##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479184
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6019840
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.19179297124176	39.0	39.0	39.0	37.0	39.0
2	39.941708251382096	41.0	40.0	41.0	38.0	41.0
3	40.00162246837125	41.0	40.0	41.0	38.0	41.0
4	40.01892774558792	41.0	40.0	41.0	38.0	41.0
5	40.03588351185414	41.0	40.0	41.0	38.0	41.0
6	40.02864926642569	41.0	40.0	41.0	38.0	41.0
7	40.05632491893472	41.0	40.0	41.0	38.0	41.0
8	40.04163582420795	41.0	40.0	41.0	38.0	41.0
9	40.023547968052306	41.0	40.0	41.0	38.0	41.0
10-14	39.96556333723156	41.0	40.0	41.0	38.0	41.0
15-19	39.85137365777164	41.0	40.0	41.0	38.0	41.0
20-24	39.68916775196683	40.8	39.6	41.0	37.2	41.0
25-29	39.50176735594302	40.0	39.0	41.0	36.6	41.0
30-34	39.46808440091431	40.0	39.0	41.0	36.0	41.0
35-39	39.395717892834355	40.2	39.0	41.0	35.6	41.0
40-44	39.03465351238571	40.0	37.8	41.0	35.0	41.0
45-49	38.53958048718903	39.8	36.6	41.0	35.0	41.0
50-54	37.92021880980225	38.8	35.4	40.8	34.4	41.0
55-59	37.21196972012545	37.2	35.0	39.8	34.0	41.0
60-64	36.37914642249628	35.8	34.8	38.6	33.8	40.4
65-69	35.840826168137355	35.0	35.0	37.0	33.6	39.0
70-74	35.43124631219434	35.0	35.0	36.2	33.4	38.6
75-79	35.131990451573465	35.0	35.0	36.0	33.0	37.0
80-84	34.98563164469488	35.0	35.0	35.0	33.0	37.0
85-89	34.795607458005534	35.0	34.6	35.0	32.6	37.0
90-94	39.39081063824576	40.6	39.8	40.6	36.8	40.6
95-99	39.67782098257866	41.0	39.8	41.0	37.0	41.0
100-104	39.575156861341156	41.0	39.0	41.0	36.6	41.0
105-109	39.43393586324699	40.2	39.0	41.0	36.0	41.0
110-114	39.25794328158664	40.0	38.6	41.0	35.6	41.0
115-119	39.032461282238444	40.0	38.0	41.0	35.0	41.0
120-124	38.792334067745216	40.0	38.0	41.0	35.0	41.0
125-129	38.43397865846583	39.6	37.2	40.6	34.6	41.0
130-134	38.32218839250943	39.8	36.6	41.0	34.0	41.0
135-139	38.08273975448522	39.0	35.8	41.0	34.0	41.0
140-144	37.67231861436822	38.4	35.0	40.8	34.0	41.0
145-149	36.97878369883852	36.8	35.0	39.4	34.0	41.0
150-154	36.24760540644079	35.8	35.0	38.2	34.0	40.2
155-159	35.581593267794446	35.0	35.0	36.8	33.0	39.0
160-164	35.07002936004345	35.0	35.0	36.0	33.0	37.0
165-169	34.738017697016936	35.0	35.0	35.0	33.0	36.2
170-174	34.55366379879403	35.0	34.2	35.0	33.0	36.0
175-178	34.24559428727057	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	2.0
29	46.0
30	435.0
31	3210.0
32	15533.0
33	56700.0
34	156093.0
35	361675.0
36	785981.0
37	1580761.0
38	2235319.0
39	813864.0
40	10220.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.48517900807995	29.91635990325324	28.94248019880927	20.655980889857535
2	21.110561078035296	29.862039522645116	29.325264458856047	19.702134940463534
3	19.76494391877525	29.104594142036998	30.190951918987878	20.939510020199872
4	19.916376514990432	28.144751355517755	30.969261641505426	20.969610487986394
5	20.60176017967255	28.692805788858177	29.736870082925794	20.968563948543483
6	20.89547230491176	28.73518233042739	29.387026897724855	20.982318466935997
7	20.672393286200297	28.964092068892196	29.32235740484797	21.041157240059537
8	20.812430230703807	29.101803370189238	29.21295250372103	20.872813895385924
