##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479210
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6943042
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.249253569256815	39.0	39.0	39.0	37.0	39.0
2	39.96355343954422	41.0	40.0	41.0	38.0	41.0
3	39.995481519483825	41.0	40.0	41.0	38.0	41.0
4	40.00927417693858	41.0	40.0	41.0	38.0	41.0
5	40.00575482619866	41.0	40.0	41.0	38.0	41.0
6	39.99238950304492	41.0	40.0	41.0	38.0	41.0
7	40.02035577488945	41.0	40.0	41.0	38.0	41.0
8	40.00151504196575	41.0	40.0	41.0	38.0	41.0
9	39.983068660682164	41.0	40.0	41.0	38.0	41.0
10-14	39.92189230599498	41.0	40.0	41.0	38.0	41.0
15-19	39.79327453297849	41.0	40.0	41.0	37.8	41.0
20-24	39.61240413063899	40.4	39.2	41.0	37.0	41.0
25-29	39.38691982563262	40.0	38.8	41.0	36.2	41.0
30-34	39.298026196586456	40.0	38.8	41.0	35.4	41.0
35-39	39.14057616243715	40.0	38.2	41.0	35.0	41.0
40-44	38.65684041663582	40.0	36.8	41.0	35.0	41.0
45-49	38.02827869973997	39.0	35.4	41.0	34.6	41.0
50-54	37.32996139156295	37.4	35.0	40.0	34.0	41.0
55-59	36.61296716914575	36.2	35.0	39.0	34.0	41.0
60-64	35.86259339926217	35.2	34.8	37.6	33.0	39.6
65-69	35.45001657774791	35.0	34.8	36.4	33.0	39.0
70-74	35.18309663113085	35.0	35.0	36.0	33.0	37.4
75-79	34.98293961062024	35.0	35.0	35.0	33.0	37.0
80-84	34.901861518337356	35.0	35.0	35.0	33.0	36.4
85-89	34.721614963585125	35.0	34.2	35.0	32.4	36.4
90-94	39.08306635244535	40.2	39.0	40.2	36.4	40.2
95-99	39.61588078859097	41.0	39.0	41.0	37.0	41.0
100-104	39.50550822342824	41.0	39.0	41.0	36.0	41.0
105-109	39.364943416139354	40.0	39.0	41.0	36.0	41.0
110-114	39.17593712074534	40.0	38.2	41.0	35.0	41.0
115-119	38.93225419558304	40.0	38.0	41.0	35.0	41.0
120-124	38.611824734736764	40.0	37.6	41.0	34.4	41.0
125-129	38.27570852463053	39.8	36.8	40.8	33.8	41.0
130-134	37.915621174172486	39.0	35.6	40.6	34.0	41.0
135-139	37.61571153224415	38.6	35.0	40.8	33.8	41.0
140-144	37.200237412561584	37.2	35.0	40.0	34.0	41.0
145-149	36.52119470909864	36.0	35.0	39.0	33.6	41.0
150-154	35.849741518870175	35.0	35.0	37.2	33.0	39.6
155-159	35.28515642108815	35.0	35.0	36.2	33.0	38.2
160-164	34.8801737514876	35.0	35.0	35.4	33.0	36.8
165-169	34.61751674560541	35.0	34.4	35.0	33.0	36.0
170-174	34.47009358423635	35.0	34.0	35.0	33.0	36.0
175-179	34.19776375940061	34.8	33.8	35.0	32.4	35.4
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	45.0
30	541.0
31	3890.0
32	19955.0
33	74554.0
34	211850.0
35	513512.0
36	1169822.0
37	2146396.0
38	2235731.0
39	561889.0
40	4856.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.77485027456265	28.00939415316802	27.526637459488217	22.689118112781113
2	22.249627756824747	28.06216641063096	28.20932092878021	21.478884903764083
3	20.919706376542155	27.628149159979152	28.937258337195715	22.514886126282978
4	21.5108017494349	27.20415633377992	29.18467150277933	22.100370414005848
5	21.972155720792124	27.68254894612477	28.175862971878896	22.169432361204212
6	22.338191242397784	27.613703042556853	27.714292956891228	22.333812758154135
7	22.070873256995995	28.010071089876742	27.799687802551098	22.11936785057616
8	22.043968623551464	28.10031971576724	27.83192151221324	22.023790148468063
9	22.558210075641195	27.901372337946395	27.35819544228596	22.182222144126452
10-14	22.22501318586291	27.9853528179723	27.629672987719218	22.159961008445578
