##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479185
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3769660
Total Bases	647.2 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23799971350201	39.0	39.0	39.0	37.0	39.0
2	39.98185512751813	41.0	40.0	41.0	38.0	41.0
3	40.033331653252546	41.0	40.0	41.0	38.0	41.0
4	40.048892738337145	41.0	40.0	41.0	38.0	41.0
5	40.05394677504072	41.0	40.0	41.0	38.0	41.0
6	40.04830515218879	41.0	40.0	41.0	38.0	41.0
7	40.0715014086151	41.0	40.0	41.0	38.0	41.0
8	40.04486134028002	41.0	40.0	41.0	38.0	41.0
9	40.03950780707013	41.0	40.0	41.0	38.0	41.0
10-14	39.98271435620188	41.0	40.0	41.0	38.0	41.0
15-19	39.867945066663836	41.0	40.0	41.0	38.0	41.0
20-24	39.69800417013736	40.8	39.6	41.0	37.2	41.0
25-29	39.49863998344678	40.0	39.0	41.0	36.8	41.0
30-34	39.47536515229491	40.2	39.0	41.0	36.0	41.0
35-39	39.41106609084109	40.4	39.0	41.0	35.6	41.0
40-44	39.04222152660982	40.0	37.8	41.0	35.0	41.0
45-49	38.536383069030094	39.8	36.6	41.0	35.0	41.0
50-54	37.921412275908175	38.8	35.4	40.8	34.6	41.0
55-59	37.20993728877405	37.2	35.0	39.8	34.0	41.0
60-64	36.36502273414579	35.8	34.8	38.6	33.8	40.4
65-69	35.83656653385186	35.0	35.0	37.0	33.6	39.0
70-74	35.44388730018092	35.0	35.0	36.2	33.4	38.6
75-79	35.13825941862131	35.0	35.0	36.0	33.0	37.0
80-84	34.993840770785695	35.0	35.0	35.0	33.0	37.0
85-89	34.786636248361916	35.0	34.6	35.0	32.6	37.0
90-94	39.162982700377206	40.2	39.6	40.2	36.8	40.2
95-99	39.724816704068076	41.0	40.0	41.0	37.0	41.0
100-104	39.6255379914095	41.0	39.0	41.0	37.0	41.0
105-109	39.499755712679715	40.6	39.0	41.0	36.0	41.0
110-114	39.32757250537156	40.0	38.8	41.0	35.8	41.0
115-119	39.10775327042503	40.0	38.0	41.0	35.0	41.0
120-124	38.84121411733257	40.0	38.0	41.0	35.0	41.0
125-129	38.59203315630252	39.8	37.6	40.8	34.8	41.0
130-134	38.33605007517501	39.8	36.8	40.8	34.0	41.0
135-139	38.15919360109139	39.0	36.0	41.0	34.0	41.0
140-144	37.82846681128308	38.8	35.2	41.0	34.0	41.0
145-149	37.153176559783574	37.0	35.0	39.6	34.0	41.0
150-154	36.415760080106146	36.0	35.0	38.8	34.0	40.6
155-159	35.71894193311475	35.0	35.0	37.0	33.6	39.0
160-164	35.17222568934328	35.0	35.0	36.0	33.0	37.4
165-169	34.80415072062246	35.0	35.0	35.2	33.0	36.4
170-174	34.58934594453668	35.0	34.6	35.0	33.0	36.0
175-179	34.30472243185239	35.0	33.8	35.0	32.4	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	23.0
30	211.0
31	1687.0
32	8326.0
33	31913.0
34	92534.0
35	219297.0
36	488277.0
37	997140.0
38	1420406.0
39	503808.0
40	6038.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.523813818752885	29.863648180472506	28.929532106343807	20.6830058944308
2	21.123363910803626	29.809080925070162	29.356891602956235	19.710663561169973
3	19.76459415437997	29.11060944488362	30.153382533172753	20.971413867563655
4	19.910389796427264	28.14927606203212	30.937564661003908	21.002769480536706
5	20.69433317593629	28.614649597045887	29.709443291967975	20.981573935049845
6	20.925600717306068	28.688157552670535	29.362435869547916	21.02380586047548
7	20.70369741568205	28.908840585092555	29.2761150873023	21.11134691192309
8	20.878302021933013	29.027551556373783	29.188043484027737	20.906102937665466
9	21.11110816360096	29.06941209552055	28.857032199190378	20.96244754168811
