##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479214
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9321121
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.93822888899307	41.0	40.0	41.0	38.0	41.0
2	40.00497633278229	41.0	40.0	41.0	38.0	41.0
3	40.05161450001561	41.0	40.0	41.0	38.0	41.0
4	40.04520121560486	41.0	40.0	41.0	38.0	41.0
5	40.026267226871106	41.0	40.0	41.0	38.0	41.0
6	39.99198680072922	41.0	40.0	41.0	38.0	41.0
7	39.96479500695249	41.0	40.0	41.0	38.0	41.0
8	39.942381071976214	41.0	40.0	41.0	38.0	41.0
9	39.90161022477876	41.0	40.0	41.0	38.0	41.0
10-14	39.76422460345703	40.2	40.0	41.0	38.0	41.0
15-19	39.530930839756294	40.0	39.0	41.0	37.0	41.0
20-24	39.43952402291527	40.0	39.0	41.0	36.6	41.0
25-29	39.371776570650674	40.0	39.0	41.0	36.0	41.0
30-34	39.13448639922173	40.0	38.2	41.0	35.2	41.0
35-39	38.635537099024894	40.0	37.2	41.0	34.8	41.0
40-44	37.96877901273892	39.2	35.6	40.8	34.0	41.0
45-49	37.23531703965649	37.6	35.0	40.0	33.0	41.0
50-54	36.57988984372158	36.4	35.0	39.4	33.0	41.0
55-59	35.978009597772626	35.4	34.6	38.6	33.0	40.4
60-64	35.30046038453959	35.0	33.8	37.0	32.4	39.8
65-69	35.307140246328736	35.0	34.0	36.4	33.0	39.2
70-74	35.12623563195886	35.0	34.0	36.0	32.0	39.8
75-79	35.089813296061706	35.0	34.0	36.0	32.0	40.0
80-84	35.15665497744316	35.0	34.0	36.0	32.0	40.0
85-89	38.55602421640059	39.6	37.8	40.2	35.2	41.0
90-94	39.50561669966363	40.6	39.0	41.0	36.4	41.0
95-99	39.28990629761633	40.0	38.8	41.0	35.6	41.0
100-104	39.02594379000066	40.0	38.0	41.0	35.0	41.0
105-109	38.72128301631674	40.0	38.0	41.0	34.8	41.0
110-114	38.47004384755522	40.0	37.0	41.0	34.0	41.0
115-119	38.488527917407914	40.0	37.0	41.0	34.0	41.0
120-124	37.86157414257959	39.4	35.6	40.6	33.6	40.8
125-129	37.776781973496476	39.0	35.0	40.8	33.6	41.0
130-134	37.30462217370854	38.0	35.0	40.4	33.0	41.0
135-139	36.71146241062916	36.6	35.0	39.6	33.0	41.0
140-144	36.07143682110747	35.6	35.0	38.8	33.0	40.6
145-149	35.440384406978126	35.0	34.0	37.0	32.0	39.4
150-154	34.88711580610127	35.0	34.0	36.0	32.0	38.0
155-159	34.45046286885231	35.0	34.0	35.2	31.6	36.8
160-164	34.14781049991554	35.0	34.0	35.0	31.0	36.0
165-169	33.95086717409173	35.0	34.0	35.0	31.0	35.6
170-173	33.61392203647824	34.75	33.25	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	71.0
31	1234.0
32	10606.0
33	60389.0
34	251705.0
35	833775.0
36	1986643.0
37	3048172.0
38	2537392.0
39	585114.0
40	6019.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.205172532359573	27.00882222213401	28.238212978889553	22.54779226661686
2	22.20013022038873	27.161507719940552	28.101094278252585	22.537267781418137
3	22.073664744830584	27.56624444634932	27.855308390482218	22.50478241833788
4	22.135942661832196	27.704157042913614	27.69512379465946	22.464776500594724
5	22.468316847297658	27.6929995866377	27.459411802507443	22.379271763557195
6	22.02591297763434	27.83153442595585	27.636868998911186	22.505683597498628
7	22.15804300791718	27.789050265520636	27.64929239734148	22.4036143292207
8	22.58260567586238	27.608782248401237	27.40605985052656	22.40255222520982
9	22.158504325820896	27.628071773770557	27.45788838059285	22.755535519815695
10-14	22.332479108467748	27.5963481216476	27.50828146099595	22.562891308888705
