##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479216
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9499876
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.95151547241248	41.0	40.0	41.0	38.0	41.0
2	40.0236646246751	41.0	40.0	41.0	38.0	41.0
3	40.077894069354166	41.0	40.0	41.0	38.0	41.0
4	40.07445549815598	41.0	40.0	41.0	38.0	41.0
5	40.05598778341949	41.0	40.0	41.0	38.0	41.0
6	40.02278124472362	41.0	40.0	41.0	38.0	41.0
7	39.984122634863866	41.0	40.0	41.0	38.0	41.0
8	39.96620608521627	41.0	40.0	41.0	38.0	41.0
9	39.92091917831348	41.0	40.0	41.0	38.0	41.0
10-14	39.793353829039454	40.4	40.0	41.0	38.0	41.0
15-19	39.56652331040952	40.0	39.0	41.0	37.0	41.0
20-24	39.4618491651891	40.0	39.0	41.0	36.8	41.0
25-29	39.397199773976	40.0	39.0	41.0	36.0	41.0
30-34	39.15068192469039	40.0	38.2	41.0	35.2	41.0
35-39	38.67170024113999	40.0	37.2	41.0	35.0	41.0
40-44	38.02427570633554	39.2	35.6	41.0	34.0	41.0
45-49	37.296013569019216	37.6	35.0	40.0	33.2	41.0
50-54	36.657927682424486	36.6	35.0	39.4	33.0	41.0
55-59	36.0484524429582	35.4	34.8	38.6	33.0	40.4
60-64	35.37787604806631	35.0	33.8	37.0	32.4	39.8
65-69	35.35727321072402	35.0	34.0	36.6	33.0	39.4
70-74	35.15041857388455	35.0	34.0	36.0	32.4	39.6
75-79	35.08263131013499	35.0	34.0	36.0	32.0	40.0
80-84	35.14905577714909	35.0	34.0	36.0	32.0	40.0
85-89	38.555614641706896	39.8	37.8	40.2	35.2	41.0
90-94	39.50334380521501	40.6	39.0	41.0	36.4	41.0
95-99	39.29021495672132	40.0	38.8	41.0	35.6	41.0
100-104	39.026646482880835	40.0	38.0	41.0	35.0	41.0
105-109	38.722245870158936	40.0	38.0	41.0	34.8	41.0
110-114	38.46660917703764	40.0	37.0	41.0	34.0	41.0
115-119	38.467214888378926	40.0	37.0	41.0	34.0	41.0
120-124	37.858192742619885	39.2	35.6	40.6	33.6	40.8
125-129	37.755349176794674	39.0	35.0	40.8	33.6	41.0
130-134	37.26350004549437	37.8	35.0	40.4	33.0	41.0
135-139	36.66745247401489	36.6	35.0	39.6	33.0	41.0
140-144	36.019955986757395	35.4	34.8	38.6	33.0	40.6
145-149	35.38854140099351	35.0	34.0	37.0	32.0	39.2
150-154	34.83948541263394	35.0	34.0	36.0	32.0	38.0
155-159	34.407252816988226	35.0	34.0	35.2	31.4	36.6
160-164	34.10327216786851	35.0	34.0	35.0	31.0	36.0
165-169	33.89959405291817	35.0	34.0	35.0	31.0	35.4
170-173	33.572250292203655	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	72.0
31	1178.0
32	10128.0
33	58507.0
34	250455.0
35	843948.0
36	2022066.0
37	3101473.0
38	2595662.0
39	609520.0
40	6864.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.236058660134088	27.008773588202622	28.19794700478196	22.557220746881327
2	22.231237544574267	27.125122475282836	28.054692503354783	22.588947476788118
3	22.112962316560765	27.513506492084737	27.84832138861602	22.525209802738477
4	22.19565813280089	27.623655298237576	27.696508880747494	22.484177688214036
5	22.453756238502482	27.660634728284872	27.422063193245886	22.463545839966752
6	22.096867369637245	27.779678387381058	27.586296915875536	22.537157327106165
7	22.191952821278928	27.741172621621583	27.61452886332411	22.45234569377537
8	22.638716547458092	27.50738009632968	27.378578415128786	22.475324941083443
9	22.2017424227432	27.569475643682082	27.42436848649393	22.804413447080783
10-14	22.369035132669097	27.54471321520407	27.485571390616048	22.60068026151078