10-14	20.913732272937082	29.094401086570144	28.93508698397203	21.056779656520746
15-19	20.92193990970008	29.077083874938324	28.97508528620618	21.025890929155413
20-24	20.870189884498867	29.040183995373585	29.069868370091733	21.01975775003581
25-29	20.843996540802088	29.093833396115297	28.938524959810696	21.12364510327191
30-34	20.89767246913515	29.15020983324756	28.924327392921377	21.027790304695916
35-39	20.904956945719242	29.136134293278438	28.92335648307805	21.035552277924268
40-44	20.860072261158834	29.181804194542742	28.96846399940578	20.989659544892643
45-49	20.986380734602058	29.100003713862787	28.884795976294946	21.028819575240206
50-54	20.96460688762382	29.159064743239444	28.91234753266873	20.96398083646801
55-59	20.918560294562376	29.131099356440632	28.930534849296752	21.01980549970024
60-64	20.915944674055485	29.157754280226868	28.917297581214218	21.009003464503433
65-69	20.93422749001236	29.141195757707592	28.923160178902073	21.00141657337797
70-74	20.853588917833438	29.162810439137747	28.968554193216367	21.01504644981245
75-79	20.882328910299602	29.156019375752724	28.915822647135286	21.04582906681239
80-84	20.903651788224934	29.186154719523778	28.90120594430267	21.00898754794862
85-89	20.876323063618468	29.174615217287503	28.901619774727692	21.047441944366334
90-94	20.45578431428737	29.050230976908253	29.90500367538039	20.58898103342399
95-99	20.719833913822846	28.89178149355053	29.351096112350316	21.037288480276313
100-104	20.78676528917788	29.047358930251356	29.06920878913768	21.09666699143309
105-109	20.812919649229517	29.031824654274587	29.09657883164261	21.05867686485329
110-114	20.78761875919528	28.94056301937763	29.190636533370046	21.081181688057047
115-119	20.76839955136505	28.969284175630584	29.103037358497332	21.159278914507038
120-124	20.833224301763632	29.03335890258806	29.057931554702236	21.075485240946072
125-129	20.79433656198982	29.023032812424677	29.097365517831342	21.08526510775416
130-134	20.816301039391256	29.050979822545674	29.085952683988015	21.046766454075055
135-139	20.948424672657083	29.03778131641922	29.000675761569905	21.013118249353788
140-144	20.895831464091494	29.10751382187886	29.086192281992414	20.91046243203723
145-149	20.808202209710895	29.104563989743408	29.147504967222602	20.939728833323095
150-154	20.817393136884633	29.194231590379633	29.132293637762835	20.856081634972902
155-159	20.798647007169528	29.212817553677283	29.17915989843821	20.80937554071497
160-164	20.674097345780158	29.2998220765715	29.235617802671026	20.790462774977318
165-169	20.68052930056711	29.351519720959278	29.215144992104463	20.75280598636915
170-174	20.6420145338138	29.445732725047293	29.247045732725045	20.665207008413862
175-179	20.540564647682693	29.538925949241516	29.28585967915588	20.63464972391991
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	4.5
10	6.0
11	11.5
12	33.0
13	70.0
14	127.5
15	221.5
16	450.5
17	829.0
18	1437.0
19	2433.0
20	3974.0
21	6293.0
22	9457.0
23	13664.5
24	19065.5
25	25830.0
26	33510.5
27	42151.0
28	51918.5
29	62477.0
30	73217.0
31	84515.5
32	96234.5
33	107924.5
34	119684.0
35	131009.0
36	141292.0
37	150624.0
38	159046.0
39	165607.0
40	169359.0
41	170563.5
42	170156.0
43	166854.5
44	160684.0
