##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479183
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6079720
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29326679518136	39.0	39.0	39.0	37.0	39.0
2	40.01026708466837	41.0	40.0	41.0	38.0	41.0
3	40.03438036620108	41.0	40.0	41.0	38.0	41.0
4	40.047909443198044	41.0	40.0	41.0	38.0	41.0
5	40.053986038830736	41.0	40.0	41.0	38.0	41.0
6	40.04565440513708	41.0	40.0	41.0	38.0	41.0
7	40.072672425703814	41.0	40.0	41.0	38.0	41.0
8	40.058286236866174	41.0	40.0	41.0	38.0	41.0
9	40.043086852684006	41.0	40.0	41.0	38.0	41.0
10-14	39.98117406722678	41.0	40.0	41.0	38.0	41.0
15-19	39.87250018092938	41.0	40.0	41.0	38.0	41.0
20-24	39.712528142743416	40.8	39.6	41.0	37.4	41.0
25-29	39.52605083786754	40.0	39.0	41.0	36.8	41.0
30-34	39.50636562210102	40.2	39.0	41.0	36.4	41.0
35-39	39.443015171751334	40.6	39.0	41.0	35.6	41.0
40-44	39.08598583487397	40.0	38.2	41.0	35.0	41.0
45-49	38.580222312869665	39.8	36.8	41.0	35.0	41.0
50-54	37.95465208924095	38.8	35.6	40.8	34.6	41.0
55-59	37.226946767285334	37.2	35.0	39.8	34.0	41.0
60-64	36.363581908377355	35.8	34.8	38.6	33.8	40.4
65-69	35.804986315159255	35.0	35.0	37.0	33.6	39.0
70-74	35.39669540702532	35.0	35.0	36.2	33.4	38.4
75-79	35.08843769120947	35.0	35.0	35.8	33.0	37.0
80-84	34.948389563993075	35.0	35.0	35.0	33.0	36.6
85-89	34.75308553683393	35.0	34.6	35.0	32.6	36.2
90-94	39.17972218826792	40.2	39.6	40.2	37.0	40.2
95-99	39.74375504669938	41.0	40.0	41.0	37.0	41.0
100-104	39.6578960129959	41.0	39.4	41.0	37.0	41.0
105-109	39.53316526949938	40.8	39.0	41.0	36.4	41.0
110-114	39.37042253928057	40.0	39.0	41.0	36.0	41.0
115-119	39.1586418516183	40.0	38.0	41.0	35.0	41.0
120-124	38.902019090833946	40.0	38.0	41.0	35.0	41.0
125-129	38.66063852342703	39.8	37.8	40.8	34.8	41.0
130-134	38.38637697946017	39.8	37.0	40.8	34.0	41.0
135-139	38.2064890859934	39.2	36.0	41.0	34.0	41.0
140-144	37.87689844213607	38.8	35.2	41.0	34.0	41.0
145-149	37.18834719559801	37.2	35.0	39.8	34.0	41.0
150-154	36.44040663744788	36.0	35.0	38.8	34.0	40.6
155-159	35.74367646974669	35.0	35.0	37.0	33.8	39.0
160-164	35.197096061059625	35.0	35.0	36.0	33.0	37.4
165-169	34.823226208222366	35.0	35.0	35.2	33.0	36.4
170-174	34.61359290382224	35.0	35.0	35.0	33.0	36.0
175-179	34.33122916440409	35.0	33.8	35.0	32.6	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	42.0
30	477.0
31	3250.0
32	15676.0
33	55589.0
34	153217.0
35	351393.0
36	766543.0
37	1574132.0
38	2301361.0
39	848480.0
40	9558.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.516816563920706	29.893860243563847	28.91417696867619	20.675146223839256
2	21.12786444112558	29.820287776410755	29.330528379596426	19.721319402867238
3	19.785253268242617	29.15005954221576	30.131486318448875	20.933200871092748
4	19.88038922845131	28.14759561295586	30.979962893027967	20.992052265564862
5	20.64090122571434	28.704331778437165	29.73011915022402	20.92464784562447
6	20.932148191035115	28.708805668682107	29.336844459942235	21.02220168034054
7	20.690245603415946	28.967205726579515	29.292039764989177	21.050508905015363
8	20.867457711868308	29.088592895725462	29.16808668820274	20.87586270420348
9	21.127042034830552	29.10028751324074	28.796539972235564	20.976130479693143
