##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479213
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7612218
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.92795752827888	41.0	40.0	41.0	38.0	41.0
2	39.96419755713775	41.0	40.0	41.0	38.0	41.0
3	39.9799029665204	41.0	40.0	41.0	38.0	41.0
4	39.93219466389428	41.0	40.0	41.0	38.0	41.0
5	39.88397862488962	40.0	40.0	41.0	38.0	41.0
6	40.007710499095005	41.0	40.0	41.0	38.0	41.0
7	40.03571416372994	41.0	40.0	41.0	38.0	41.0
8	40.05046689414307	41.0	40.0	41.0	38.0	41.0
9	40.04511681089533	41.0	40.0	41.0	38.0	41.0
10-14	39.94696901744012	41.0	40.0	41.0	38.0	41.0
15-19	39.72370428697654	40.0	39.6	41.0	37.8	41.0
20-24	39.44884631522639	40.0	38.8	41.0	36.6	41.0
25-29	39.067114867178006	40.0	38.0	41.0	35.4	41.0
30-34	38.61893928944231	40.0	37.6	41.0	35.0	41.0
35-39	38.09238264064429	39.2	36.4	40.4	34.2	41.0
40-44	37.35953266183391	38.2	35.0	40.0	33.2	41.0
45-49	36.51351527242126	36.8	34.8	39.6	32.2	41.0
50-54	35.84995190626437	35.8	34.0	39.0	31.4	40.0
55-59	35.36283099091487	35.0	34.0	38.0	31.0	40.0
60-64	34.99994916067827	34.8	33.4	37.6	30.6	40.0
65-69	35.291024114128106	35.0	34.0	37.0	31.0	40.0
70-74	35.230885925757775	35.0	34.0	37.8	31.0	40.6
75-79	35.39972147408285	35.0	34.0	38.8	31.0	41.0
80-84	35.799081003723224	35.0	34.0	40.0	31.0	41.0
85-89	38.581190896004294	39.6	37.4	40.8	34.2	41.0
90-94	39.080619740130636	40.0	38.0	41.0	35.2	41.0
95-99	38.93416435038266	40.0	38.0	41.0	35.0	41.0
100-104	38.65762026008798	40.0	37.8	41.0	34.2	41.0
105-109	38.31211698025414	40.0	36.8	41.0	34.0	41.0
110-114	37.941949865758076	39.2	35.8	41.0	33.0	41.0
115-119	37.517313649300995	38.6	35.0	40.0	33.0	41.0
120-124	36.87390173074395	37.8	34.8	39.6	32.4	40.6
125-129	36.81748020225982	37.6	35.0	40.0	32.0	41.0
130-134	36.34470701212784	36.4	34.2	39.6	32.0	41.0
135-139	35.864606163193066	35.4	34.0	38.8	31.4	40.6
140-144	35.25631173680028	35.0	34.0	37.4	31.0	39.8
145-149	34.680042145417765	35.0	33.8	36.2	31.0	38.8
150-154	34.130347452328905	35.0	33.0	35.4	30.2	37.2
155-159	33.6851329457377	34.4	33.0	35.0	30.0	36.2
160-164	33.355607023081305	34.0	32.4	35.0	29.4	35.6
165-169	33.10931852334353	34.0	32.0	35.0	29.0	35.0
170-173	32.23509065334272	33.25	30.5	34.75	27.75	34.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	18.0
31	415.0
32	4860.0
33	37199.0
34	201859.0
35	833755.0
36	2118206.0
37	2802748.0
38	1451391.0
39	161116.0
40	650.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.360513059400034	26.824139823636163	28.11373767803287	22.70160943893094
2	22.3987016661898	26.979547879474815	27.9519582860081	22.669792168327284
3	22.256706258281096	27.31379999889651	27.780260628374016	22.64923311444838
4	22.36279885836165	27.50835564614676	27.59445407370099	22.534391421790602
5	22.6898782982831	27.391858719758154	27.36971011602663	22.548552865932113
6	22.248338132197475	27.616734045188934	27.53747987774391	22.597447944869682
7	22.354588373585727	27.503600133364547	27.589698560918773	22.55211293213095
8	22.779352877177192	27.34746955486561	27.328105947570076	22.54507162038712
9	22.413611906542876	27.32296946829426	27.368698584302237	22.894720040860626
10-14	22.52672742688136	27.35573784145436	27.44733795064724	22.670196781017044
