##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479211
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6906026
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23091804172182	39.0	39.0	39.0	37.0	39.0
2	39.92747044392824	41.0	40.0	41.0	38.0	41.0
3	39.949894483455466	41.0	40.0	41.0	38.0	41.0
4	39.96808236748602	41.0	40.0	41.0	38.0	41.0
5	39.96942351505772	41.0	40.0	41.0	38.0	41.0
6	39.9501005353875	41.0	40.0	41.0	38.0	41.0
7	39.97692681724627	41.0	40.0	41.0	38.0	41.0
8	39.953290937508775	41.0	40.0	41.0	38.0	41.0
9	39.93337934146208	41.0	40.0	41.0	38.0	41.0
10-14	39.870912562449085	41.0	40.0	41.0	38.0	41.0
15-19	39.73139513230909	41.0	39.8	41.0	37.6	41.0
20-24	39.54566675538146	40.0	39.0	41.0	37.0	41.0
25-29	39.304564187855654	40.0	38.6	41.0	36.0	41.0
30-34	39.23396207891484	40.0	38.4	41.0	35.4	41.0
35-39	39.10524770685775	40.0	38.0	41.0	35.0	41.0
40-44	38.61648696370387	40.0	36.8	41.0	35.0	41.0
45-49	37.992136577533884	39.0	35.4	41.0	34.4	41.0
50-54	37.30751795605751	37.4	35.0	40.0	34.0	41.0
55-59	36.59048772767436	36.2	35.0	39.0	33.8	41.0
60-64	35.84445520477334	35.2	34.8	37.6	32.8	39.6
65-69	35.435813505480574	35.0	34.8	36.4	33.0	39.0
70-74	35.17060144285585	35.0	35.0	36.0	33.0	37.4
75-79	34.9811260484684	35.0	35.0	35.2	33.0	37.0
80-84	34.90714917667556	35.0	34.6	35.0	33.0	37.0
85-89	34.72999988705516	35.0	33.8	35.0	32.4	37.2
90-94	39.071586916005955	40.2	38.6	40.2	36.4	40.2
95-99	39.59669057560326	41.0	39.0	41.0	36.8	41.0
100-104	39.486391215490016	41.0	39.0	41.0	36.0	41.0
105-109	39.33978617375415	40.0	39.0	41.0	35.8	41.0
110-114	39.157889835397995	40.0	38.2	41.0	35.0	41.0
115-119	38.9106663296199	40.0	38.0	41.0	35.0	41.0
120-124	38.59581303227888	40.0	37.6	41.0	34.2	41.0
125-129	38.26927982895188	39.8	36.8	40.8	33.8	41.0
130-134	37.901450789158034	39.0	35.6	40.6	34.0	41.0
135-139	37.599345412129104	38.6	35.0	40.8	33.6	41.0
140-144	37.19279075611984	37.2	35.0	40.0	34.0	41.0
145-149	36.51498998010227	36.0	35.0	39.0	33.4	41.0
150-154	35.84900368911728	35.0	35.0	37.2	33.0	39.6
155-159	35.287516288474166	35.0	35.0	36.2	33.0	38.2
160-164	34.879030167818485	35.0	35.0	35.4	33.0	36.8
165-169	34.614223525355854	35.0	34.4	35.0	33.0	36.0
170-174	34.46290895878862	35.0	34.0	35.0	33.0	36.0
175-179	34.202410755413766	34.8	33.8	35.0	32.4	35.4
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	61.0
30	558.0
31	4081.0
32	20622.0
33	77582.0
34	219455.0
35	527399.0
36	1181774.0
37	2130108.0
38	2191739.0
39	547829.0
40	4814.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.720509016328638	28.053789545536027	27.546276252073188	22.679425186062144
2	22.21202179082442	28.080910787187886	28.265575600207704	21.441491821779994
3	20.895997205918427	27.662015752619524	28.980096512813592	22.46189052864846
4	21.43823379755593	27.219561582884282	29.291114745296355	22.051089874263432
5	21.935538615116712	27.669197886020125	28.243507916130056	22.15175558273311
6	22.301146853487086	27.61520156454667	27.777147088644032	22.30650449332221
7	22.015845292212916	28.03270650877943	27.838093861795482	22.11335433721217
8	21.990201021542635	28.11480871922579	27.891482018747105	22.00350824048447
9	22.49147338860294	27.96184086187918	27.357093645462673	22.18959210405521
10-14	22.190791056969665	28.00001332169905	27.668036581385593	22.141159039945695
