##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479212
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6866086
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.17649910006953	39.0	39.0	39.0	37.0	39.0
2	39.87300799902594	41.0	40.0	41.0	38.0	41.0
3	39.91741918175799	41.0	40.0	41.0	38.0	41.0
4	39.93377740972076	41.0	40.0	41.0	38.0	41.0
5	39.94176682319446	41.0	40.0	41.0	38.0	41.0
6	39.92633153735622	41.0	40.0	41.0	38.0	41.0
7	39.948975151199676	41.0	40.0	41.0	38.0	41.0
8	39.93586870307188	41.0	40.0	41.0	38.0	41.0
9	39.92159099084981	41.0	40.0	41.0	38.0	41.0
10-14	39.8546816046289	41.0	40.0	41.0	38.0	41.0
15-19	39.72871190369593	41.0	39.8	41.0	37.4	41.0
20-24	39.56406721383915	40.0	39.0	41.0	37.0	41.0
25-29	39.34904310840266	40.0	38.8	41.0	36.0	41.0
30-34	39.24644649076636	40.0	38.4	41.0	35.4	41.0
35-39	38.862806641221795	40.0	37.8	41.0	35.0	41.0
40-44	38.58542800075618	40.0	36.8	41.0	35.0	41.0
45-49	37.99188687703592	39.0	35.4	41.0	34.4	41.0
50-54	37.30641294035642	37.4	35.0	40.0	34.0	41.0
55-59	36.60287642770568	36.2	35.0	39.0	33.8	41.0
60-64	35.850144667573346	35.2	34.8	37.6	32.8	39.8
65-69	35.439168894767704	35.0	34.8	36.4	33.0	39.0
70-74	35.17916504395663	35.0	35.0	36.0	33.0	37.6
75-79	34.993722041931896	35.0	35.0	35.2	33.0	37.0
80-84	34.925862361758945	35.0	35.0	35.0	33.0	37.0
85-89	34.76193473253903	35.0	34.0	35.0	32.6	37.2
90-94	39.02063796036601	40.2	38.6	40.2	36.4	40.2
95-99	39.547690326775005	41.0	39.0	41.0	36.0	41.0
100-104	39.4519163694733	41.0	39.0	41.0	36.0	41.0
105-109	39.309671053945195	40.0	39.0	41.0	35.6	41.0
110-114	39.13621339192086	40.0	38.0	41.0	35.0	41.0
115-119	38.892272425782004	40.0	38.0	41.0	35.0	41.0
120-124	38.586945599430514	40.0	37.4	41.0	34.2	41.0
125-129	38.23778320961632	39.6	36.4	40.8	33.8	40.8
130-134	37.85304545882929	39.0	35.6	40.6	34.0	41.0
135-139	37.52232714750774	38.4	35.0	40.4	33.2	41.0
140-144	37.17628358243532	37.2	35.0	40.0	34.0	41.0
145-149	36.515184508662045	36.0	35.0	39.0	33.4	41.0
150-154	35.85304861768894	35.0	35.0	37.2	33.0	39.6
155-159	35.29460231981146	35.0	35.0	36.2	33.0	38.2
160-164	34.88610045734756	35.0	35.0	35.6	33.0	36.8
165-169	34.62930930560454	35.0	34.8	35.0	33.0	36.0
170-174	34.49011439320635	35.0	34.0	35.0	33.0	36.0
175-179	34.24442715300496	35.0	33.8	35.0	32.4	35.6
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	116.0
30	888.0
31	5552.0
32	24873.0
33	86457.0
34	229806.0
35	531508.0
36	1161159.0
37	2090887.0
38	2168214.0
39	561004.0
40	5620.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.68481431779328	28.05275086854432	27.57675916089603	22.685675652766367
2	22.20129488619863	28.10771668167279	28.282576128525044	21.408412303603537
3	20.906787360368046	27.635147593548933	29.00792387395089	22.450141172132128
4	21.44179376722051	27.242289129498232	29.236394650460245	22.07952245282101
5	21.940738872190067	27.69285150229694	28.234921613274288	22.131488012238705
6	22.2906762309706	27.671689518599095	27.79124817253964	22.246386077890666
7	22.035086656357056	28.009523912167715	27.842441822022035	22.112947609453187
8	21.98469404548676	28.133466431967207	27.883003504471105	21.998836018074925
9	22.483639150456316	27.974365599265727	27.39706435369438	22.144930896583585
10-14	22.17074181709929	28.032535566842594	27.6605041067065	22.136218509351615