15-19	22.229677524211894	27.459562630080647	27.651527240987146	22.659232604720316
20-24	22.40363347900541	27.360197122572966	27.45993316123284	22.77623623718878
25-29	22.4400844811027	27.432069639645825	27.417496817853205	22.710349061398276
30-34	22.332386233251885	27.397791297486407	27.47990394821996	22.78991852104175
35-39	22.357868671127918	27.42225687998454	27.48706614696865	22.73280830191889
40-44	22.47699864714024	27.308924874387834	27.352801236563508	22.861275241908423
45-49	22.65633993538442	27.27296651029971	27.225681682581964	22.845011871733906
50-54	22.664413724979145	27.225584839212637	27.243844024911485	22.866157410896733
55-59	22.58346319467749	27.32391243843604	27.21423732267663	22.87838704420984
60-64	22.422018982142504	27.34519692678094	27.328047229248043	22.90473686182851
65-69	22.436482328821977	27.38680378564941	27.275105485587392	22.90160839994122
70-74	22.363891907641744	27.39381861405349	27.318472367428797	22.923817110875973
75-79	22.250252529611966	27.42546955349733	27.340744237082674	22.983533679808033
80-84	22.208178296221973	27.43775181907638	27.36621614850552	22.987853736196133
85-89	21.7929897190237	27.40580613894329	28.31114006119659	22.490064080836422
90-94	22.21007180899831	27.776733342053383	27.46920943932811	22.54398540962019
95-99	22.345878701906894	27.783517390506052	27.288499444459557	22.5821044631275
100-104	22.285305690177502	27.765848517896853	27.35262685721264	22.596218934713008
105-109	22.32109905474864	27.644105598773557	27.36019297611988	22.674602370357928
110-114	22.41447936524733	27.695524294476776	27.223682599910692	22.666313740365197
115-119	22.375916493575325	27.706989824069694	27.28212649178832	22.63496719056666
120-124	22.376813865979578	27.744719011167255	27.311865300037535	22.56660182281563
125-129	22.410005960242138	27.790844760456583	27.300965008560325	22.498184270740957
130-134	22.453423078738698	27.79564114579675	27.279222042736528	22.47171373272802
135-139	22.373916056579795	27.9404363735049	27.355586000609033	22.33006156930627
140-144	22.239329917477697	28.0695562099745	27.46790356296829	22.223210309579517
145-149	22.04945684301572	28.249076339961466	27.618500346303147	22.08296647071967
150-154	21.910271399394667	28.401652107980386	27.763014257740693	21.92506223488425
155-159	21.712516797341227	28.665972381757783	27.906559558517092	21.7149512623839
160-164	21.438012750391223	28.838470557585172	28.13054681295748	21.592969879066125
165-169	21.275037161946695	29.09477618075842	28.305601487343303	21.324585169951586
170-173	20.950615052704734	29.34706824939008	28.55812473643408	21.144191961471105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	4.0
8	7.5
9	10.0
10	22.5
11	54.0
12	112.0
13	224.5
14	406.5
15	737.5
16	1380.0
17	2392.5
18	4026.0
19	6313.5
20	9572.5
21	14197.5
22	19946.5
23	27361.0
24	36464.5
25	46816.0
26	58955.0
27	72719.5
28	87834.0
29	104414.0
30	121618.5
31	140067.5
32	160242.5
33	180971.5
34	202726.0
35	225844.0
36	249634.0
37	273404.5
38	296970.0
39	320006.0
40	340776.5
41	358817.5
42	373541.0
43	383451.0
44	388239.5
45	387002.0
46	380521.5
47	369066.5
48	352444.0
49	332549.5
50	310255.5
51	286767.0
52	263599.0
53	240975.5
54	219947.5
55	201386.5
56	185787.5
57	172497.0
58	160395.0