15-19	22.225887941922025	27.92812827521935	27.697031548968976	22.14895223388965
20-24	22.129641562310944	27.87629817785221	27.802733438883664	22.19132682095318
25-29	22.098657027934735	27.91244342259934	27.740758578088183	22.24814097137775
30-34	22.2121984481379	27.925443663258726	27.66426885737181	22.198089031231564
35-39	22.15860467365747	27.873297899544543	27.767509128983875	22.20058829781411
40-44	22.123307963219368	27.94316731503762	27.76055867730588	22.172966044437135
45-49	22.31722266901399	27.84426817970277	27.626736418310614	22.211772732972623
50-54	22.262418357532972	27.873619357934647	27.66898647644825	22.194975808084127
55-59	22.188862306629023	27.901655742390773	27.689209394809694	22.22027255617051
60-64	22.235595406098962	27.904968791023954	27.643915618041405	22.215520184835675
65-69	22.17636018167322	27.85405673248262	27.711763610504796	22.257819475339364
70-74	22.099786476332408	27.935411769373587	27.75762500749346	22.207176746800545
75-79	22.160304638297045	27.878418065614003	27.70537209098257	22.25590520510638
80-84	22.12049303028978	27.895035437167483	27.717271843459613	22.267199689083128
85-89	22.074950195669697	27.907010486204364	27.720521179619073	22.297518138506863
90-94	21.43476920310317	27.640369475257355	28.588676981674784	22.336184339964696
95-99	21.936120587880477	27.952780834963896	27.90086531379811	22.210233263357505
100-104	21.97324488518626	28.038737911338234	27.773101568439472	22.21491563503603
105-109	22.018833117549104	27.968112476412365	27.80031303736384	22.21274136867469
110-114	21.939718527018723	27.899090912659524	27.896530565361594	22.26465999496016
115-119	21.912553641435522	27.92186634690917	27.863654578063883	22.301925433591425
120-124	21.964623438515098	27.949381193632934	27.80595245972034	22.28004290813163
125-129	21.918068375198597	27.914140091928452	27.86852500863624	22.299266524236707
130-134	21.97589088767577	27.957949419449935	27.80134612765756	22.264813565216734
135-139	22.16876823204263	27.896839132594543	27.711741677002088	22.22265095836074
140-144	22.07297147481238	27.94894994142605	27.81607622311998	22.16200236064159
145-149	21.96116805538312	28.01021907655911	27.890262253081094	22.138350614976677
150-154	21.998722145887193	28.06725018625162	27.86443037385306	22.06959729400813
155-159	21.889457628218768	28.104523199747472	27.94952951987811	22.056489652155655
160-164	21.79558265571377	28.196757213833674	28.043238871068272	21.964421259384288
165-169	21.802885114517537	28.231416301679207	28.03124904413362	21.934449539669636
170-174	21.70590925795305	28.320861627706268	28.1290504307383	21.844178683602383
175-179	21.584829315201702	28.416701457258693	28.18454177457661	21.813927452962993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	4.5
9	13.5
10	20.5
11	50.5
12	99.5
13	176.0
14	321.0
15	536.0
16	882.0
17	1513.5
18	2463.5
19	3899.0
20	6106.5
21	9116.5
22	13190.5
23	18530.0
24	25106.5
25	33029.5
26	42585.5
27	53891.5
28	66182.0
29	79818.5
30	95137.0
31	111371.5
32	128477.5
33	145874.0
34	163745.0
35	182499.0
36	201273.0
37	219113.5
38	235521.5
39	250953.5
40	264944.5
41	276383.5
42	284952.5
43	290058.5
44	290823.5
45	287524.5
46	281407.0
47	271697.0
48	258178.5
49	242439.5
50	224835.0
51	206968.5
52	188943.0
53	170185.0
54	152645.0
55	137489.5
56	124138.5
57	112128.5
58	101550.5
59	92049.0
60	83640.5
61	75934.5
62	68471.5