15-19	22.185556865960653	27.522616646645826	27.666517793299754	22.625308694093768
20-24	22.334195640202502	27.41317058323779	27.506893215955465	22.745740560604244
25-29	22.390103078803506	27.50186163230796	27.420558106691246	22.68747718219729
30-34	22.28094668012571	27.4314816855183	27.536074255446312	22.751497378909683
35-39	22.3092136664678	27.476300329112775	27.51258566432085	22.701900340098575
40-44	22.44739876244499	27.354212009478257	27.365847948975237	22.832541279101516
45-49	22.63214907305677	27.30899212659078	27.257264442763912	22.801594357588534
50-54	22.626857863984384	27.269874514020366	27.26340962637434	22.839857995620914
55-59	22.547290181084442	27.37268618227357	27.240721368170203	22.839302268471787
60-64	22.384082343743845	27.39226322670846	27.35195906157639	22.8716953679713
65-69	22.405667730308405	27.414475147356203	27.297997740829672	22.881859381505723
70-74	22.313983479025755	27.436169962818852	27.337986493255478	22.91186006489992
75-79	22.201567815716587	27.46909733282081	27.356263264901294	22.973071586561318
80-84	22.157646059953517	27.476173734897337	27.398689492390453	22.96749071275869
85-89	21.755790961194474	27.460529693799707	28.32754772736026	22.45613161764556
90-94	22.140760990505388	27.838288830783853	27.515740643441138	22.505209535269618
95-99	22.304505414654386	27.828089895469603	27.31719095023621	22.5502137396398
100-104	22.224676870477968	27.81355675379058	27.39168889168405	22.570077484047403
105-109	22.27589140904178	27.67739247711973	27.40732014122252	22.639395972615965
110-114	22.362396195173638	27.74262187132923	27.24856087510954	22.6464210583876
115-119	22.32401592850263	27.780207588052345	27.298728018539254	22.597048464905768
120-124	22.322047531617617	27.80589147518408	27.345682683908045	22.526378309290255
125-129	22.354404134458562	27.83519496782495	27.334280402453597	22.476120495262894
130-134	22.3992013870547	27.851095637122764	27.305775618483572	22.443927357338964
135-139	22.33214911253794	27.98792800293104	27.4006128048656	22.27931007966542
140-144	22.17987041364139	28.138631263718146	27.505988901015733	22.17550942162473
145-149	21.975689569691095	28.313232482352653	27.665342320443443	22.045735627512812
150-154	21.86254153313572	28.467486246017653	27.796412173777707	21.87356004706892
155-159	21.629860249720487	28.723897159067803	27.971973182823675	21.67426940838804
160-164	21.389050391366172	28.899843972182964	28.15593100199249	21.55517463445838
165-169	21.20247736260809	29.15617108264021	28.351297068598846	21.290054486152858
170-173	20.924523355802457	29.39676035999187	28.55041849421277	21.1282977899929
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	2.0
8	2.0
9	7.0
10	21.5
11	51.5
12	112.0
13	213.0
14	424.0
15	790.0
16	1404.5
17	2424.0
18	4022.0
19	6354.5
20	9615.0
21	14032.5
22	19806.5
23	27016.0
24	35918.0
25	46696.5
26	58716.5
27	72473.0
28	87609.0
29	103109.0
30	120250.5
31	139075.0
32	159016.5
33	179628.5
34	201317.5
35	223306.5
36	245931.0
37	269600.0
38	292849.5
39	314974.5
40	335095.0
41	352977.5
42	367483.0
43	377047.0
44	381345.5
45	380206.0
46	373800.5
47	361828.5
48	345269.5
49	325807.0
50	303872.0
51	280886.5
52	257781.5
53	235351.5
54	214246.0
55	195926.0