15-19	22.265133928327092	27.902694524964705	27.66233014289702	22.169841403811184
20-24	22.1610268236891	27.83587078977774	27.781102289169503	22.22200009736366
25-29	22.15233034741832	27.87199904595133	27.701892052503784	22.273778554126565
30-34	22.256402885075445	27.89438116606525	27.62856396374961	22.220651985109697
35-39	22.19611807043656	27.84846181255997	27.726247371109093	22.22917274589438
40-44	22.17239647981389	27.8947959698357	27.733045544013706	22.199762006336705
45-49	22.375826042821	27.809182199963644	27.580095295405098	22.234896461810255
50-54	22.302774489913787	27.834522677523772	27.6489844076991	22.213718424863337
55-59	22.21599696501908	27.8579475682273	27.6894220141546	22.23663345259902
60-64	22.279441777825916	27.857800658558595	27.628555322004388	22.234202241611097
65-69	22.221916560493224	27.823933659050315	27.689485386952867	22.264664393503597
70-74	22.14613421609721	27.89489967077831	27.724349067742928	22.23461704538155
75-79	22.205024829174302	27.847352788590364	27.671807256819132	22.275815125416205
80-84	22.156005969717597	27.8638786860284	27.68719244388843	22.292922900365575
85-89	22.11190426328978	27.877996417132433	27.699688407473268	22.310410912104523
90-94	21.46964912750294	27.606164284592865	28.56497622987584	22.359210358028356
95-99	21.957423550772624	27.912896851949704	27.881181207826007	22.248498389451665
100-104	21.99694067224661	27.99789125135595	27.754773372694174	22.250394703703265
105-109	22.043191949252876	27.933932654974065	27.784066473283964	22.2388089224891
110-114	21.977059933682362	27.85955419196821	27.87152246643132	22.291863407918104
115-119	21.929920588688915	27.890443821956335	27.826057142161716	22.353578447193033
120-124	21.986145345586415	27.9264256243288	27.76940158052205	22.31802744956273
125-129	21.949206180527888	27.899782832212782	27.830768172100218	22.32024281515911
130-134	21.98443418699143	27.932631249924434	27.807691874735664	22.275242688348477
135-139	22.19121653491194	27.84485187481314	27.67288738812759	22.29104420214733
140-144	22.10030221538712	27.91473950640669	27.78264729230689	22.2023109858993
145-149	22.00369544886328	27.950987723681596	27.865565012178163	22.17975181527696
150-154	22.040547835658618	28.03207899275269	27.813498044877328	22.113875126711363
155-159	21.94003222092717	28.040846566110478	27.91872295856489	22.100398254397465
160-164	21.835076450451396	28.146926936235673	27.985191784311652	22.03280482900128
165-169	21.833810420363367	28.177607230386858	28.002806289009	21.98577606024077
170-174	21.740326071314815	28.256436764804803	28.084838397834478	21.9183987660459
175-179	21.644818282965396	28.375137941597554	28.117027787821446	21.863015987615604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5
8	4.5
9	9.5
10	22.0
11	43.0
12	85.0
13	166.5
14	307.0
15	515.0
16	859.5
17	1462.0
18	2415.0
19	3834.0
20	5967.5
21	9016.5
22	13096.0
23	18416.5
24	24906.0
25	32946.0
26	42624.5
27	53240.0
28	65606.0
29	79953.5
30	95128.5
31	111259.0
32	128129.0
33	145457.0
34	164250.0
35	183033.0
36	201231.0
37	219001.0
38	236043.0
39	251990.5
40	265232.0
41	276504.5
42	285603.5
43	290797.0
44	292092.0
45	289512.5
46	282858.5
47	272594.5
48	259426.5
49	243932.0
50	226328.5
51	208018.0
52	189777.5
53	171760.0
54	154843.5
55	139192.5
56	125488.0
57	113684.0
58	102938.5
