##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479186
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6056555
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.234951552491474	39.0	39.0	39.0	37.0	39.0
2	39.97675427697759	41.0	40.0	41.0	38.0	41.0
3	40.0229686678318	41.0	40.0	41.0	38.0	41.0
4	40.0373664566738	41.0	40.0	41.0	38.0	41.0
5	40.05668767145679	41.0	40.0	41.0	38.0	41.0
6	40.04194513217497	41.0	40.0	41.0	38.0	41.0
7	40.06256213309381	41.0	40.0	41.0	38.0	41.0
8	40.05542391673154	41.0	40.0	41.0	38.0	41.0
9	40.04291911821159	41.0	40.0	41.0	38.0	41.0
10-14	39.98351429154032	41.0	40.0	41.0	38.0	41.0
15-19	39.871278276181755	41.0	40.0	41.0	38.0	41.0
20-24	39.70990848758081	40.8	39.6	41.0	37.4	41.0
25-29	39.52694368333154	40.0	39.0	41.0	36.8	41.0
30-34	39.503254837114504	40.2	39.0	41.0	36.4	41.0
35-39	39.43190632298394	40.4	39.0	41.0	35.6	41.0
40-44	39.07186223851678	40.0	37.8	41.0	35.0	41.0
45-49	38.56964492190693	39.8	36.8	41.0	35.0	41.0
50-54	37.947112013347514	38.8	35.6	40.8	34.6	41.0
55-59	37.22224548443794	37.2	35.0	39.8	34.0	41.0
60-64	36.37312046204484	35.8	34.8	38.6	33.8	40.4
65-69	35.82476196451613	35.0	35.0	37.0	33.6	39.0
70-74	35.42262537036318	35.0	35.0	36.2	33.4	38.4
75-79	35.1298571217466	35.0	35.0	36.0	33.0	37.0
80-84	34.98308493855005	35.0	35.0	35.0	33.0	37.0
85-89	34.77965704926315	35.0	34.6	35.0	32.6	36.2
90-94	39.409638084961976	40.6	39.8	40.6	36.8	40.6
95-99	39.70343661040483	41.0	39.8	41.0	37.0	41.0
100-104	39.60043973770223	41.0	39.0	41.0	36.8	41.0
105-109	39.465039681433026	40.4	39.0	41.0	36.0	41.0
110-114	39.289183616960685	40.0	38.6	41.0	35.6	41.0
115-119	39.061185594932454	40.0	38.0	41.0	35.0	41.0
120-124	38.822460915988884	40.0	38.0	41.0	35.0	41.0
125-129	38.45462422523916	39.6	37.6	40.6	34.6	41.0
130-134	38.348583744855404	39.8	36.6	41.0	34.0	41.0
135-139	38.110877771063194	39.0	36.0	41.0	34.0	41.0
140-144	37.6885094636297	38.4	35.0	40.8	34.0	41.0
145-149	36.99504816673858	36.8	35.0	39.4	34.0	41.0
150-154	36.25568558936696	35.8	35.0	38.2	34.0	40.2
155-159	35.59532792279327	35.0	35.0	36.8	33.2	39.0
160-164	35.080577855675585	35.0	35.0	36.0	33.0	37.0
165-169	34.74903660409755	35.0	35.0	35.0	33.0	36.2
170-174	34.557877447658434	35.0	34.4	35.0	33.0	36.0
175-178	34.24176329749724	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	36.0
30	424.0
31	3076.0
32	14975.0
33	55407.0
34	153132.0
35	356083.0
36	780668.0
37	1586343.0
38	2271859.0
39	824750.0
40	9802.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.542470100576978	29.86904271487669	28.91206304574135	20.67642413880498
2	21.13024648500674	29.887105788686803	29.29454120370409	19.688106522602368
3	19.72796416444662	29.126343276004263	30.192675539147256	20.953017020401862
4	19.885017142583532	28.155395270083407	30.989564199450015	20.970023387883046
5	20.613913355034338	28.675790115007626	29.74436787909959	20.96592865085845
6	20.946478649991622	28.728592409381243	29.354856019634923	20.970072920992212
7	20.69493301059761	28.951474889603084	29.300963996859604	21.052628102939707
8	20.802667523039087	29.116733852825575	29.217467685837907	20.86313093829743
9	21.107345677534507	29.130107792301068	28.81354169160521	20.949004838559212
