##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479175
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2617777
Total Bases	424.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.146102590098394	40.0	39.0	41.0	36.0	41.0
2	39.292945120993885	40.0	39.0	41.0	36.0	41.0
3	39.374861953481904	40.0	39.0	41.0	37.0	41.0
4	39.37004832726394	40.0	39.0	41.0	37.0	41.0
5	39.343146494143696	40.0	39.0	40.0	37.0	41.0
6	39.30351172005866	40.0	39.0	40.0	37.0	41.0
7	39.28060105960133	40.0	39.0	40.0	37.0	41.0
8	39.23637804136869	40.0	39.0	40.0	36.0	41.0
9	39.20322968686791	40.0	39.0	40.0	36.0	41.0
10-14	39.36607594917366	40.0	39.0	41.0	37.0	41.0
15-19	39.288807564586286	40.0	39.0	40.8	36.2	41.0
20-24	39.12309436594485	40.0	38.6	40.0	36.0	41.0
25-29	38.86204034950265	40.0	38.0	40.0	35.0	41.0
30-34	38.495910843437	40.0	37.4	40.0	34.6	41.0
35-39	37.97370761527816	39.4	36.2	40.0	34.0	41.0
40-44	37.4272493799128	38.4	35.2	40.0	33.0	41.0
45-49	36.63090904993054	36.8	34.6	39.8	32.8	40.2
50-54	35.81417905344879	35.6	34.0	38.6	32.0	40.0
55-59	35.08334300438884	35.0	33.4	36.8	31.6	39.4
60-64	34.320062786096756	34.6	32.8	35.8	31.0	38.8
65-69	34.50042665971929	35.0	33.0	35.6	31.8	38.0
70-74	34.387108145575425	34.2	33.0	35.0	31.0	38.0
75-79	34.3841382974944	34.0	33.0	35.0	31.0	39.0
80-84	34.46519447607646	34.0	33.0	35.0	31.0	39.8
85-89	35.204883685661535	35.2	33.4	37.4	30.2	40.4
90-94	38.92440463453952	40.0	38.0	41.0	35.0	41.0
95-99	38.82814538237535	40.0	38.0	41.0	35.0	41.0
100-104	38.809557917174715	40.0	38.0	41.0	35.0	41.0
105-109	38.640881122574754	40.0	37.8	41.0	34.2	41.0
110-114	38.39336627572183	40.0	37.0	41.0	34.0	41.0
115-119	38.090426819661566	39.4	36.0	41.0	34.0	41.0
120-124	37.32500278050177	38.2	35.0	39.8	33.0	40.6
125-129	37.310830743620926	38.0	35.0	40.0	33.0	41.0
130-134	36.98028487762246	37.2	35.0	40.0	33.0	41.0
135-139	36.31890620315452	36.0	35.0	39.0	33.0	40.8
140-144	35.627337914565594	35.0	34.6	37.2	33.0	39.4
145-149	35.01712170566741	35.0	34.0	36.2	32.2	38.0
150-154	34.55766756267838	35.0	34.0	35.2	32.0	36.6
155-159	34.27998963119042	35.0	34.0	35.0	32.0	36.0
160-164	34.07728329870887	35.0	34.0	35.0	31.6	35.2
165-169	33.94413763917589	35.0	34.0	35.0	31.0	35.0
170-174	33.861276867476406	35.0	34.0	35.0	31.0	35.0
175-176	32.370987751259314	33.5	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	81.0
31	1262.0
32	9431.0
33	45591.0
34	154908.0
35	399402.0
36	769155.0
37	895915.0
38	327511.0
39	14490.0
40	31.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.427426018335407	27.02033060875697	28.06106096890606	22.491182404001563
2	22.046530319427514	27.146544568158404	28.391570404965737	22.415354707448344
3	21.95817290777633	27.29289011248857	28.181239272863962	22.567697706871133
4	22.294909001034082	27.47735960702535	27.6925421836925	22.535189208248067
5	22.349344501078587	27.608577812395787	27.714163582306668	22.327914104218962
6	22.045155106794812	27.660492089280332	27.748582098475154	22.5457707054497
7	22.374174729169063	27.587949622905235	27.480377434747115	22.557498213178587