15-19	22.22010035994306	27.941173472047975	27.703597071169806	22.135129096839158
20-24	22.114197229688067	27.891165359711483	27.816983358495655	22.177654052104796
25-29	22.10537415348424	27.930005537361463	27.741044898068566	22.223575411085733
30-34	22.151152781948845	27.971120664669797	27.69965013546291	22.178076417918447
35-39	22.12336693714585	27.913562399305807	27.727511714825592	22.23555894872275
40-44	22.12362909523708	27.957526311205537	27.74380338376187	22.175041209795506
45-49	22.325109239820183	27.838253118297672	27.609118207957195	22.227519433924947
50-54	22.262875821829205	27.891086712284118	27.649053623854986	22.19698384203169
55-59	22.182294250319615	27.901238056150184	27.712251201048165	22.204216492482036
60-64	22.238937875231972	27.903906242945396	27.65554640591452	22.201609475908107
65-69	22.17515772450272	27.870740331536776	27.70970244182785	22.244399502132655
70-74	22.10223990786017	27.93332911938475	27.739967137026827	22.224463835728244
75-79	22.14727575506628	27.892362548328116	27.686690787152973	22.273670909452633
80-84	22.111625167526302	27.90629188157562	27.707197375622734	22.274885575275345
85-89	22.062141371372277	27.922630156394778	27.699891903480385	22.31533656875256
90-94	21.428464248739324	27.663779389058558	28.597704487704483	22.310051874497635
95-99	21.932148052496146	27.95891000754427	27.90258769276161	22.206354247197975
100-104	21.959036909723853	28.055497172530835	27.78001217779701	22.205453739948307
105-109	22.013983616156153	27.974956136983835	27.813683499623572	22.197376747236444
110-114	21.93790680979149	27.89899331117111	27.909160515085645	22.253939363951755
115-119	21.905022387848028	27.92568498848042	27.854092363940445	22.315200259731107
120-124	21.964195648702294	27.97099631796391	27.793653797966623	22.271154235367177
125-129	21.931153922272212	27.932445246323095	27.841237154009697	22.295163677394996
130-134	21.959313513250564	27.962533068631007	27.824940215690606	22.253213202427823
135-139	22.1471371159974	27.898784873105203	27.716278060139608	22.23779995075779
140-144	22.071538713558486	27.97145720230157	27.798641432112042	22.1583626520279
145-149	21.966349774424398	28.006658710751275	27.89790650686263	22.129085007961695
150-154	22.005396810024102	28.062565813143753	27.865637837690084	22.066399539142058
155-159	21.904770361093462	28.08038349181732	27.9576821209297	22.05716402615952
160-164	21.80369608908907	28.216570638936844	28.01752417708415	21.962209094889936
165-169	21.809000036711833	28.22352346539705	28.02542732062831	21.942049177262803
170-174	21.712172236394903	28.322611664799503	28.095661740803656	21.869554358001945
175-179	21.60370490247181	28.427800908500878	28.15015478238378	21.818339406643535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	1.0
8	4.0
9	6.5
10	19.0
11	46.0
12	95.0
13	179.0
14	313.0
15	568.5
16	967.0
17	1537.5
18	2504.5
19	4003.0
20	6115.5
21	9213.5
22	13396.0
23	18671.5
24	25180.0
25	33358.5
26	42856.0
27	53696.5
28	66100.0
29	80026.5
30	94994.0
31	110583.0
32	127412.0
33	145269.0
34	163614.5
35	181918.0
36	199829.0
37	217846.5
38	234916.5
39	249799.5
40	263196.0
41	274929.0
42	283062.0
43	287792.5
44	289063.5
45	286096.5
46	279072.5
47	268563.5
48	255636.5
49	240424.0
50	223200.0
51	204691.0
52	186579.0