9	21.10346786625558	29.085507256006803	28.86470404529024	20.946320832447373
10-14	20.883820832447377	29.117810440144588	28.969318121411863	21.029050605996172
15-19	20.88777442589836	29.095573968743356	29.017468902827982	20.9991827025303
20-24	20.854477859876674	29.054257256006803	29.10101265149904	20.99025223261748
25-29	20.822530166914735	29.11933871996598	28.966955932383588	21.0911751807357
30-34	20.906681905166916	29.1529808101212	28.9393804486498	21.000956836062088
35-39	20.871800579417393	29.193742690835638	28.947908914522646	20.986547815224323
40-44	20.83507535083989	29.201164815011694	28.996451732936425	20.967308101211994
45-49	20.9747169359983	29.151415984477993	28.872149425898364	21.001717653625345
50-54	20.94419453008718	29.188357165638955	28.931014777801405	20.936433526472463
55-59	20.882468637040187	29.15944609823517	28.96175978099086	20.996325483733788
60-64	20.89949566765894	29.1910682011482	28.949171406549013	20.960264724643846
65-69	20.902319662981075	29.159605570912184	28.96609876674463	20.971975999362108
70-74	20.82028093769934	29.19188882096534	28.98029847969381	21.007531761641506
75-79	20.861693998511587	29.179529688496704	28.94422110886668	21.014555204125028
80-84	20.854344965979163	29.204978869870295	28.93892528705082	21.001750877099724
85-89	20.82538406336381	29.21426150861152	28.92779874548161	21.03255568254306
90-94	20.314461960337848	29.10742715751045	29.836086329328705	20.742024552822997
95-99	20.67822167070078	29.01074079577904	29.267957417124496	21.043080116395686
100-104	20.822064596961713	29.07603006762442	29.04582416477562	21.05608117063825
105-109	20.70474294146613	29.055059477790707	29.20070954846439	21.03948803227878
110-114	20.76502532376223	28.954292268476106	29.18995032960251	21.090732078159153
115-119	20.800629036060545	29.026798911185224	29.10852574054723	21.064046312207005
120-124	20.739327693638444	29.038603393164497	29.141098040331336	21.080970872865727
125-129	20.74735731424538	29.071913651661006	29.14324213998815	21.037486894105463
130-134	20.83883643919582	29.063954588068885	29.095119437271443	21.00208953546385
135-139	20.861128349167153	29.0656291222448	29.073304400010546	20.9999381285775
140-144	20.873567286387352	29.101675526013327	29.10290969727218	20.92184749032714
145-149	20.833777286734946	29.134482284119034	29.121941025174543	20.90979940397148
150-154	20.756585869331925	29.21578284809278	29.19348766962845	20.834143612946843
155-159	20.75013132396907	29.237377555545553	29.191980692779968	20.820510427705408
160-164	20.715026991505145	29.32311754828305	29.213982909589483	20.747872550622322
165-169	20.60295035550432	29.36711963184114	29.29838415663857	20.73154585601597
170-174	20.612476649408208	29.484122641474965	29.255975376641107	20.647425332475716
175-178	20.55380524569087	29.545144717905874	29.285651951515852	20.615398084887403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	1.5
9	6.0
10	12.0
11	24.5
12	58.0
13	115.0
14	216.0
15	419.5
16	771.5
17	1340.0
18	2301.5
19	3914.0
20	6568.0
21	10493.5
22	15564.5
23	22306.5
24	31096.0
25	41916.5
26	54047.0
27	67964.0
28	83910.5
29	100536.0
30	117908.0
31	136158.5
32	154558.0
33	173254.5
34	191604.0
35	209518.5
36	226602.0
37	241482.0