8	22.581029629338175	27.66324251454574	27.457877428062055	22.29785042805403
9	22.229624601331587	27.383768747299715	27.586154206412537	22.80045244495616
10-14	22.390234156690962	27.497078628164278	27.58483247427111	22.52785474087365
15-19	22.326378450112443	27.279390108477536	27.724019272841037	22.67021216856898
20-24	22.412405640358212	27.29088077403079	27.595513292385103	22.70120029322589
25-29	22.4703097322652	27.328462279254495	27.47259984330216	22.728628145178142
30-34	22.354646709784674	27.257638828670277	27.56043009011081	22.827284371434235
35-39	22.382937889667453	27.28312610279638	27.549382548628092	22.784553458908075
40-44	22.531101770700865	27.258998761162623	27.356768739277637	22.853130728858876
45-49	22.68452966008946	27.202913005958873	27.28254545746257	22.8300118764891
50-54	22.66127328645641	27.18996308700092	27.34696652923454	22.801797097308135
55-59	22.64182166777384	27.25257346213982	27.285548005043974	22.820056865042364
60-64	22.507967638190724	27.18381283050466	27.404213575105903	22.904005956198713
65-69	22.52826730466346	27.23269399952708	27.41930271371473	22.819735982094734
70-74	22.465840291208917	27.253085346842	27.418294224450747	22.862780137498344
75-79	22.413872533833096	27.20730604631334	27.408201691740743	22.970619728112823
80-84	22.338426840788962	27.252176178490377	27.507484403751732	22.901912576968932
85-89	22.290752802855245	27.096165945380374	27.710549829110732	22.90253142265365
90-94	21.968045498223788	27.73681814322228	27.785555977010905	22.509580381543024
95-99	22.206158899899265	27.71147263841333	27.487942678781074	22.59442578290633
100-104	22.218739039996173	27.630899035146555	27.509158610963684	22.641203313893588
105-109	22.29969789932812	27.542057232081298	27.486661653985113	22.671583214605473
110-114	22.381401796518922	27.600239040134046	27.316474562619586	22.701884600727446
115-119	22.386590783610554	27.55875431499046	27.313361740148284	22.741293161250706
120-124	22.42828651648798	27.555236812088978	27.295238693538625	22.721237977884414
125-129	22.51287427068788	27.52861061730779	27.247392894262624	22.711122217741707
130-134	22.590414796791887	27.522714609366627	27.158263278093003	22.728607315748476
135-139	22.65855442755866	27.521731768745468	27.181722603273506	22.637991200422373
140-144	22.63777209205567	27.54521999958049	27.19430014480461	22.622707763559234
145-149	22.557177641339855	27.59295970053406	27.23665068757588	22.613211970550203
150-154	22.560746801857068	27.6501662127644	27.264791382306942	22.524295603071593
155-159	22.490398596989344	27.749140207553268	27.296606930072038	22.46385426538535
160-164	22.38901054630091	27.752010293949684	27.39254240260757	22.466436757141828
165-169	22.392667118582334	27.848352415582372	27.489639503482554	22.269340962352736
170-174	22.274736418296904	27.991766578777177	27.552632461881455	22.18086454104447
175-176	22.460698030626613	27.62752902397229	27.826130852546214	22.085642092854883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	3.0
17	5.5
18	12.0
19	24.0
20	48.0
21	106.5
22	230.5
23	454.5
24	898.5
25	1650.0
26	2800.0
27	4578.5
28	6943.0
29	10080.0