15-19	22.375904105741583	27.26134222640497	27.627955478941878	22.73479818891156
20-24	22.503908847592122	27.178614695480345	27.471023557128817	22.84645289979872
25-29	22.464577341321544	27.267130289752608	27.445748400794617	22.822543968131235
30-34	22.405172841870794	27.207063696809524	27.509700851972447	22.878062609347236
35-39	22.415634969991665	27.24154510551327	27.51704430955603	22.825775614939037
40-44	22.490509336437817	27.168071119350497	27.408996957260022	22.932422586951663
45-49	22.693052143278084	27.13291710773391	27.31203178889517	22.86199896009284
50-54	22.677437771750625	27.07850195567179	27.34133730799617	22.902722964581415
55-59	22.530721006676373	27.158762400130946	27.40878676884976	22.90172982434292
60-64	22.36068383748337	27.135155614303212	27.57771519417862	22.92644535403479
65-69	22.363313819966795	27.156713063130876	27.587013403977657	22.892959712924668
70-74	22.22254801425813	27.141879016076526	27.749260465215265	22.886312504450082
75-79	22.08840314347277	27.15423283988977	27.85162222101364	22.905741795623825
80-84	21.98696884403468	27.16590617872478	27.957126293545453	22.889998683695083
85-89	22.026155845773204	27.245449355233912	28.22066314968909	22.507731649303793
90-94	22.380616933498874	27.667560744610718	27.305776628614133	22.646045693276275
95-99	22.4912218451865	27.63117302872322	27.193333694264137	22.68427143182614
100-104	22.477469754262295	27.62631546868069	27.238324119149	22.65789065790802
105-109	22.4866528434021	27.55381059589384	27.25392130567827	22.705615255025783
110-114	22.501592140622304	27.62384128154853	27.212034346832304	22.662532230996863
115-119	22.41352790287193	27.682358647113915	27.27460588685176	22.6295075631624
120-124	22.403199531521906	27.741036857001482	27.327974962128344	22.527788649348267
125-129	22.41211811101739	27.784566123563447	27.355088861143756	22.44822690427541
130-134	22.39412471120121	27.850821067436332	27.416362464532547	22.33869175682991
135-139	22.29309258708443	27.959069732248242	27.567114300780226	22.180723379887105
140-144	22.14917762001645	28.123826864261854	27.68934396894745	22.03765154677425
145-149	21.9539193077492	28.31293721880297	27.872317836506856	21.86082563694098
150-154	21.7672574133418	28.484355394473855	28.056442301238715	21.69194489094564
155-159	21.547326965906322	28.71393566062077	28.308358387257442	21.430378986215466
160-164	21.185254276311632	28.975274921737874	28.633786986451003	21.205683815499487
165-169	20.850347176269786	29.276873008101585	29.074787444624945	20.797992371003684
170-173	20.622597098546755	29.291041801569463	29.72216200643058	20.364199093453202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	2.5
9	5.0
10	15.0
11	41.0
12	85.0
13	165.5
14	349.5
15	619.0
16	1095.0
17	1911.0
18	3111.0
19	4940.5
20	7624.5
21	11163.5
22	15813.0
23	21797.5
24	29027.5
25	37360.5
26	46572.5
27	57254.5
28	69627.5
29	82815.5
30	96977.0
31	112059.5
32	128149.0
33	145636.0
34	163589.5
35	181946.0
36	201268.5
37	220711.5
38	238865.5
39	256300.0
40	273085.0
41	287407.5
42	298671.5
43	306433.0
44	309763.0
45	308338.5
46	302175.5
47	292057.5
48	279230.5
49	263453.5
50	245406.0
51	226502.5
52	208373.5
53	190979.5
54	174595.5
55	160319.0
56	148014.0
57	138174.0
58	129312.5
