##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479215
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9407559
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.97544580905631	41.0	40.0	41.0	38.0	41.0
2	40.03908282690547	41.0	40.0	41.0	38.0	41.0
3	40.08087453929335	41.0	40.0	41.0	38.0	41.0
4	40.07124930069532	41.0	40.0	41.0	38.0	41.0
5	40.05435065568018	41.0	40.0	41.0	38.0	41.0
6	40.02410008802496	41.0	40.0	41.0	38.0	41.0
7	39.9861547506638	41.0	40.0	41.0	38.0	41.0
8	39.967533554665984	41.0	40.0	41.0	38.0	41.0
9	39.927619481312846	41.0	40.0	41.0	38.0	41.0
10-14	39.79228741483311	40.4	40.0	41.0	38.0	41.0
15-19	39.556942815878166	40.0	39.0	41.0	37.0	41.0
20-24	39.4486331470257	40.0	39.0	41.0	36.8	41.0
25-29	39.39227487172815	40.0	39.0	41.0	36.0	41.0
30-34	39.15623757448664	40.0	38.2	41.0	35.2	41.0
35-39	38.67401488526407	40.0	37.4	41.0	35.0	41.0
40-44	38.027794011177605	39.2	35.6	41.0	34.0	41.0
45-49	37.30074860014165	37.8	35.0	40.0	33.2	41.0
50-54	36.65863907948916	36.6	35.0	39.6	33.0	41.0
55-59	36.06154248939603	35.6	34.8	38.8	33.0	40.6
60-64	35.38325888787942	35.0	33.8	37.0	32.4	39.8
65-69	35.37825988654443	35.0	34.0	36.8	33.0	40.0
70-74	35.17225603368525	35.0	34.0	36.0	32.0	40.0
75-79	35.126835452214536	35.0	34.0	36.0	32.0	40.0
80-84	35.19346198094532	35.0	34.0	36.0	32.0	40.0
85-89	38.571504871773854	39.8	37.8	40.2	35.2	41.0
90-94	39.49368249890365	40.6	39.0	41.0	36.4	41.0
95-99	39.275044932830184	40.0	38.6	41.0	35.6	41.0
100-104	39.006328162102754	40.0	38.0	41.0	35.0	41.0
105-109	38.69984297308788	40.0	38.0	41.0	34.8	41.0
110-114	38.44746286044566	40.0	37.0	41.0	34.0	41.0
115-119	38.45496908843893	40.0	37.0	41.0	34.0	41.0
120-124	37.84433705868169	39.2	35.6	40.6	33.6	40.8
125-129	37.75270814992562	39.0	35.0	40.8	33.4	41.0
130-134	37.27318062327613	38.0	35.0	40.4	33.0	41.0
135-139	36.67385187834476	36.6	35.0	39.6	33.0	41.0
140-144	36.03107501986162	35.6	34.8	38.8	32.8	40.6
145-149	35.40023721385707	35.0	34.0	37.0	32.0	39.4
150-154	34.8482678459275	35.0	34.0	36.0	32.0	38.0
155-159	34.40214685908679	35.0	34.0	35.2	31.2	36.8
160-164	34.103965912411674	35.0	34.0	35.0	31.0	36.0
165-169	33.90353912587541	35.0	34.0	35.0	31.0	35.6
170-173	33.571969652740435	34.75	33.0	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	65.0
31	1048.0
32	9902.0
33	56391.0
34	241857.0
35	823196.0
36	1996005.0
37	3088653.0
38	2573974.0
39	609428.0
40	7039.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.20087059778206	27.01529695428963	28.248549916083437	22.53528253184487
2	22.18709444182067	27.184139902816444	28.07648615331565	22.552279502047238
3	22.04048893023153	27.564961325249193	27.89005096858813	22.504498775931143
4	22.139760165203324	27.69756745612757	27.71906081056733	22.44361156810178
5	22.444993435597908	27.68860657690268	27.46849634426954	22.397903643229874
6	22.04011688898257	27.792257268862198	27.651423711506883	22.516202130648345
7	22.156087461157565	27.75860348045651	27.662085350727004	22.423223707658916
8	22.55808334553097	27.58364842569683	27.434863815363798	22.42340441340841
9	22.170671478116695	27.611253886369465	27.4653711977783	22.75270343773555
10-14	22.30560552423854	27.595528234263533	27.538148843924336	22.56071739757359