10-14	20.894482094193812	29.111529574155604	28.97045597703645	21.023532354614133
15-19	20.896347841305825	29.082021049920293	29.017472804259185	21.004158304514693
20-24	20.861314724294587	29.059457067590404	29.09067283298839	20.98855537512662
25-29	20.83317991828688	29.109531738752477	28.97598717422693	21.08130116873371
30-34	20.910583656880853	29.172131682119623	28.919037307512273	20.998247353487255
35-39	20.883125803365115	29.1712830148492	28.96663532321592	20.978955858569766
40-44	20.825667396729656	29.22373197304408	29.002966207687376	20.947634422538886
45-49	20.969930926079265	29.161855213070798	28.883396584361904	20.984817276488034
50-54	20.93857316576833	29.189963601420278	28.93416802125961	20.93729521155178
55-59	20.882171465461802	29.179753176517014	28.940927639557472	20.99714771846371
60-64	20.90093460721483	29.178746003297253	28.949117113606665	20.971202275881257
65-69	20.899696279485617	29.166134873702955	28.97882046807137	20.95534837874006
70-74	20.814664442079696	29.201732668158716	28.984127115166956	20.999475774594632
75-79	20.870435420796145	29.17854126644602	28.938312291393377	21.012711021364456
80-84	20.851992593149077	29.22584208349466	28.93215697702737	20.990008346328896
85-89	20.831291055723923	29.209169238948547	28.90599028655729	21.053549418770242
90-94	20.302353802943657	29.105808406804286	29.822724449406323	20.769113340845735
95-99	20.675772494430163	29.00487283889841	29.278250683337152	21.041103983334274
100-104	20.815125494208043	29.05062761514593	29.060453305543128	21.0737935851029
105-109	20.70362125639838	29.054960432324922	29.212760078994833	21.028658232281863
110-114	20.768627838958878	28.9545288573777	29.19468505657501	21.082158247088415
115-119	20.793376435044834	29.007700826627055	29.10596076135202	21.0929619769761
120-124	20.751912173139488	29.0358594590855	29.135256257539094	21.076972110235918
125-129	20.752023935037833	29.05824920804525	29.13532188961131	21.054404967305608
130-134	20.84995419729031	29.07447940305416	29.085425834359523	20.990140565296006
135-139	20.85682235417713	29.067071190447365	29.065875283858826	21.01023117151668
140-144	20.875381108233938	29.1098882402784	29.10845058278329	20.90628006870437
145-149	20.83580576107646	29.136112923218683	29.12510430111512	20.902977014589734
150-154	20.747936581219186	29.19763032424235	29.21032805430758	20.844105040230872
155-159	20.746383140596055	29.239164809916495	29.19381828263914	20.820633766848303
160-164	20.720263636031508	29.326625709943265	29.213717554327417	20.739393099697807
165-169	20.60548603054373	29.378006073784192	29.27695499413318	20.739552901538897
170-174	20.60517748173511	29.481335626782567	29.257206586189017	20.656280305293304
175-178	20.58616448584078	29.51388898586248	29.274151651291437	20.62579487700531
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	5.5
9	5.5
10	14.5
11	27.0
12	46.5
13	104.5
14	220.0
15	395.0
16	733.0
17	1330.5
18	2349.5
19	4025.0
20	6672.0
21	10487.5
22	15634.0
23	22597.0
24	31271.5
25	41829.5
26	54627.0
27	68818.0
28	84394.0
29	101024.5
30	118344.0
31	136595.5
32	155168.5
33	173996.5
34	192748.0
35	210737.5
36	227217.0
37	242708.5
38	255734.5