59	121104.0
60	113906.5
61	106191.0
62	98328.5
63	89836.5
64	79947.0
65	69704.0
66	59351.0
67	49311.5
68	39746.5
69	31037.0
70	23633.5
71	17435.5
72	12377.0
73	8539.5
74	5808.5
75	3840.0
76	2394.0
77	1432.0
78	833.5
79	502.0
80	286.0
81	138.5
82	82.0
83	41.0
84	14.5
85	7.0
86	5.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3574.0
92-93	8347.0
94-95	13638.0
96-97	18571.0
98-99	24327.0
100-101	31158.0
102-103	37689.0
104-105	43602.0
106-107	47649.0
108-109	52974.0
110-111	57967.0
112-113	64375.0
114-115	70928.0
116-117	77006.0
118-119	83893.0
120-121	90564.0
122-123	98474.0
124-125	105664.0
126-127	113076.0
128-129	123499.0
130-131	154769.0
132-133	166875.0
134-135	175609.0
136-137	180299.0
138-139	185767.0
140-141	192316.0
142-143	197311.0
144-145	202749.0
146-147	200195.0
148-149	192636.0
150-151	199260.0
152-153	208138.0
154-155	216239.0
156-157	228527.0
158-159	237502.0
160-161	243397.0
162-163	254573.0
164-165	261271.0
166-167	273180.0
168-169	284137.0
170-171	402653.0
172-173	1787840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.2087868374833
#Duplication Level	Percentage of total
1	94.7057586847416
2	4.5902925111832396
3	0.4770558243467831
4	0.13050138428116229
5	0.044129001073165794
6	0.025824967364441544
7	0.01351701813906122
8	0.0031397148803519593
9	0.0021068033780571867
>10	0.007674090612122471
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3136775641475323E-5	0.0	0.0	1.3136775641475323E-5	1.3136775641475323E-5	0.0
2	2.6273551282950646E-5	0.0	1.3136775641475323E-5	1.3136775641475323E-5	1.3136775641475323E-5	0.0
3	5.254710256590129E-5	0.0	1.3136775641475323E-5	1.3136775641475323E-5	1.3136775641475323E-5	0.0
4	5.254710256590129E-5	1.3136775641475323E-5	2.6273551282950646E-5	3.941032692442597E-5	1.3136775641475323E-5	0.0
5	5.254710256590129E-5	2.6273551282950646E-5	2.6273551282950646E-5	5.254710256590129E-5	2.6273551282950646E-5	0.0
6	6.568387820737661E-5	2.6273551282950646E-5	2.6273551282950646E-5	5.254710256590129E-5	2.6273551282950646E-5	0.0
7	6.568387820737661E-5	6.568387820737661E-5	2.6273551282950646E-5	6.568387820737661E-5	2.6273551282950646E-5	0.0
8	9.195742949032726E-5	7.882065384885194E-5	2.6273551282950646E-5	7.882065384885194E-5	3.941032692442597E-5	0.0
9	9.195742949032726E-5	1.0509420513180258E-4	3.941032692442597E-5	9.195742949032726E-5	3.941032692442597E-5	0.0
10-14	1.182309807732779E-4	1.1560362564498284E-4	4.46650371810161E-5	1.261130461581631E-4	4.991974743760623E-5	0.0
15-19	1.339951115430483E-4	1.261130461581631E-4	5.780181282249142E-5	1.7077808333917918E-4	7.619329872055687E-5	0.0
20-24	1.62896017954294E-4	1.339951115430483E-4	6.831123333567168E-5	2.6273551282950645E-4	8.1448008977147E-5	0.0
25-29	1.9179692436553972E-4	1.8128750385235944E-4	9.721213974691738E-5	3.4418352180665346E-4	1.1560362564498284E-4	0.0
30-34	2.574808025729163E-4	2.1807047564849038E-4	1.2348569102986804E-4	4.3876830642527575E-4	1.4713188718452362E-4	0.0
35-39	4.177494653989153E-4	2.758722884709818E-4	1.62896017954294E-4	5.018248295043573E-4	1.7603279359576935E-4	3.6782971796130906E-5
40-44	5.044521846326524E-4	3.074005500105226E-4	2.2595254103337557E-4	5.675087077117339E-4	2.390893166748509E-4	6.0429167950786484E-5