15-19	22.176715553949755	27.508064525558652	27.675470331889496	22.6397495886021
20-24	22.33141030526622	27.39338865693003	27.522189337319062	22.753011700484684
25-29	22.375153852343633	27.486705105968507	27.451867163416143	22.686273878271717
30-34	22.276660715069657	27.429906099977686	27.53319112853823	22.76024205641442
35-39	22.30506765889005	27.474317195353226	27.531987840841605	22.688627304915123
40-44	22.43198899948435	27.345661079563783	27.382631349960175	22.83971857099169
45-49	22.627049163337695	27.296606909401262	27.264768682290487	22.811575244970562
50-54	22.625592887591775	27.277267142305462	27.261992191598267	22.835147778504496
55-59	22.546331093963907	27.359496762125012	27.243258320250767	22.850913823660314
60-64	22.372964123849766	27.384587223954693	27.359921952123816	22.882526700071722
65-69	22.396419730134035	27.405408778196342	27.309143636516126	22.8890278551535
70-74	22.300401198653123	27.440380655598336	27.34202783102397	22.917190314724575
75-79	22.19178853940751	27.462624470385993	27.386634513798956	22.958952476407536
80-84	22.151369978120787	27.469795299715898	27.395995071622725	22.982839650540594
85-89	21.74624044345616	27.475694810949364	28.32709101266333	22.450973732931146
90-94	22.161121148790162	27.835119993890554	27.49084056322335	22.512918294095932
95-99	22.296475696351077	27.836347461929478	27.310726013759474	22.55645082795997
100-104	22.22735225645901	27.824594959748854	27.384777122562014	22.563275661230122
105-109	22.278320715879193	27.69273488169143	27.39097234738112	22.637972055048262
110-114	22.369688852502538	27.740580498955243	27.253721752476466	22.636008896065757
115-119	22.32594537938676	27.7912643652641	27.287461138782515	22.595329116566624
120-124	22.339262265695563	27.799475851399457	27.33738653815537	22.5238753447496
125-129	22.34803063109286	27.852578206181406	27.337217764475607	22.462173398250126
130-134	22.402305249451675	27.85852337739151	27.309303500895165	22.429867872261653
135-139	22.331719048068052	27.995261249692287	27.409819387651176	22.26320031458848
140-144	22.179210847200384	28.146046478127307	27.51321666804527	22.161526006627042
145-149	21.96723385874548	28.349312228732725	27.65899334850707	22.02446056401472
150-154	21.843051153205867	28.491713941427538	27.80045272529331	21.864782180073284
155-159	21.639311312222194	28.74103010740008	27.95748627103106	21.662172309346676
160-164	21.366588852292153	28.93014675261823	28.173549260433266	21.52971513465635
165-169	21.189034358676196	29.185415537929476	28.36005246601329	21.26549763738104
170-173	20.89232537428065	29.430396191420343	28.580623197365806	21.096655236933202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	3.5
9	6.0
10	22.5
11	51.0
12	103.0
13	211.5
14	400.0
15	768.5
16	1417.0
17	2399.5
18	3901.0
19	6195.5
20	9582.5
21	14161.5
22	20024.0
23	27257.5
24	36318.5
25	46992.0
26	59056.5
27	73144.0
28	88385.0
29	104052.5
30	121155.5
31	139648.5
32	159647.5
33	180759.0
34	202134.0
35	224959.0
36	248221.0
37	271297.5
38	294955.0
39	318362.5
40	338901.0
41	356337.5
42	371133.0
43	381320.5
44	385656.5
45	384500.0
46	378737.5
47	367162.0
48	350017.0
49	329715.5
50	307264.0