59	93547.5
60	85038.0
61	77051.5
62	69688.0
63	62400.5
64	54992.0
65	47336.0
66	39656.5
67	32854.0
68	26618.5
69	20802.5
70	15749.0
71	11635.0
72	8439.0
73	5874.5
74	4032.0
75	2644.0
76	1634.5
77	1000.5
78	578.0
79	325.5
80	182.0
81	94.0
82	49.0
83	22.5
84	14.5
85	6.5
86	2.5
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1264.0
92-93	2625.0
94-95	3774.0
96-97	4793.0
98-99	5933.0
100-101	6933.0
102-103	7992.0
104-105	9012.0
106-107	9960.0
108-109	10815.0
110-111	11682.0
112-113	12106.0
114-115	12718.0
116-117	13691.0
118-119	14117.0
120-121	14971.0
122-123	15625.0
124-125	16137.0
126-127	16949.0
128-129	17466.0
130-131	18266.0
132-133	19692.0
134-135	87004.0
136-137	80304.0
138-139	70048.0
140-141	72878.0
142-143	71246.0
144-145	76410.0
146-147	73195.0
148-149	77402.0
150-151	79433.0
152-153	74008.0
154-155	74476.0
156-157	75258.0
158-159	77805.0
160-161	74670.0
162-163	75439.0
164-165	78081.0
166-167	80688.0
168-169	82993.0
170-171	83663.0
172-173	90532.0
174-175	93745.0
176-177	97167.0
178-179	4950076.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.77035244920246
#Duplication Level	Percentage of total
1	93.84715651870363
2	5.389963878749338
3	0.557705918738319
4	0.10928329550529271
5	0.04320550667752256
6	0.010361007435609838
7	0.01460947338346789
8	0.00735701217314472
9	0.0031755135308641933
>10	0.015215747589414255
>50	0.0
>100	0.001966127513424147
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4402908696217019E-5	0.0	1.4402908696217019E-5	0.0	0.0	0.0
2	3.7447562610164246E-4	0.0	1.4402908696217019E-5	0.0	0.0	0.0
3	3.7447562610164246E-4	0.0	1.4402908696217019E-5	2.8805817392434038E-5	1.4402908696217019E-5	0.0
4	3.888785347978595E-4	0.0	2.8805817392434038E-5	2.8805817392434038E-5	1.4402908696217019E-5	0.0
5	3.888785347978595E-4	1.4402908696217019E-5	2.8805817392434038E-5	2.8805817392434038E-5	1.4402908696217019E-5	0.0
6	3.888785347978595E-4	4.3208726088651054E-5	4.3208726088651054E-5	5.7611634784868077E-5	1.4402908696217019E-5	0.0
7	3.888785347978595E-4	4.3208726088651054E-5	4.3208726088651054E-5	8.641745217730211E-5	1.4402908696217019E-5	0.0
8	3.888785347978595E-4	4.3208726088651054E-5	4.3208726088651054E-5	8.641745217730211E-5	2.8805817392434038E-5	0.0
9	3.888785347978595E-4	4.3208726088651054E-5	4.3208726088651054E-5	1.0082036087351912E-4	4.3208726088651054E-5	0.0
10-14	3.888785347978595E-4	8.35368704380587E-5	5.473105304562467E-5	1.613125773976306E-4	8.929803391654551E-5	0.0
15-19	3.888785347978595E-4	1.0946210609124934E-4	8.929803391654551E-5	2.2180479392174208E-4	1.1810385130897956E-4	0.0
20-24	4.004008617548331E-4	1.1522326956973615E-4	1.0946210609124934E-4	2.966999191420706E-4	1.3826792348368337E-4	0.0
25-29	4.032814434940765E-4	1.1522326956973615E-4	1.2962617826595314E-4	3.917591165371029E-4	1.469096687014136E-4	0.0
30-34	4.3496784262575386E-4	1.469096687014136E-4	1.555514139191438E-4	5.012212226283522E-4	2.0164072174703825E-4	0.0
35-39	5.329076217600296E-4	1.8435723131157784E-4	1.613125773976306E-4	5.933998382841412E-4	2.938193374028272E-4	2.0164072174703827E-5
40-44	6.510114730690093E-4	2.2756595740022886E-4	1.7859606783309103E-4	6.798172904614432E-4	3.7735620784088586E-4	4.3208726088651054E-5
45-49	8.641745217730212E-4	2.6789410174963657E-4	1.8723781305082123E-4	7.403095069855548E-4	4.34967842625754E-4	4.608930782789446E-5