30	14353.5
31	19688.0
32	26251.0
33	33809.5
34	42856.5
35	53485.0
36	64805.0
37	77018.0
38	89824.5
39	102262.0
40	114161.5
41	125019.5
42	133743.5
43	139801.0
44	142980.5
45	143135.0
46	140668.5
47	134766.0
48	126640.5
49	117411.5
50	106461.5
51	95233.5
52	83995.5
53	73169.5
54	63323.0
55	54321.5
56	46110.0
57	38996.0
58	33075.5
59	27776.5
60	23361.5
61	19708.0
62	16351.0
63	13569.0
64	11318.0
65	9384.5
66	7639.0
67	6093.5
68	4830.5
69	3760.0
70	2822.0
71	2068.5
72	1486.0
73	1072.5
74	719.0
75	438.0
76	274.5
77	169.5
78	115.0
79	68.5
80	38.0
81	21.5
82	9.0
83	3.5
84	1.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	710.0
92-93	1555.0
94-95	2510.0
96-97	3409.0
98-99	4308.0
100-101	5263.0
102-103	6243.0
104-105	7225.0
106-107	7857.0
108-109	8709.0
110-111	9215.0
112-113	10031.0
114-115	10949.0
116-117	11627.0
118-119	12355.0
120-121	13261.0
122-123	14231.0
124-125	15068.0
126-127	16169.0
128-129	17424.0
130-131	18562.0
132-133	34411.0
134-135	45070.0
136-137	45572.0
138-139	46285.0
140-141	46550.0
142-143	49093.0
144-145	52092.0
146-147	50534.0
148-149	45146.0
150-151	50080.0
152-153	50381.0
154-155	51466.0
156-157	50609.0
158-159	49657.0
160-161	56672.0
162-163	59841.0
164-165	58920.0
166-167	61450.0
168-169	67829.0
170-171	64136.0
172-173	74765.0
174-175	357269.0
176-177	953268.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.60783682991882
#Duplication Level	Percentage of total
1	89.53845955936444
2	9.591154312473162
3	0.7179054464866081
4	0.10175801168814037
5	0.032438876337978796
6	0.008823090583359392
7	0.005623750579983847
8	0.0
9	0.0011692952634047935
>10	0.002667657222926009
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	3.8200350908423447E-5	0.0	0.0	0.0	0.0	0.0
4	7.640070181684689E-5	0.0	0.0	0.0	0.0	0.0
5	7.640070181684689E-5	0.0	0.0	0.0	0.0	0.0
6	7.640070181684689E-5	0.0	0.0	3.8200350908423447E-5	0.0	0.0
7	7.640070181684689E-5	0.0	0.0	3.8200350908423447E-5	0.0	0.0
8	7.640070181684689E-5	0.0	0.0	3.8200350908423447E-5	0.0	0.0
9	7.640070181684689E-5	0.0	0.0	3.8200350908423447E-5	0.0	0.0
10-14	7.640070181684689E-5	0.0	0.0	3.8200350908423447E-5	0.0	0.0
15-19	7.640070181684689E-5	0.0	0.0	6.112056145347751E-5	0.0	0.0
20-24	7.640070181684689E-5	0.0	6.112056145347751E-5	8.404077199853158E-5	0.0	0.0
25-29	7.640070181684689E-5	1.5280140363369378E-5	1.1460105272527034E-4	1.5280140363369379E-4	3.8200350908423447E-5	0.0
30-34	9.168084218021627E-5	3.8200350908423447E-5	1.2224112290695502E-4	1.8336168436043258E-4	3.8200350908423447E-5	0.0
35-39	1.5280140363369379E-4	7.640070181684689E-5	1.5280140363369379E-4	1.9100175454211723E-4	4.584042109010814E-5	1.5280140363369378E-5
40-44	1.8336168436043258E-4	7.640070181684689E-5	1.5280140363369379E-4	2.368421756322254E-4	7.640070181684689E-5	3.8200350908423447E-5
45-49	2.062818949054866E-4	7.640070181684689E-5	1.5280140363369379E-4	3.0560280726738757E-4	7.640070181684689E-5	3.8200350908423447E-5
50-54	3.514432283574957E-4	7.640070181684689E-5	1.9100175454211723E-4	3.0560280726738757E-4	8.404077199853158E-5	3.8200350908423447E-5