63	60828.5
64	53302.0
65	46116.0
66	39033.5
67	32213.5
68	25866.5
69	20199.0
70	15201.5
71	11159.0
72	8107.5
73	5721.5
74	3868.5
75	2501.0
76	1578.5
77	998.0
78	580.5
79	328.0
80	193.0
81	106.5
82	56.0
83	24.5
84	12.0
85	8.5
86	4.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1311.0
92-93	2910.0
94-95	4080.0
96-97	5082.0
98-99	6140.0
100-101	7160.0
102-103	8347.0
104-105	9672.0
106-107	10629.0
108-109	11264.0
110-111	11991.0
112-113	12831.0
114-115	13640.0
116-117	14126.0
118-119	14982.0
120-121	15986.0
122-123	16299.0
124-125	17034.0
126-127	17774.0
128-129	18650.0
130-131	19722.0
132-133	20959.0
134-135	84553.0
136-137	83228.0
138-139	72572.0
140-141	71608.0
142-143	71227.0
144-145	74611.0
146-147	74291.0
148-149	77699.0
150-151	78642.0
152-153	75623.0
154-155	75229.0
156-157	76464.0
158-159	75896.0
160-161	80321.0
162-163	78017.0
164-165	79008.0
166-167	82571.0
168-169	86118.0
170-171	84950.0
172-173	88540.0
174-175	93672.0
176-177	101480.0
178-179	4879117.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.81491812912961
#Duplication Level	Percentage of total
1	93.92882479999727
2	5.329800566527645
3	0.5303712186096337
4	0.11542509124238635
5	0.044241113423518726
6	0.021762309592136903
7	0.007306411265160621
8	0.0052564041822776445
9	0.0042352953649802474
>10	0.010879373074335836
>50	0.0
>100	0.0018974167206339322
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4480107662496492E-5	0.0	0.0	2.8960215324992985E-5	1.4480107662496492E-5	0.0
2	3.0408226091242633E-4	1.4480107662496492E-5	0.0	4.3440322987489474E-5	1.4480107662496492E-5	0.0
3	3.0408226091242633E-4	2.8960215324992985E-5	1.4480107662496492E-5	4.3440322987489474E-5	1.4480107662496492E-5	0.0
4	3.0408226091242633E-4	2.8960215324992985E-5	1.4480107662496492E-5	7.240053831248246E-5	1.4480107662496492E-5	0.0
5	3.0408226091242633E-4	4.3440322987489474E-5	2.8960215324992985E-5	7.240053831248246E-5	1.4480107662496492E-5	0.0
6	3.0408226091242633E-4	4.3440322987489474E-5	4.3440322987489474E-5	8.688064597497895E-5	2.8960215324992985E-5	0.0
7	3.0408226091242633E-4	4.3440322987489474E-5	4.3440322987489474E-5	8.688064597497895E-5	5.792043064998597E-5	0.0
8	3.0408226091242633E-4	4.3440322987489474E-5	4.3440322987489474E-5	1.0136075363747544E-4	5.792043064998597E-5	0.0
9	3.1856236857492285E-4	4.3440322987489474E-5	4.3440322987489474E-5	1.0136075363747544E-4	5.792043064998597E-5	0.0
10-14	3.3014645470492E-4	4.3440322987489474E-5	4.3440322987489474E-5	1.1873688283247125E-4	6.660849524748386E-5	0.0
15-19	3.59106670029913E-4	4.3440322987489474E-5	4.6336344519988775E-5	1.592811842874614E-4	7.240053831248246E-5	0.0
20-24	4.025469930174025E-4	4.923236605248808E-5	9.267268903997755E-5	2.2299365800244598E-4	7.819258137748106E-5	0.0
25-29	4.199231222123983E-4	8.398462444247966E-5	1.1873688283247125E-4	2.6353795945743613E-4	1.1584086129997192E-4	0.0
30-34	4.5177935906989055E-4	1.0715279670247403E-4	1.4190505509246561E-4	3.6779473462741094E-4	1.998254857424516E-4	0.0
35-39	5.647241988373632E-4	1.4480107662496493E-4	1.737612919499579E-4	4.3440322987489476E-4	2.9539419631492844E-4	2.027215072749509E-5
40-44	7.153173185273267E-4	1.4480107662496493E-4	1.8244935654745579E-4	4.546753806023899E-4	3.967549499524038E-4	4.923236605248808E-5
45-49	8.427422659572957E-4	1.4480107662496493E-4	1.882413996124544E-4	5.183878543173745E-4	5.589321557723646E-4	5.792043064998597E-5