10-14	20.90367648510129	29.103656089425172	28.941013730895506	21.05165369457804
15-19	20.888550788523155	29.09501095445185	28.997947273887615	21.01849098313738
20-24	20.87097761081102	29.04253814320396	29.080549762160103	21.005934483824912
25-29	20.837400406597673	29.103504766666887	28.97178488483022	21.087309941905218
30-34	20.920177245004705	29.169971643430948	28.89847229806636	21.01137881349799
35-39	20.901074391583823	29.1727579559585	28.93070075595587	20.995466896501814
40-44	20.826439375497554	29.19557150658254	28.997055785463804	20.9809333324561
45-49	20.984048607501663	29.134986479640514	28.867954445270506	21.01301046758732
50-54	20.952504391649615	29.189357404617315	28.896610370214415	20.961527833518648
55-59	20.89307073352062	29.15588875803491	28.950356266407006	21.00068424203746
60-64	20.908426703861362	29.18807774042226	28.924147822597096	20.979347733119287
65-69	20.92656240747929	29.153003756751954	28.942648674610016	20.97778516115874
70-74	20.82623870836157	29.207578638489927	28.96221865480647	21.00396399834203
75-79	20.890060726480826	29.15970801286901	28.928723691222626	21.021507569427538
80-84	20.880303698196627	29.191778568749875	28.915558611251836	21.012359121801662
85-89	20.857884902594197	29.191143671090114	28.888323804385728	21.06264762192996
90-94	20.43286551097192	29.05064371706344	29.934453190986936	20.5820375809777
95-99	20.674165459670064	28.894612662779863	29.385850618394183	21.045371259155893
100-104	20.75742189537706	29.053224115558645	29.09007965382586	21.099274335238437
105-109	20.78976365885333	29.0289536586188	29.12068248096263	21.060600201565236
110-114	20.778023608609587	28.951732096282264	29.201998119254753	21.068246175853396
115-119	20.76200986751496	28.98249364307144	29.108802658629372	21.146693830784233
120-124	20.803011199970552	29.034536937648447	29.073038666545077	21.089413195835924
125-129	20.80071376336814	29.023382778979023	29.11107976046688	21.064823697185957
130-134	20.794945565684365	29.049853961993726	29.122030789403443	21.03316968291847
135-139	20.921334655200553	29.040180523102237	29.01809621711645	21.02038860458076
140-144	20.89266242276397	29.107485743414983	29.091349740973772	20.90850209284728
145-149	20.797119007438212	29.117591110797463	29.144147249799946	20.941142631964382
150-154	20.79875743709703	29.20438924772613	29.149300051427936	20.847553263748903
155-159	20.771949722864505	29.206596844192983	29.196072446911348	20.82538098603116
160-164	20.662223317716307	29.287817060661393	29.24833095821868	20.801628663403616
165-169	20.684682064250616	29.352369574066415	29.213029291948324	20.749919069734645
170-174	20.63815684714612	29.448336710925656	29.248708477304632	20.664797964623595
175-179	20.5648639161531	29.5128164911823	29.291157013840635	20.63116257882397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	4.0
9	10.5
10	17.0
11	31.5
12	59.0
13	126.0
14	233.0
15	410.0
16	734.0
17	1332.0
18	2381.5
19	4023.0
20	6621.0
21	10494.0
22	15798.0
23	22686.0
24	31390.0
25	42212.0
26	54931.5
27	68834.5
28	84234.5
29	101246.0
30	118544.5
31	137335.5
32	156353.5
33	174593.0
34	193106.0
35	211230.5
36	227991.5
37	243223.0
38	256047.0
39	265926.0
40	273222.0
41	275823.0
42	273990.0
43	267923.0
44	258438.0
45	245595.0
46	229769.5
47	212722.0
48	194871.5
49	176622.0