45	152629.5
46	143036.5
47	132944.5
48	121813.5
49	110182.0
50	98773.0
51	87290.0
52	76982.5
53	67847.5
54	59360.5
55	51660.5
56	45159.5
57	39666.0
58	34871.0
59	30635.0
60	26925.0
61	23926.5
62	21251.5
63	18587.5
64	16124.0
65	13995.5
66	11935.5
67	10054.0
68	8311.0
69	6555.5
70	5105.5
71	3966.5
72	3002.0
73	2187.0
74	1513.5
75	1059.0
76	731.0
77	474.0
78	315.0
79	190.0
80	111.5
81	66.5
82	37.5
83	19.0
84	11.5
85	7.5
86	3.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	608.0
92-93	1325.0
94-95	1789.0
96-97	2328.0
98-99	2967.0
100-101	4364.0
102-103	3783.0
104-105	4870.0
106-107	4988.0
108-109	5028.0
110-111	5622.0
112-113	5777.0
114-115	6218.0
116-117	6585.0
118-119	6599.0
120-121	7603.0
122-123	7398.0
124-125	7770.0
126-127	8064.0
128-129	9415.0
130-131	8995.0
132-133	10411.0
134-135	39713.0
136-137	40904.0
138-139	40175.0
140-141	38741.0
142-143	35470.0
144-145	38094.0
146-147	37100.0
148-149	35424.0
150-151	42070.0
152-153	36197.0
154-155	36564.0
156-157	43340.0
158-159	33294.0
160-161	36579.0
162-163	35480.0
164-165	36691.0
166-167	41632.0
168-169	37392.0
170-171	40957.0
172-173	43810.0
174-175	43698.0
176-177	47285.0
178-179	2786543.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.98495823549437
#Duplication Level	Percentage of total
1	96.0958016774517
2	3.5633143690233973
3	0.2802221177817872
4	0.04472634905097015
5	0.009485637155044767
6	0.005339453304773364
7	0.0
8	0.0
9	0.0011103962323106076
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.6527591347760807E-5	2.6527591347760807E-5	0.0
2	2.6527591347760807E-5	0.0	0.0	2.6527591347760807E-5	2.6527591347760807E-5	0.0
3	2.6527591347760807E-5	5.305518269552161E-5	0.0	2.6527591347760807E-5	2.6527591347760807E-5	0.0
4	5.305518269552161E-5	5.305518269552161E-5	0.0	5.305518269552161E-5	2.6527591347760807E-5	0.0
5	5.305518269552161E-5	5.305518269552161E-5	0.0	5.305518269552161E-5	2.6527591347760807E-5	0.0
6	5.305518269552161E-5	5.305518269552161E-5	0.0	5.305518269552161E-5	5.305518269552161E-5	0.0
7	5.305518269552161E-5	5.305518269552161E-5	0.0	5.305518269552161E-5	7.958277404328242E-5	0.0
8	5.305518269552161E-5	5.305518269552161E-5	0.0	7.958277404328242E-5	7.958277404328242E-5	0.0
9	5.305518269552161E-5	5.305518269552161E-5	0.0	7.958277404328242E-5	1.0611036539104323E-4	0.0
10-14	7.427725577373025E-5	6.366621923462593E-5	4.244414615641729E-5	1.3794347500835618E-4	1.2733243846925184E-4	0.0
15-19	7.958277404328242E-5	7.958277404328242E-5	7.958277404328242E-5	1.8569313943432564E-4	1.3263795673880402E-4	0.0
20-24	7.958277404328242E-5	9.019381058238674E-5	1.5386002981701265E-4	1.8569313943432564E-4	1.5386002981701265E-4	0.0
25-29	7.958277404328242E-5	1.3263795673880402E-4	1.5916554808656483E-4	2.1222073078208643E-4	1.8569313943432564E-4	0.0
30-34	1.5386002981701265E-4	1.5916554808656483E-4	1.5916554808656483E-4	2.1222073078208643E-4	2.5997039520805584E-4	0.0
35-39	5.25246308685664E-4	2.0691521251253432E-4	1.5916554808656483E-4	2.3874832212984726E-4	3.28942132712234E-4	0.0
40-44	0.0010345760625626713	2.546648769385037E-4	1.64471066356117E-4	2.3874832212984726E-4	4.2444146156417285E-4	0.0
45-49	0.001581044444326544	2.705814317471602E-4	2.1222073078208648E-4	2.3874832212984726E-4	5.62384936572529E-4	0.0