53	169071.0
54	152080.0
55	136885.5
56	123490.0
57	111398.0
58	100950.5
59	91871.5
60	83398.5
61	75330.0
62	67703.0
63	60616.0
64	53645.5
65	46191.5
66	38860.0
67	32077.5
68	25876.0
69	20091.5
70	15145.0
71	11229.0
72	8043.5
73	5599.0
74	3778.0
75	2476.5
76	1550.5
77	978.0
78	611.0
79	349.5
80	193.0
81	95.0
82	41.0
83	21.0
84	12.5
85	3.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1396.0
92-93	2969.0
94-95	4310.0
96-97	5510.0
98-99	6810.0
100-101	7801.0
102-103	8842.0
104-105	10049.0
106-107	11166.0
108-109	11804.0
110-111	12620.0
112-113	13446.0
114-115	13996.0
116-117	14684.0
118-119	15467.0
120-121	17444.0
122-123	17458.0
124-125	18961.0
126-127	19415.0
128-129	19553.0
130-131	20462.0
132-133	23274.0
134-135	95085.0
136-137	89351.0
138-139	77633.0
140-141	78562.0
142-143	73251.0
144-145	80352.0
146-147	77621.0
148-149	80327.0
150-151	76549.0
152-153	73103.0
154-155	73388.0
156-157	73787.0
158-159	79381.0
160-161	81814.0
162-163	77988.0
164-165	82473.0
166-167	78975.0
168-169	81388.0
170-171	84344.0
172-173	88033.0
174-175	90362.0
176-177	104329.0
178-179	4790553.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.78181971175832
#Duplication Level	Percentage of total
1	93.8797648045051
2	5.337006565310717
3	0.5659045876991308
4	0.11543798561036626
5	0.04013177621583386
6	0.019693506613662426
7	0.012528197049684255
8	0.010515681111532008
9	0.00847324326094452
>10	0.008776111702838981
>50	0.0017675409201677263
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4564338401820193E-5	0.0	0.0	0.0	0.0	0.0
2	3.3497978324186444E-4	0.0	0.0	0.0	0.0	0.0
3	3.3497978324186444E-4	1.4564338401820193E-5	1.4564338401820193E-5	2.9128676803640387E-5	0.0	0.0
4	3.3497978324186444E-4	1.4564338401820193E-5	2.9128676803640387E-5	2.9128676803640387E-5	0.0	0.0
5	3.3497978324186444E-4	1.4564338401820193E-5	2.9128676803640387E-5	4.369301520546058E-5	1.4564338401820193E-5	0.0
6	3.3497978324186444E-4	1.4564338401820193E-5	2.9128676803640387E-5	4.369301520546058E-5	1.4564338401820193E-5	0.0
7	3.3497978324186444E-4	1.4564338401820193E-5	5.825735360728077E-5	5.825735360728077E-5	1.4564338401820193E-5	0.0
8	3.3497978324186444E-4	2.9128676803640387E-5	5.825735360728077E-5	7.282169200910097E-5	2.9128676803640387E-5	0.0
9	3.3497978324186444E-4	2.9128676803640387E-5	5.825735360728077E-5	8.738603041092116E-5	2.9128676803640387E-5	0.0
10-14	3.524569893240487E-4	5.24316182465527E-5	5.825735360728077E-5	1.0486323649310537E-4	2.9128676803640387E-5	0.0
15-19	3.75759930766961E-4	1.2234044257528962E-4	5.825735360728077E-5	1.8933639922366253E-4	2.9128676803640387E-5	0.0
20-24	3.874114014884171E-4	1.3690478097710983E-4	6.408308896800885E-5	2.3594228210948714E-4	6.69959566483729E-5	0.0
25-29	3.9323713684914525E-4	1.4564338401820194E-4	8.156029505019307E-5	3.2041544484004425E-4	1.0195036881274134E-4	0.0
30-34	4.2236581365278557E-4	1.4564338401820194E-4	1.136018395341975E-4	3.81585666127689E-4	1.572948547396581E-4	0.0
35-39	5.24316182465527E-4	1.747720608218423E-4	1.3690478097710983E-4	4.311044166938777E-4	2.708966942738556E-4	0.0
40-44	7.078268463284614E-4	1.9807500226475466E-4	1.4564338401820194E-4	4.981003733422506E-4	3.728470630865969E-4	0.0
45-49	8.243415535430229E-4	2.2137794370766695E-4	1.572948547396581E-4	5.680091976709875E-4	5.505319915888033E-4	0.0