56	180405.0
57	167382.5
58	156519.0
59	146277.0
60	136419.5
61	126975.0
62	117247.0
63	106092.0
64	93918.5
65	81352.5
66	68711.0
67	56490.0
68	44941.0
69	34481.0
70	25753.5
71	18657.0
72	12817.5
73	8573.0
74	5602.0
75	3555.0
76	2200.5
77	1256.5
78	686.0
79	397.0
80	207.5
81	93.0
82	40.0
83	23.0
84	12.5
85	2.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2070.0
92-93	5036.0
94-95	8118.0
96-97	11457.0
98-99	14881.0
100-101	18182.0
102-103	21874.0
104-105	25158.0
106-107	28681.0
108-109	31621.0
110-111	35155.0
112-113	38424.0
114-115	42054.0
116-117	45186.0
118-119	49228.0
120-121	52636.0
122-123	56013.0
124-125	59619.0
126-127	63258.0
128-129	68321.0
130-131	125567.0
132-133	143620.0
134-135	161244.0
136-137	165258.0
138-139	169558.0
140-141	172241.0
142-143	179832.0
144-145	184393.0
146-147	190102.0
148-149	181074.0
150-151	185026.0
152-153	190028.0
154-155	195047.0
156-157	201556.0
158-159	203610.0
160-161	210767.0
162-163	214670.0
164-165	220314.0
166-167	223741.0
168-169	232771.0
170-171	242804.0
172-173	4650926.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.17227161124623
#Duplication Level	Percentage of total
1	92.8028566608079
2	6.0929347597385926
3	0.745668655733811
4	0.18996667438533052
5	0.07864453465576991
6	0.03183297627537114
7	0.012388828738300054
8	0.007265679170448935
9	0.007304678612053678
>10	0.031136551882411177
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.145664668444922E-5	0.0	0.0	1.072832334222461E-5	0.0
2	0.0	4.291329336889844E-5	2.145664668444922E-5	1.072832334222461E-5	1.072832334222461E-5	0.0
3	0.0	4.291329336889844E-5	2.145664668444922E-5	1.072832334222461E-5	2.145664668444922E-5	0.0
4	1.072832334222461E-5	4.291329336889844E-5	2.145664668444922E-5	1.072832334222461E-5	2.145664668444922E-5	0.0
5	1.072832334222461E-5	4.291329336889844E-5	2.145664668444922E-5	5.364161671112305E-5	2.145664668444922E-5	0.0
6	2.145664668444922E-5	5.364161671112305E-5	3.218497002667383E-5	6.436994005334766E-5	2.145664668444922E-5	0.0
7	2.145664668444922E-5	5.364161671112305E-5	6.436994005334766E-5	7.509826339557227E-5	2.145664668444922E-5	0.0
8	3.218497002667383E-5	6.436994005334766E-5	7.509826339557227E-5	7.509826339557227E-5	2.145664668444922E-5	0.0
9	5.364161671112305E-5	6.436994005334766E-5	7.509826339557227E-5	7.509826339557227E-5	3.218497002667383E-5	0.0
10-14	6.0078610716457816E-5	8.153525740090704E-5	8.79722514062418E-5	1.351768741120301E-4	5.364161671112305E-5	0.0
15-19	8.582658673779689E-5	1.0942889809069103E-4	1.2015722143291563E-4	1.9740114949693283E-4	6.651560472179259E-5	0.0
20-24	1.1586589209602578E-4	1.5448785612803438E-4	1.5448785612803438E-4	2.5104276620805586E-4	7.509826339557227E-5	0.0
25-29	1.3517687411203008E-4	2.038381435022676E-4	1.8238149681781837E-4	2.982473889138442E-4	7.509826339557227E-5	0.0
30-34	1.8881849082315314E-4	2.3602311352894146E-4	2.145664668444922E-4	3.6476299363563674E-4	8.582658673779689E-5	0.0
35-39	4.291329336889844E-4	2.6606241888717035E-4	2.3816877819738636E-4	4.184046103467598E-4	1.2230288610136055E-4	0.0
40-44	5.321248377743406E-4	3.0468438291917895E-4	2.5104276620805586E-4	4.613179037156582E-4	1.780901674809285E-4	8.582658673779688E-6