50-54	9.875433425822608E-4	1.4480107662496493E-4	1.882413996124544E-4	5.676202203698625E-4	6.776690386048357E-4	5.792043064998597E-5
55-59	0.0010744239885572397	1.4769709815746422E-4	2.0561752880745017E-4	6.168525864223507E-4	7.500695769173183E-4	5.792043064998597E-5
60-64	0.0012684574312346929	1.708652704174586E-4	2.2878570106744462E-4	6.516048448123421E-4	8.485343090222945E-4	5.792043064998597E-5
65-69	0.001407466464794659	1.8534537807995507E-4	2.7801806711993265E-4	7.008372108648303E-4	9.441030195947713E-4	5.792043064998597E-5
70-74	0.002258896795349453	2.1140957187244878E-4	2.8960215324992986E-4	7.674457061123141E-4	0.0010657359239597418	8.977666750747825E-5
75-79	0.01721105596764333	2.3747376566494245E-4	3.0118623937992707E-4	8.253661367623001E-4	0.0011844728067922131	1.3032096896246843E-4
80-84	0.02340564602565933	2.7512204558743334E-4	3.214583901074221E-4	9.035587181397811E-4	0.001340857969547175	1.4480107662496493E-4
85-89	0.023512798822361805	3.127703255099242E-4	3.417305408349172E-4	9.383109765297727E-4	0.0016623163596545972	1.4480107662496493E-4
90-94	0.02357071925301179	3.214583901074221E-4	3.475225838999158E-4	9.643751703222663E-4	0.001688380553447091	1.4480107662496493E-4
95-99	0.02361705559753178	3.533146269649144E-4	3.620026915624123E-4	0.0010222956009722523	0.0016941725965120898	1.4480107662496493E-4
100-104	0.023669183985116766	3.851708638224067E-4	3.620026915624123E-4	0.001077320010089739	0.001708652704174586	1.4769709815746422E-4
105-109	0.02373579248036425	3.9096290688740525E-4	3.620026915624123E-4	0.0011323444192072255	0.0017318208764345803	1.5928118428746143E-4
110-114	0.023802400975611735	4.0833903608240103E-4	3.7358677769240946E-4	0.0011873688283247125	0.001737612919499579	1.5928118428746143E-4
115-119	0.023877697535456717	4.4019527293989345E-4	3.764827992249088E-4	0.0012771454958321906	0.0017578850702270742	1.5928118428746143E-4
120-124	0.023973266246029194	4.865316174598821E-4	3.764827992249088E-4	0.0013669221633396688	0.0018244935654745582	1.5928118428746143E-4
125-129	0.024216532054759134	4.923236605248807E-4	3.764827992249088E-4	0.001398778400197161	0.0019750866851645215	1.5928118428746143E-4
130-134	0.02455247055252905	4.923236605248807E-4	3.9385892841990456E-4	0.001474074960042143	0.0021025116325944903	1.5928118428746143E-4
135-139	0.024789944318193997	5.183878543173745E-4	4.4019527293989334E-4	0.001552267541419624	0.0021720161493744743	1.5928118428746143E-4
140-144	0.02508823453604142	5.705162419023617E-4	4.5177935906989055E-4	0.001647836251992101	0.002258896795349453	1.5928118428746143E-4
145-149	0.02538942077542135	6.197486079548499E-4	4.7205150979738565E-4	0.0016941725965120898	0.0023081291614019408	1.5928118428746143E-4
150-154	0.025684814971736282	6.371247371498457E-4	4.7784355286238417E-4	0.001792637328617066	0.0024384501303644094	1.6507322735246E-4
155-159	0.025957040995791214	6.573968878773406E-4	5.010117251223786E-4	0.001833181630072056	0.0025021626040793938	1.737612919499579E-4
160-164	0.026258227235171138	6.660849524748386E-4	5.068037681873772E-4	0.0018650378669295483	0.002557187013196881	1.737612919499579E-4
165-168	0.026516697156946697	6.805650601373352E-4	5.068037681873772E-4	0.001882413996124544	0.0026353795945743613	1.737612919499579E-4
>>END_MODULE