50-54	9.408562607575845E-4	2.708966942738556E-4	1.7185919314147827E-4	6.78698169524821E-4	6.524823604015446E-4	2.039007376254827E-5
55-59	0.0011127154538990629	3.0002537107749603E-4	1.9807500226475466E-4	7.748228029768342E-4	7.54432729214286E-4	3.495441216436846E-5
60-64	0.0012612717055976287	3.145897094793162E-4	2.126393406665748E-4	8.447316273055711E-4	8.418187596252071E-4	6.990882432873693E-5
65-69	0.0013806992804925543	3.2332831252040827E-4	2.1846507602730288E-4	9.000761132324879E-4	9.0881471627358E-4	7.282169200910097E-5
70-74	0.0022574724522821297	3.699341954062329E-4	2.330294144291231E-4	9.525077314790405E-4	0.0010340680265292339	7.282169200910097E-5
75-79	0.016609171513435748	3.903242691687811E-4	2.6798382659349153E-4	0.001051545232611418	0.0011156283215794267	7.864742736982905E-5
80-84	0.022478599889369285	3.961500045295092E-4	2.767224296345837E-4	0.0011039768508579707	0.0012496202348761727	8.738603041092116E-5
85-89	0.022571811655140932	4.078014752509654E-4	2.883739003560398E-4	0.0011680599398259796	0.0016224672979627697	8.738603041092116E-5
90-94	0.022638807611789307	4.1362721061169347E-4	3.1458970947931616E-4	0.0012408816318350805	0.001648683107086046	8.738603041092116E-5
95-99	0.02269706496539659	4.3693015205460576E-4	3.5828272468477673E-4	0.0013049647208030891	0.001660334577807502	8.738603041092116E-5
100-104	0.022737845112921685	4.3693015205460576E-4	3.81585666127689E-4	0.001369047809771098	0.0016836375192504141	8.738603041092116E-5
105-109	0.022755322319003866	4.51494490456426E-4	3.990628722098733E-4	0.0014651724432231117	0.0017098533283736905	8.738603041092116E-5
110-114	0.022787363863487873	4.51494490456426E-4	4.078014752509654E-4	0.001503039723067844	0.0017418948728576949	8.738603041092116E-5
115-119	0.02282814401101297	4.893617703011585E-4	4.078014752509654E-4	0.0015962514888394931	0.0017710235496613356	8.738603041092116E-5
120-124	0.022918442909104258	5.097518440637068E-4	4.2236581365278557E-4	0.0016982018576522343	0.0018321937709489804	8.738603041092116E-5
125-129	0.023200991074099567	5.243161824655269E-4	4.2236581365278557E-4	0.0017593720789398793	0.001899189727597353	1.0486323649310537E-4
130-134	0.02347771350373415	5.447062562280753E-4	4.2236581365278557E-4	0.0018292809032686163	0.001945795610483178	1.1651470721456155E-4
135-139	0.0236117054170309	5.796606683924437E-4	4.2236581365278557E-4	0.0018787996538348049	0.0020069658317708227	1.1651470721456155E-4
140-144	0.023908817920428028	5.883992714335359E-4	4.2236581365278557E-4	0.0019370570074420859	0.0020885261268210158	1.1651470721456155E-4
145-149	0.024179714614701885	5.971378744746279E-4	4.2236581365278557E-4	0.0020040529640904586	0.002187563627953393	1.1651470721456155E-4
150-154	0.02445643704433647	6.02963609835356E-4	4.340172843742418E-4	0.002108916200583564	0.0023011654674875903	1.1651470721456155E-4
155-159	0.024756462415413967	6.291794189586322E-4	4.51494490456426E-4	0.0022108665693963054	0.0023710742918163275	1.1651470721456155E-4
160-164	0.025001143300564542	6.612209634426368E-4	4.6023309349751815E-4	0.002269123923003586	0.002435157380784336	1.1651470721456155E-4
165-168	0.025247280619555307	6.990882432873693E-4	4.8426425186052146E-4	0.0023193708904898657	0.002508707289713528	1.1651470721456155E-4
>>END_MODULE