50-54	0.002021402460699373	3.60775242329547E-4	2.3874832212984726E-4	2.6527591347760805E-4	7.26856002928646E-4	0.0
55-59	0.002360955629950712	4.191359432946207E-4	2.5997039520805584E-4	2.918035048253689E-4	8.064387769719286E-4	2.6527591347760803E-5
60-64	0.002695203280932498	4.509690529119337E-4	3.28942132712234E-4	3.077200596340253E-4	8.701049962065544E-4	2.6527591347760803E-5
65-69	0.0029657847126796583	4.509690529119337E-4	3.713862788686513E-4	3.1833109617312967E-4	9.284656971716282E-4	2.6527591347760803E-5
70-74	0.005581405219568874	4.509690529119337E-4	3.713862788686513E-4	3.1833109617312967E-4	0.0010133539894844625	2.6527591347760803E-5
75-79	0.05068892154730135	4.668856077205902E-4	4.1383042502506854E-4	3.7669179713820344E-4	0.0010504926173713278	2.6527591347760803E-5
80-84	0.06931659619169898	4.93413199068351E-4	4.350524981032772E-4	4.2974697983372506E-4	0.0012574078298838623	2.6527591347760803E-5
85-89	0.06953942795902017	5.517739000334247E-4	4.509690529119337E-4	5.305518269552161E-4	0.001575738926056992	2.6527591347760803E-5
90-94	0.06963492728787211	5.836070096507378E-4	4.8280216252924674E-4	5.358573452247682E-4	0.001618183072213409	2.6527591347760803E-5
95-99	0.06978348179941958	6.101346009984985E-4	5.305518269552161E-4	6.048290827289463E-4	0.0016287941087525133	2.6527591347760803E-5
100-104	0.0699585639023148	6.101346009984985E-4	5.305518269552161E-4	6.313566740767071E-4	0.0016712382549089306	2.6527591347760803E-5
105-109	0.07019200670617509	6.313566740767071E-4	5.305518269552161E-4	6.89717375041781E-4	0.0016924603279871394	2.6527591347760803E-5
110-114	0.07034056121772256	6.366621923462593E-4	5.358573452247682E-4	7.427725577373026E-4	0.0017030713645262436	2.6527591347760803E-5
115-119	0.07037769984560942	6.472732288853636E-4	5.570794183029769E-4	7.746056673546156E-4	0.001750821028952213	2.6527591347760803E-5
120-124	0.07066419783216524	6.738008202331245E-4	5.62384936572529E-4	8.435774048587936E-4	0.0018887645039605695	2.6527591347760803E-5
125-129	0.07152369179183268	7.26856002928646E-4	5.836070096507378E-4	8.488829231283457E-4	0.001957736241464748	2.6527591347760803E-5
130-134	0.0725529623361258	8.064387769719286E-4	6.048290827289463E-4	8.488829231283457E-4	0.002000180387621165	2.6527591347760803E-5
135-139	0.07355039977080162	8.754105144761065E-4	6.207456375376028E-4	8.754105144761065E-4	0.0021275128260904164	2.6527591347760803E-5
140-144	0.0743727551025822	8.754105144761065E-4	6.366621923462593E-4	8.86021551015211E-4	0.00218587352705549	2.6527591347760803E-5
145-149	0.07512613869685861	8.754105144761065E-4	6.419677106158114E-4	9.019381058238674E-4	0.002249539746290116	3.183310961731297E-5
150-154	0.07585299469978726	9.019381058238674E-4	6.631897836940202E-4	9.231601789020761E-4	0.0023079004472551902	5.3055182695521607E-5
155-159	0.07678676591522843	9.284656971716282E-4	6.844118567722288E-4	9.709098433280456E-4	0.0024352328857244416	5.3055182695521607E-5
160-164	0.0776887040210523	9.602988067889413E-4	6.89717375041781E-4	0.0010717146904495364	0.0025678708424632457	5.3055182695521607E-5
165-168	0.07837576863695929	9.815208798671499E-4	6.89717375041781E-4	0.0011406864279537147	0.002659391032613021	5.305518269552161E-5
>>END_MODULE
