##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479209
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6927656
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.20701331012972	39.0	39.0	39.0	37.0	39.0
2	39.943178471910265	41.0	40.0	41.0	38.0	41.0
3	39.992421246089584	41.0	40.0	41.0	38.0	41.0
4	40.01066507921294	41.0	40.0	41.0	38.0	41.0
5	40.016633764725036	41.0	40.0	41.0	38.0	41.0
6	40.001877402688585	41.0	40.0	41.0	38.0	41.0
7	40.026108542341014	41.0	40.0	41.0	38.0	41.0
8	39.99857282174519	41.0	40.0	41.0	38.0	41.0
9	39.98371570412849	41.0	40.0	41.0	38.0	41.0
10-14	39.91973230195033	41.0	40.0	41.0	38.0	41.0
15-19	39.79108494994555	41.0	40.0	41.0	37.8	41.0
20-24	39.61397552072447	40.4	39.0	41.0	37.0	41.0
25-29	39.38406684743007	40.0	38.8	41.0	36.2	41.0
30-34	39.29505220813505	40.0	38.8	41.0	35.4	41.0
35-39	39.13524912322436	40.0	38.2	41.0	35.0	41.0
40-44	38.64493655574122	40.0	36.8	41.0	35.0	41.0
45-49	38.02397653694121	39.0	35.4	41.0	34.6	41.0
50-54	37.33909942987931	37.4	35.0	40.0	34.0	41.0
55-59	36.63527986955472	36.2	35.0	39.0	34.0	41.0
60-64	35.892021024138614	35.2	34.8	37.6	33.2	39.8
65-69	35.486653609821275	35.0	35.0	36.6	33.0	39.0
70-74	35.230232650120044	35.0	35.0	36.0	33.0	37.8
75-79	35.04465117782985	35.0	35.0	35.2	33.0	37.0
80-84	34.97137528191354	35.0	35.0	35.0	33.0	37.0
85-89	34.80389615188746	35.0	34.4	35.0	32.6	37.2
90-94	39.063663770877255	40.2	38.6	40.2	36.4	40.2
95-99	39.59406745696048	41.0	39.0	41.0	36.8	41.0
100-104	39.48069053193518	41.0	39.0	41.0	36.0	41.0
105-109	39.334619815972175	40.0	39.0	41.0	35.6	41.0
110-114	39.14127207464402	40.0	38.0	41.0	35.0	41.0
115-119	38.89171978388155	40.0	38.0	41.0	35.0	41.0
120-124	38.57619116541652	40.0	37.4	41.0	34.2	41.0
125-129	38.23510448023794	39.6	36.4	40.8	33.8	41.0
130-134	37.87344780114034	39.0	35.6	40.6	34.0	41.0
135-139	37.58898424882885	38.6	35.0	40.8	33.6	41.0
140-144	37.175134190740685	37.2	35.0	40.0	34.0	41.0
145-149	36.503163564925885	36.0	35.0	39.0	33.4	41.0
150-154	35.839591503877024	35.0	35.0	37.2	33.0	39.4
155-159	35.283107024154695	35.0	35.0	36.2	33.0	38.2
160-164	34.87547579237743	35.0	35.0	35.4	33.0	36.8
165-169	34.614360661421	35.0	34.4	35.0	33.0	36.0
170-174	34.45965317479816	35.0	34.0	35.0	33.0	36.0
175-179	34.20250751946539	34.8	33.8	35.0	32.4	35.4
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	55.0
30	593.0
31	3941.0
32	19923.0
33	73713.0
34	209296.0
35	506571.0
36	1162293.0
37	2133057.0
38	2235208.0
39	576548.0
40	6454.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.75370717021746	27.984732498264925	27.593315834389003	22.668244497128608
2	22.225107597721365	28.061121972569076	28.25192243956686	21.461847990142697
3	20.92813211279544	27.66352717282729	28.937984218615938	22.470356495761337
4	21.505513553213383	27.190726560325746	29.195777042047123	22.107982844413755
5	21.939368813924943	27.67062914209366	28.213208046127004	22.176793997854396
6	22.333556978002374	27.605484452461265	27.750468556752818	22.310490012783546
7	22.059322807021594	28.028109363397952	27.809088672994154	22.103479156586296
8	22.030308664287027	28.09974975662764	27.852826410549252	22.017115168536083
9	22.530723234525503	27.89670849707318	27.367900484666098	22.20466778373522
10-14	22.211270305569446	27.99043428253366	27.65361328564813	22.144682126248764