59	150003.0
60	140504.0
61	130972.0
62	120556.5
63	108719.5
64	96413.5
65	84023.0
66	71148.0
67	58599.5
68	46729.0
69	35946.5
70	26898.5
71	19478.0
72	13640.0
73	9131.5
74	5986.5
75	3928.5
76	2434.0
77	1399.0
78	791.0
79	420.5
80	223.0
81	110.5
82	45.5
83	19.5
84	9.0
85	5.0
86	2.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2214.0
92-93	5306.0
94-95	8390.0
96-97	11575.0
98-99	14758.0
100-101	18394.0
102-103	22014.0
104-105	25574.0
106-107	28777.0
108-109	31828.0
110-111	34943.0
112-113	38394.0
114-115	41894.0
116-117	44823.0
118-119	48474.0
120-121	52569.0
122-123	56373.0
124-125	60533.0
126-127	64867.0
128-129	69399.0
130-131	128732.0
132-133	150227.0
134-135	165987.0
136-137	170352.0
138-139	175054.0
140-141	175574.0
142-143	180623.0
144-145	188585.0
146-147	190745.0
148-149	185426.0
150-151	190147.0
152-153	200886.0
154-155	198470.0
156-157	203341.0
158-159	205746.0
160-161	218450.0
162-163	225560.0
164-165	230899.0
166-167	236876.0
168-169	239058.0
170-171	247956.0
172-173	4710083.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.05813007878842
#Duplication Level	Percentage of total
1	92.58448776948221
2	6.292260093202541
3	0.7596596290053228
4	0.1797537474584662
5	0.08065497024580733
6	0.03284348673790853
7	0.026826482883244678
8	0.010372355155871772
9	0.0031281062834852117
>10	0.030013359545123238
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1052906374777943E-5	2.1052906374777943E-5	0.0	2.1052906374777943E-5	0.0	0.0
2	3.1579359562166916E-5	3.1579359562166916E-5	1.0526453187388972E-5	3.1579359562166916E-5	0.0	0.0
3	3.1579359562166916E-5	4.2105812749555886E-5	2.1052906374777943E-5	3.1579359562166916E-5	0.0	0.0
4	3.1579359562166916E-5	6.315871912433383E-5	2.1052906374777943E-5	3.1579359562166916E-5	2.1052906374777943E-5	0.0
5	3.1579359562166916E-5	6.315871912433383E-5	2.1052906374777943E-5	7.36851723117228E-5	3.1579359562166916E-5	0.0
6	4.2105812749555886E-5	7.36851723117228E-5	3.1579359562166916E-5	9.473807868650076E-5	3.1579359562166916E-5	0.0
7	5.263226593694486E-5	8.421162549911177E-5	4.2105812749555886E-5	9.473807868650076E-5	4.2105812749555886E-5	0.0
8	6.315871912433383E-5	8.421162549911177E-5	4.2105812749555886E-5	9.473807868650076E-5	4.2105812749555886E-5	0.0
9	6.315871912433383E-5	9.473807868650076E-5	4.2105812749555886E-5	9.473807868650076E-5	5.263226593694486E-5	0.0
10-14	8.210633486163398E-5	1.2631743824866767E-4	4.8421684661989276E-5	1.115804037863231E-4	5.894813784937824E-5	0.0
15-19	1.2631743824866767E-4	1.768444135481347E-4	6.105342848685604E-5	1.494756352609234E-4	7.57904629492006E-5	0.0
20-24	1.3473860079857885E-4	2.1263435438525726E-4	7.36851723117228E-5	2.1473964502273504E-4	9.473807868650077E-5	0.0
25-29	1.4105447271101223E-4	2.5263487649733533E-4	9.052749741154516E-5	2.4842429522237976E-4	1.094751131488453E-4	0.0
30-34	2.042131918353461E-4	2.778983641470689E-4	9.684336932397855E-5	3.4105708327140266E-4	1.2842272888614545E-4	0.0
35-39	4.084263836706921E-4	3.1368830498419134E-4	1.157909850612787E-4	4.105316743081699E-4	1.7894970418561251E-4	0.0
40-44	5.726390533939602E-4	3.389517926339249E-4	1.3894918207353445E-4	4.631639402451148E-4	2.4000313267246858E-4	4.210581274955588E-6