55-59	4.125637898109732E-4	1.1460105272527034E-4	1.9100175454211723E-4	3.0560280726738757E-4	1.1460105272527034E-4	3.8200350908423447E-5
60-64	4.3548400035602725E-4	1.5280140363369379E-4	1.9100175454211723E-4	3.0560280726738757E-4	1.1460105272527034E-4	3.8200350908423447E-5
65-69	5.500850530812977E-4	1.5280140363369379E-4	1.9100175454211723E-4	3.0560280726738757E-4	1.1460105272527034E-4	3.8200350908423447E-5
70-74	0.0017495760716057934	1.9100175454211723E-4	1.9100175454211723E-4	3.1324287744907226E-4	1.7572161417874784E-4	3.8200350908423447E-5
75-79	0.00887776155111761	1.9100175454211723E-4	2.1392196508717128E-4	3.43803158175811E-4	2.979627370857029E-4	3.8200350908423447E-5
80-84	0.0104286957979996	2.062818949054866E-4	2.674024563589641E-4	3.8200350908423447E-4	3.2088294763075694E-4	3.8200350908423447E-5
85-89	0.0104286957979996	2.9796273708570283E-4	3.2088294763075694E-4	3.8200350908423447E-4	3.8200350908423447E-4	3.8200350908423447E-5
90-94	0.0104286957979996	3.590832985391804E-4	3.43803158175811E-4	3.972836494476039E-4	3.8200350908423447E-4	3.8200350908423447E-5
95-99	0.010466896148908025	4.431240705377119E-4	3.43803158175811E-4	4.3548400035602725E-4	3.8964357926591915E-4	3.8200350908423447E-5
100-104	0.010520376640179819	4.5840421090108136E-4	3.43803158175811E-4	4.660442810827661E-4	4.202038599926579E-4	3.8200350908423447E-5
105-109	0.010543296850724871	4.5840421090108136E-4	3.43803158175811E-4	5.65365193444667E-4	4.202038599926579E-4	3.8200350908423447E-5
110-114	0.010543296850724871	4.5840421090108136E-4	3.43803158175811E-4	5.730052636263517E-4	4.431240705377119E-4	3.8200350908423447E-5
115-119	0.010573857131451609	4.889644916278201E-4	3.43803158175811E-4	5.959254741714058E-4	4.5840421090108136E-4	3.8200350908423447E-5
120-124	0.010634977692905088	5.118847021728741E-4	3.43803158175811E-4	6.112056145347751E-4	4.5840421090108136E-4	3.8200350908423447E-5
125-129	0.010711378394721933	5.348049127179282E-4	3.43803158175811E-4	6.341258250798291E-4	4.5840421090108136E-4	3.8200350908423447E-5
130-134	0.010856539728173943	5.348049127179282E-4	3.43803158175811E-4	6.723261759882525E-4	4.660442810827661E-4	3.8200350908423447E-5
135-139	0.010986420921262582	5.730052636263517E-4	3.43803158175811E-4	6.952463865333066E-4	5.195247723545588E-4	3.8200350908423447E-5
140-144	0.011123942184532908	6.112056145347751E-4	3.43803158175811E-4	7.258066672600455E-4	5.348049127179282E-4	3.8200350908423447E-5
145-149	0.01119270281616807	6.112056145347751E-4	3.43803158175811E-4	7.258066672600455E-4	5.348049127179282E-4	3.8200350908423447E-5
150-154	0.01133786414962008	6.264857548981445E-4	3.43803158175811E-4	7.258066672600455E-4	5.348049127179282E-4	3.8200350908423447E-5
155-159	0.011628186816524095	6.494059654431985E-4	3.43803158175811E-4	7.258066672600455E-4	5.577251232629823E-4	3.8200350908423447E-5
160-164	0.011918509483428114	6.494059654431985E-4	3.514432283574957E-4	7.258066672600455E-4	5.730052636263517E-4	3.8200350908423447E-5
165	0.012071310887061808	6.494059654431985E-4	3.8200350908423447E-4	7.258066672600455E-4	5.730052636263517E-4	3.8200350908423447E-5
>>END_MODULE