15-19	22.256639186472306	27.906108501923306	27.679480620862236	22.157771690742152
20-24	22.157887169917213	27.857110687944093	27.782158929369473	22.202843212769224
25-29	22.13460656822452	27.896656531444403	27.712626031084685	22.256110869246395
30-34	22.25451725663053	27.889297621013515	27.647873970647503	22.208311151708457
35-39	22.187819372093536	27.85082573384129	27.744019044825553	22.21733584923963
40-44	22.1591949715748	27.91608012868999	27.74842746233358	22.176297437401626
45-49	22.335589411483483	27.807899237491007	27.620958661919705	22.235552689105813
50-54	22.287434595482225	27.85020214629595	27.66169682790254	22.200666430319288
55-59	22.19904972186841	27.855462222720067	27.722883468809652	22.222604586601875
60-64	22.26525970689076	27.885053761329953	27.636695586501407	22.212990945277884
65-69	22.20815525482212	27.847748213825856	27.69749537217206	22.24660115917996
70-74	22.120876094309533	27.911527362213135	27.737878439691578	22.229718103785753
75-79	22.187354568413905	27.85287837617803	27.69572565381422	22.264041401593843
80-84	22.1343871577919	27.869969294087348	27.70341656687341	22.29222698124734
85-89	22.09509248149735	27.903010773052237	27.696505138245897	22.305391607204513
90-94	21.47169124152917	27.618501537882928	28.571826663937582	22.337980556650322
95-99	21.9459294791785	27.927343550999527	27.886766975531785	22.23995999429019
100-104	21.978484239826866	28.01638981232942	27.764192018702055	22.240933929141658
105-109	22.02339247227931	27.956729110439515	27.7920870575183	22.227791359762872
110-114	21.96425301943399	27.882184995469757	27.871141679347733	22.28242030574852
115-119	21.929207335610787	27.90265550595094	27.84729294858661	22.320844209851664
120-124	21.98357270360652	27.943427264786617	27.770555462507325	22.30244456909954
125-129	21.948644826151934	27.89992497396924	27.838115394156343	22.31331480572248
130-134	21.975598966969166	27.947729090311817	27.803289816587768	22.273382126131246
135-139	22.17829316707129	27.87534572747547	27.694757866075552	22.251603239377687
140-144	22.09727303457699	27.938075318798177	27.786556091425695	22.178095555199135
145-149	22.00077584626389	27.961825242596216	27.874057306134826	22.163341605005073
150-154	22.025101544223187	28.036236917644263	27.85225033819268	22.086411199939867
155-159	21.923301064588436	28.05190824502844	27.93720944656962	22.087581243813503
160-164	21.817647929420765	28.151054966473417	28.021349029764313	22.009948074341505
165-169	21.820858291114494	28.182611853932272	28.033749250231192	21.962780604722038
170-174	21.722074766947024	28.28953800309139	28.083567983764972	21.904819246196617
175-179	21.610381623922365	28.385085352500912	28.15565519689488	21.848877826681843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	3.5
9	7.0
10	18.0
11	45.5
12	95.0
13	174.5
14	303.0
15	521.0
16	885.5
17	1432.0
18	2411.5
19	3934.0
20	6043.0
21	8965.5
22	13095.5
23	18354.0
24	24946.0
25	33025.0
26	42469.0
27	53540.0
28	65771.0
29	79773.5
30	94823.5
31	110795.0
32	128246.0
33	145791.5
34	163786.0
35	182246.5
36	201145.0
37	219522.5
38	236265.5
39	251522.0
40	265291.5
41	277050.0
42	285734.5
43	290757.5
44	291627.0
45	288791.5
46	282364.5
47	272428.0
48	259439.5
49	243339.5
50	225611.0
51	207464.0
52	188924.0
53	170858.0