50	158418.5
51	140859.0
52	124556.5
53	109495.0
54	95466.0
55	83192.5
56	72586.5
57	63628.5
58	55945.0
59	49302.5
60	43767.5
61	38756.5
62	34083.5
63	29913.0
64	26235.5
65	22710.0
66	19373.5
67	16275.0
68	13264.5
69	10692.5
70	8440.0
71	6422.0
72	4813.5
73	3574.5
74	2548.0
75	1722.0
76	1198.0
77	791.5
78	469.5
79	289.5
80	178.5
81	93.5
82	54.5
83	27.0
84	13.0
85	8.5
86	4.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	813.0
92-93	1933.0
94-95	2721.0
96-97	3575.0
98-99	4106.0
100-101	4880.0
102-103	5616.0
104-105	6291.0
106-107	7180.0
108-109	7718.0
110-111	8118.0
112-113	8982.0
114-115	9083.0
116-117	10590.0
118-119	10364.0
120-121	11489.0
122-123	11018.0
124-125	11346.0
126-127	13866.0
128-129	12523.0
130-131	14618.0
132-133	14058.0
134-135	60677.0
136-137	63536.0
138-139	51913.0
140-141	54436.0
142-143	50133.0
144-145	54097.0
146-147	53025.0
148-149	53994.0
150-151	54554.0
152-153	54276.0
154-155	58167.0
156-157	52189.0
158-159	51384.0
160-161	53154.0
162-163	54638.0
164-165	56584.0
166-167	59048.0
168-169	70232.0
170-171	62807.0
172-173	64747.0
174-175	69661.0
176-177	74965.0
178-179	4620615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.87963509619735
#Duplication Level	Percentage of total
1	94.05169881984177
2	5.180692285037804
3	0.6065902277125473
4	0.09937192880497209
5	0.035118166410048356
6	0.009372304268660623
7	0.005249462706553613
8	0.005292317160522285
9	0.0021341595890972355
>10	0.004480328468020517
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	3.289625180106979E-5	0.0	0.0	1.6448125900534893E-5	0.0
2	4.934437770160468E-5	3.289625180106979E-5	0.0	0.0	1.6448125900534893E-5	0.0
3	4.934437770160468E-5	6.579250360213957E-5	0.0	0.0	1.6448125900534893E-5	0.0
4	4.934437770160468E-5	6.579250360213957E-5	0.0	1.6448125900534893E-5	3.289625180106979E-5	0.0
5	4.934437770160468E-5	8.224062950267446E-5	0.0	3.289625180106979E-5	6.579250360213957E-5	0.0
6	4.934437770160468E-5	8.224062950267446E-5	0.0	4.934437770160468E-5	6.579250360213957E-5	0.0
7	4.934437770160468E-5	8.224062950267446E-5	0.0	4.934437770160468E-5	6.579250360213957E-5	0.0
8	6.579250360213957E-5	8.224062950267446E-5	0.0	4.934437770160468E-5	6.579250360213957E-5	0.0
9	6.579250360213957E-5	8.224062950267446E-5	0.0	8.224062950267446E-5	6.579250360213957E-5	0.0
10-14	1.1513688130374426E-4	9.21095050429954E-5	2.631700144085583E-5	8.881987986288843E-5	6.579250360213957E-5	0.0
15-19	2.0066713598652572E-4	1.1842650648385123E-4	3.6185876981176765E-5	1.250057568440652E-4	8.224062950267447E-5	0.0
20-24	2.138256367069536E-4	1.9079826044620475E-4	4.934437770160467E-5	1.6119163382524195E-4	1.1513688130374426E-4	0.0
25-29	2.4014263814780944E-4	2.533011388682374E-4	5.263400288171166E-5	2.4672188850802343E-4	2.0724638634673966E-4	0.0
30-34	3.684380201719816E-4	2.6645963958866526E-4	8.224062950267446E-5	2.7632851512898623E-4	3.6185876981176767E-4	0.0
35-39	7.664826669649261E-4	2.9606626620962807E-4	8.224062950267446E-5	3.421210187311258E-4	4.2436164823380024E-4	0.0
40-44	0.001220450941819689	2.9606626620962807E-4	1.0526800576342332E-4	3.881757712526235E-4	5.098919029165817E-4	1.6448125900534893E-5
45-49	0.0016448125900534894	3.09224766930056E-4	1.3158500720427915E-4	4.177823978735863E-4	6.02001407959577E-4	1.6448125900534893E-5