39	265601.0
40	272322.0
41	274672.5
42	273034.0
43	267454.0
44	258318.5
45	245001.5
46	229254.5
47	212864.5
48	194997.5
49	176004.5
50	157667.5
51	140284.0
52	123648.0
53	108567.5
54	95011.5
55	83029.5
56	72617.0
57	63334.5
58	55302.0
59	48794.5
60	43119.5
61	37999.0
62	33700.0
63	29606.0
64	25416.0
65	21822.5
66	18742.5
67	15822.0
68	12977.0
69	10377.5
70	8226.0
71	6304.0
72	4660.0
73	3432.0
74	2479.0
75	1723.0
76	1181.0
77	775.0
78	475.5
79	293.0
80	199.5
81	104.5
82	42.5
83	26.0
84	12.0
85	7.0
86	4.5
87	2.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	901.0
92-93	1902.0
94-95	2791.0
96-97	3654.0
98-99	4836.0
100-101	5082.0
102-103	5745.0
104-105	6830.0
106-107	8251.0
108-109	8007.0
110-111	8447.0
112-113	9112.0
114-115	9747.0
116-117	9985.0
118-119	10346.0
120-121	11407.0
122-123	11682.0
124-125	12167.0
126-127	12890.0
128-129	13351.0
130-131	14297.0
132-133	15037.0
134-135	71561.0
136-137	59432.0
138-139	58499.0
140-141	58060.0
142-143	63431.0
144-145	59803.0
146-147	63748.0
148-149	61887.0
150-151	60670.0
152-153	61220.0
154-155	61203.0
156-157	60610.0
158-159	59680.0
160-161	61608.0
162-163	59886.0
164-165	65925.0
166-167	63516.0
168-169	67039.0
170-171	70392.0
172-173	82236.0
174-175	77439.0
176-177	178510.0
178-179	4343733.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.85766068956164
#Duplication Level	Percentage of total
1	94.00627338167057
2	5.237271477458347
3	0.5812239007128397
4	0.11065121838461386
5	0.03615558905119661
6	0.014581437136763784
7	0.0063005489185497185
8	0.0021173952011810447
9	0.0032020486423641717
>10	0.0022230028235803306
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.651103638949865E-5	0.0	0.0	1.651103638949865E-5	0.0	0.0
2	1.651103638949865E-5	0.0	0.0	1.651103638949865E-5	0.0	0.0
3	3.30220727789973E-5	3.30220727789973E-5	0.0	1.651103638949865E-5	0.0	0.0
4	3.30220727789973E-5	6.60441455579946E-5	0.0	3.30220727789973E-5	0.0	0.0
5	3.30220727789973E-5	6.60441455579946E-5	3.30220727789973E-5	3.30220727789973E-5	1.651103638949865E-5	0.0
6	3.30220727789973E-5	8.255518194749326E-5	3.30220727789973E-5	4.9533109168495954E-5	3.30220727789973E-5	0.0
7	6.60441455579946E-5	8.255518194749326E-5	3.30220727789973E-5	6.60441455579946E-5	3.30220727789973E-5	0.0
8	6.60441455579946E-5	8.255518194749326E-5	3.30220727789973E-5	8.255518194749326E-5	6.60441455579946E-5	0.0
9	8.255518194749326E-5	8.255518194749326E-5	3.30220727789973E-5	9.906621833699191E-5	8.255518194749326E-5	0.0
10-14	1.0897284017069112E-4	9.246180378119245E-5	4.2928694612696496E-5	1.1227504744859082E-4	1.0236842561489163E-4	0.0
15-19	1.3539049839388895E-4	1.4529712022758813E-4	7.264856011379406E-5	1.188794620043903E-4	1.3869270567178868E-4	0.0
20-24	1.4859932750548786E-4	1.8822581484028464E-4	8.915959650329271E-5	1.3539049839388895E-4	1.6511036389498651E-4	0.0
25-29	1.8822581484028464E-4	2.212478876192819E-4	1.1557725472649055E-4	1.849236075623849E-4	2.212478876192819E-4	0.0
30-34	3.335229350678728E-4	2.509677531203795E-4	1.188794620043903E-4	2.212478876192819E-4	3.665450078468701E-4	0.0
35-39	7.3309001569374E-4	2.641765822319784E-4	1.320882911159892E-4	2.6087437495407866E-4	4.391935679606641E-4	0.0
