.0
160-161	55925.0
162-163	60883.0
164-165	64007.0
166-167	67439.0
168-169	69666.0
170-171	73947.0
172-173	78813.0
174-175	86327.0
176-177	466816.0
178-179	1867402.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.23398928015035
#Duplication Level	Percentage of total
1	96.5874021336654
2	3.0996762009261887
3	0.2455112364575822
4	0.042693943997280805
5	0.013721672570313759
6	0.004279345132166996
7	0.0021685692540968395
8	0.0021977163644259353
9	0.0011135568051170582
>10	0.0012356248274437466
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.7248075195968156E-5	0.0	0.0	2.7248075195968156E-5	0.0	0.0
2	2.7248075195968155E-4	0.0	0.0	5.449615039193631E-5	0.0	0.0
3	2.997288271556497E-4	0.0	0.0	5.449615039193631E-5	0.0	0.0
4	3.269769023516179E-4	0.0	0.0	5.449615039193631E-5	0.0	0.0
5	3.269769023516179E-4	0.0	0.0	5.449615039193631E-5	0.0	0.0
6	3.269769023516179E-4	0.0	0.0	5.449615039193631E-5	0.0	0.0
7	3.814730527435542E-4	2.7248075195968156E-5	5.449615039193631E-5	5.449615039193631E-5	2.7248075195968156E-5	0.0
8	3.814730527435542E-4	2.7248075195968156E-5	5.449615039193631E-5	5.449615039193631E-5	2.7248075195968156E-5	0.0
9	4.0872112793952233E-4	2.7248075195968156E-5	8.174422558790447E-5	5.449615039193631E-5	2.7248075195968156E-5	0.0
10-14	4.305195880962969E-4	2.7248075195968156E-5	9.264345566629173E-5	8.71938406270981E-5	4.359692031354905E-5	0.0
15-19	5.340622738409759E-4	4.904653535274268E-5	1.1444191582306625E-4	1.198915308622599E-4	5.449615039193631E-5	0.0
20-24	5.940080392721058E-4	8.174422558790447E-5	1.580388361366153E-4	1.4168999101903443E-4	9.809307070548536E-5	0.0
25-29	6.158064994288803E-4	8.174422558790447E-5	2.1253498652855162E-4	1.907365263717771E-4	1.3624037597984078E-4	0.0
30-34	7.956437957222703E-4	8.174422558790447E-5	2.452326767637134E-4	2.288838316461325E-4	1.7983729629338983E-4	0.0
35-39	0.0013515045297200206	9.264345566629173E-5	2.670311369204879E-4	2.670311369204879E-4	2.942792121164561E-4	0.0
40-44	0.0017602256576595432	1.0899230078387262E-4	2.7248075195968155E-4	3.051784421948434E-4	4.1417074297871604E-4	0.0
45-49	0.0021798460156774524	1.4713960605822803E-4	2.7248075195968155E-4	3.814730527435542E-4	6.158064994288804E-4	5.994576543112995E-5
50-54	0.0024632259977155216	1.7983729629338983E-4	2.7248075195968155E-4	4.0872112793952233E-4	6.812018798992039E-4	8.174422558790447E-5
55-59	0.002735706749675203	1.907365263717771E-4	2.833799820380688E-4	4.468684332138778E-4	7.302484152519465E-4	8.174422558790447E-5
60-64	0.0028882959707726245	1.907365263717771E-4	3.160776722732306E-4	4.795661234490396E-4	8.555895611534001E-4	1.0354268574467898E-4
65-69	0.00310628057234037	2.288838316461325E-4	3.269769023516179E-4	4.904653535274268E-4	9.318841717021111E-4	1.0899230078387262E-4
70-74	0.005967328467917026	2.8882959707726246E-4	3.269769023516179E-4	5.231630437625885E-4	0.0010681245476819518	1.525892210974217E-4
75-79	0.06166784378351513	2.997288271556497E-4	3.3787613243000514E-4	5.994576543112995E-4	0.0012370626138969542	1.6348845117580894E-4
80-84	0.08603307262374986	3.269769023516179E-4	3.5422497754758605E-4	6.594034197424294E-4	0.0013678533748376013	1.6348845117580894E-4
85-89	0.08619656107492568	3.5422497754758605E-4	3.814730527435542E-4	6.812018798992039E-4	0.0016021868215229276	1.6348845117580894E-4
90-94	0.08628920453059195	3.705738226651669E-4	4.305195880962969E-4	6.812018798992039E-4	0.001640334126797283	1.6348845117580894E-4
95-99	0.08637639837121906	3.814730527435542E-4	4.359692031354905E-4	6.866514949383976E-4	0.0016948302771892195	1.6348845117580894E-4
100-104	0.08640364644641503	4.0872112793952233E-4	4.632172783314586E-4	7.302484152519465E-4	0.001727527967424381	1.6348845117580894E-4
105-109	0.08653443720735568	4.414188181746841E-4	4.632172783314586E-4	7.356980302911403E-4	0.0017983729629338983	1.6348845117580894E-4
110-114	0.08673607296380584	5.122638136842012E-4	5.068141986450076E-4	7.356980302911403E-4	0.0018528691133258344	1.6348845117580894E-4
115-119	0.08687776295482488	5.395118888801695E-4	5.449615039193631E-4	7.574964904479148E-4	0.0018910164186001902	1.6348845117580894E-4
120-124	0.08741727484370504	5.449615039193631E-4	5.449615039193631E-4	8.228918709182383E-4	0.0019727606441880944	1.6348845117580894E-4
125-129	0.08845270170115183	5.667599640761376E-4	5.449615039193631E-4	9.1553532658453E-4	0.002059954484815193	1.6348845117580894E-4
130-134	0.08968431470000959	6.103568843896866E-4	5.722095791153313E-4	9.863803220940472E-4	0.002212543705912615	1.6348845117580894E-4
135-139	0.09088867962367138	6.267057295072676E-4	5.994576543112994E-4	0.0010081787822508218	0.0022997375465397124	1.6348845117580894E-4
140-144	0.09238187414441043	6.267057295072676E-4	6.158064994288803E-4	0.0010136283972900155	0.0023433344668532616	1.6348845117580894E-4
145-149	0.09326471178075979	6.430545746248486E-4	6.485041896640422E-4	0.0010354268574467899	0.0023923810022060043	1.6348845117580894E-4
150-154	0.09414754941710918	6.812018798992039E-4	6.812018798992039E-4	0.0010408764724859835	0.002495923687950683	1.907365263717771E-4
155-159	0.09518842588959517	7.030003400559785E-4	6.812018798992039E-4	0.0010899230078387262	0.0025340709932250385	2.1798460156774525E-4
160-164	0.09642548850349211	7.138995701343658E-4	6.975507250167849E-4	0.001138969543191469	0.002566768683460201	2.1798460156774525E-4
165-167	0.09762077073542193	7.629461054871085E-4	7.084499550951721E-4	0.001153501849962652	0.0026339806022769217	2.1798460156774525E-4
>>END_MODULE