45-49	6.758843705601505E-4	3.3901501761429774E-4	2.7679074222939493E-4	4.977942030792219E-4	2.1027513750760237E-4	2.5747976021339063E-5
50-54	7.767306099770619E-4	3.690543229725266E-4	2.8537340090317465E-4	5.621641431325696E-4	2.8537340090317465E-4	3.218497002667383E-5
55-59	9.07616154752202E-4	3.8836530498853085E-4	3.003930535822891E-4	6.436994005334766E-4	3.1326704159295867E-4	4.076762870045352E-5
60-64	0.0010363560348588972	4.291329336889844E-4	3.0897571225606876E-4	6.994866819130445E-4	3.475976762880774E-4	4.935028737423321E-5
65-69	0.001128619615602029	4.699005623894379E-4	3.4116068228274264E-4	7.788762746455067E-4	4.2269593968364964E-4	5.793294604801289E-5
70-74	0.015779217971743958	4.999398677476668E-4	3.540346702934121E-4	8.153525740090704E-4	5.020855324161117E-4	6.651560472179259E-5
75-79	0.028112498485965372	5.407074964481203E-4	3.604716642987469E-4	8.217895680144051E-4	5.750381311432391E-4	7.509826339557227E-5
80-84	0.028245529695408955	5.857664544854637E-4	3.6476299363563674E-4	8.561202027095238E-4	7.896045979877313E-4	7.509826339557227E-5
85-89	0.028299171312120074	6.07223101169913E-4	3.862196403200859E-4	9.119074840890917E-4	8.046242506668458E-4	7.509826339557227E-5
90-94	0.02832706495280986	6.522820592072563E-4	4.0123929299920043E-4	9.462381187842105E-4	8.089155800037357E-4	7.509826339557227E-5
95-99	0.02836783258151031	6.866126939023751E-4	4.2698726902053944E-4	9.998797354953336E-4	8.153525740090704E-4	7.509826339557227E-5
100-104	0.028406454545542324	7.037780112499344E-4	4.505895803734336E-4	0.0010277733761851176	8.282265620197399E-4	7.509826339557227E-5
105-109	0.028421474198221434	7.295259872712735E-4	4.591722390472133E-4	0.0010492300228695669	8.604115320464137E-4	7.509826339557227E-5
110-114	0.02844507650957433	7.552739632926124E-4	4.913572090738871E-4	0.0010964346455753552	8.883051727361978E-4	7.509826339557227E-5
115-119	0.02852232043763835	7.874589333192863E-4	5.321248377743407E-4	0.0011500762622864782	9.505294481211004E-4	7.509826339557227E-5
120-124	0.028784091527188627	8.110612446721805E-4	5.492901551219E-4	0.0011908438909869318	9.93442741489999E-4	8.153525740090704E-5
125-129	0.029372003646342536	8.303722266881849E-4	5.77183795811684E-4	0.0012037178789976012	0.001047084358201122	8.582658673779689E-5
130-134	0.030135860268308928	8.668485260517486E-4	5.900577838223535E-4	0.0012444855076980549	0.0010814149928962406	8.582658673779689E-5
135-139	0.03078170533351085	8.797225140624182E-4	6.029317718330232E-4	0.0012809618070616184	0.0011135999629229144	8.582658673779689E-5
140-144	0.031159342315157157	9.033248254153121E-4	6.115144305068028E-4	0.0013260207650989616	0.0011758242383078173	9.440924541157657E-5
145-149	0.0313717631173332	9.290728014366513E-4	6.243884185174723E-4	0.0014118473518367589	0.0012208831963451607	1.2230288610136055E-4
150-154	0.031676447500252385	9.462381187842105E-4	6.394080711965867E-4	0.0014740716272216615	0.0012659421543825041	1.2873988010669532E-4
155-159	0.032032627835214246	9.655491008002148E-4	6.522820592072562E-4	0.0014826542858954411	0.0013539144057887458	1.2873988010669532E-4
160-162	0.03223145942782347	9.762774241424396E-4	6.544277238757012E-4	0.0014912369445692208	0.0013875298189277164	1.358920956681784E-4
>>END_MODULE