51	284164.5
52	260540.5
53	237554.0
54	216831.5
55	198250.5
56	182670.0
57	169300.0
58	157552.5
59	147419.5
60	137825.0
61	127682.5
62	117351.5
63	106365.0
64	94187.5
65	81674.5
66	68696.0
67	56083.5
68	44756.0
69	34372.0
70	25526.5
71	18427.0
72	12899.0
73	8664.5
74	5563.5
75	3580.5
76	2259.0
77	1315.0
78	717.0
79	388.5
80	213.5
81	114.0
82	49.0
83	22.5
84	10.5
85	3.5
86	1.0
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2306.0
92-93	5503.0
94-95	8922.0
96-97	12251.0
98-99	15772.0
100-101	19518.0
102-103	23284.0
104-105	27178.0
106-107	30650.0
108-109	34144.0
110-111	37384.0
112-113	41079.0
114-115	44701.0
116-117	48559.0
118-119	52312.0
120-121	55993.0
122-123	60254.0
124-125	63758.0
126-127	68251.0
128-129	73162.0
130-131	127000.0
132-133	149259.0
134-135	167257.0
136-137	173853.0
138-139	178000.0
140-141	178105.0
142-143	182995.0
144-145	187932.0
146-147	191435.0
148-149	188648.0
150-151	189962.0
152-153	193804.0
154-155	206354.0
156-157	206868.0
158-159	209856.0
160-161	215090.0
162-163	222335.0
164-165	228458.0
166-167	232272.0
168-169	240278.0
170-171	248399.0
172-173	4564418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.90361668900063
#Duplication Level	Percentage of total
1	92.28273524304498
2	6.552878347879985
3	0.800172883301765
4	0.21635889323606142
5	0.06127434179572673
6	0.033882024970241426
7	0.015483546715917618
8	0.012453171383435477
9	0.005216557643324398
>10	0.019544990028531075
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.125949994042025E-5	0.0	0.0
2	0.0	2.125949994042025E-5	2.125949994042025E-5	2.125949994042025E-5	1.0629749970210125E-5	0.0
3	0.0	2.125949994042025E-5	2.125949994042025E-5	4.25189998808405E-5	1.0629749970210125E-5	0.0
4	0.0	3.188924991063038E-5	2.125949994042025E-5	5.314874985105063E-5	1.0629749970210125E-5	0.0
5	0.0	3.188924991063038E-5	2.125949994042025E-5	8.5037999761681E-5	1.0629749970210125E-5	0.0
6	0.0	4.25189998808405E-5	2.125949994042025E-5	9.566774973189113E-5	2.125949994042025E-5	0.0
7	0.0	6.377849982126075E-5	4.25189998808405E-5	1.0629749970210126E-4	2.125949994042025E-5	0.0
8	0.0	7.440824979147088E-5	4.25189998808405E-5	1.0629749970210126E-4	3.188924991063038E-5	0.0
9	0.0	7.440824979147088E-5	5.314874985105063E-5	1.275569996425215E-4	3.188924991063038E-5	0.0
10-14	0.0	1.0629749970210126E-4	6.165254982721873E-5	1.5519434956506783E-4	5.314874985105063E-5	0.0
15-19	1.0629749970210125E-5	1.0629749970210126E-4	7.866014977955492E-5	1.955873994518663E-4	8.5037999761681E-5	0.0
20-24	1.275569996425215E-5	1.339348496246476E-4	9.566774973189113E-5	2.3385449934462278E-4	8.716394975572302E-5	0.0
25-29	3.826709989275645E-5	2.2109879938037063E-4	1.2330509965443747E-4	3.125146491241777E-4	1.275569996425215E-4	0.0
30-34	9.991964971997518E-5	2.6361779926121115E-4	1.339348496246476E-4	3.337741490645979E-4	1.5519434956506786E-4	0.0
35-39	2.57239949279085E-4	2.9763299916588353E-4	1.5306839957102584E-4	4.018045488739428E-4	1.70075999523362E-4	2.976329991658835E-5
40-44	3.571595989990602E-4	3.188924991063038E-4	1.6369814954123594E-4	4.464494987488253E-4	1.9346144945782428E-4	5.314874985105063E-5
45-49	4.99598248599876E-4	3.6353744898118625E-4	1.8070574949357213E-4	4.7408684867137165E-4	2.3810639933270682E-4	7.228229979742885E-5