45-49	7.221146886548836E-4	3.768470241085252E-4	1.5789679781083458E-4	4.968485904447595E-4	3.221094675341026E-4	3.78952314746003E-5
50-54	9.347490430401407E-4	4.0000522112078096E-4	1.5789679781083458E-4	5.494808563817044E-4	3.8737347729591416E-4	5.263226593694486E-5
55-59	0.0010589611906513309	4.1263696494564766E-4	1.8105499482309032E-4	5.957972504062159E-4	4.5053219642024803E-4	6.526400976181163E-5
60-64	0.0011136987472257533	4.3579516195790347E-4	2.315819701225574E-4	6.484295163431606E-4	5.305332406444043E-4	8.421162549911177E-5
65-69	0.001195805082087387	4.5474277769520354E-4	2.505295858598575E-4	7.136935261049724E-4	6.084289942310825E-4	9.894865996145635E-5
70-74	0.017244435611580615	4.968485904447595E-4	2.6737191095967995E-4	7.642205014044394E-4	6.905353290927167E-4	1.1789627569875648E-4
75-79	0.030446713199203862	5.326385312818821E-4	2.863195266969801E-4	8.084316047914731E-4	8.042210235165174E-4	1.3473860079857885E-4
80-84	0.03066566342550155	5.557967282941378E-4	2.947406892468912E-4	8.947485209280625E-4	0.00100211834343943	1.3684389143605664E-4
85-89	0.030739348597813278	5.915866691312602E-4	2.989512705218468E-4	9.136961366653627E-4	0.0010315924123641192	1.3684389143605664E-4
90-94	0.030800402026300133	6.273766099683827E-4	3.094777237092358E-4	9.368543336776185E-4	0.0010421188655515082	1.3684389143605664E-4
95-99	0.030859350164149514	6.736930039928943E-4	3.221094675341025E-4	9.600125306898742E-4	0.0010821193876635864	1.3684389143605664E-4
100-104	0.03089092952371168	7.326411418422725E-4	3.389517926339249E-4	9.894865996145634E-4	0.0011010670034008866	1.3684389143605664E-4
105-109	0.030951982952198538	7.89483989054173E-4	3.6210998964618065E-4	0.0010358029936390748	0.001136856944238009	1.3684389143605664E-4
110-114	0.031000404636860525	8.084316047914731E-4	3.8737347729591416E-4	0.0011094881659507979	0.0011789627569875648	1.3684389143605664E-4
115-119	0.031044615740247555	8.61063870728418E-4	4.0000522112078096E-4	0.0011705415944376538	0.0013137013577861436	1.3684389143605664E-4
120-124	0.031421462764356076	8.821167771031959E-4	4.1263696494564777E-4	0.0012337003135619875	0.0014105447271101222	1.3684389143605664E-4
125-129	0.03212462983727367	8.947485209280625E-4	4.442163245078146E-4	0.001261069091849199	0.001465282283684545	1.3684389143605664E-4
130-134	0.03270779534385501	8.947485209280625E-4	4.6737452152007035E-4	0.0012947537420488434	0.0015347568747213123	1.4526505398596782E-4
135-139	0.033229907421949506	9.052749741154515E-4	4.8211155598241486E-4	0.0013368595547983994	0.0015831785593833016	1.515809258984012E-4
140-144	0.03357938566777082	9.305384617651852E-4	5.01059171719715E-4	0.0013768600769104775	0.0016442319878701573	1.5789679781083458E-4
145-149	0.03376254595323139	9.452754962275296E-4	5.073750436321485E-4	0.0014315976334849003	0.0017137065789069247	1.6210737908579014E-4
150-154	0.03404676018929089	9.663284026023077E-4	5.284279500069265E-4	0.0014715981555969784	0.0017368647759191803	1.7894970418561251E-4
155-159	0.03434992204108769	9.958024715269968E-4	5.389544031943154E-4	0.001515809258984012	0.0017579176822939582	1.7894970418561251E-4
160-162	0.0346530838928845	0.0010386100478223786	5.473755657442265E-4	0.001515809258984012	0.0017789705886687363	1.8596733964387183E-4
>>END_MODULE