38	254407.0
39	264154.5
40	270540.0
41	273059.5
42	270986.5
43	264976.5
44	255595.5
45	243155.5
46	228233.5
47	211399.0
48	193866.5
49	175341.0
50	156778.0
51	139302.0
52	122731.0
53	107610.5
54	94047.0
55	82389.0
56	71878.5
57	62806.5
58	55359.5
59	48516.5
60	42439.0
61	37489.5
62	33190.0
63	29285.0
64	25667.5
65	22365.0
66	18974.5
67	15740.0
68	13046.0
69	10522.0
70	8160.5
71	6221.0
72	4755.0
73	3518.0
74	2432.0
75	1708.0
76	1183.5
77	781.5
78	493.0
79	304.0
80	175.0
81	92.5
82	45.5
83	26.5
84	16.5
85	8.5
86	3.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1025.0
92-93	2103.0
94-95	2808.0
96-97	3611.0
98-99	4479.0
100-101	6355.0
102-103	5980.0
104-105	7216.0
106-107	7725.0
108-109	8730.0
110-111	9259.0
112-113	10040.0
114-115	11067.0
116-117	10687.0
118-119	11307.0
120-121	11675.0
122-123	13171.0
124-125	13553.0
126-127	12913.0
128-129	14413.0
130-131	14757.0
132-133	15829.0
134-135	78239.0
136-137	65833.0
138-139	58384.0
140-141	57726.0
142-143	66962.0
144-145	66701.0
146-147	61961.0
148-149	66288.0
150-151	61750.0
152-153	58521.0
154-155	63186.0
156-157	62926.0
158-159	67262.0
160-161	60398.0
162-163	59197.0
164-165	61806.0
166-167	64984.0
168-169	70325.0
170-171	69489.0
172-173	72593.0
174-175	78776.0
176-177	172904.0
178-179	4274926.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.82002456037091
#Duplication Level	Percentage of total
1	93.92638770960204
2	5.32724025732611
3	0.5812903737324862
4	0.10144813427017108
5	0.033064381777712246
6	0.012502101659877864
7	0.005252341189313196
8	0.006354847272678832
9	0.002135721416129462
>10	0.004324131753472274
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.983521156708484E-5	0.0	0.0	1.661173718902828E-5	0.0	0.0
2	8.30586859451414E-5	0.0	0.0	1.661173718902828E-5	0.0	0.0
3	8.30586859451414E-5	0.0	0.0	1.661173718902828E-5	0.0	0.0
4	8.30586859451414E-5	0.0	0.0	1.661173718902828E-5	0.0	0.0
5	1.1628216032319796E-4	0.0	0.0	1.661173718902828E-5	0.0	0.0
6	1.3289389751222624E-4	0.0	0.0	1.661173718902828E-5	0.0	0.0
7	1.4950563470125453E-4	0.0	0.0	1.661173718902828E-5	0.0	0.0
8	1.661173718902828E-4	0.0	0.0	3.322347437805656E-5	0.0	0.0
9	1.661173718902828E-4	0.0	0.0	4.983521156708484E-5	0.0	0.0
10-14	1.7940676164150544E-4	2.657877950244525E-5	6.644694875611312E-6	5.6479906442696157E-5	2.657877950244525E-5	0.0
15-19	1.9601849883053372E-4	6.312460131830747E-5	4.6512864129279184E-5	9.302572825855837E-5	5.31575590048905E-5	0.0
20-24	2.3256432064639593E-4	1.295715500744206E-4	4.983521156708484E-5	1.6279502445247715E-4	1.1295981288539231E-4	0.0
25-29	2.624654475866468E-4	1.6943971932808846E-4	5.31575590048905E-5	1.8272910907931109E-4	1.4618328726344887E-4	0.0
30-34	4.15293429725707E-4	1.8272910907931109E-4	7.641399106953009E-5	1.9269615139272807E-4	2.724324899000638E-4	0.0
35-39	8.272645120136084E-4	2.2259727833297895E-4	8.30586859451414E-5	2.2591962577078463E-4	3.355570912183713E-4	0.0
40-44	0.0012193015096746758	2.491760578354242E-4	8.970338082075272E-5	2.624654475866468E-4	4.020040399744844E-4	0.0
45-49	0.0016412396342759942	2.7575483733786947E-4	9.967042313416968E-5	2.7575483733786947E-4	4.6845098873059753E-4	0.0