54	154075.5
55	139009.5
56	125228.0
57	113141.5
58	102562.0
59	92959.0
60	84578.0
61	76750.5
62	68967.5
63	61491.5
64	54203.5
65	46511.0
66	39200.5
67	32513.0
68	26157.5
69	20387.0
70	15384.0
71	11418.5
72	8256.0
73	5772.0
74	3891.5
75	2518.5
76	1657.5
77	1009.5
78	582.5
79	344.0
80	188.5
81	102.0
82	50.5
83	27.0
84	14.5
85	4.5
86	2.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1382.0
92-93	2943.0
94-95	4711.0
96-97	5575.0
98-99	6592.0
100-101	8177.0
102-103	8385.0
104-105	9695.0
106-107	10662.0
108-109	11254.0
110-111	12138.0
112-113	13396.0
114-115	13740.0
116-117	14895.0
118-119	16131.0
120-121	17218.0
122-123	16890.0
124-125	17974.0
126-127	18667.0
128-129	21248.0
130-131	20257.0
132-133	22517.0
134-135	96000.0
136-137	95144.0
138-139	74556.0
140-141	85981.0
142-143	76412.0
144-145	85177.0
146-147	79527.0
148-149	81152.0
150-151	76975.0
152-153	78614.0
154-155	81371.0
156-157	76717.0
158-159	82048.0
160-161	89720.0
162-163	77464.0
164-165	79586.0
166-167	94655.0
168-169	89918.0
170-171	93802.0
172-173	90931.0
174-175	95219.0
176-177	97329.0
178-179	4774911.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.7001271543378
#Duplication Level	Percentage of total
1	93.69475763296626
2	5.565243916095199
3	0.5273037225937031
4	0.12971639591461678
5	0.04629452797522201
6	0.020723612921813742
7	0.0052181450465876075
8	0.005255561841948889
9	0.0010586307121983698
>10	0.00442785393245014
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.4434896882870627E-5	1.4434896882870627E-5	0.0	0.0
2	2.0208855636018877E-4	0.0	1.4434896882870627E-5	4.330469064861188E-5	0.0	0.0
3	2.0208855636018877E-4	0.0	1.4434896882870627E-5	5.773958753148251E-5	0.0	0.0
4	2.0208855636018877E-4	2.8869793765741254E-5	1.4434896882870627E-5	5.773958753148251E-5	0.0	0.0
5	2.0208855636018877E-4	2.8869793765741254E-5	4.330469064861188E-5	7.217448441435314E-5	1.4434896882870627E-5	0.0
6	2.0208855636018877E-4	2.8869793765741254E-5	4.330469064861188E-5	7.217448441435314E-5	1.4434896882870627E-5	0.0
7	2.0208855636018877E-4	2.8869793765741254E-5	4.330469064861188E-5	7.217448441435314E-5	1.4434896882870627E-5	0.0
8	2.0208855636018877E-4	4.330469064861188E-5	4.330469064861188E-5	1.0104427818009439E-4	1.4434896882870627E-5	0.0
9	2.0208855636018877E-4	4.330469064861188E-5	4.330469064861188E-5	1.2991407194583564E-4	1.4434896882870627E-5	0.0
10-14	2.136364738664853E-4	4.9078649401760136E-5	4.330469064861188E-5	1.9920157698361467E-4	2.3095835012593E-5	0.0
15-19	2.453932470088007E-4	8.660938129722376E-5	5.485260815490838E-5	2.5116720576194893E-4	6.062656690805663E-5	0.0
20-24	3.002458551637091E-4	1.1836615443953916E-4	8.083542254407552E-5	2.6560210264481955E-4	8.949636067379787E-5	0.0
25-29	3.406635664357468E-4	1.6167084508815103E-4	9.815729880352027E-5	3.320026283060244E-4	1.1259219568639089E-4	0.0
30-34	4.417078446158412E-4	2.136364738664853E-4	1.0681823693324264E-4	3.724203395780622E-4	1.5878386571157688E-4	0.0
35-39	5.745088959382509E-4	2.598281438916713E-4	1.356880306989839E-4	4.1572503022667406E-4	2.6560210264481955E-4	0.0
40-44	6.957620297543642E-4	2.9735887578713493E-4	1.587838657115769E-4	5.138823290301944E-4	3.724203395780622E-4	5.773958753148251E-6
45-49	8.170151635704776E-4	3.291156489294503E-4	1.6167084508815103E-4	5.773958753148251E-4	4.907864940176013E-4	1.4434896882870625E-5