50-54	0.0010254870991706518	3.082222460990442E-4	2.1892421218249868E-4	8.094434687273963E-4	4.6953482349667484E-4	6.337279826335487E-5
55-59	0.0011349492052619011	3.168639913167744E-4	2.6213293827114973E-4	8.872191756869683E-4	5.242658765422995E-4	7.201454348108509E-5
60-64	0.0012213666574392031	3.168639913167744E-4	3.111028278382876E-4	9.390696469933497E-4	5.789969295879242E-4	7.201454348108509E-5
65-69	0.0012588142200493676	3.168639913167744E-4	3.399086452307216E-4	0.0010110841904744346	6.682949635044696E-4	7.48951252203285E-5
70-74	0.002125869323561632	3.197445730560178E-4	3.4566980870920843E-4	0.0010370094261276251	8.152046322058833E-4	8.641745217730211E-5
75-79	0.017905696091136996	3.370280634914782E-4	3.5431155392693866E-4	0.0010571734983023293	8.900997574262118E-4	8.641745217730211E-5
80-84	0.02411911090268502	3.6295329914466884E-4	3.7447562610164246E-4	0.00109174047917325	0.0010427705896061121	8.641745217730211E-5
85-89	0.02423145359051551	3.8311737131937264E-4	3.7735620784088586E-4	0.0011320686235226575	0.0013884403983153207	8.641745217730211E-5
90-94	0.024271781734864918	3.888785347978595E-4	4.148037704510501E-4	0.0012040831670037428	0.001405723888750781	8.641745217730211E-5
95-99	0.02433227395138903	4.148037704510501E-4	4.34967842625754E-4	0.0012674559652670976	0.0014633355235356491	8.641745217730211E-5
100-104	0.024375482677477682	4.5225133306121444E-4	4.5225133306121444E-4	0.0013221870183127223	0.0014863801774495963	8.641745217730211E-5
105-109	0.024438855475741036	4.7529598697516163E-4	4.810571504536484E-4	0.001420126797446998	0.0015036636678850564	8.641745217730211E-5
110-114	0.024496467110525905	4.954600591498654E-4	4.896988956713786E-4	0.0015094248313635434	0.001512305413102787	8.641745217730211E-5
115-119	0.02455695932705002	5.06982386106839E-4	4.954600591498654E-4	0.0015785587931053853	0.0015180665765812738	9.505919739503231E-5
120-124	0.024637615615748832	5.185047130638127E-4	5.098629678460824E-4	0.001607364610497819	0.0015382306487559778	1.0082036087351912E-4
125-129	0.0248478980827136	5.329076217600296E-4	5.213852948030561E-4	0.0016736179905004177	0.0015843199565838718	1.0082036087351912E-4
130-134	0.02503801647750366	5.329076217600296E-4	5.588328574132204E-4	0.0017283490435460423	0.0016217675191940364	1.0082036087351912E-4
135-139	0.0252857465070786	5.473105304562466E-4	6.020415835018714E-4	0.00176867718789545	0.0017139461348498254	1.0082036087351912E-4
140-144	0.02550179013752186	5.703551843701938E-4	6.250862374158186E-4	0.0018262888226803179	0.0017917218418093972	1.0082036087351912E-4
145-149	0.025712072604486623	5.905192565448978E-4	6.8557845393993E-4	0.001855094640072752	0.0018349305678980478	1.0082036087351912E-4
150-154	0.025893549254058956	6.337279826335488E-4	6.913396174184169E-4	0.0019184674383361067	0.001875258712247456	1.0082036087351912E-4
155-159	0.02609230939406675	6.452503095905225E-4	7.115036895931206E-4	0.0020653771070375205	0.0019587955826855146	1.0082036087351912E-4
160-164	0.026334278260163196	6.596532182867394E-4	7.547124156817717E-4	0.002160436304432553	0.0020452130348628163	1.0082036087351912E-4
165-168	0.02662377672495716	6.769367087221999E-4	7.77757069595719E-4	0.0022036450305212042	0.002146033395736336	1.0082036087351912E-4
>>END_MODULE