40-44	0.001017079841593117	2.641765822319784E-4	1.419949129496884E-4	2.674787895098782E-4	5.316553717418565E-4	0.0
45-49	0.0014001358858294853	2.839898258993768E-4	1.519015347833876E-4	2.9389644773307596E-4	6.769524919694448E-4	0.0
50-54	0.0019020713920702447	2.971986550109757E-4	1.6841257117288622E-4	3.5333617873527115E-4	7.991341612517348E-4	0.0
55-59	0.0023577759964204074	3.4012734962367215E-4	1.8162140028448517E-4	3.830560442363687E-4	8.5527168497603E-4	0.0
60-64	0.0027540408697683746	3.8635825151426845E-4	1.8162140028448517E-4	4.061714951816668E-4	9.246180378119244E-4	0.0
65-69	0.003021519659278253	4.391935679606641E-4	1.8162140028448517E-4	4.4579798251646353E-4	0.0010269864634268162	0.0
70-74	0.005323158131974365	4.7882005529546084E-4	1.8162140028448517E-4	4.722156407396615E-4	0.0010732173653174122	0.0
75-79	0.04715551992840814	4.953310916849595E-4	1.8162140028448517E-4	5.085399207965585E-4	0.0011227504744859083	0.0
80-84	0.06521859373851968	5.580730299650545E-4	1.8162140028448517E-4	5.679796517987536E-4	0.001235025521934499	0.0
85-89	0.06541012176063786	6.37326004634648E-4	1.849236075623849E-4	6.142105536893498E-4	0.0015751528715581714	0.0
90-94	0.06546956149164004	6.604414555799461E-4	2.278523021750814E-4	6.703480774136451E-4	0.0015949661152255697	0.0
95-99	0.06560825419731184	6.934635283589433E-4	2.311545094529811E-4	7.09974564748442E-4	0.001614779358892968	0.0
100-104	0.06578327118304052	7.264856011379407E-4	2.311545094529811E-4	7.396944302495396E-4	0.001637894809838266	0.0
105-109	0.06588233740137751	8.057385758075342E-4	2.4436333856458E-4	7.826231248622362E-4	0.0016808235044509627	0.0
110-114	0.06602433231432721	8.387606485865314E-4	2.4766554584247977E-4	7.958319539738349E-4	0.0017138455772299603	0.0
115-119	0.06619934930005589	8.68480514087629E-4	2.4766554584247977E-4	8.354584413086316E-4	0.0018195162101227514	0.0
120-124	0.06662203183162707	9.18013623256125E-4	2.7738541134357737E-4	8.618760995318296E-4	0.0020110442322409357	0.0
125-129	0.0673088909454302	9.246180378119244E-4	2.9059424045517625E-4	8.81689343199228E-4	0.002103506036022128	0.0
130-134	0.06816746483768413	9.477334887572226E-4	3.137096914004744E-4	9.411290742014231E-4	0.0021398303160790255	0.0
135-139	0.06920766013022255	9.576401105909217E-4	3.2361631323417356E-4	9.708489397025207E-4	0.0022884296435845128	1.651103638949865E-5
140-144	0.07009925609525548	9.774533542583203E-4	3.335229350678728E-4	0.0010038710124815181	0.0024304245565342016	1.651103638949865E-5
145-149	0.07077951079450281	0.0010236842561489163	3.467317641794717E-4	0.001040195292538415	0.002479957665702697	1.651103638949865E-5
150-154	0.07137390810452476	0.001040195292538415	3.467317641794717E-4	0.0010467997070942145	0.002506375323925895	1.651103638949865E-5
155-159	0.07204425618193841	0.0010600085362058133	3.467317641794717E-4	0.001063310743483713	0.0025658150549280902	1.651103638949865E-5
160-164	0.07293585214697133	0.0010996350235406103	3.599405932910705E-4	0.0010798217798732117	0.002608743749540787	1.651103638949865E-5
165-167	0.07367224436994298	0.0011062394380964096	3.632428005689704E-4	0.0011172467956894088	0.0026252547859302853	1.651103638949865E-5
>>END_MODULE