50-54	9.844599674117768E-4	4.012901333438035E-4	1.7321876259444752E-4	6.293615040931594E-4	6.062656690805664E-4	2.020885563601888E-5
55-59	0.0011605657093827985	4.214989889798224E-4	1.7610574197102165E-4	7.477276585326984E-4	7.621625554155692E-4	2.886979376574125E-5
60-64	0.0012991407194583567	4.4459482399241535E-4	2.0786251511333703E-4	8.429979779596447E-4	8.458849573362189E-4	3.175677314231538E-5
65-69	0.001405958956391599	4.474818033689895E-4	2.280713707493559E-4	9.2383340050372E-4	9.180594417505719E-4	5.196562877833426E-5
70-74	0.002280713707493559	4.619167002518601E-4	2.482802263853748E-4	9.700250705289062E-4	9.90233926164925E-4	6.9287505037779E-5
75-79	0.016646323085326407	4.850125352644531E-4	2.598281438916713E-4	0.0010191037199306662	0.0011114870599810383	7.217448441435314E-5
80-84	0.022648353209224016	5.225432671599167E-4	2.598281438916713E-4	0.0010421995549432593	0.0012587230081863188	7.217448441435314E-5
85-89	0.022752284466780685	5.860568134445475E-4	2.742630407745419E-4	0.0010624084105792784	0.001498342296441971	7.217448441435314E-5
90-94	0.02283311988932476	6.149266072102887E-4	2.858109582808384E-4	0.0010970521630981676	0.0015012292758185453	7.217448441435314E-5
95-99	0.022887972497479668	6.380224422228817E-4	3.031328345402832E-4	0.0012211922762908548	0.0015012292758185453	7.217448441435314E-5
100-104	0.02298901677565976	6.553443184823265E-4	3.089067932934314E-4	0.0012904797813286341	0.0015156641727014158	7.217448441435314E-5
105-109	0.02308717407446328	6.640052566120488E-4	3.406635664357468E-4	0.0013539933276132646	0.0015416469870905831	7.217448441435314E-5
110-114	0.023130478765111897	6.69779215365197E-4	3.6087242207176565E-4	0.0014636985439230817	0.0015791777189860467	7.217448441435314E-5
115-119	0.023217088146409114	6.813271328714936E-4	3.753073189546363E-4	0.001561855842726602	0.0015993865746220654	7.217448441435314E-5
120-124	0.0233643240946144	7.188578647669573E-4	4.0995107147352575E-4	0.0016426912652706773	0.0016888829352958636	7.217448441435314E-5
125-129	0.02352310796032598	7.246318235201055E-4	4.7057763838158253E-4	0.0017119787703084564	0.001772605337216513	7.217448441435314E-5
130-134	0.023834901732995982	7.765974522984398E-4	4.965604527707496E-4	0.0017985881516056803	0.0019429371204343863	7.217448441435314E-5
135-139	0.024140921546912837	8.343370398299222E-4	5.196562877833426E-4	0.001859214718513737	0.002026659522355036	7.217448441435314E-5
140-144	0.024475811154595437	8.603198542190894E-4	5.485260815490838E-4	0.0018967454504092006	0.002069964213003648	7.217448441435314E-5
145-149	0.024781830968512292	9.007375654911271E-4	5.687349371851027E-4	0.0019371631616812383	0.002130590779911705	7.217448441435314E-5
150-154	0.02502433723614452	9.2383340050372E-4	5.860568134445475E-4	0.002000676707965869	0.0022027652643260573	7.217448441435314E-5
155-159	0.02527839142128304	9.440422561397391E-4	5.918307721976958E-4	0.002052642336744203	0.0022893746456232817	7.217448441435314E-5
160-164	0.02552667164766842	0.0010248776786838147	6.004917103274182E-4	0.002093060048016241	0.00239330590317995	7.217448441435314E-5
165-168	0.025903422456311342	0.0010537474724495559	6.134831175220016E-4	0.002103886220678394	0.0025188895060609244	7.939193285578845E-5
>>END_MODULE