45-49	6.568387820737661E-4	3.23164680780293E-4	2.495987371880311E-4	5.964096141229797E-4	3.25792035908588E-4	6.568387820737661E-5
50-54	7.882065384885194E-4	3.415561666783584E-4	2.811269987275719E-4	6.410746513039958E-4	3.8359384873107945E-4	7.619329872055687E-5
55-59	9.37965780801338E-4	3.546929423198337E-4	3.1528261539540776E-4	6.831123333567167E-4	4.4665037181016093E-4	7.882065384885194E-5
60-64	0.0010483146961897307	3.704570730896041E-4	3.441835218066534E-4	7.645603423338638E-4	4.991974743760624E-4	8.670271923373713E-5
65-69	0.0010824703128575665	4.07240044885735E-4	3.65202362833014E-4	8.722819025939615E-4	6.04291679507865E-4	9.195742949032726E-5
70-74	0.021176482334058222	4.3088624104039056E-4	3.8096649360278435E-4	9.405931359296332E-4	7.146405948962575E-4	9.721213974691738E-5
75-79	0.03754753213846477	4.4927772693845603E-4	4.3088624104039056E-4	9.957675936238294E-4	8.486357064393059E-4	1.0509420513180258E-4
80-84	0.03769991873590588	4.702965679648166E-4	4.5978714745163637E-4	0.0010351779205482552	0.001087725023114157	1.0509420513180258E-4
85-89	0.037831286492320634	5.018248295043573E-4	4.781786333497017E-4	0.0010719608923443864	0.0011245079949102877	1.0509420513180258E-4
90-94	0.037912734501297785	5.202163154024227E-4	5.097068948892424E-4	0.0011245079949102877	0.001132390060295173	1.0509420513180258E-4
95-99	0.0379600268936071	5.543719320702587E-4	5.67508707711734E-4	0.0011534089013215333	0.0011428994808083532	1.0509420513180258E-4
100-104	0.037991555155146635	5.990369692512748E-4	5.780181282249142E-4	0.0011954465833742545	0.0011428994808083532	1.0509420513180258E-4
105-109	0.038041474902584235	6.542114269454711E-4	6.253105205342254E-4	0.0012427389756835654	0.0011455268359366483	1.0509420513180258E-4
110-114	0.038083512584636964	7.041311743830774E-4	6.699755577152415E-4	0.0013136775641475321	0.001184937162861074	1.1823098077327792E-4
115-119	0.038125550266689685	7.33032080794323E-4	6.778576231001266E-4	0.0013819887974832038	0.0012374842654269754	1.261130461581631E-4
120-124	0.038451342302598265	7.69815052590454E-4	7.120132397679625E-4	0.0014187717692793348	0.001305795498762647	1.4450453205622855E-4
125-129	0.039142336701339875	7.960886038734045E-4	7.35659435922618E-4	0.0014213991244076299	0.0013530878910719582	1.4450453205622855E-4
130-134	0.040014618603933835	8.092253795148798E-4	7.750697628470441E-4	0.0014476726756905806	0.001387243507739794	1.4450453205622855E-4
135-139	0.04063204705908317	8.17107444899765E-4	8.197348000280602E-4	0.0014975924231281868	0.0014476726756905806	1.4450453205622855E-4
140-144	0.04099462206678789	8.407536410544207E-4	8.433809961827157E-4	0.0015317480397960227	0.0015606489462072683	1.4713188718452362E-4
145-149	0.0412573575796174	8.56517771824191E-4	8.643998372090761E-4	0.001555394235950678	0.0016447243103127104	1.5764130769770388E-4
150-154	0.04187215867963844	8.933007436203219E-4	8.670271923373713E-4	0.0015737857218487438	0.0016998987680069068	1.5764130769770388E-4
155-159	0.04270240290017969	9.195742949032727E-4	8.775366128505515E-4	0.0016053139833882846	0.0017340543846747426	1.5764130769770388E-4
160-162	0.04337763316815152	9.195742949032726E-4	8.801639679788466E-4	0.0016158234039014647	0.001747191160316218	1.5764130769770388E-4
>>END_MODULE