50-54	6.058957483019772E-4	4.0605644886202683E-4	2.1684689939228656E-4	5.10227998570086E-4	2.763734992254633E-4	9.779369972593315E-5
55-59	6.930596980577002E-4	4.868425486356237E-4	2.6361779926121115E-4	5.825102983675149E-4	3.826709989275645E-4	1.1692724967231139E-4
60-64	7.823495978074652E-4	5.378653484926323E-4	2.933810991777995E-4	6.441628481947336E-4	5.463691484688005E-4	1.1692724967231139E-4
65-69	8.610097475870201E-4	5.421172484807164E-4	3.0826274913609366E-4	7.058153980219522E-4	6.229033482543134E-4	1.2330509965443747E-4
70-74	0.01598501800520199	5.569988984390105E-4	3.3164819907055586E-4	7.610900978670449E-4	6.696742481232379E-4	1.275569996425215E-4
75-79	0.027907345571789664	5.76132448385389E-4	3.486557990228921E-4	8.036090977478856E-4	7.206970479802465E-4	1.275569996425215E-4
80-84	0.028009391171503684	6.271552482423976E-4	3.6778934896927035E-4	8.52505947610852E-4	9.481736973427432E-4	1.275569996425215E-4
85-89	0.028030650671444102	6.590444981530277E-4	3.720412489573544E-4	8.886470975095664E-4	9.928186472176258E-4	1.275569996425215E-4
90-94	0.02806891777133686	7.058153980219524E-4	3.7629314894543843E-4	9.396698973665751E-4	9.991964971997518E-4	1.275569996425215E-4
95-99	0.028117814621199822	7.547122478849189E-4	3.826709989275645E-4	9.630553473010374E-4	0.0010098262471699617	1.275569996425215E-4
100-104	0.028175215271038962	7.823495978074652E-4	4.3794569877265716E-4	0.001005574347181878	0.0010268338471222983	1.275569996425215E-4
105-109	0.028241119720854264	7.929793477776754E-4	4.847165986415818E-4	0.0010459673970686762	0.0010502192970567605	1.275569996425215E-4
110-114	0.028296394420699356	8.312464476704319E-4	5.059760985820019E-4	0.0011203756468601473	0.0010863604469554748	1.275569996425215E-4
115-119	0.028347417220556365	8.716394975572305E-4	5.10227998570086E-4	0.0011692724967231139	0.0011161237468720632	1.275569996425215E-4
120-124	0.028659931869680542	9.099065974499868E-4	5.229836985343382E-4	0.0012224212465741647	0.001169272496723114	1.275569996425215E-4
125-129	0.029297716867893148	9.247882474082809E-4	5.421172484807164E-4	0.0012755699964252152	0.0012096655466099125	1.275569996425215E-4
130-134	0.030035421515825732	9.630553473010374E-4	5.506210484568846E-4	0.0013478522962226437	0.0012670661964490472	1.275569996425215E-4
135-139	0.03067320651403834	0.0010034483971878357	5.527469984509265E-4	0.0014010010460736946	0.0013202149463000976	1.275569996425215E-4
140-144	0.031021862313061234	0.0010162040971520882	5.527469984509265E-4	0.0014371421959724089	0.0013924972460975264	1.275569996425215E-4
145-149	0.031225953512489264	0.001020455997140172	5.697545984032628E-4	0.0014605276459068715	0.0014477719459426192	1.275569996425215E-4
150-154	0.03153421626162536	0.0010480933470627183	5.761324483853889E-4	0.0014860390458353756	0.0014732833458711233	1.275569996425215E-4
155-159	0.03195728031043972	0.0010736047469912227	5.846362483615569E-4	0.001520054245740048	0.0014966687958055855	1.275569996425215E-4
160-162	0.0322294019096771	0.0010771479969812927	5.846362483615569E-4	0.0015413137456804684	0.0015377704956903982	1.275569996425215E-4
>>END_MODULE