50-54	0.002134966741889429	3.12514392110163E-4	1.4474350792470706E-4	4.67126775575191E-4	7.237175396235353E-4	1.9737751080641872E-5
55-59	0.002536301013862481	3.1580401729026995E-4	1.743501345456699E-4	4.8357490147572584E-4	8.224062950267447E-4	3.289625180106979E-5
60-64	0.0028521050311527503	3.585691446316607E-4	1.9408788562631175E-4	5.362089043574375E-4	8.914884238089912E-4	3.289625180106979E-5
65-69	0.0031909364247037692	4.276512734139072E-4	2.1711526188706058E-4	5.789740316988282E-4	0.0010033356799326286	5.592362806181863E-5
70-74	0.005569335429921114	4.4409939931444207E-4	2.697492647687723E-4	6.250287842203259E-4	0.0011217621864164798	6.579250360213957E-5
75-79	0.04890685755265045	4.934437770160469E-4	2.9935589138973504E-4	6.612146612015027E-4	0.0012270301921799031	6.579250360213957E-5
80-84	0.0672136216799458	5.493674050778655E-4	3.12514392110163E-4	7.171382892633214E-4	0.0014704624555078195	6.579250360213957E-5
85-89	0.06734849631233017	6.151599086800049E-4	3.2896251801069784E-4	8.256959202068516E-4	0.0018454797260400151	6.579250360213957E-5
90-94	0.067404419940392	6.414769101208608E-4	3.684380201719816E-4	8.520129216477073E-4	0.0018685071023007642	6.579250360213957E-5
95-99	0.06748008131953445	6.645042863816096E-4	3.783068957123025E-4	9.34253551150382E-4	0.0018750863526609779	6.579250360213957E-5
100-104	0.06762811445263926	6.842420374622516E-4	3.815965208924095E-4	9.803083036718798E-4	0.0018849552282012988	6.579250360213957E-5
105-109	0.06780904383754516	6.941109130025725E-4	3.947550216128374E-4	0.0010197838058331635	0.001891534478561513	6.579250360213957E-5
110-114	0.06799984209799136	7.89510043225675E-4	4.1120314751337235E-4	0.0010197838058331635	0.0018981137289217267	6.579250360213957E-5
115-119	0.06824327436131927	8.322751705670657E-4	4.2107202305369327E-4	0.001036231931733698	0.0019211411051824757	6.579250360213957E-5
120-124	0.06874987663905574	9.375431763304889E-4	4.276512734139072E-4	0.001082286684255196	0.0020198298605856846	6.579250360213957E-5
125-129	0.06948017342903949	9.803083036718796E-4	4.276512734139072E-4	0.001108603685696052	0.0021020704900883594	6.579250360213957E-5
130-134	0.07031244859960656	0.001036231931733698	4.276512734139072E-4	0.0011349206871369077	0.002204048870671676	6.579250360213957E-5
135-139	0.07119735777305533	0.001062548933174554	4.276512734139072E-4	0.0011875546900186195	0.002250103623193173	6.579250360213957E-5
140-144	0.07205594994506326	0.0010954451849756238	4.309408985940142E-4	0.0012467679432605447	0.002299448000894778	6.579250360213957E-5
145-149	0.072970465745133	0.0011349206871369077	4.506786496746561E-4	0.0012763745698815077	0.0023981367562979873	6.579250360213957E-5
150-154	0.07373036916173772	0.0011480791878573356	4.6054752521497704E-4	0.0012928226957820426	0.0025231425131420526	6.579250360213957E-5
155-159	0.07451329995460318	0.0011809754396584055	4.934437770160469E-4	0.0013322981979433262	0.00260209351746462	6.579250360213957E-5
160-164	0.07539820912805195	0.0011842650648385123	5.098919029165817E-4	0.001375063325284717	0.0026876237721474012	6.579250360213957E-5
165-168	0.07626173573783003	0.0012007131907390471	5.098919029165817E-4	0.0013857546071200648	0.0027345009309639257	6.579250360213957E-5
>>END_MODULE