50-54	0.002192749308951733	2.9901126940250905E-4	9.967042313416968E-5	3.255900489049543E-4	5.647990644269615E-4	0.0
55-59	0.0024950829257920477	3.2226770146714863E-4	1.1295981288539231E-4	3.388794386561769E-4	6.27923665745269E-4	0.0
60-64	0.002687779077184776	3.488464809695939E-4	1.3953859238783755E-4	4.15293429725707E-4	7.973633850733575E-4	6.644694875611312E-6
65-69	0.0030067244312141188	3.9203699766106743E-4	1.6279502445247715E-4	4.485169041037636E-4	9.169678928343611E-4	1.661173718902828E-5
70-74	0.005375558154369552	4.6845098873059753E-4	1.7608441420369978E-4	4.7177333616840316E-4	0.0010066712736551138	1.661173718902828E-5
75-79	0.046712204975547525	4.917074207952371E-4	1.8272910907931109E-4	5.016744631086541E-4	0.0011262757814161175	1.661173718902828E-5
80-84	0.06439041569211142	4.983521156708484E-4	1.8272910907931109E-4	5.31575590048905E-4	0.0012724590686795662	1.661173718902828E-5
85-89	0.06454988836912609	5.11641505422071E-4	1.893738039549224E-4	5.714437593025729E-4	0.0016644960663406336	1.661173718902828E-5
90-94	0.0646694928768871	5.481873272379333E-4	2.12630236019562E-4	6.146342759940464E-4	0.0017342653625345525	1.661173718902828E-5
95-99	0.0648488996385286	6.07989581118435E-4	2.3256432064639593E-4	6.312460131830747E-4	0.0017641664894748033	1.661173718902828E-5
100-104	0.06497847118860302	6.179566234318521E-4	2.425313629598129E-4	6.445354029342973E-4	0.001787422921539443	1.661173718902828E-5
105-109	0.06517448968743356	6.478577503721029E-4	2.790771847756751E-4	6.810812247501595E-4	0.0018571922177333619	1.661173718902828E-5
110-114	0.0652641930682543	6.578247926855199E-4	2.8239953221348077E-4	6.976929619391878E-4	0.0018804486497980013	1.661173718902828E-5
115-119	0.06537050818626408	6.976929619391878E-4	2.9568892196470337E-4	7.209493940038274E-4	0.001980119072932171	1.661173718902828E-5
120-124	0.06581238039549224	7.010153093769935E-4	2.9901126940250905E-4	7.475281735062726E-4	0.0021196576653200088	1.661173718902828E-5
125-129	0.06655990856899852	7.275940888794387E-4	3.1562300659153733E-4	8.006857325111631E-4	0.0021894269615139275	1.661173718902828E-5
130-134	0.06730079204762918	7.408834786306613E-4	3.322347437805656E-4	8.139751222623857E-4	0.0023057091218371254	1.661173718902828E-5
135-139	0.06811808951732937	7.641399106953009E-4	3.322347437805656E-4	8.272645120136084E-4	0.0024685041462896027	1.661173718902828E-5
140-144	0.06879252604720391	7.674622581331066E-4	3.455241335317882E-4	8.737773761428875E-4	0.002571496916861578	1.661173718902828E-5
145-149	0.06950018605145651	7.807516478843292E-4	3.488464809695939E-4	9.435466723368063E-4	0.002667844992557942	1.661173718902828E-5
150-154	0.07016797788645546	8.006857325111631E-4	3.488464809695939E-4	9.601584095258346E-4	0.0027210025515628324	1.661173718902828E-5
155-159	0.07102846587284713	8.272645120136084E-4	3.5216882840739957E-4	9.967042313416968E-4	0.0027774824580055284	1.661173718902828E-5
160-164	0.07206836062088029	8.40553901764831E-4	3.721029130342335E-4	0.0010764405698490327	0.002847251754199447	1.661173718902828E-5
165-167	0.07272064816783613	8.471985966404423E-4	3.9314444680700264E-4	0.0010853001630165143	0.002907054008079949	1.661173718902828E-5
>>END_MODULE
